Comparative analysis of essential oils found in Rhizomes Curcumae and Radix Curcumae by gas chromatography-mass spectrometry

A comparison of the volatile compounds in Rhizomes Curcumae （Ezhu） and Radix Curcumae （Yujin） was undertaken using gas chromatography mass spectrometi-y （GC-MS）. Ultrasonic extraction and GC-MS methods were developed for the simultaneous determination of five sesquiterpenes, namely, α-pinene, β-elemene, curcumol, germacrone and curdione, in Ezhu and Yunjin. Good linearity （r〉0.999） and high inter-day precision were observed over the investigated concentration ranges. The validated method was successfully used for the simultaneous determination of five sesquiterpenes in Ezhu and Yujin. The quantitative method can be effectively used to evaluate and monitor the quality of Chinese curcuma in clinical use.

Effects of radix curcumae-derived diterpenoid C on Helicobacter pylori-induced inflammation and nuclear factor kappa B signal pathways

AIM:To study effect of diterpenoid C extracted from radix curcumae on Helicobacter pylori(H.pylori)-infected inflammation,intestinal metaplasia,and nuclear factor kappa B(NF-κB)signaling pathway in vitro.METHODS:We used I-type H.pylori to infect human gastric epithelial gastric epithelium cell line(GES-1)cell lines,and then H.pylori-infected GES-1 cells were treated with radix curcumae(RC)-derived diterpenoid C of different concentrations(5,10,20μg/mL)and amoxicillin.The expression of p65,IκB kinase(IKK)αand IKKγproteins was detected with Western blotting,and the expression of interleukin(IL)-8,IL-6 and IL-4 was determined with enzyme-linked immunosorbent assay method.Data were analyzed using SPSS software ver18.0.For comparisons between groups of more than two unpaired values,one-way analysis of variance(ANOVA)was used.If an ANOVA F value was significant,post hoc comparisons were performed between groups.If results were not normally distributed,the Mann-Whitney U test was used to compare two groups of unpaired values,whereas for comparisons between groups of more than two unpaired values,the Kruskal-Wallis H test was used.Statistical significance was established at P<0.05.RESULTS:The MTT assay results revealed the inhibited rate of GES-1,and indicated that the IC5 of RCderived diterpenoid C and amoxicillin all were 5μg/mL for gastric GES-1 cells.The expression of IL-8 was significantly increased,especially at 12 h time point;and the expression of IL-4 was decreased in H.pyloriinfected GES-1 cells.After H.pylori-infected GES-1 cells were treated with RC-derived diterpenoid C of different concentrations and amoxicillin,the expression of IL-8was decreased at 12,24,48,72 h points(P<0.01),especially in high-concentration diterpenoid C(20μg/mL)group;and the expression of IL-4 was increased,especially in moderate and high-concentration diterpenoid C(10 and 20μg/mL)groups.RC-derived diterpenoid C had the inhibitory effects on H.pylori-induced p65 translocation from cytoplasm into cell nucleus,H.pylori-stimulant IkBαdegradation,the phosphorylation of p65 and IkBα,and the expression of IKKαand IKKβproteins.CONCLUSION:RC-derived diterpenoid C can block NF-κB signal pathway,effectively reducing the secretion of H.pylori-induced proinflammatory cytokine and increasing the secretion of anti-inflammatory cytokine.

STUDY OF THE MECHANISM OF ARTEMISINE CAPILLARIES AND RADIX CURCUMAE PREVENTING GALLSTONE FORMATION INDUCED BY HIGH CHOLESTEROL DIET IN GUINEA PIGS

