Members of the transforming growth factor-beta superfamily, growth differentiation factor 9 （GDF9） and bone morphogenetic protein 15 （BMP 15）, have crucial roles in fecundity of sheep. Our previous investigation c...Members of the transforming growth factor-beta superfamily, growth differentiation factor 9 （GDF9） and bone morphogenetic protein 15 （BMP 15）, have crucial roles in fecundity of sheep. Our previous investigation confirmed that the fecundity mutations of sheep presented in highly prolific White goat individuals of Guizhou province. To illuminate other polymorphisms in Bmpl5 and Gdfl） genes and the relationship of these mutations with function, we cloned and characterized the coding region of Bmp15 and Gdfl）. Molecular models of BMP15 and GDF9 mature peptide of White goat were constructed based on the homology of human BMP7 experimental tertiary structure. Two exons encoded prepropeptide of 394 amino acids in BMPI5 and 453 residues in GDF9, respectively. Apart from the FecXs mutation （S99I） in BMP15 and V791 mutation in GDF9 confirmed in White goat previously, other seven and three polymorphism sites were detected from BMP15 and GDF9 mature peptides, respectively. S32G, N66H, S99I/P99I and G107R in BMP15 could be important for the binding of dimer to receptors. Changes of P78Q and V79I in GDF9 might affect the binding of dimer to receptor type t. Comparing the length of BMP 15 and GDF9 prepropeptide in vertebrates, an increase in length of BMP 15 presented along with the protein evolution from fish to mammal and the divergence of the N-terminus residues in matured BMP15 peptide might contribute to the sensitive control on the fertility of animal species with low ovulation rate. These findings gave a valuable explanation for the correlation of mutations in Bmpl5 and Gdfl） genes with the control on fecundity of White goat and supported the notion that they were the pivotal factors in female fertility of White goat in Guizhou province.展开更多
In this research,changes of testosterone hormone in the blood serum of 9 male（3 of age 2 and 6 of age 4） White goats have been studied for a duration of one year.Analyses performed show that the general monthly aver...In this research,changes of testosterone hormone in the blood serum of 9 male（3 of age 2 and 6 of age 4） White goats have been studied for a duration of one year.Analyses performed show that the general monthly averages of the testosterone hormone of the male White goats in the period of 12 months starting in January were（2.11±.73）,（2.22±.52）,（2.44±.59）,（2.10±.41）,（2.35±.00）,（3.42±.48）,（4.77±.32）,（5.61±.40）,（7.91±.24）,（10.84±.59）,（7.82±.60）,and（2.63±.61） ng mL-1,respectively（P0.05）.The effect of the age factor on the testosterone production in White goats（age 2 and 4） was generally significant（P 0.05） for differences among months in each age group,but not significant for differences between age groups in each month.The testosterone hormone levels of male White goats encountered significant changes（P0.05） with respect to the season.展开更多
该研究旨在通过全基因组关联分析挖掘内蒙古阿尔巴斯白绒山羊早期生长性状的重要变异位点及候选基因,为该品种的遗传改良提供重要的分子标记。基于山羊70 k SNP芯片对2299只内蒙古阿尔巴斯白绒山羊个体进行基因分型,对表型、环境效应以...该研究旨在通过全基因组关联分析挖掘内蒙古阿尔巴斯白绒山羊早期生长性状的重要变异位点及候选基因,为该品种的遗传改良提供重要的分子标记。基于山羊70 k SNP芯片对2299只内蒙古阿尔巴斯白绒山羊个体进行基因分型,对表型、环境效应以及个体基因型建立混合线性模型开展GWAS研究。结果显示,在全基因组上共检测出24个与绒山羊早期生长性状显著相关的SNP位点,定位到31个候选基因,其中与初生重、断奶重、断奶前日增重和周岁重显著相关的候选基因分别有6、 11、 12和2个。GO功能和KEGG通路分析结果表明,这些候选基因中最显著的生物学过程为分子功能的钙依赖性磷脂酶A2活性、通路为α-亚麻酸代谢通路和亚油酸代谢通路。可见,通过全基因组关联分析可筛选到阿尔巴斯白绒山羊早期生长性状的重要候选基因,根据筛选的重要基因可有目标的对阿尔巴斯白绒山羊早期生长性状进行选育。展开更多
