Monocot high expression vector pBARUNP1, harboring rabbit defensin(NP 1) gene and selective bar gene for resistance to the herbicide Basta, were constructed and then transferred into immature embryos of wheat (“Bobw...Monocot high expression vector pBARUNP1, harboring rabbit defensin(NP 1) gene and selective bar gene for resistance to the herbicide Basta, were constructed and then transferred into immature embryos of wheat (“Bobwhite” and “Zhong 60634”)via particle bombardment. Southern and RNA dot blots showed the stable integration and transcription of foreign NP 1 gene in the wheat genome. Furthermore, in vitro microbicidal activity assay indicated the proper translation of defensin. Crude protein extraction of transgenic plants exhibited to some extent cytotoxic to several pathogens including G. saubinetii, B. subtilis, E.coli, and A. tumefaciens.展开更多
In China, the purity of maize hybrid strain is discomforting to the development of seed industrialization. Finding a new method for reproduction of maize hybrid strain is necessary. In this study, using particle bomba...In China, the purity of maize hybrid strain is discomforting to the development of seed industrialization. Finding a new method for reproduction of maize hybrid strain is necessary. In this study, using particle bombardment, barstar gene was transferred into maize inbred line 18-599 （White）, which is an antiviral and high quality maize inbred line. By molecular detection of the anther of transgenic maize, two plants transferred with barstar gene were gained in this study, which are two restorer lines. The two plants showed normal male spike, and lively microspores. But the capacity of the two restorer lines should be studied in the future. The aim of this study is to find a new method of reproduction of maize hybrid strain using engineering restorer lines and engineering sterility lines by gene engineering technology.展开更多
Over the last two decades, transgenic plants have moved from being solely laboratory vehicles for basic research work to providing new varieties grown on large areas throughout the world. A number of
β-glucuronidase (GUS) gene (carried by pBI121 plasmid) has been introduced into hexaploid wheat c. v. Xiaoshan No. 3 with the pollen-tube pathway method. Five transgenic plants are selected from 106 T0 plants. All of...β-glucuronidase (GUS) gene (carried by pBI121 plasmid) has been introduced into hexaploid wheat c. v. Xiaoshan No. 3 with the pollen-tube pathway method. Five transgenic plants are selected from 106 T0 plants. All of these plants are identified by the Southern hybridization, and the GUS activity can be detected by using fluorogenic assay and histochemical stain.展开更多
文摘Monocot high expression vector pBARUNP1, harboring rabbit defensin(NP 1) gene and selective bar gene for resistance to the herbicide Basta, were constructed and then transferred into immature embryos of wheat (“Bobwhite” and “Zhong 60634”)via particle bombardment. Southern and RNA dot blots showed the stable integration and transcription of foreign NP 1 gene in the wheat genome. Furthermore, in vitro microbicidal activity assay indicated the proper translation of defensin. Crude protein extraction of transgenic plants exhibited to some extent cytotoxic to several pathogens including G. saubinetii, B. subtilis, E.coli, and A. tumefaciens.
文摘In China, the purity of maize hybrid strain is discomforting to the development of seed industrialization. Finding a new method for reproduction of maize hybrid strain is necessary. In this study, using particle bombardment, barstar gene was transferred into maize inbred line 18-599 （White）, which is an antiviral and high quality maize inbred line. By molecular detection of the anther of transgenic maize, two plants transferred with barstar gene were gained in this study, which are two restorer lines. The two plants showed normal male spike, and lively microspores. But the capacity of the two restorer lines should be studied in the future. The aim of this study is to find a new method of reproduction of maize hybrid strain using engineering restorer lines and engineering sterility lines by gene engineering technology.
文摘Over the last two decades, transgenic plants have moved from being solely laboratory vehicles for basic research work to providing new varieties grown on large areas throughout the world. A number of
文摘β-glucuronidase (GUS) gene (carried by pBI121 plasmid) has been introduced into hexaploid wheat c. v. Xiaoshan No. 3 with the pollen-tube pathway method. Five transgenic plants are selected from 106 T0 plants. All of these plants are identified by the Southern hybridization, and the GUS activity can be detected by using fluorogenic assay and histochemical stain.
