The Scarlet Letter makes the American writer Nathaniel Hawthorne known all around the world, while The White Snake is one of the most famous legends in China. This paper compares the two miserable heroines and reveals...The Scarlet Letter makes the American writer Nathaniel Hawthorne known all around the world, while The White Snake is one of the most famous legends in China. This paper compares the two miserable heroines and reveals all the religions serve to the ruling power.展开更多
The evolution of southwest Montana’s Big Hole and Beaverhead River drainage basins is determined from topographic map evidence related to mountain passes crossing what are today high altitude drainage divides includi...The evolution of southwest Montana’s Big Hole and Beaverhead River drainage basins is determined from topographic map evidence related to mountain passes crossing what are today high altitude drainage divides including North America’s east-west Continental Divide. Map evidence, such as orientations of valleys leading away from mountain passes (and saddles) and barbed tributaries found along the downstream drainage routes, is used to reconstruct flow directions of streams and rivers that once crossed the present-day high mountain divides. Large south-oriented anastomosing complexes of diverging and converging channels are interpreted to have eroded what are today closely spaced passes and saddles now notched into high mountain ridges. Water in those south-oriented channels is interpreted to have flowed across emerging mountains and subsiding basins. Headward erosion of deeper southeast-oriented valleys, assisted by crustal warping, concentrated south-oriented water in fewer and deeper valleys as the water flowed from southwest Montana into what are today Idaho and the Snake River drainage basin. Headward erosion of the Big Hole River valley between the emerging Anaconda and Pioneer Mountains, also assisted by crustal warping, reversed all Big Hole Basin drainage so as to create the north-, east-, and south-oriented Big Hole River drainage route. A final and even more major reversal of flow in the present-day north-oriented Montana Missouri River valley, with the assistance of additional crustal warping, next ended all remaining flow to Idaho and the Snake River drainage basin and reversed and captured all drainage in the present-day north-oriented Big Hole, Beaverhead, and Red Rock River drainage basins. The observed map evidence indicates that prior to the final flow reversal events, large volumes of south-oriented water flowed across southwest Montana’s Big Hole and Beaverhead River drainage basins.展开更多
The many-banded krait,Bungarus multicinctus,has been recorded as the animal resource of JinQianBaiHuaShe in the Chinese Pharmacopoeia.Characterization of its venoms classified chief phyla of modern animal neurotoxins....The many-banded krait,Bungarus multicinctus,has been recorded as the animal resource of JinQianBaiHuaShe in the Chinese Pharmacopoeia.Characterization of its venoms classified chief phyla of modern animal neurotoxins.However,the evolutionary origin and diversification of its neurotoxins as well as biosynthesis of its active compounds remain largely unknown due to the lack of its high-quality genome.Here,we present the 1.58 Gbp genome of B.multicinctus assembled into 18 chromosomes with contig/scaffold N50 of 7.53 Mbp/149.8 Mbp.Major bungarotoxin-coding genes were clustered within genome by family and found to be associated with ancient local duplications.The truncation of glycosylphosphatidylinositol anchor in the 3'-terminal of a LY6E paralog released modern three-finger toxins(3FTxs)from membrane tethering before the Colubroidea divergence.Subsequent expansion and mutations diversified and recruited these 3FTxs.After the cobra/krait divergence,the modern unit-B ofβ-bungarotoxin emerged with an extra cysteine residue.A subsequent point substitution in unit-A enabled theβ-bungarotoxin covalent linkage.The B.multicinctus gene expression,chromatin topological organization,and histone modification characteristics were featured by transcriptome,proteome,chromatin conformation capture sequencing,and ChIP-seq.The results highlighted that venom production was under a sophisticated regulation.Our findings provide new insights into snake neurotoxin research,meanwhile will facilitate antivenom development,toxin-driven drug discovery and the quality control of JinQianBaiHuaShe.展开更多
文摘The Scarlet Letter makes the American writer Nathaniel Hawthorne known all around the world, while The White Snake is one of the most famous legends in China. This paper compares the two miserable heroines and reveals all the religions serve to the ruling power.
文摘The evolution of southwest Montana’s Big Hole and Beaverhead River drainage basins is determined from topographic map evidence related to mountain passes crossing what are today high altitude drainage divides including North America’s east-west Continental Divide. Map evidence, such as orientations of valleys leading away from mountain passes (and saddles) and barbed tributaries found along the downstream drainage routes, is used to reconstruct flow directions of streams and rivers that once crossed the present-day high mountain divides. Large south-oriented anastomosing complexes of diverging and converging channels are interpreted to have eroded what are today closely spaced passes and saddles now notched into high mountain ridges. Water in those south-oriented channels is interpreted to have flowed across emerging mountains and subsiding basins. Headward erosion of deeper southeast-oriented valleys, assisted by crustal warping, concentrated south-oriented water in fewer and deeper valleys as the water flowed from southwest Montana into what are today Idaho and the Snake River drainage basin. Headward erosion of the Big Hole River valley between the emerging Anaconda and Pioneer Mountains, also assisted by crustal warping, reversed all Big Hole Basin drainage so as to create the north-, east-, and south-oriented Big Hole River drainage route. A final and even more major reversal of flow in the present-day north-oriented Montana Missouri River valley, with the assistance of additional crustal warping, next ended all remaining flow to Idaho and the Snake River drainage basin and reversed and captured all drainage in the present-day north-oriented Big Hole, Beaverhead, and Red Rock River drainage basins. The observed map evidence indicates that prior to the final flow reversal events, large volumes of south-oriented water flowed across southwest Montana’s Big Hole and Beaverhead River drainage basins.
基金the Fundamental Research Funds for the Central Public Welfare Research Institutes(ZZ13-YQ-047,ZXKT22006,China)quality standard system construction for the whole industry chain of Chinese medicine from Guangdong Provincial Drug Administration of China(002009/2019KT1261/2020ZDB25)+2 种基金the National Major Science and Technology Projects(2019ZX09201005,China)the Open Research Fund of Chengdu University of Traditional Chinese Medicino state Key Laboratory scluthwestern Chinese Medicine Resources(2022ZYXK2011006,China)the National Key R&D Program of China(2019YFC1711100)。
文摘The many-banded krait,Bungarus multicinctus,has been recorded as the animal resource of JinQianBaiHuaShe in the Chinese Pharmacopoeia.Characterization of its venoms classified chief phyla of modern animal neurotoxins.However,the evolutionary origin and diversification of its neurotoxins as well as biosynthesis of its active compounds remain largely unknown due to the lack of its high-quality genome.Here,we present the 1.58 Gbp genome of B.multicinctus assembled into 18 chromosomes with contig/scaffold N50 of 7.53 Mbp/149.8 Mbp.Major bungarotoxin-coding genes were clustered within genome by family and found to be associated with ancient local duplications.The truncation of glycosylphosphatidylinositol anchor in the 3'-terminal of a LY6E paralog released modern three-finger toxins(3FTxs)from membrane tethering before the Colubroidea divergence.Subsequent expansion and mutations diversified and recruited these 3FTxs.After the cobra/krait divergence,the modern unit-B ofβ-bungarotoxin emerged with an extra cysteine residue.A subsequent point substitution in unit-A enabled theβ-bungarotoxin covalent linkage.The B.multicinctus gene expression,chromatin topological organization,and histone modification characteristics were featured by transcriptome,proteome,chromatin conformation capture sequencing,and ChIP-seq.The results highlighted that venom production was under a sophisticated regulation.Our findings provide new insights into snake neurotoxin research,meanwhile will facilitate antivenom development,toxin-driven drug discovery and the quality control of JinQianBaiHuaShe.