基金the National Natural Science Foundation of China (30560104)~~
文摘Members of the transforming growth factor-beta superfamily, growth differentiation factor 9 （GDF9） and bone morphogenetic protein 15 （BMP 15）, have crucial roles in fecundity of sheep. Our previous investigation confirmed that the fecundity mutations of sheep presented in highly prolific White goat individuals of Guizhou province. To illuminate other polymorphisms in Bmpl5 and Gdfl） genes and the relationship of these mutations with function, we cloned and characterized the coding region of Bmp15 and Gdfl）. Molecular models of BMP15 and GDF9 mature peptide of White goat were constructed based on the homology of human BMP7 experimental tertiary structure. Two exons encoded prepropeptide of 394 amino acids in BMPI5 and 453 residues in GDF9, respectively. Apart from the FecXs mutation （S99I） in BMP15 and V791 mutation in GDF9 confirmed in White goat previously, other seven and three polymorphism sites were detected from BMP15 and GDF9 mature peptides, respectively. S32G, N66H, S99I/P99I and G107R in BMP15 could be important for the binding of dimer to receptors. Changes of P78Q and V79I in GDF9 might affect the binding of dimer to receptor type t. Comparing the length of BMP 15 and GDF9 prepropeptide in vertebrates, an increase in length of BMP 15 presented along with the protein evolution from fish to mammal and the divergence of the N-terminus residues in matured BMP15 peptide might contribute to the sensitive control on the fertility of animal species with low ovulation rate. These findings gave a valuable explanation for the correlation of mutations in Bmpl5 and Gdfl） genes with the control on fecundity of White goat and supported the notion that they were the pivotal factors in female fertility of White goat in Guizhou province.
基金funded by Ankara University Scientific Research Projects Directorate,Turkey (20060711099)
文摘In this research,changes of testosterone hormone in the blood serum of 9 male（3 of age 2 and 6 of age 4） White goats have been studied for a duration of one year.Analyses performed show that the general monthly averages of the testosterone hormone of the male White goats in the period of 12 months starting in January were（2.11±.73）,（2.22±.52）,（2.44±.59）,（2.10±.41）,（2.35±.00）,（3.42±.48）,（4.77±.32）,（5.61±.40）,（7.91±.24）,（10.84±.59）,（7.82±.60）,and（2.63±.61） ng mL-1,respectively（P0.05）.The effect of the age factor on the testosterone production in White goats（age 2 and 4） was generally significant（P 0.05） for differences among months in each age group,but not significant for differences between age groups in each month.The testosterone hormone levels of male White goats encountered significant changes（P0.05） with respect to the season.
文摘该研究旨在通过全基因组关联分析挖掘内蒙古阿尔巴斯白绒山羊早期生长性状的重要变异位点及候选基因,为该品种的遗传改良提供重要的分子标记。基于山羊70 k SNP芯片对2299只内蒙古阿尔巴斯白绒山羊个体进行基因分型,对表型、环境效应以及个体基因型建立混合线性模型开展GWAS研究。结果显示,在全基因组上共检测出24个与绒山羊早期生长性状显著相关的SNP位点,定位到31个候选基因,其中与初生重、断奶重、断奶前日增重和周岁重显著相关的候选基因分别有6、 11、 12和2个。GO功能和KEGG通路分析结果表明,这些候选基因中最显著的生物学过程为分子功能的钙依赖性磷脂酶A2活性、通路为α-亚麻酸代谢通路和亚油酸代谢通路。可见,通过全基因组关联分析可筛选到阿尔巴斯白绒山羊早期生长性状的重要候选基因,根据筛选的重要基因可有目标的对阿尔巴斯白绒山羊早期生长性状进行选育。