To study the effect of chinese herbal medicine preventing gallstone formation, morphology of livers and gallbladders as well as conjugated bile acids of the bile. 58 female guinea pigs were randomly divided into three groups. The control group was fed on normal diet; the lithogenous groups was high cholesterol diet (HCD) and the antilithic group was high cholesterol and the chinese herbal medicine capsule (13 5 g/kg). 8 weeks later the guinea pigs were killed. Liver and gallbladder were removed for light and electroscope observation. Gallbladder bile were withdrawn and gallbladder were collected. The minute construction of gallstones were observed by scanning electron microscope and gallstone components were quantified by Fourier Transform Infrand Spectros Copy (FT IR). The morphology of liver and gallbladder were observed by light microscope and transmission electron microscope. Conjugated bile acid of biles were determined by Thin layer Chromatograph. The effect of chinese herbal medicine preventing gallstone formation, morphololgy of livers and gallbladders as well as conjugated bile acids of the bile were also observed. We measured conjugated bile acids in all groups and found that decreased in lithogenic group and increased notably in antilithic group (P<0 01) . The gallstone formation rate was 100% when the animals were fed on HCD in lithogenic group. The rate decreased to 5 3% when the animals were fed on HCD and Chinese herbal medicine capsule inantilithic group. It indicates that changes in diet is an important factor in the process of gallstone formation and the chinese herbal medicine can prevent gallstone formation effectively.

Network pharmacology-based analysis on the anti-hepatocellular carcinoma effect of Curcumae Radix

As a traditional Chinese medicine,Curcumae Radix(CR)has exhibited anti-hepatocellular carcinoma(HCC)activity.However,the underlying molecular mechanism is still unclear.In the present study,network pharmacology was utilized to reveal the mechanism of the effects of CR in HCC.The analysis of the network results showed that the active components of CR,including curcumin,naringenin,sitosterol,demethoxycurcumin,andβ-elemene,were closely related to the screened hub-targets,such as AKT1,TP53,MAPK8,MAPK1,JUN,STAT3,VEGFA,MAPK3,IL6,TNF,CCND1,EP300,EGFR,and ESR1.GO and KEGG analyses revealed that these targets were associated with pathways in cancer,proteoglycans in cancer,PI3K-Akt signaling pathway,AGE-RAGE signaling pathway in diabetic complications,MAPK signaling pathway,hepatitis B,hepatitis C,and other biological processes.The candidate active components of CR(curcumin and naringenin)prominently exhibited their antitumor effects by regulating PI3K-Akt signaling pathway,MAPK signaling pathway,hepatitis B and hepatitis C.This study successfully predicted,explained,and confirmed the multi-component,multi-target,and multi-channel characteristics of CR,which not only gave novel insights into the pharmacological and biological molecular mechanism of CR applying to HCC disease,but also provided a feasible method for discovering potential activated compounds from Chinese herbs.

Essential oil of Curcuma wenyujin induces apoptosis in human hepatoma cells

AIM: To investigate the effects of the essential oil of Curcuma wenyujin (CWO) on growth inhibition and on the induction of apoptosis in human HepG2 cancer cells. METHODS: The cytotoxic effect of drugs on HepG2 cells was measured by 3-(4,5-dimethylthiazol-2- yl)-2,5-diphenyltetra-zolium bromide (MTT) assay. DNA fragmentation was visualized by agarose gel electrophoresis. Cell cycle and mitochondrial transmembrane potential (△Ψm) were determined by flow cytometry (FCM). Cytochrome C immunostaining was evaluated by fluorescence microscopy. Caspase-3 enzymatic activity was assayed by the cleavage of Ac-DEVD-R110. Cleaved PARP and active caspase-3 protein levels were measured by FCM using BD? CBA Human Apoptosis Kit. RESULTS: Treatment with CWO inhibited the growth of HepG2 cells in a dose-dependent manner, and the IC50 of CWO was approximately 70 μg/mL. CWO was found to inhibit the growth of HepG2 cells by inducing a cell cycle arrest at S/G2. DNA fragmentation was evidentlyobserved at 70 μg/mL after 72 h of treatment. During the process, cytosolic HepG2 cytochrome C staining showed a markedly stronger green fluorescence than in control cells in a dose-dependent fashion, and CWO also caused mitochondrial transmembrane depolarization. Furthermore, the results clearly demonstrated that both, activity of caspase-3 enzyme and protein levels of cleaved PARP, significantly increased in a dose- dependent manner after treatment with CWO. CONCLUSION: CWO exhibits an antiproliferative effect in HepG2 cells by inducing apoptosis. This growth inhibition is associated with cell cycle arrest, cytochrome C translocation, caspase 3 activation, Poly- ADP-ribose polymerase (PARP) degradation, and loss of mitochondrial membrane potential. This process involves a mitochondria-caspase dependent apoptosis pathway. As apoptosis is an important anti-cancer therapeutic target, these results suggest a potential of CWO as a chemotherapeutic agent.

