Wheat dwarf bunt, caused by Tilletia controversa Kühn （TCK）, is an important quarantine wheat disease throughout the world. Based on published research results of the biology and the epidemiology of the disease...Wheat dwarf bunt, caused by Tilletia controversa Kühn （TCK）, is an important quarantine wheat disease throughout the world. Based on published research results of the biology and the epidemiology of the disease, the main factors including temperature, humidity, snow cover, and their parameters relating to teliospore germination, infection and epidemics of TCK were determined. The geophytopathological models for the risk analysis of wheat dwarf bunt establishment were modified. Fifty-year meteorologic data from about 500 weather stations in China were used to calculate the probabilities of TCK establishment in different geographic sites with the models. The map that displays the establishment risk of TCK in winter wheat growing regions in China was generated by using geographical information system （GIS）. The zones showing high, moderate, low, and very low, including no risk, of TCK establishment accounted for 27.33, 27.69, 38.12, and 6.86% of total winter wheat growing areas in China, respectively. These results will provide useful information to formulate quarantine regulations and wheat importation policy in China.展开更多
基金supported by the National Basic Research and Development Program of China (2009CB119200,2002CB111405)the project from Ministry of Agriculture of China
文摘Wheat dwarf bunt, caused by Tilletia controversa Kühn （TCK）, is an important quarantine wheat disease throughout the world. Based on published research results of the biology and the epidemiology of the disease, the main factors including temperature, humidity, snow cover, and their parameters relating to teliospore germination, infection and epidemics of TCK were determined. The geophytopathological models for the risk analysis of wheat dwarf bunt establishment were modified. Fifty-year meteorologic data from about 500 weather stations in China were used to calculate the probabilities of TCK establishment in different geographic sites with the models. The map that displays the establishment risk of TCK in winter wheat growing regions in China was generated by using geographical information system （GIS）. The zones showing high, moderate, low, and very low, including no risk, of TCK establishment accounted for 27.33, 27.69, 38.12, and 6.86% of total winter wheat growing areas in China, respectively. These results will provide useful information to formulate quarantine regulations and wheat importation policy in China.
基金National Basic Research and Development Program of China("973"Program:2002CB111406)National Basic Researchand Development Program of China(2002CB111406)Program for Changjiang Scholars and Innovative ResearchTeam in University(IRT1042)
文摘小麦矮腥黑穗病菌(Tilletia controversa Kühn,简称TCK)是小麦上的一种重要检疫性真菌。本研究利用内部简单重复序列(Inter-simple sequence repeat,ISSR)技术研究TCK及其近缘种的DNA多态性,开发了一种可靠而简单的方法用于TCK的分子鉴定。用ISSR引物P4从TCK中扩增出一条1 113 bp的特异性条带,据此设计了一对特异性引物TCKF/TCKR,在12个TCK菌株中均能扩增得到一条882 bp的特异性条带,而其他近缘种包括小麦网腥黑穗病菌(T.caries)和小麦光腥黑穗病菌(T.foetida)及相关黑粉菌的14个菌株均无扩增条带。用该特异性引物检测TCK的下限为25μL反应体系中可检测到1 ng DNA模板。本研究开发的种特异性引物,可将TCK与其形态上相似的近缘种尤其是小麦网腥黑穗病菌准确区分开,本研究基于ISSR标记建立的小麦矮腥黑穗病菌的分子鉴定方法为腥黑粉菌的检疫提供了一种便捷的方法,是对现有分子鉴定方法的一个补充。
