RAPD and RFLP analyses of double haploid lines which derived from hybridization between hexaploid wheat (Triticum aestivum L. 2n=42) and eastern gamagrass (Tripsacum dactyloides L. 2n=4x=72) are reported. Two of the 3...RAPD and RFLP analyses of double haploid lines which derived from hybridization between hexaploid wheat (Triticum aestivum L. 2n=42) and eastern gamagrass (Tripsacum dactyloides L. 2n=4x=72) are reported. Two of the 340 Operon primers have been screened, which stably amplified Tripsacum dactyloides (male parent) specific bands in the double haploid lines. These results confirm the fact that Tripsacum dactyloides DNA has been integrated into wheat genome by sexual hybridization at molecular level. This idea has been further testified by RFLP analysis. Application and potentials of transferring Tripsacum dactyloides DNA into wheat genome by sexual hybridization in wheat breeding are discussed.展开更多
Tripsacum dactyloides (L.) L., commonly known as eastern gamagrass, is useful for grazing, stored forage, soil amelioration and conservation, and as a biofuel feedstock. Our goal was to calculate accumulated growing d...Tripsacum dactyloides (L.) L., commonly known as eastern gamagrass, is useful for grazing, stored forage, soil amelioration and conservation, and as a biofuel feedstock. Our goal was to calculate accumulated growing degree days (GDD) from existing datasets collected for eastern gamagrass forage production experiments in northwestern Oklahoma, and discuss the use of GDD, instead of calendar harvest dates, in the production of eastern gamagrass forage. Growing degree days were calculated from 1 January each year using the “optimum day method”. For 10 harvest years, the first eastern gamagrass harvest required 690 ± 26 cumulative GDD. Based on long-term weather data from Woodward, Oklahoma, this would place the first harvest on or near 1 June. The second harvest required 635 ± 27 cumulative GDD which would place the second harvest on or near 15 July and the third harvest required 690 ± 23 cumulative GDD placing it on or near 30 August. Each of the 30 harvest required an average of 670 ± 15 cumulative GDD. Using GDD to predict harvest events is a useful tool that forage producer can use in the production of eastern gamagrass forage in the USA and possibly elsewhere.展开更多
文摘RAPD and RFLP analyses of double haploid lines which derived from hybridization between hexaploid wheat (Triticum aestivum L. 2n=42) and eastern gamagrass (Tripsacum dactyloides L. 2n=4x=72) are reported. Two of the 340 Operon primers have been screened, which stably amplified Tripsacum dactyloides (male parent) specific bands in the double haploid lines. These results confirm the fact that Tripsacum dactyloides DNA has been integrated into wheat genome by sexual hybridization at molecular level. This idea has been further testified by RFLP analysis. Application and potentials of transferring Tripsacum dactyloides DNA into wheat genome by sexual hybridization in wheat breeding are discussed.
文摘Tripsacum dactyloides (L.) L., commonly known as eastern gamagrass, is useful for grazing, stored forage, soil amelioration and conservation, and as a biofuel feedstock. Our goal was to calculate accumulated growing degree days (GDD) from existing datasets collected for eastern gamagrass forage production experiments in northwestern Oklahoma, and discuss the use of GDD, instead of calendar harvest dates, in the production of eastern gamagrass forage. Growing degree days were calculated from 1 January each year using the “optimum day method”. For 10 harvest years, the first eastern gamagrass harvest required 690 ± 26 cumulative GDD. Based on long-term weather data from Woodward, Oklahoma, this would place the first harvest on or near 1 June. The second harvest required 635 ± 27 cumulative GDD which would place the second harvest on or near 15 July and the third harvest required 690 ± 23 cumulative GDD placing it on or near 30 August. Each of the 30 harvest required an average of 670 ± 15 cumulative GDD. Using GDD to predict harvest events is a useful tool that forage producer can use in the production of eastern gamagrass forage in the USA and possibly elsewhere.