Quantitative determination of polysaccharide in Curcuma wenyujin, a traditional Chinese medicine, by a phenol-sulfuric acid method

Curcuma wenyujin has been widely used as a traditional medicine in China. In this paper a strategy for the quantitative determination of the polysaccharide by a phenol-sulfuric acid method was described. Involved in three factors, 5% phenol volume, H2SO4 volume, and temperature of water bath, we adopted the L9（3）3 orthogonal array design to gain the optimal colorimetric method. 3.0 ml of polysaccharide solution, 1.0 ml, 5% phenol and 7.0 ml H2SO4 were mixed with constant stirring in a glass vessel, and then kept in a water bath at 40 ℃. After cooling to room temperature for 20 min, the absorbance values were recorded by the UV-2501 PC spectrometer at the wavelength range of 485 nm. The polysaccharide content in Curcuma wenyujin were 3.21%, 3.23%, 3.20%, 3.18~/0, 3.22% and 2.38%, respectively. All results showed that this method was adequate, valid and applicable, may be applied to the determination of other bacterial polysaccharide as well.

黄芪莪术不同比例配伍后莪术4种挥发性成分的含量变化研究

目的:探究黄芪莪术不同配伍比例中,莪术挥发油中抗肿瘤活性成分含量的变化。方法:水蒸气蒸馏法对挥发油进行提取,运用气相色谱法,测定单味莪术及黄芪莪术配伍比例为1∶1、2∶1、3∶1、5∶1、1∶2中β-榄香烯、莪术醇、吉马酮、新莪术二酮的含量。采用HP-5气相色谱柱,程序升温法,进样口温度为250℃,FID检测器温度为280℃;内标物质是水杨酸甲酯。结果:β-榄香烯、莪术醇、吉马酮、新莪术二酮4种挥发油分别在0.0030~0.1920 mg·mL^(-1)(r=0.9969)、0.0233~2.9790 mg·mL^(-1)(r=0.9993)、0.0059~0.3760 mg·mL^(-1)(r=0.9994)、0.0175~1.1180 mg·mL^(-1)(r=0.9943)范围呈良好的线性关系,黄芪、莪术以2∶1的比例配伍时,各成分溶出量最高。结论:随着黄芪莪术配伍比例的不同,挥发油中抗肿瘤成分含量有所变化,以2∶1、3∶1的配伍比例为佳,为临床应用和药效学研究提供依据。

不同配方基肥对温郁金产量及其营养元素含量的影响

本试验研究了基肥氮磷钾不同比例配施对温郁金产量与品质的影响,为提高温郁金产量及品质提供科学依据。本研究采用大田试验,设置4个不同的施肥配方,各处理基肥用量均为750 kg·hm^(-2)。结果表明,在氮磷钾总用量相等的前提下,基施配方肥2的温郁金产量最高,与常规施肥比较,温郁金地下部分根茎、块根分别增产11.3%、17.9%。配方肥1和3相较常规施肥也提高了温郁金的产量,但统计上无显著差异。与常规施肥相比,配方肥处理提高了温郁金中氮和磷含量。同时,基施配方肥还可以提高根茎、块根中Ca、Mg及微量元素Zn、B等含量。综上,基肥施用配方肥2既能提高温郁金产量,又能提高根茎、块根中的大量元素及中微量元素含量,具有较好的推广应用价值。

