This study was carried out to determine if exposure to hot environmental temperatures had a direct, detrimental effect on sperm quality. For this the effect of whole-body heat exposure on epididymal spermatozoa of lab...This study was carried out to determine if exposure to hot environmental temperatures had a direct, detrimental effect on sperm quality. For this the effect of whole-body heat exposure on epididymal spermatozoa of laboratory mice was investigated. C57BL/6 mice (n = 7) were housed in a microclimate chamber at 37℃-38℃ for 8 h per day for three consecutive days, while control mice (n = 7) were kept at 23℃-24℃. Cauda epididymal spermatozoa were obtained 16 h after the last heat treatment. The results showed that sperm numbers were similar in the two groups (P = 0.23), but after heat treatment, a significant reduction in the percentage of motile sperm was present (P 〈 0.0001). Membrane changes of the spermatozoa were investigated by staining with phycoerythrin (PE)- conjugated Annexin V, which detects exteriorization of phosphotidylserine from the inner to the outer leaflet of the sperm plasma membrane, and 7-aminoactinomycin D (7-AAD), which binds to the sperm nucleus when the plasma membrane is damaged. The percentage of spermatozoa showing positive staining with Annexin V-PE or 7-AAD or both, was significantly higher (P 〈 0.05) in heat-exposed mice compared with controls. These results show that whole-body heat exposure to 37℃-38℃ induces membrane changes in the epididymal spermatozoa of mice, which may lead to apoptosis.展开更多
Objective: We investigated the correlation between the number of circulating tumor cells(CTCs) and wholebody metabolic tumor volume(WBMTV) measured by 18 F-fluorodeoxyglucose(FDG) positron emission tomography/computed...Objective: We investigated the correlation between the number of circulating tumor cells(CTCs) and wholebody metabolic tumor volume(WBMTV) measured by 18 F-fluorodeoxyglucose(FDG) positron emission tomography/computed tomography(PET/CT).The aim was to evaluate the value of the incorporation of CTC number and WBMTV in the prognostic prediction of stage III small-cell lung cancer(SCLC).Methods: One hundred and twenty-nine patients were enrolled in this study.All patients were treated with four cycles of a platinum-based regimen and concurrent chest irradiation,followed by prophylactic cranial irradiation.Blood samples for CTC analysis were obtained from 112 patients before the initiation of chemotherapy(as a baseline),after cycle 1 and after cycle 4.CTCs were measured using the CELLSEARCH? system.The patients underwent pretreatment FDG PET/CT WBMTV,which included all malignant lesions.The Spearman rank test was used to determine the correlation among CTC counts,WBMTV and disease stage.Overall survival(OS) and progression-free survival(PFS) curves were produced using the Kaplan-Meier method,and survival differences between groups were assessed by the log-rank test.Results: The number of CTCs at baseline did not correlate with WBMTV before the initiation of therapy(P=0.241).The number of CTCs at baseline and the WBMTV before the initiation of therapy were independent relevant factors for PFS and OS.The subgroup analysis(Group A: CTC count >19.5 and a WBMTV >266.5cm~3;Group B: CTC count >19.5 and a WBMTV ≤266.5cm~3; Group C: CTC count ≤19.5 and a WBMTV >266.5cm~3;Group D: CTC count ≤19.5 and a WBMTV ≤266.5cm~3) showed that the differences were statistically significant in the median PFS(Group A vs.D,P<0.001; Group B vs.D,P=0.018; Group C vs.D,P=0.029) and in the median OS(Group A vs.D,P<0.001; Group B vs.D,P=0.012).Conclusions: CTC number and WBMTV are related to progression and death in patients with SCLC.The incorporation of CTC number and WBMTV scans can provide a detailed prognostic prediction for SCLC.展开更多
To investigate the effect of aerobic exercise combined with whole-body vibration (WBV) training on metabolic syndrome risk, and inflammatory markers, and to compare its effects on arterial stiffness and several blood ...To investigate the effect of aerobic exercise combined with whole-body vibration (WBV) training on metabolic syndrome risk, and inflammatory markers, and to compare its effects on arterial stiffness and several blood parameters related to metabolic syndrome with those of aerobic training alone. Thirty healthy participants were divided into 2 groups matched for age and body mass index (BMI). Fifteen subjects were assigned to the WBV group (WBV + aerobic training) and the other 15 to the control group (aerobic training only). All participants performed a treadmill exercise 3 days/week for 4 weeks, for 50 min after 30 min rest period. Exercise was performed at a heart rate (HR) corresponding to 60% of the HR at each individual’s maximum oxygen uptake (VO2max). Body weight and BMI after training were significantly lower than those before training in the WBV group (P < 0.01). Concerning arterial stiffness, the pulse-wave velocity after training was significantly lower than that before training in the WBV group (1048.7 ± 105.6 vs. 1008.4 ± 101.1 cm/s, P < 0.05). Meanwhile, the VO2max after training was significantly higher than that before training in the WBV group (47.7 ± 12.8 vs. 51.4 ± 14.1 mL·min-1·kg-1, P < 0.01). However, no significant differences were observed in any parameters before and after training in the control group. Our results suggest that aerobic exercise combined with WBV training may be effective in reducing arterial stiffness and improving cardiorespiratory fitness than aerobic training alone.展开更多
Objective: The research was carried out to test the influence of adaptation to passive whole body hyperthermia (PH) on physical capacity and cardio-respiratory efficiency in men-amateur athletes under the testing load...Objective: The research was carried out to test the influence of adaptation to passive whole body hyperthermia (PH) on physical capacity and cardio-respiratory efficiency in men-amateur athletes under the testing load of increasing intensity in laboratory thermal-neutral conditions. Twenty-eight amateur athletes (males, mean age 20.2 ± 2.1 y.o., game-based kinds of sports—football, handball, training experience—4 - 7 years) were randomized into two groups: experimental (14 people), who underwent 24 PH procedures, and control (14 people), who underwent 24 light training sessions in interval regime on elliptical gym apparatus. Results: Adaptation to PH was accompanied by moderate increase of aerobic efficiency and cardio-respiratory endurance in amateur athletes under thermally neutral conditions, while interval training in the control group showed no reliable changes in efficiency. Conclusion: A 10-week course of PH procedures leads to the increase of peak oxygen consumption, oxygen consumption at the level of anaerobic threshold, and heart efficiency (enhancement of oxygen pulse values), as well as efficiency of pulmonary ventilation and bronchial conductance, which we consider to be cross effects of adaptation to passive whole-body repetitive hyperthermia.展开更多
