Walt·Whitman was one of the famous poets in American literary field. He is remembered by his masterpiece-Leaves of Grass, which composes a lot of his democratic ideas. His democratic poems and his writing styles ...Walt·Whitman was one of the famous poets in American literary field. He is remembered by his masterpiece-Leaves of Grass, which composes a lot of his democratic ideas. His democratic poems and his writing styles have their historical background which helped form his democratic ideas.展开更多
The poem O Captain! My Captain! is a good poem written by the great American poet, Walt Whitman. It is a eulogy written on the death of Abraham Lincoln. In this poem the poet expresses his sadness at the death of the ...The poem O Captain! My Captain! is a good poem written by the great American poet, Walt Whitman. It is a eulogy written on the death of Abraham Lincoln. In this poem the poet expresses his sadness at the death of the great leader and expresses his great love of his beloved leader. This paper is to have a stylistic analysis of the poem by observing its phonological, lexical, semantic and syntactic features.展开更多
Walt Whitman and Emily Dickinson are known as original geniuses in American literature. There exist both similarities and differences between Whitman and Dickinson. This paper will discuss the similarities and differe...Walt Whitman and Emily Dickinson are known as original geniuses in American literature. There exist both similarities and differences between Whitman and Dickinson. This paper will discuss the similarities and differences between these two outstanding American poets from several aspects, such as their lives, literary styles, themes and poetic techniques.展开更多
Whitman believes that people in cities should establish a harmonious relationship between humans and nature.In his urban imagination,humans are a common link in the entire ecosystem.As a part of heaven and earth,every...Whitman believes that people in cities should establish a harmonious relationship between humans and nature.In his urban imagination,humans are a common link in the entire ecosystem.As a part of heaven and earth,everything is one.Through dialogue with nature,Whitman explored that man and nature should be equal and harmonious.Whitman embodies the unique urban ecological poetics in his essay the Riverbank of the Ferry Crossing Last Winter,which contains Chinese classical thought of harmony between man and nature.展开更多
Walt Whitman has been renowned as the representative American poet of democracy. However, through an exploration of his early articles and poems, it could be found that both his political philosophy and cultural ideas...Walt Whitman has been renowned as the representative American poet of democracy. However, through an exploration of his early articles and poems, it could be found that both his political philosophy and cultural ideas were in accordance with the white identity of Americans, giving priority to the interests of white people rather than every single American resident. In this sense, it seems appropriate to consider Whitman as the spokesman for American whites.展开更多
Walt Whitman's democratic theme is rich in meaning, which finds expression in the language and form of his poetry as well as in his prose. The democratic theme establishes Whitman's reputation as a singer of d...Walt Whitman's democratic theme is rich in meaning, which finds expression in the language and form of his poetry as well as in his prose. The democratic theme establishes Whitman's reputation as a singer of democracy, who exerts powerful influence on later poets.展开更多
目的:采用分子模拟技术从宽体金线蛭来源多肽库中筛选抗凝活性多肽,结合分子对接初筛及分子动力学模拟复筛,最终通过抗凝实验验证得到宽体金线蛭抗凝活性多肽。方法:利用虚拟酶切技术对宽体金线蛭蛋白库进行酶切,得到宽体金线蛭多肽候选...目的:采用分子模拟技术从宽体金线蛭来源多肽库中筛选抗凝活性多肽,结合分子对接初筛及分子动力学模拟复筛,最终通过抗凝实验验证得到宽体金线蛭抗凝活性多肽。方法:利用虚拟酶切技术对宽体金线蛭蛋白库进行酶切,得到宽体金线蛭多肽候选库;选择凝血酶为凝血活性靶点,采用HPEPDOCK及Discovery Studio CDOCKER两种方法进行分子对接,并利用分子动力学模拟技术对分子对接初筛结果中对接结合能高的复合物进行动力学模拟复筛,通过MM-PBSA模块分析计算凝血酶-多肽复合物结合自由能;最后采用固相合成法化学合成复筛中稳定结合的宽体金线蛭多肽并进行体外抗凝活性测定。结果:经过虚拟酶切共得到3317条无毒多肽,通过分子对接进行初筛,结合分子动力学模拟复筛,得到1条结合凝血酶效果最好的目标抗凝肽,该多肽序列为QNTVGLDDFFSSYER,结合自由能为-427.506 kJ·mol^(-1)。凝血酶Arg233残基是该凝血酶-多肽复合物中介导结合相互作用的关键氨基酸。该宽体金线蛭抗凝肽在体外活性测定中确能延长凝血酶时间。结论:利用分子模拟技术有效筛选出1条宽体金线蛭抗凝活性肽,并经过抗凝实验验证,表明“模拟+实验”的活性肽筛选策略切实可行,该研究思路可为动物类中药物质基础研究提供参考。展开更多
文摘Walt·Whitman was one of the famous poets in American literary field. He is remembered by his masterpiece-Leaves of Grass, which composes a lot of his democratic ideas. His democratic poems and his writing styles have their historical background which helped form his democratic ideas.