基于网络药理学探究远志-郁金-沉香治疗勃起功能障碍的作用机制

目的:通过“脑-心-肾-精室”轴及网络药理学探究远志-郁金-沉香治疗勃起功能障碍(ED)的作用机制。方法:查询TCMSP、ETCM及相关文献,筛选远志、郁金和沉香的药物成分,使用Swiss Target Prediction预测药物成分的作用靶点;运用GeneCards、OMIM及GisGeNET获取ED的疾病靶点,利用venny 2.1获取交集靶点。使用STRING进行PPI分析并使用Cytoscape构建网络图,利用David数据库进行GO和KEGG分析。使用Cytoscape软件构建“药物-成分-靶点-通路”网络图。利用PubChem、PDB及PyMoL、AutoDock软件进行分子对接。结果:远志-郁金-沉香中的17个有效成分通过多条通路直接作用于65个疾病靶点,从而治疗ED,其中柚皮素、波尔定、鹅掌揪碱、沉香醇、槲皮素等是核心成分,蛋白激酶B(Akt)1、白细胞介素(IL)-6、雄激素受体(AR)、表皮生长因子受体(EGFR)、纤溶酶原激活物抑制剂-1(PAI-1)是至关重要的靶点。基因功能注释分析结果显示,交集基因最可能相关的生物过程主要涉及对缺氧的反应、肽基丝氨酸和蛋白质磷酸化的积极调节、对细胞凋亡的调节等,细胞组分主要涉及膜筏、质膜、细胞质等,分子功能主要涉及蛋白质的结合、酶的结合、一氧化氮合酶活性等。通路富集分析结果提示远志-郁金-沉香主要参与晚期糖基化终末产物-晚期糖基化终末产物受体(AGES-RAGE)、环磷酸鸟苷-蛋白激酶G(cGMP-PKG)等信号通路。分子对接结果提示核心成分与重要靶点间的结合性较好。结论:远志-郁金-沉香主要通过调节AGES-RAGE、cGMP-PKG等信号通路的Akt1、IL-6、AR、EGFR、PAI-1等疾病靶点,干预酶的活性、炎症、氧化应激、血管内皮功能等生物学过程,进而治疗ED。

3种中药配方颗粒全生产链的污染微生物考察

目的:对郁金、桑叶、蝉蜕中药配方颗粒生产全过程污染微生物进行考察,建立数据库,探索中药配方颗粒生产过程微生物关键控制点及控制措施,为企业提供示范性研究,为制定标准、改进生产工艺提供依据,填补质量全过程控制的空白。方法:对3种中药配方颗粒的原材料、中间产品、成品按照《中国药典》2020年版进行分析检测,采用质谱技术对负载微生物进行鉴定,分析研究从药材原料到中药配方颗粒成品的外源性污染微生物的程度及容易污染微生物的种类。结果:3种中药配方颗粒的原材料(中药饮片)微生物污染较严重,原材料的耐热芽孢杆菌即使经过工艺处理后,仍能在中间产品、成品中检出,并造成部分批次需氧菌总数结果超出限度规定;中间产品、成品中还检出来源于人和动物肠道中的屎肠球菌。结论:①加强中药饮片的微生物污染控制,有利于降低终端产品的微生物负载;②3种中药配方颗粒的生产工艺存在芽孢类微生物污染的风险,需优化生产工艺参数来降低微生物负载;③药品生产企业需加强对产品全过程控制,降低微生物污染风险。