Whole-body control is beneficial for improving the disturbance adaptation of humanoid robots,since it can simultaneously optimize desired joint torque,joint acceleration,and contact force while considering whole-body ...Whole-body control is beneficial for improving the disturbance adaptation of humanoid robots,since it can simultaneously optimize desired joint torque,joint acceleration,and contact force while considering whole-body dynamics and other physical limits.However,the lack of torque feedback information prevents the position-controlled humanoids from utilizing whole-body control directly,because it enhances the difficulty of guaranteeing desired contact force which is important for maintaining stability.In this paper,a whole-body control that integrates task-space inverse dynamics and variable contact force control is proposed for position-controlled humanoids to enhance the robot’s adaptability toward the unknown disturbance.The task-space inverse dynamics generates the desired joint acceleration and contact force with the consideration of whole-body dynamics and other limits to track the references.The variable contact force control modifies references related to Center of Mass(CoM)and end effectors to ensure reasonable contact force tracking performance,thereby assuring good tracking performance of CoM and momentum to maintain robot stability.Simulations and experiments of balancing and walking under unknown disturbance have been successfully conducted on a position-controlled humanoid robot,BHR-7P3,with the proposed method.展开更多
Objective:The primary aim of this study was to assess the efficacy of whole-body diffusion-weighted imaging(WB-DWI)in detecting tumour recurrence and metastasis of gastrointestinal cancers by comparison with 18F-2-flu...Objective:The primary aim of this study was to assess the efficacy of whole-body diffusion-weighted imaging(WB-DWI)in detecting tumour recurrence and metastasis of gastrointestinal cancers by comparison with 18F-2-fluoro-2-deoxy-Dglucose positron emission tomography or computed tomography(18F-FDG-PET/CT).A secondary aim was to evaluate the change of apparent diffusion coefficient(ADC)value between metastases and normal tissues.Methods:Twenty-eight previously confirmed gastrointestinal cancer patients with suspected tumour recurrence or metastasis were recruited.WB-DWI and PET/CT images were evaluated by two radiologists and a nuclear medicine physician.Agreement between WB-DWI and PET/CT for detective efficacy was compared using kappa statistics.Additionally,diagnostic accuracy,sensitivity,specificity,negative predictive value(NPV),and positive predictive value(PPV)were also statistically analysed.ADC values between metastatic and normal tissues were compared.Results:There was no statistically significant difference(P>0.05)in the overall diagnostic performances of PET/CT(accuracy 98.9%;sensitivity 95.2%;specificity 99.8%;PPV 98.9%;NPV 98.9%)and WB-DWI(accuracy 95.9%;sensitivity 81.7%;specificity 99.1%;PPV 95.0%;NPV 96.1%).WB-DWI showed agreement with PET/CT(j=0.877)for detecting recurrence and distant metastases.A statistically significant difference in ADC value was observed between tissues of normal healthy volunteers and metastases in lymph nodes,liver and bones(P<0.05).Conclusions:WB-DWI is reliable in detecting tumour recurrence and metastasis of colorectal cancer and offers the same diagnostic performance as 18F-PET/CT without ionizing radiation.The quantitative value of ADC provides extra information to determine cancer metastasis.展开更多
Squatting is a basic movement of bipedal robots,which is essential in robotic actions like jumping or picking up objects.Due to the intrinsic complex dynamics of bipedal robots,perfect squatting motion requires high-p...Squatting is a basic movement of bipedal robots,which is essential in robotic actions like jumping or picking up objects.Due to the intrinsic complex dynamics of bipedal robots,perfect squatting motion requires high-performance motion planning and control algorithms.The standard academic solution combines model predictive control(MPC)with whole-body control(WBC),which is usually computationally expensive and difficult to implement on practical robots with limited computing resources.The real-time kinematic prediction(RKP)method is proposed,which considers upcoming reference motion trajectories and combines it with quadratic programming(QP)-based WBC.Since the WBC handles the full robot dynamics and various constraints,the RKP only needs to adopt the linear kinematics in the robot's task space and to softly constrain the desired accelerations.Then,the computational cost of derived closed-form RKP is greatly reduced.The RKP method is verified in simulation on a heavy-loaded bipedal robot.The robot makes rapid and large-amplitude squatting motions,which require close-to-limit torque outputs.Compared with the conventional QP-based WBC method,the proposed method exhibits high adaptability to rough planning,which implies much less user interference in the robot's motion planning.Furthermore,like the MPC,the proposed method can prepare for upcoming motions in advance but requires much less computation time.展开更多
Noise and whole-body vibration measurements were made in a Viking military vehicle to determine the variation that should be expected during repeat measures,the effect of speed(up to 60 km/h in 5 km/h increments),and ...Noise and whole-body vibration measurements were made in a Viking military vehicle to determine the variation that should be expected during repeat measures,the effect of speed(up to 60 km/h in 5 km/h increments),and during travel over different types of terrain(comprising concrete road,gravel track and rough cross-country).Measurements were made at various crew positions(including the driver and commander)in both the front and the rear cabs in the vehicles.Three translational axes of vibration were measured in each seat.Two speeds were investigated over road(35 km/h and 55e60 km/h)and gravel(20 km/h and 35 km/h)surfaces.The effect of varying speed of the vehicle on the measured noise and vibration magnitudes was also investigated.The highest sound pressure level(LAeq)of 104 dB(A)was measured at the commander’s standing position during travel over concrete road at 55 km/h.Higher noise levels occurred for a standing commander compared with when sitting on the seat.A maximum single axis frequency-weighted vibration magnitude of 1.0 m/s^(2) r.m.s.was measured on the driver’s seat during travel over track at 35 km/h.Higher vibration magnitudes occurred during travel over track compared with travel over road.Both noise and vibration exposure of crew within the Viking vehicle increased with increasing speed of the vehicle.展开更多
There is little information about drivers’body balance responses to combined exposure of noise and vibration.To fill the gap,this study aims to investigate the combined effects of exposure to noise and whole-body vib...There is little information about drivers’body balance responses to combined exposure of noise and vibration.To fill the gap,this study aims to investigate the combined effects of exposure to noise and whole-body vibration(WBV)on the body balance under simulated driving conditions.For this purpose,30 male participants were exposed to noise level at 85 dB(A)and two vibration levels(0.87 and 1.3 m/s^(2))in five sessions.The design of the study was repeated-measures,and it attempted to assess the effects of 40 minutes of exposure to noise and/or WBV.Moreover,the participants’fatigue was measured with the Borg scale(CR 10).The findings revealed there was a significant change in body sway after WBV and combined noise and WBV exposure(p<0.05).However,no significant difference was found in exposure to noise alone(p>0.05).The effect sizes of exposure to noise,WBV(1.3 m/s^(2)),and combined noise and WBV(1.3 m/s^(2))on body balance were 0.035,0.425,and 0.635,respectively.Also,single exposure to WBV caused more fatigue than single exposure to noise(p<0.05).Combined noise and WBV exposure descriptively caused more fatigue in comparison with the influence of WBV alone.The study concluded that the combined effects of exposure to noise and vibration are more than the sum of them.So,some synergistic effects may be observed in human body balance.It is essential to increase drivers’awareness and revise current health care interventions about new possible effects of combined exposures.展开更多