文摘The poem O Captain! My Captain! is a good poem written by the great American poet, Walt Whitman. It is a eulogy written on the death of Abraham Lincoln. In this poem the poet expresses his sadness at the death of the great leader and expresses his great love of his beloved leader. This paper is to have a stylistic analysis of the poem by observing its phonological, lexical, semantic and syntactic features.
文摘Walt Whitman and Emily Dickinson are known as original geniuses in American literature. There exist both similarities and differences between Whitman and Dickinson. This paper will discuss the similarities and differences between these two outstanding American poets from several aspects, such as their lives, literary styles, themes and poetic techniques.
文摘Whitman believes that people in cities should establish a harmonious relationship between humans and nature.In his urban imagination,humans are a common link in the entire ecosystem.As a part of heaven and earth,everything is one.Through dialogue with nature,Whitman explored that man and nature should be equal and harmonious.Whitman embodies the unique urban ecological poetics in his essay the Riverbank of the Ferry Crossing Last Winter,which contains Chinese classical thought of harmony between man and nature.
文摘Walt Whitman has been renowned as the representative American poet of democracy. However, through an exploration of his early articles and poems, it could be found that both his political philosophy and cultural ideas were in accordance with the white identity of Americans, giving priority to the interests of white people rather than every single American resident. In this sense, it seems appropriate to consider Whitman as the spokesman for American whites.
文摘Walt Whitman's democratic theme is rich in meaning, which finds expression in the language and form of his poetry as well as in his prose. The democratic theme establishes Whitman's reputation as a singer of democracy, who exerts powerful influence on later poets.
文摘目的:采用分子模拟技术从宽体金线蛭来源多肽库中筛选抗凝活性多肽,结合分子对接初筛及分子动力学模拟复筛,最终通过抗凝实验验证得到宽体金线蛭抗凝活性多肽。方法:利用虚拟酶切技术对宽体金线蛭蛋白库进行酶切,得到宽体金线蛭多肽候选库;选择凝血酶为凝血活性靶点,采用HPEPDOCK及Discovery Studio CDOCKER两种方法进行分子对接,并利用分子动力学模拟技术对分子对接初筛结果中对接结合能高的复合物进行动力学模拟复筛,通过MM-PBSA模块分析计算凝血酶-多肽复合物结合自由能;最后采用固相合成法化学合成复筛中稳定结合的宽体金线蛭多肽并进行体外抗凝活性测定。结果:经过虚拟酶切共得到3317条无毒多肽,通过分子对接进行初筛,结合分子动力学模拟复筛,得到1条结合凝血酶效果最好的目标抗凝肽,该多肽序列为QNTVGLDDFFSSYER,结合自由能为-427.506 kJ·mol^(-1)。凝血酶Arg233残基是该凝血酶-多肽复合物中介导结合相互作用的关键氨基酸。该宽体金线蛭抗凝肽在体外活性测定中确能延长凝血酶时间。结论:利用分子模拟技术有效筛选出1条宽体金线蛭抗凝活性肽,并经过抗凝实验验证,表明“模拟+实验”的活性肽筛选策略切实可行,该研究思路可为动物类中药物质基础研究提供参考。