基于数据挖掘和网络药理学探讨中药复方治疗抑郁症的用药规律与作用机制

【目的】探讨临床中药复方治疗抑郁症的核心药物及其抗抑郁分子机制。【方法】通过中国知网、万方数据和维普网检索相关文献,筛选并整理临床使用的中药复方。通过分析用药频率和中药关联规则,确定柴胡、白芍和郁金为治疗抑郁症的核心中药。利用TCMSP数据库筛选核心药物的活性成分及靶蛋白,并从TTD、OMIM、DrugBank和GeneCards中获取抑郁症相关靶点。通过STRING和Cytoscape数据库构建蛋白质-蛋白质互作(PPI)网络和“核心药物-活性成分-交集靶点”网络,利用Metascape进行基因本体论(GO)富集和京都基因与基因组百科全书(KEGG)通路富集分析。分子对接分析使用RCSB数据库、AutoDockTools和PyMOL,验证关键活性成分与核心靶标的结合活性。【结果】临床中药复方治疗抑郁症的药物以寒性、甘味、苦味和辛味为主,归经以肝经为首。柴胡、白芍和郁金用药频次最高,且“白芍-柴胡”和“郁金-白芍-柴胡”组合支持度最高。PPI网络中,SLC6A4、AKT1和CHRNA4为关键靶点。GO富集分析表明,核心药物通过化学突触传递等生物过程以及G蛋白偶联受体活性等分子功能,在突触膜等部位发挥作用。KEGG通路富集分析结果显示,核心药物可能通过神经活性配体-受体相互作用、钙信号通路等治疗抑郁症。分子对接证实关键活性成分与核心靶标间有强结合活性。【结论】柴胡、白芍和郁金是临床治疗抑郁症的核心中药,其可通过多成分、多靶点、多通路治疗抑郁症。

黄芪-莪术对Lewis肺癌小鼠NF-κB通路及肿瘤自噬的干预作用研究

目的探讨黄芪-莪术配伍抑制NF-κB通路及诱导Lewis肺癌小鼠肿瘤自噬的作用机制。方法采用腋窝皮下接种肺癌Lewis细胞悬液法建立Lewis肺癌小鼠模型,建模成功后将40只小鼠按随机数字表法分为模型组、中药组、顺铂组和中药+顺铂组,每组各10只。在建模后第3天开始给药,顺铂组给予腹腔注射顺铂(2 mg/kg),1次/3 d,共6次,并每天予灭菌双蒸水灌胃(8.2 g/kg);中药组每日予黄芪-莪术浓煎液灌胃(8.2 g/kg),1次/d,连续18 d,并腹腔注射稀释液[2 mg/(kg·3 d)];中药+顺铂组按照以上给药方法予中药和顺铂;模型组每日予等体积灭菌双蒸水灌胃并腹腔注射稀释液剂量同上。期间观察小鼠毛发、动态及进食、饮水情况,每2 d测量小鼠体质量、每4 d测算小鼠肿瘤体积。在造模20 d后,Western Blot法检测肿瘤组织NF-κBp65、p-NF-κB p65、IκB-α、p-IκB-α、LC3和Beclin-1的蛋白表达水平,ELISA法测定肿瘤组织中TNF-α和IL-1β的含量,并采用透射电镜观察肿瘤细胞超微形态的变化。结果与模型组比较,中药组体质量无明显差异(P>0.05),顺铂组、中药+顺铂组体质量明显较轻,差异有统计学意义(P<0.01),各用药组小鼠肿瘤体积明显减小,差异有统计学意义(P<0.01)、LC3-II/LC3-I及Beclin-1/β-actin值均明显升高,差异有统计学意义(P<0.01)、p-NF-κB p65/NF-κB p65及pIκBα/IκBα值、TNF-α和IL-1β均明显降低,差异有统计学意义(P<0.05);与顺铂组比较,中药+顺铂组肿瘤体积值无明显差异(P>0.05)、Beclin-1/β-actin值明显升高,差异有统计学意义(P<0.01)、p-NF-κB p65/NF-κB p65及pIκBα/IκBα值及TNF-α和IL-1β均明显降低,差异有统计学意义(P<0.05),LC3-II/LC3-I值无明显差异(P>0.05);与中药组比较,中药+顺铂组体质量较轻(P<0.01),肿瘤体积较小,差异有统计学意义(P<0.01),Beclin-1/β-actin值升高,差异有统计学意义(P<0.01),p-NF-κB p65/NF-κB p65、pIκBα/IκBα、TNF-α和IL-1β值均降低,差异有统计学意义(P<0.05),LC3-II/LC3-I值差异无显著性意义(P>0.05)。结论黄芪-莪术配伍可以通过抑制IκB-α的磷酸化减少其降解,达到抑制NF-κB活化,并改善肿瘤炎性微环境,同时上调自噬相关蛋白的表达水平,诱导细胞自噬,进而抑制肿瘤生长。