This study was to explore the optimal threshold of thyroid-stimulating hormone(TSH)-stimulated serum thyroglobulin(s-Tg) for patients who were to receive18F-fluorodeoxyglucose(18F-FDG) PET/CT scan owing to clinical su...This study was to explore the optimal threshold of thyroid-stimulating hormone(TSH)-stimulated serum thyroglobulin(s-Tg) for patients who were to receive18F-fluorodeoxyglucose(18F-FDG) PET/CT scan owing to clinical suspicion of differentiated thyroid cancer(DTC) recurrence but negative post-therapeutic 131I whole-body scan(131I-WBS). A total of 60 qualified patients underwent PET/CT scanning from October 2010 to July 2014. The receiver operating characteristic(ROC) curve analyses showed that s-Tg levels over 49 μg/L led to the highest diagnostic accuracy of PET/CT to detect recurrence, with a sensitivity of 89.5% and a specificity of 90.9%. Besides, bivariate correlation analysis showed positive correlation between s-Tg levels and the maximum standardized uptake values(SUVmax) of18F-FDG in patients with positive PET/CT scanning, suggesting a significant influence of TSH both on Tg release and uptake of18F-FDG. So, positive PET/CT imaging is expected when patients have negative 131I-WBS but s-Tg levels over 49 μg/L.展开更多
Whole Body Vibration(WBV)is a new exercise trend in fitness and performance enhancement.WBV platforms oscillate over a range of frequencies and amplitudes;however no study to date has examined the experience of percei...Whole Body Vibration(WBV)is a new exercise trend in fitness and performance enhancement.WBV platforms oscillate over a range of frequencies and amplitudes;however no study to date has examined the experience of perceived exertion with differences in vibration frequency and static or dynamic body position,nor examined the difference between sexes based on position and frequency during WBV.The purpose of this study was to assess the effects of position and frequency on perceived exertion during WBV in healthy,active adults.A convenience sample of 39 healthy young adults who participated in moderated exercise was blinded and randomly allocated to six WBV conditions composed of different frequency and position combinations.The participants received instruction,provided informed consent,participated in a warm-up session,and then reported rating of perceived exertion for each WBV condition.A 2×3 within-subjects multivariate approach ANOVA was conducted against an alpha of 0.05.A statistically significant main effect of position(p=0.004)and significant main effect of frequency(p=0.025)were found.Significant marginal means were found between the frequencies of 0 Hz and 50 Hz(p=0.007).Statistically significant differences were found between sexes for all combinations.展开更多
As the central nervous system controls whole-body motion, which involves multi-joint movement, certain problems with regard to the number of variables controlled by the central nervous system arise (i.e., the “degree...As the central nervous system controls whole-body motion, which involves multi-joint movement, certain problems with regard to the number of variables controlled by the central nervous system arise (i.e., the “degree of freedom problem”). The central nervous system solves these problems not by controlling joint movements, but rather by controlling only the task-dependent center of mass (COM) position of the whole body. Although uncontrolled joint movement should be organized in a coordinate manner to form the task-dependent COM position, it is unclear what kind of law joint coordination is organized by. Hence, in the present study, we aim to clarify the shape of joint coordination by elucidating the mutual relationship between the COM trajectory and joint movement during whole-body motion. Downward squatting motions with five trunk angles are recorded by using a 3-D motion analysis system in 8 healthy males. The COM trajectory shows a task-dependent path in all trunk conditions. The shank angle decreases with an increase in the trunk angle to produce the task-dependent COM trajectory, whereas the thigh showsd a constant angle. These findings demonstrate that the COM trajectory is constrained by biomechanical dynamics and minimum muscle torques, and that the joints are organized into a lawful coordinative structure to form the COM trajectory.展开更多
In multiple trauma, blunt carotid artery injuries (BCAIs) have occasionally been reported. However, bilateral blunt carotid artery occlusions (Grade 4 BCAIs) associated with multiple trauma are rare, and delays in dia...In multiple trauma, blunt carotid artery injuries (BCAIs) have occasionally been reported. However, bilateral blunt carotid artery occlusions (Grade 4 BCAIs) associated with multiple trauma are rare, and delays in diagnosis and treatment result in a lethal outcome. Here, we report our experience with bilateral carotid artery occlusions. A 76-year-old female suffered multiple traumas in a motor vehicle accident. On arrival at our hospital, she presented in a coma, with left mydriasis and unreactive pupils. Computed tomography (CT) showed bifrontal intracranial epidural hematoma and fractures of the facial bone and anterior skull base, and osteoplastic craniotomy was urgently undertaken for the epidural hematoma. However, the comatose state and unreactive pupils persisted during the post-operative course. Serial head CT findings showed progressive bilateral ischemic changes, and radiological examinations revealed bilateral internal carotid artery occlusions. We speculated that bilateral Grade 4 BCAIs had induced progressive cerebral infarctions. The patient partially responded to anticoagulation therapy with heparin infusion, but died of multiple organ failure on day 15. When bilateral progressive ischemic changes are observed in a patient with severe traumatic brain injury, bilateral Grade 4 BCAIs should be considered in the differential diagnosis. CT angiography as part of whole-body CT at admission may be effective for preventing delays in diagnosis and treatment of bilateral Grade 4 BCAIs.展开更多
Legged locomotion poses significant challenges due to its nonlinear,underactuated and hybrid dynamic properties.These challenges are exacerbated by the high-speed motion and presence of aerial phases in dynamic legged...Legged locomotion poses significant challenges due to its nonlinear,underactuated and hybrid dynamic properties.These challenges are exacerbated by the high-speed motion and presence of aerial phases in dynamic legged locomotion,which highlights the requirement for online planning based on current states to cope with uncertainty and disturbances.This article proposes a real-time planning and control framework integrating motion planning and whole-body control.In the framework,the designed motion planner allows a wider body rotation range and fast reactive behaviors based on the 3-D single rigid body model.In addition,the combination of a Bézier curve based trajectory interpolator and a heuristic-based foothold planner helps generate continuous and smooth foot trajectories.The developed whole-body controller uses hierarchical quadratic optimization coupled with the full system dynamics,which ensures tasks are prioritized based on importance and joint commands are physically feasible.The performance of the framework is successfully validated in experiments with a torque-controlled quadrupedal robot for generating dynamic motions.展开更多
