[Objective] The paper aimed to analyze the polymorphism of the growth hormone receptor （GHR） in improved hybrid yellow cattle group from Liupan Mountain area in Ningxia Autonomous Region,so as to provide technologic...[Objective] The paper aimed to analyze the polymorphism of the growth hormone receptor （GHR） in improved hybrid yellow cattle group from Liupan Mountain area in Ningxia Autonomous Region,so as to provide technological basis for hybrid improvement. [Method] Polymerase chain reaction-restriction fragment length polymorphisms （PCR-RFLP） technology was carried out to examine polymorphisms of GHR gene of 70 individuals. [Result] The target fragment of 338 bp was amplified. The PCR product digested by restriction enzyme Alu I showed polymorphisms. The frequencies of the two genotypes （AA,BB） were 75.71% （53 individuals） and 24.29% （17 individuals）,respectively. [Conclusion] Two genotypes of GHR gene were detected in improved hybrid yellow cattle groups from Liupan Mountain area in Ningxia.展开更多
[Objective] The paper aimed to study the improved effect of local beef cattle with Fleckvieh cattle and provide theoretical basic data for local cattle industry in central plain agricultural area in China. [Method] Wi...[Objective] The paper aimed to study the improved effect of local beef cattle with Fleckvieh cattle and provide theoretical basic data for local cattle industry in central plain agricultural area in China. [Method] With 500 local beef cattle as female parents and Fleckvieh cattle as male parents,hybridization improvement was conducted via artificial insemination. The growth performance,slaughter performance,milk performance and milk components of F1 and F2 hybrids were measured. [Result] The birth body weights of F2 were significantly higher than those of local beef cow,but there was no remarkable difference between F1 and local beef cow or F1 and F2. The growth rates of F1 and F2 at different stages were higher than those of local beef cattle. The slaughter performance,such as carcass weight( P < 0. 05),dressing percentage,net meat rate( P < 0. 05),marbling score of F1 and F2 were higher than those of local beef cow. Milk production performance,such as actual milk yield,305 d corrected milk yield and 4% standard milk yield of F2 were signally higher than those of F1 and local beef cattle( P< 0. 05),and F1 was markedly higher than local beef cattle( P < 0. 05). For milk composition,although milk fat percentage,milk protein rate,lactose rate and total solids( TS) of F1 and F2 were slightly lowered compared with local beef cattle at varying degrees,they were still at high levels compared with Holstein cows.[Conclusion]Fleckvieh cattle,as a male parent,can significantly improved growth performance,slaughter performance and milk performance of offsprings. It would also increases the economic efficiency of local beef cattle by higher quality and price,as well as changing production model from beef to dual purpose of beef and milk.展开更多
In the improvement process of local yellow cattle with frozen semen of beef cattle, the cows were grouped according to different cross combinations. Then, the conception rate, dystocia rate and reproductive survival r...In the improvement process of local yellow cattle with frozen semen of beef cattle, the cows were grouped according to different cross combinations. Then, the conception rate, dystocia rate and reproductive survival rate of the cows in each group, as well as the body size and body weight of new-born calves, were ana- lyzed. The results showed that no significant differences were found in conception rate among different groups （P〉0.05）; there was no significant difference in dystocia rate between the A and C groups （P〉0.05）, but the dystocia rate in the B group was significantly different from those in the A and C groups （P〈0.05）. There were positive correlations between calf forehead width, chest circumference and the dys- tocia rate.展开更多
基金Supported by Ningxia Science and Technology Program(5183003)Action Project for Scientific and Technological Personal to Service Enterprise (2009GJG30036)~~
文摘[Objective] The paper aimed to analyze the polymorphism of the growth hormone receptor （GHR） in improved hybrid yellow cattle group from Liupan Mountain area in Ningxia Autonomous Region,so as to provide technological basis for hybrid improvement. [Method] Polymerase chain reaction-restriction fragment length polymorphisms （PCR-RFLP） technology was carried out to examine polymorphisms of GHR gene of 70 individuals. [Result] The target fragment of 338 bp was amplified. The PCR product digested by restriction enzyme Alu I showed polymorphisms. The frequencies of the two genotypes （AA,BB） were 75.71% （53 individuals） and 24.29% （17 individuals）,respectively. [Conclusion] Two genotypes of GHR gene were detected in improved hybrid yellow cattle groups from Liupan Mountain area in Ningxia.
基金Supported by National"973"Project(2011CB100802)Project of Beef Cattle Technology Innovation Team of Henan Agricultural Industry Research System(2013-14)
文摘[Objective] The paper aimed to study the improved effect of local beef cattle with Fleckvieh cattle and provide theoretical basic data for local cattle industry in central plain agricultural area in China. [Method] With 500 local beef cattle as female parents and Fleckvieh cattle as male parents,hybridization improvement was conducted via artificial insemination. The growth performance,slaughter performance,milk performance and milk components of F1 and F2 hybrids were measured. [Result] The birth body weights of F2 were significantly higher than those of local beef cow,but there was no remarkable difference between F1 and local beef cow or F1 and F2. The growth rates of F1 and F2 at different stages were higher than those of local beef cattle. The slaughter performance,such as carcass weight( P < 0. 05),dressing percentage,net meat rate( P < 0. 05),marbling score of F1 and F2 were higher than those of local beef cow. Milk production performance,such as actual milk yield,305 d corrected milk yield and 4% standard milk yield of F2 were signally higher than those of F1 and local beef cattle( P< 0. 05),and F1 was markedly higher than local beef cattle( P < 0. 05). For milk composition,although milk fat percentage,milk protein rate,lactose rate and total solids( TS) of F1 and F2 were slightly lowered compared with local beef cattle at varying degrees,they were still at high levels compared with Holstein cows.[Conclusion]Fleckvieh cattle,as a male parent,can significantly improved growth performance,slaughter performance and milk performance of offsprings. It would also increases the economic efficiency of local beef cattle by higher quality and price,as well as changing production model from beef to dual purpose of beef and milk.
文摘In the improvement process of local yellow cattle with frozen semen of beef cattle, the cows were grouped according to different cross combinations. Then, the conception rate, dystocia rate and reproductive survival rate of the cows in each group, as well as the body size and body weight of new-born calves, were ana- lyzed. The results showed that no significant differences were found in conception rate among different groups （P〉0.05）; there was no significant difference in dystocia rate between the A and C groups （P〉0.05）, but the dystocia rate in the B group was significantly different from those in the A and C groups （P〈0.05）. There were positive correlations between calf forehead width, chest circumference and the dys- tocia rate.
文摘以经产荷斯坦奶牛外周血白细胞为材料,分离Poly(A)+ RNA,反转录合成单链及双链cDNA,经酶切成平均大小为400～600 bp的片段,将患乳房炎奶牛cDNA分成2组,分别与2种不同的接头连接,再与健康奶牛cDNA进行2次消减杂交和2次抑制性PCR扩增,将第2次PCR产物与pGEM-T载体连接,转化大肠杆菌TOP10感受态细胞进行文库扩增,构建了具有高消减效率的奶牛乳房炎抗性相关cDNA文库.文库扩增后得到610个白色阳性克隆,随机挑选克隆进行PCR鉴定,插入片段主要分布在250～750 bp 之间.文库的成功构建为进一步筛选、克隆与奶牛乳房炎抗性相关的基因奠定了基础,对研究奶牛乳房炎抗性的分子机制以及乳房炎的综合防制具有重要意义.