温郁金根茎腐烂病的病原鉴定

在浙江省瑞安市陶山镇温郁金主产区,部分田块温郁金发生大规模倒伏、地下根茎严重腐烂。为明确引起该病害的病原物,利用LB培养基和TTC(2,3,5-氯化三苯基四氮唑)培养基从温郁金腐烂根茎中分离获得细菌菌株JF1,采用灌根法、芽侵染法和根茎侵染法确定该菌株对温郁金的致病性。通过形态学特征观察、生理生化测定、多基因序列(16S rDNA、rpoB、gyrB)系统发育分析,明确引起田间温郁金根茎腐烂的病原菌为桑肠杆菌(Enterobacter mori)。

基于化学成分结合外观性状的温郁金药材质量分级标准研究

目的:建立温郁金药材的含量测定方法,并与“化学成分-外观性状”相结合,制定温郁金药材的质量分级标准。方法:通过收集59批温郁金药材,测定了外观性状指标,包括长度、直径、50 g块根数等,内在指标包括浸出物、挥发油,并采用高效液相色谱法对莪术二酮和吉马酮的含量进行测定。同时应用SPSS 27.0软件进行聚类分析,采用SIMCA-P 14.1软件进行正交偏最小二乘法判别分析,并对所建立的分级标准进行验证。结果:所建立的含量测定方法,以及基于长度、直径、50 g块根数、浸出物、挥发油、莪术二酮和吉马酮等指标制定的质量分级标准,可将温郁金药材分为3个等级。结论:基于“化学成分-外观性状”相结合的温郁金药材质量分级标准科学合理,为温郁金的质量评价与实现优质优价提供了参考。

黄芪-莪术对C5a介导JAK2/STAT3通路调控Lewis肺癌小鼠Th17/Treg细胞平衡影响的实验研究

目的探讨黄芪-莪术影响C5a介导JAK2/STAT3通路调控肿瘤微环境中Th17/Treg细胞平衡,从而阐明其在此途径抑制肿瘤进展的相关机制。方法40只雄性C57BL/6小鼠按随机数字表法分为空白对照组、模型对照组、中药干预组、阳性对照药(PMX53)组,每组各10只。腋下接种Lewis细胞建立肺癌小鼠模型,于接种后第3天开始,中药干预组按8.2 g/kg剂量给予中药浓煎液灌胃,模型对照组和PMX53组予等容积灭菌双蒸水灌胃,连续14 d;PMX53组分别于第3、6、9、12、15天按1mg/kg剂量给予腹腔注射PMX53,模型对照组和中药干预组同时腹腔注射等容PBS溶液。每2 d测算各组小鼠肿瘤体积、绘制肿瘤生长曲线;于第15天处死,剖取瘤块,ELISA法检测血清C5a、IL6、IL-10、IL-17、TGF-β等因子水平,流式细胞术检测并计算外周血和肿瘤组织中Th17/Treg细胞比例,Western Blot和Real-time PCR法检测肿瘤组织中JAK2/STAT3信号通路相关蛋白及mRNA表达。结果与模型对照组比较,中药干预组和PMX53组肿瘤生长较缓慢,补体C5a、JAK2和STAT3蛋白及mRNA水平、p-JAK2/JAK2、p-STAT3/STAT3比值、Treg占比均降低,SOCS3蛋白及mRNA水平、Th17/Treg比例增高,差异有统计学意义(P<0.05)。结论黄芪-莪术可降低补体C5a进而抑制JAK2/STAT3通路活化,并调节肿瘤微环境中Th17/Treg平衡达到抑制肿瘤生长的作用。