ObjectiveTo evaluate whole-body,extremity and eye-lens occupational dose equivalents of ionizing radiation to Occupationally Exposed Workers(OEWs)in Gabon,monitored by the national Individual Monitoring Service(IMS),i...ObjectiveTo evaluate whole-body,extremity and eye-lens occupational dose equivalents of ionizing radiation to Occupationally Exposed Workers(OEWs)in Gabon,monitored by the national Individual Monitoring Service(IMS),in the medical practice.MethodsA total of 816 dose records for 205 OEWs were evaluated.Doses were collected for an eight-year period,from 2013 to 2020.Twelve public and private hospitals were involved,including a cancer centre where radiotherapy(RT)and nuclear medicine(NM)procedures are regularly performed.OEWs were monitored,on a monthly or quarterly basis,for whole-body dose and extremity dose using an optically stimulated luminescence(OSL)dosimeter worn on the torso or the wrist,as appropriate.Eye-lens dose was estimated from whole-body dose results.ResultsThe whole-body dose distribution was skewed and the zero dose was associated with a probability of 47%.Some recorded doses were associated with estimated X-ray energies above those typically encountered in diagnostic radiology,thus suggesting that some doses attributed to workers and believed to be due to X-rays may in fact be due to background radiation(false positive dose records).For the period 2013–2020,annual doses to OEWs in diagnostic radiology(DR),NM and RT departments were 0.40 mSv,0.38 mSv and 0.09 mSv,respectively.For the same period,the mean annual extremity and eye-lens doses were 0.78 mSv(range:0–5.24mSv)and 0.35 mSv(range:0–3.74mSv),respectively.ConclusionWith little effort by the national IMS and the regulatory body,annual whole-body doses to workers can be kept well below 6mSv,thus efficiently contributing to the optimization of worker's protection.However,extremity doses may in fact be greater than those reported for OEWs in the NM department.Finally,eye-lens doses were well below the new annual dose limit of 20 mSv,thus demonstrating that,in the current situation,OEWs in the NM department do not require routine eye lens dose monitoring.Rather,whole-body dose may be used as an indicator of eye-lens dose.展开更多
The imaging mass spectrometry(IMS) technology has experienced a rapid development in recent years.A new IMS technology which is based on air flow assisted ionization(AFAI) was reported.It allows for the convenient...The imaging mass spectrometry(IMS) technology has experienced a rapid development in recent years.A new IMS technology which is based on air flow assisted ionization(AFAI) was reported.It allows for the convenient pretreatment of the samples and can image a large area of sample in a single measurement with high sensitivity.The AFAI in DESI mode was used as the ion source in this paper.The new IMS method is named AFADESI-IMS.The adoption of assisted air flow makes the sample pretreatment easy and convenient.An optimization of the distance between the ion transport tube and MS orifice increases the sensitivity of the system.For data processing,a program based on MATLAB with the function of numerical analysis was developed.A theoretical imaging resolution of a few hundred microns can be achieved.The composite AFAI-IMS images of different target analytes were imaged with high sensitivity.A typical AFAI-IMS image of the whole-body section of a rat was obtained in a single analytical measurement.The ability to image a large area for relevant samples in a single measurement with high sensitivity and repeatability is a significant advantage.The method has enormous potentials in the MS imaging of large and complicated samples.展开更多
Imaging dynamics at different temporal and spatial scales is essential for understanding the biological complexity of living organisms,disease state and progression.Optoacoustic imaging has been shown to offer exclusi...Imaging dynamics at different temporal and spatial scales is essential for understanding the biological complexity of living organisms,disease state and progression.Optoacoustic imaging has been shown to offer exclusive applicability across multiple scales with excellent optical contrast and high resolution in deep-tissue observations.Yet,efficient visualization of multi-scale dynamics remained difficult with state-of-the-art systems due to inefficient trade-offs between image acquisition time and effective field of view.Herein,we introduce the spiral volumetric optoacoustic tomography technique that provides spectrally enriched highresolution contrast across multiple spatiotemporal scales.In vivo experiments in mice demonstrate a wide range of dynamic imaging capabilities,from three-dimensional high-frame-rate visualization of moving organs and contrast agent kinetics in selected areas to whole-body longitudinal studies with unprecedented image quality.The newly introduced paradigm shift in imaging of multi-scale dynamics adds to the multifarious advantages provided by the optoacoustic technology for structural,functional and molecular imaging.展开更多
Objective:To determine the effects of Hydrangeae Dulcis Folium(EHDF)on physical stress,changes in the whole-body cortisol level and behaviour in zebrafish(Danio rerio).Methods:One hundred and seventy-four fish were ra...Objective:To determine the effects of Hydrangeae Dulcis Folium(EHDF)on physical stress,changes in the whole-body cortisol level and behaviour in zebrafish(Danio rerio).Methods:One hundred and seventy-four fish were randomly divided into 4[adrenocorticotropin hormone(ACTH)challenge test:4 fish per group]or 6 groups(behavioural test:10–12 fish per group,whole-body cortisol:4 fish per group).Net handling stress(NHS)was used to induce physical stress.Fish were treated with vehicle or EHDF(5–20 mg/L)for 6 min before they were exposed to stress.And then,fish were sacrificed for collecting body fluid from whole-body or conducted behavioural tests,including novel tank test and open field test,and were evaluated to observe anxiety-like behaviours and locomotion.In addition,to elucidate the mode of action of the anti-stress effects of EHDF,ACTH(0.2 IU/g,i.p.)challenge test was performed.Results:The increased anxiety-like behaviours in novel tank test and open field test under stress were prevented by treatment with EHDF at 5–20 mg/L(P<0.05).Moreover,compared with the unstressed group,which was not treated with NHS,the whole-body cortisol level was significantly increased by treatment with NHS(P<0.05).Compared with the NHS-treated stressed control group,pre-treatment with EHDF at concentrations of 5–20 mg/L for 6 min significantly prevented the NHS-increased whole-body cortisol level(P<0.05).In addition,ACTH challenge test showed that EHDF completely blocked the effects of ACTH on cortisol secretion(P<0.05).Conclusion:EHDF may be a good antistress candidate and its mechanism of action may be related to its positive effects on cortisol release.展开更多
Alzheimer’s disease(AD)is the most common cause of dementia,afecting approximately 40 million people worldwide.The inefectiveness of the available pharmacological treatments against AD has fostered researchers to foc...Alzheimer’s disease(AD)is the most common cause of dementia,afecting approximately 40 million people worldwide.The inefectiveness of the available pharmacological treatments against AD has fostered researchers to focus on alternative strategies to overcome this challenge.Mechanical vibrations delivered in diferent stimulation modes have been associated with marked improvements in cognitive and physical performance in both demented and nondemented elderly.Some of the mechanical-based stimulation modalities in eforts are earlier whole-body vibration,transcranial ultrasound stimulation with microbubble injection,and more recently,auditory stimulation.However,there is a huge variety of treatment specifcations,and in many cases,conficting results are reported.In this review,a search on Scopus,PubMed,and Web of Science databases was performed,resulting in 37 papers.These studies suggest that mechanical vibrations delivered through diferent stimulation modes are efective in attenuating many parameters of AD pathology including functional connectivity and neuronal circuit integrity defcits in the brains of AD patients,as well as in subjects with cognitive decline and non-demented older adults.Despite the evolving preclinical and clinical evidence on these therapeutic modalities,their translation into clinical practice is not consolidated yet.Thus,this comprehensive and critical systematic review aims to address the most important gaps in the reviewed protocols and propose optimal regimens for future clinical application.展开更多
文摘This study was carried out to determine if exposure to hot environmental temperatures had a direct, detrimental effect on sperm quality. For this the effect of whole-body heat exposure on epididymal spermatozoa of laboratory mice was investigated. C57BL/6 mice (n = 7) were housed in a microclimate chamber at 37℃-38℃ for 8 h per day for three consecutive days, while control mice (n = 7) were kept at 23℃-24℃. Cauda epididymal spermatozoa were obtained 16 h after the last heat treatment. The results showed that sperm numbers were similar in the two groups (P = 0.23), but after heat treatment, a significant reduction in the percentage of motile sperm was present (P 〈 0.0001). Membrane changes of the spermatozoa were investigated by staining with phycoerythrin (PE)- conjugated Annexin V, which detects exteriorization of phosphotidylserine from the inner to the outer leaflet of the sperm plasma membrane, and 7-aminoactinomycin D (7-AAD), which binds to the sperm nucleus when the plasma membrane is damaged. The percentage of spermatozoa showing positive staining with Annexin V-PE or 7-AAD or both, was significantly higher (P 〈 0.05) in heat-exposed mice compared with controls. These results show that whole-body heat exposure to 37℃-38℃ induces membrane changes in the epididymal spermatozoa of mice, which may lead to apoptosis.
基金supported by a grant from the National Health and Family Planning Commission of China(No.201402011)
文摘Objective: We investigated the correlation between the number of circulating tumor cells(CTCs) and wholebody metabolic tumor volume(WBMTV) measured by 18 F-fluorodeoxyglucose(FDG) positron emission tomography/computed tomography(PET/CT).The aim was to evaluate the value of the incorporation of CTC number and WBMTV in the prognostic prediction of stage III small-cell lung cancer(SCLC).Methods: One hundred and twenty-nine patients were enrolled in this study.All patients were treated with four cycles of a platinum-based regimen and concurrent chest irradiation,followed by prophylactic cranial irradiation.Blood samples for CTC analysis were obtained from 112 patients before the initiation of chemotherapy(as a baseline),after cycle 1 and after cycle 4.CTCs were measured using the CELLSEARCH? system.The patients underwent pretreatment FDG PET/CT WBMTV,which included all malignant lesions.The Spearman rank test was used to determine the correlation among CTC counts,WBMTV and disease stage.Overall survival(OS) and progression-free survival(PFS) curves were produced using the Kaplan-Meier method,and survival differences between groups were assessed by the log-rank test.Results: The number of CTCs at baseline did not correlate with WBMTV before the initiation of therapy(P=0.241).The number of CTCs at baseline and the WBMTV before the initiation of therapy were independent relevant factors for PFS and OS.The subgroup analysis(Group A: CTC count >19.5 and a WBMTV >266.5cm~3;Group B: CTC count >19.5 and a WBMTV ≤266.5cm~3; Group C: CTC count ≤19.5 and a WBMTV >266.5cm~3;Group D: CTC count ≤19.5 and a WBMTV ≤266.5cm~3) showed that the differences were statistically significant in the median PFS(Group A vs.D,P<0.001; Group B vs.D,P=0.018; Group C vs.D,P=0.029) and in the median OS(Group A vs.D,P<0.001; Group B vs.D,P=0.012).Conclusions: CTC number and WBMTV are related to progression and death in patients with SCLC.The incorporation of CTC number and WBMTV scans can provide a detailed prognostic prediction for SCLC.
文摘To investigate the effect of aerobic exercise combined with whole-body vibration (WBV) training on metabolic syndrome risk, and inflammatory markers, and to compare its effects on arterial stiffness and several blood parameters related to metabolic syndrome with those of aerobic training alone. Thirty healthy participants were divided into 2 groups matched for age and body mass index (BMI). Fifteen subjects were assigned to the WBV group (WBV + aerobic training) and the other 15 to the control group (aerobic training only). All participants performed a treadmill exercise 3 days/week for 4 weeks, for 50 min after 30 min rest period. Exercise was performed at a heart rate (HR) corresponding to 60% of the HR at each individual’s maximum oxygen uptake (VO2max). Body weight and BMI after training were significantly lower than those before training in the WBV group (P < 0.01). Concerning arterial stiffness, the pulse-wave velocity after training was significantly lower than that before training in the WBV group (1048.7 ± 105.6 vs. 1008.4 ± 101.1 cm/s, P < 0.05). Meanwhile, the VO2max after training was significantly higher than that before training in the WBV group (47.7 ± 12.8 vs. 51.4 ± 14.1 mL·min-1·kg-1, P < 0.01). However, no significant differences were observed in any parameters before and after training in the control group. Our results suggest that aerobic exercise combined with WBV training may be effective in reducing arterial stiffness and improving cardiorespiratory fitness than aerobic training alone.
文摘Objective: The research was carried out to test the influence of adaptation to passive whole body hyperthermia (PH) on physical capacity and cardio-respiratory efficiency in men-amateur athletes under the testing load of increasing intensity in laboratory thermal-neutral conditions. Twenty-eight amateur athletes (males, mean age 20.2 ± 2.1 y.o., game-based kinds of sports—football, handball, training experience—4 - 7 years) were randomized into two groups: experimental (14 people), who underwent 24 PH procedures, and control (14 people), who underwent 24 light training sessions in interval regime on elliptical gym apparatus. Results: Adaptation to PH was accompanied by moderate increase of aerobic efficiency and cardio-respiratory endurance in amateur athletes under thermally neutral conditions, while interval training in the control group showed no reliable changes in efficiency. Conclusion: A 10-week course of PH procedures leads to the increase of peak oxygen consumption, oxygen consumption at the level of anaerobic threshold, and heart efficiency (enhancement of oxygen pulse values), as well as efficiency of pulmonary ventilation and bronchial conductance, which we consider to be cross effects of adaptation to passive whole-body repetitive hyperthermia.