温郁金连作和熏蒸处理土壤微生物种群数量分析

【目的】明确温郁金连作和熏蒸土壤中微生物种群结构,为缓解温郁金连作障碍奠定基础。【方法】选择温郁金连作3年的土壤,在播种前1个月,用棉隆进行土壤熏蒸处理,在温郁金种植7个月后,分别取连作、棉隆熏蒸及未种植温郁金地块等3种土壤,提取土壤总DNA,通过PacBio Sequel微生物3代测序手段,分析土壤真菌及细菌种群数量及多样性。【结果】土壤经熏蒸处理种植的温郁金植株长势好,病害发生少,而连作种植的温郁金植株长势差,死苗较多。细菌群落属水平的分析结果表明,与未种植土壤比较,连作根际土壤中的AD3、TK10和WPS-2的丰度降低。熏蒸土壤中,芽孢杆菌属(Bacillus)和鞘脂单胞菌属(Sphingomonas)等一些具有生防功能的细菌丰度增加。在3种土壤中,有5个真菌门类丰度较高,分别为子囊菌门(Ascomycota)、担子菌门(Basidiomycota)、壶菌门(Chytridiomycota)、被孢菌门(Mortierellomycota)以及未分类真菌门(unclassified_k_Fungi)。其中子囊菌门真菌在未种植土壤中丰度最低,连作使子囊菌门真菌的丰度显著升高、担子菌门真菌的丰度显著降低。大量有益的真菌属,如毛壳菌属(Chaetomium)、青霉属(Penicillium)、曲霉属(Aspergillus)、漆斑菌属(Myrothecium)、梭孢壳霉属(Thielavia)、腐质霉属(Humicola)、Rhizophlyctis、Microdochium、Ramicandelaber、Albifimbria、Curvularia、巨孢囊霉属(Gigaspora)、黑孢菌属(Nigrospora)、Neocosmospora、Talaromyces、Roussoella、Rhytidhysteron、Boothiomyces、Apiotrichum、Ochroconis、Saitozyma、丝孢酵母属(Trichosporon)、茎点霉属(Phoma)等在熏蒸土壤中的丰度明显高于连作土壤。而在连作土壤中则表现为较多的致病微生物的累积。【结论】温郁金连作可使土壤有害微生物的积累,造成温郁金连作障碍,施用棉隆熏蒸处理土壤后,可减少土壤中病原菌的数量,增加有益微生物种群数量,削减温郁金的连作障碍。

网络药理学结合分子对接技术探讨黄芪-莪术促进肺癌肿瘤血管正常化的潜在机制

目的通过网络药理学联合分子对接技术探讨黄芪-莪术促进肺癌肿瘤血管正常化的潜在作用机制,为进一步研究寻找方向。方法利用公共数据库(TCMSP)检索并获取黄芪-莪术活性成分及相关靶点,通过基因卡、OMIM、TTD、药物GKB数据库和药物库数据,发现肺癌血管正常化的相关靶点,Met细胞景观插件ClueGO平台用于基因本体(GO)富集分析和京都基因组百科全书(KEGG)通路富集分析,最后利用AutoDock及Vina软件进行分子对接打分,Pymol软件进行对接结果的可视化展示。结果共筛选出486个黄芪-莪术生物活性成分和184个黄芪-莪术与肺癌肿瘤血管正常化相关的靶点;根据生物信息学分析,槲皮素、异鼠李素、山奈酚、7-O-甲基异脲醇、芒柄花黄素、毛芯异黄酮可能是黄芪-莪术调控肺癌肿瘤血管正常化的潜在活性成分,而RB1、MAPK1、EGFR、ESR1、AKT1、HIF1A、TP53、STAT1和CCND1是潜在的起效靶点;根据富集和分子对接结果显示异鼠李素与STAT1之间具有较高的结合亲和力,JAK2/STAT1通路可能是黄芪-莪术调控肺癌血管正常化的主要作用通路。结论黄芪-莪术促进肺癌肿瘤血管正常化作用的潜在机制可能是IFN-γ-JAK2/STAT1通路。