基金This work was supported in part by the National Natural Science Foundation of China under Grant 62073041 and 61973039in part by the Beijing Municipal Science and Technology Project under Grant Z221100000222013in part by the“111”Project under Grant B08043.
文摘Whole-body control is beneficial for improving the disturbance adaptation of humanoid robots,since it can simultaneously optimize desired joint torque,joint acceleration,and contact force while considering whole-body dynamics and other physical limits.However,the lack of torque feedback information prevents the position-controlled humanoids from utilizing whole-body control directly,because it enhances the difficulty of guaranteeing desired contact force which is important for maintaining stability.In this paper,a whole-body control that integrates task-space inverse dynamics and variable contact force control is proposed for position-controlled humanoids to enhance the robot’s adaptability toward the unknown disturbance.The task-space inverse dynamics generates the desired joint acceleration and contact force with the consideration of whole-body dynamics and other limits to track the references.The variable contact force control modifies references related to Center of Mass(CoM)and end effectors to ensure reasonable contact force tracking performance,thereby assuring good tracking performance of CoM and momentum to maintain robot stability.Simulations and experiments of balancing and walking under unknown disturbance have been successfully conducted on a position-controlled humanoid robot,BHR-7P3,with the proposed method.
文摘Objective:The primary aim of this study was to assess the efficacy of whole-body diffusion-weighted imaging(WB-DWI)in detecting tumour recurrence and metastasis of gastrointestinal cancers by comparison with 18F-2-fluoro-2-deoxy-Dglucose positron emission tomography or computed tomography(18F-FDG-PET/CT).A secondary aim was to evaluate the change of apparent diffusion coefficient(ADC)value between metastases and normal tissues.Methods:Twenty-eight previously confirmed gastrointestinal cancer patients with suspected tumour recurrence or metastasis were recruited.WB-DWI and PET/CT images were evaluated by two radiologists and a nuclear medicine physician.Agreement between WB-DWI and PET/CT for detective efficacy was compared using kappa statistics.Additionally,diagnostic accuracy,sensitivity,specificity,negative predictive value(NPV),and positive predictive value(PPV)were also statistically analysed.ADC values between metastatic and normal tissues were compared.Results:There was no statistically significant difference(P>0.05)in the overall diagnostic performances of PET/CT(accuracy 98.9%;sensitivity 95.2%;specificity 99.8%;PPV 98.9%;NPV 98.9%)and WB-DWI(accuracy 95.9%;sensitivity 81.7%;specificity 99.1%;PPV 95.0%;NPV 96.1%).WB-DWI showed agreement with PET/CT(j=0.877)for detecting recurrence and distant metastases.A statistically significant difference in ADC value was observed between tissues of normal healthy volunteers and metastases in lymph nodes,liver and bones(P<0.05).Conclusions:WB-DWI is reliable in detecting tumour recurrence and metastasis of colorectal cancer and offers the same diagnostic performance as 18F-PET/CT without ionizing radiation.The quantitative value of ADC provides extra information to determine cancer metastasis.
基金Science and Technology Innovation 2030-Key Project,Grant/Award Number:2021ZD0201402。
文摘Squatting is a basic movement of bipedal robots,which is essential in robotic actions like jumping or picking up objects.Due to the intrinsic complex dynamics of bipedal robots,perfect squatting motion requires high-performance motion planning and control algorithms.The standard academic solution combines model predictive control(MPC)with whole-body control(WBC),which is usually computationally expensive and difficult to implement on practical robots with limited computing resources.The real-time kinematic prediction(RKP)method is proposed,which considers upcoming reference motion trajectories and combines it with quadratic programming(QP)-based WBC.Since the WBC handles the full robot dynamics and various constraints,the RKP only needs to adopt the linear kinematics in the robot's task space and to softly constrain the desired accelerations.Then,the computational cost of derived closed-form RKP is greatly reduced.The RKP method is verified in simulation on a heavy-loaded bipedal robot.The robot makes rapid and large-amplitude squatting motions,which require close-to-limit torque outputs.Compared with the conventional QP-based WBC method,the proposed method exhibits high adaptability to rough planning,which implies much less user interference in the robot's motion planning.Furthermore,like the MPC,the proposed method can prepare for upcoming motions in advance but requires much less computation time.
文摘Noise and whole-body vibration measurements were made in a Viking military vehicle to determine the variation that should be expected during repeat measures,the effect of speed(up to 60 km/h in 5 km/h increments),and during travel over different types of terrain(comprising concrete road,gravel track and rough cross-country).Measurements were made at various crew positions(including the driver and commander)in both the front and the rear cabs in the vehicles.Three translational axes of vibration were measured in each seat.Two speeds were investigated over road(35 km/h and 55e60 km/h)and gravel(20 km/h and 35 km/h)surfaces.The effect of varying speed of the vehicle on the measured noise and vibration magnitudes was also investigated.The highest sound pressure level(LAeq)of 104 dB(A)was measured at the commander’s standing position during travel over concrete road at 55 km/h.Higher noise levels occurred for a standing commander compared with when sitting on the seat.A maximum single axis frequency-weighted vibration magnitude of 1.0 m/s^(2) r.m.s.was measured on the driver’s seat during travel over track at 35 km/h.Higher vibration magnitudes occurred during travel over track compared with travel over road.Both noise and vibration exposure of crew within the Viking vehicle increased with increasing speed of the vehicle.
基金This study was financially supported by Research Deputy of Hamadan University of Medical Sciences(Grant No.9802241621).We would like to appreciate the students of Hamadan University of Medical Sciences for their participation in this project。
文摘There is little information about drivers’body balance responses to combined exposure of noise and vibration.To fill the gap,this study aims to investigate the combined effects of exposure to noise and whole-body vibration(WBV)on the body balance under simulated driving conditions.For this purpose,30 male participants were exposed to noise level at 85 dB(A)and two vibration levels(0.87 and 1.3 m/s^(2))in five sessions.The design of the study was repeated-measures,and it attempted to assess the effects of 40 minutes of exposure to noise and/or WBV.Moreover,the participants’fatigue was measured with the Borg scale(CR 10).The findings revealed there was a significant change in body sway after WBV and combined noise and WBV exposure(p<0.05).However,no significant difference was found in exposure to noise alone(p>0.05).The effect sizes of exposure to noise,WBV(1.3 m/s^(2)),and combined noise and WBV(1.3 m/s^(2))on body balance were 0.035,0.425,and 0.635,respectively.Also,single exposure to WBV caused more fatigue than single exposure to noise(p<0.05).Combined noise and WBV exposure descriptively caused more fatigue in comparison with the influence of WBV alone.The study concluded that the combined effects of exposure to noise and vibration are more than the sum of them.So,some synergistic effects may be observed in human body balance.It is essential to increase drivers’awareness and revise current health care interventions about new possible effects of combined exposures.
基金supported by Shanghai Municipal Commission of Health and Family Planning of China(No.20134339)
文摘This study was to explore the optimal threshold of thyroid-stimulating hormone(TSH)-stimulated serum thyroglobulin(s-Tg) for patients who were to receive18F-fluorodeoxyglucose(18F-FDG) PET/CT scan owing to clinical suspicion of differentiated thyroid cancer(DTC) recurrence but negative post-therapeutic 131I whole-body scan(131I-WBS). A total of 60 qualified patients underwent PET/CT scanning from October 2010 to July 2014. The receiver operating characteristic(ROC) curve analyses showed that s-Tg levels over 49 μg/L led to the highest diagnostic accuracy of PET/CT to detect recurrence, with a sensitivity of 89.5% and a specificity of 90.9%. Besides, bivariate correlation analysis showed positive correlation between s-Tg levels and the maximum standardized uptake values(SUVmax) of18F-FDG in patients with positive PET/CT scanning, suggesting a significant influence of TSH both on Tg release and uptake of18F-FDG. So, positive PET/CT imaging is expected when patients have negative 131I-WBS but s-Tg levels over 49 μg/L.
文摘Whole Body Vibration(WBV)is a new exercise trend in fitness and performance enhancement.WBV platforms oscillate over a range of frequencies and amplitudes;however no study to date has examined the experience of perceived exertion with differences in vibration frequency and static or dynamic body position,nor examined the difference between sexes based on position and frequency during WBV.The purpose of this study was to assess the effects of position and frequency on perceived exertion during WBV in healthy,active adults.A convenience sample of 39 healthy young adults who participated in moderated exercise was blinded and randomly allocated to six WBV conditions composed of different frequency and position combinations.The participants received instruction,provided informed consent,participated in a warm-up session,and then reported rating of perceived exertion for each WBV condition.A 2×3 within-subjects multivariate approach ANOVA was conducted against an alpha of 0.05.A statistically significant main effect of position(p=0.004)and significant main effect of frequency(p=0.025)were found.Significant marginal means were found between the frequencies of 0 Hz and 50 Hz(p=0.007).Statistically significant differences were found between sexes for all combinations.
文摘As the central nervous system controls whole-body motion, which involves multi-joint movement, certain problems with regard to the number of variables controlled by the central nervous system arise (i.e., the “degree of freedom problem”). The central nervous system solves these problems not by controlling joint movements, but rather by controlling only the task-dependent center of mass (COM) position of the whole body. Although uncontrolled joint movement should be organized in a coordinate manner to form the task-dependent COM position, it is unclear what kind of law joint coordination is organized by. Hence, in the present study, we aim to clarify the shape of joint coordination by elucidating the mutual relationship between the COM trajectory and joint movement during whole-body motion. Downward squatting motions with five trunk angles are recorded by using a 3-D motion analysis system in 8 healthy males. The COM trajectory shows a task-dependent path in all trunk conditions. The shank angle decreases with an increase in the trunk angle to produce the task-dependent COM trajectory, whereas the thigh showsd a constant angle. These findings demonstrate that the COM trajectory is constrained by biomechanical dynamics and minimum muscle torques, and that the joints are organized into a lawful coordinative structure to form the COM trajectory.
文摘In multiple trauma, blunt carotid artery injuries (BCAIs) have occasionally been reported. However, bilateral blunt carotid artery occlusions (Grade 4 BCAIs) associated with multiple trauma are rare, and delays in diagnosis and treatment result in a lethal outcome. Here, we report our experience with bilateral carotid artery occlusions. A 76-year-old female suffered multiple traumas in a motor vehicle accident. On arrival at our hospital, she presented in a coma, with left mydriasis and unreactive pupils. Computed tomography (CT) showed bifrontal intracranial epidural hematoma and fractures of the facial bone and anterior skull base, and osteoplastic craniotomy was urgently undertaken for the epidural hematoma. However, the comatose state and unreactive pupils persisted during the post-operative course. Serial head CT findings showed progressive bilateral ischemic changes, and radiological examinations revealed bilateral internal carotid artery occlusions. We speculated that bilateral Grade 4 BCAIs had induced progressive cerebral infarctions. The patient partially responded to anticoagulation therapy with heparin infusion, but died of multiple organ failure on day 15. When bilateral progressive ischemic changes are observed in a patient with severe traumatic brain injury, bilateral Grade 4 BCAIs should be considered in the differential diagnosis. CT angiography as part of whole-body CT at admission may be effective for preventing delays in diagnosis and treatment of bilateral Grade 4 BCAIs.
基金supported by the China Scholarship Council under Grant[2020]06120186the Engineering and Physical Sciences Research Council[Grant number EP/V026801/2]+1 种基金the Advanced Machinery and Productivity Institute[Innovate UK project number 84646]the National Natural Science Foundation of China under Grant 52175011.
文摘Legged locomotion poses significant challenges due to its nonlinear,underactuated and hybrid dynamic properties.These challenges are exacerbated by the high-speed motion and presence of aerial phases in dynamic legged locomotion,which highlights the requirement for online planning based on current states to cope with uncertainty and disturbances.This article proposes a real-time planning and control framework integrating motion planning and whole-body control.In the framework,the designed motion planner allows a wider body rotation range and fast reactive behaviors based on the 3-D single rigid body model.In addition,the combination of a Bézier curve based trajectory interpolator and a heuristic-based foothold planner helps generate continuous and smooth foot trajectories.The developed whole-body controller uses hierarchical quadratic optimization coupled with the full system dynamics,which ensures tasks are prioritized based on importance and joint commands are physically feasible.The performance of the framework is successfully validated in experiments with a torque-controlled quadrupedal robot for generating dynamic motions.
基金This work has received operational support from the National Regulatory Authority of Gabon.