基于WGCNA筛选胃癌相关自然杀伤细胞基因及潜在中药预测

目的:通过加权基因共表达网络分析(WGCNA)筛选胃癌相关自然杀伤细胞基因,探讨自然杀伤细胞相关基因与胃癌预后相关性,预测潜在治疗的中药。方法:运用WGCNA分别构建TCGA-STAD数据集和GSE84437数据集胃癌自然杀伤细胞相关基因共表达网络,获得自然杀伤细胞相关基因。通过单因素Cox回归分析获得与预后相关基因,对预后相关基因进行生存分析,获得与生存相关的基因,以GSE84433作为验证集。运用CIBERSORT反卷积算法计算生存相关基因在22种免疫细胞中的浸润情况。将预后相关的基因映射到Coremine Medical数据库,获得潜在中药,并对筛选中药进行四气、五味、归经及功效的频数统计。结果:通过WGCNA及差异分析,筛选出98个与自然杀伤细胞相关基因,单因素Cox分析共获得22个与预后相关的基因,通过生存分析,发现5个基因与生存显著相关,且与大部分免疫细胞存在相关性,并在GSE84433数据集得到验证。将预后相关基因映射到Coremine Medical数据库,获得潜在治疗中药186味,其中姜黄、郁金等10味中药出现频数较高,药性以温、寒为主,药味以苦、辛、甘为主,归经以肝经、肺经、胃经、脾经为主,功效以补虚药、清热药为主。结论:IL32、IRF8、INFG、SNX10、BATF2等5个胃癌相关自然杀伤细胞基因与胃癌预后相关,姜黄、郁金等中药可能通过提高自然杀伤细胞的活性和数量改善胃癌的预后。本研究揭示中药可能通过改变肿瘤免疫微环境改善患者生存预后,为后续中药研究提供思路。

基于网络药理学和分子对接探讨姜黄、郁金药对治疗肝癌的作用机制

目的:基于网络药理学和分子对接方法探讨姜黄、郁金药对治疗肝癌的潜在靶点和作用机制。方法:通过公共数据库筛选姜黄、郁金药对的活性成分和作用靶点及肝癌的靶点,绘制药物活性成分的分子靶点和疾病靶点的交集,构建药对-疾病-有效成分-靶点的中药复方调控网络图及蛋白-蛋白互作网络图,进行GO富集分析和KEGG信号通路富集分析,通过分子对接对有效成分和关键靶点进行验证。基于TIMER数据库分析关键靶点与免疫细胞浸润的关系,通过GEPIA数据库分析关键靶点在肿瘤组织中的表达情况及对患者临床预后的影响。结果:最终筛选出姜黄、郁金药对有效成分6个,共同靶点70个,核心靶点柚皮素与MAPK3、CASP3、AKT1、CAT、ESR1和PTGS2有较强的亲和力。作用机制涉及多条通路,核心靶点与多个免疫细胞浸润丰度相关,其中MAPK3在肿瘤组织中的表达高于癌旁组织,ESR1则相反。生存曲线分析中MAPK3表达水平高者,预后相对较差;ESR1表达水平高者,预后相对较好。MAPK3为癌基因,ESR1为抑癌基因,对肝癌的临床预后具有潜在价值。结论:姜黄、郁金药对可通过诱导肿瘤细胞凋亡、抑制肿瘤血管生成、调节信号通路减少肿瘤免疫逃逸等过程发挥多成分、多靶点、多途径协同抗肿瘤作用,为进一步药理研究提供了新思路和线索。

温郁金地上部分的化学成分(英文)

目的:研究中药莪术来源植物之一温郁金地上部分的化学成分。方法:利用反复硅胶色谱柱进行分离纯化,通过理化性质和波谱数据分析鉴定化合物结构。结果:分离并鉴定出6种化合物:codonolactone(1),vole-neol(2),octacosanoic ac id(3),β-谷甾醇(4),m angdesisterol(5)和胡萝卜苷(6)。结论:两个倍半萜化合物1,2和一个甾醇5为首次从该植物中分得。