文摘ObjectiveTo evaluate whole-body,extremity and eye-lens occupational dose equivalents of ionizing radiation to Occupationally Exposed Workers(OEWs)in Gabon,monitored by the national Individual Monitoring Service(IMS),in the medical practice.MethodsA total of 816 dose records for 205 OEWs were evaluated.Doses were collected for an eight-year period,from 2013 to 2020.Twelve public and private hospitals were involved,including a cancer centre where radiotherapy(RT)and nuclear medicine(NM)procedures are regularly performed.OEWs were monitored,on a monthly or quarterly basis,for whole-body dose and extremity dose using an optically stimulated luminescence(OSL)dosimeter worn on the torso or the wrist,as appropriate.Eye-lens dose was estimated from whole-body dose results.ResultsThe whole-body dose distribution was skewed and the zero dose was associated with a probability of 47%.Some recorded doses were associated with estimated X-ray energies above those typically encountered in diagnostic radiology,thus suggesting that some doses attributed to workers and believed to be due to X-rays may in fact be due to background radiation(false positive dose records).For the period 2013–2020,annual doses to OEWs in diagnostic radiology(DR),NM and RT departments were 0.40 mSv,0.38 mSv and 0.09 mSv,respectively.For the same period,the mean annual extremity and eye-lens doses were 0.78 mSv(range:0–5.24mSv)and 0.35 mSv(range:0–3.74mSv),respectively.ConclusionWith little effort by the national IMS and the regulatory body,annual whole-body doses to workers can be kept well below 6mSv,thus efficiently contributing to the optimization of worker's protection.However,extremity doses may in fact be greater than those reported for OEWs in the NM department.Finally,eye-lens doses were well below the new annual dose limit of 20 mSv,thus demonstrating that,in the current situation,OEWs in the NM department do not require routine eye lens dose monitoring.Rather,whole-body dose may be used as an indicator of eye-lens dose.
基金financially supported by the National Instrumentation Program (No. 2011YQ17006702)the National Natural Science Foundation of China (No. 21027013 and No. 81102413)Fundamental Research Program of Shenzhen (No. JC201005280634A)
文摘The imaging mass spectrometry(IMS) technology has experienced a rapid development in recent years.A new IMS technology which is based on air flow assisted ionization(AFAI) was reported.It allows for the convenient pretreatment of the samples and can image a large area of sample in a single measurement with high sensitivity.The AFAI in DESI mode was used as the ion source in this paper.The new IMS method is named AFADESI-IMS.The adoption of assisted air flow makes the sample pretreatment easy and convenient.An optimization of the distance between the ion transport tube and MS orifice increases the sensitivity of the system.For data processing,a program based on MATLAB with the function of numerical analysis was developed.A theoretical imaging resolution of a few hundred microns can be achieved.The composite AFAI-IMS images of different target analytes were imaged with high sensitivity.A typical AFAI-IMS image of the whole-body section of a rat was obtained in a single analytical measurement.The ability to image a large area for relevant samples in a single measurement with high sensitivity and repeatability is a significant advantage.The method has enormous potentials in the MS imaging of large and complicated samples.
基金funding from the European Research Council under grant agreements ERC-2010-StG-260991 and ERC-2015-CoG-682379the Human Frontier Science Program(HFSP)Grant RGY0070/2016.
文摘Imaging dynamics at different temporal and spatial scales is essential for understanding the biological complexity of living organisms,disease state and progression.Optoacoustic imaging has been shown to offer exclusive applicability across multiple scales with excellent optical contrast and high resolution in deep-tissue observations.Yet,efficient visualization of multi-scale dynamics remained difficult with state-of-the-art systems due to inefficient trade-offs between image acquisition time and effective field of view.Herein,we introduce the spiral volumetric optoacoustic tomography technique that provides spectrally enriched highresolution contrast across multiple spatiotemporal scales.In vivo experiments in mice demonstrate a wide range of dynamic imaging capabilities,from three-dimensional high-frame-rate visualization of moving organs and contrast agent kinetics in selected areas to whole-body longitudinal studies with unprecedented image quality.The newly introduced paradigm shift in imaging of multi-scale dynamics adds to the multifarious advantages provided by the optoacoustic technology for structural,functional and molecular imaging.
基金Supported by the Ministry of Trade,Industry&Energy(MOTIE)Korea Institute for Advancement of Technology(KIAT)through the Encouragement Program for the Industries of Economic Cooperation Region
文摘Objective:To determine the effects of Hydrangeae Dulcis Folium(EHDF)on physical stress,changes in the whole-body cortisol level and behaviour in zebrafish(Danio rerio).Methods:One hundred and seventy-four fish were randomly divided into 4[adrenocorticotropin hormone(ACTH)challenge test:4 fish per group]or 6 groups(behavioural test:10–12 fish per group,whole-body cortisol:4 fish per group).Net handling stress(NHS)was used to induce physical stress.Fish were treated with vehicle or EHDF(5–20 mg/L)for 6 min before they were exposed to stress.And then,fish were sacrificed for collecting body fluid from whole-body or conducted behavioural tests,including novel tank test and open field test,and were evaluated to observe anxiety-like behaviours and locomotion.In addition,to elucidate the mode of action of the anti-stress effects of EHDF,ACTH(0.2 IU/g,i.p.)challenge test was performed.Results:The increased anxiety-like behaviours in novel tank test and open field test under stress were prevented by treatment with EHDF at 5–20 mg/L(P<0.05).Moreover,compared with the unstressed group,which was not treated with NHS,the whole-body cortisol level was significantly increased by treatment with NHS(P<0.05).Compared with the NHS-treated stressed control group,pre-treatment with EHDF at concentrations of 5–20 mg/L for 6 min significantly prevented the NHS-increased whole-body cortisol level(P<0.05).In addition,ACTH challenge test showed that EHDF completely blocked the effects of ACTH on cortisol secretion(P<0.05).Conclusion:EHDF may be a good antistress candidate and its mechanism of action may be related to its positive effects on cortisol release.
基金This systematic review was supported by FCT(Fundação para a Ciência e Tecnologia)through the grant with reference SFRH/BD/09375/2020,and in the scope of the projects UIDB/04436/2020,UIDP/04436/2020,and NORTE-01-0145-FEDER-000023funded by the European Fund for Regional Development(FEDER)of the Operational Programme for Competitiveness and Internationalization(POCI),by Portugal 2020。
文摘Alzheimer’s disease(AD)is the most common cause of dementia,afecting approximately 40 million people worldwide.The inefectiveness of the available pharmacological treatments against AD has fostered researchers to focus on alternative strategies to overcome this challenge.Mechanical vibrations delivered in diferent stimulation modes have been associated with marked improvements in cognitive and physical performance in both demented and nondemented elderly.Some of the mechanical-based stimulation modalities in eforts are earlier whole-body vibration,transcranial ultrasound stimulation with microbubble injection,and more recently,auditory stimulation.However,there is a huge variety of treatment specifcations,and in many cases,conficting results are reported.In this review,a search on Scopus,PubMed,and Web of Science databases was performed,resulting in 37 papers.These studies suggest that mechanical vibrations delivered through diferent stimulation modes are efective in attenuating many parameters of AD pathology including functional connectivity and neuronal circuit integrity defcits in the brains of AD patients,as well as in subjects with cognitive decline and non-demented older adults.Despite the evolving preclinical and clinical evidence on these therapeutic modalities,their translation into clinical practice is not consolidated yet.Thus,this comprehensive and critical systematic review aims to address the most important gaps in the reviewed protocols and propose optimal regimens for future clinical application.