适于GlobalAllomeTree国际数据平台的标准化中国主要树种树高-胸径方程研建

GlobalAllomeTree作为共享异速方程的国际网络平台,逐渐受到全球高度关注。当前,为促进该项国际合作,针对当前该平台缺乏中国主要树种生长异速方程的现状,系统性更新标准化中国主要树种树高-胸径方程。由于树冠和下部灌木及草丛遮挡,树高相对于胸径测量具有一定的难度,因此需要使用数学工具进行计算。选取了36个树种为材料构建树高-胸径关系方程,以全国主要树种的二元材积模型、各地区一元材积表为基础材料,以取样径阶为1 cm间隔所生成1692组树高-胸径数据作为建立方程样本,1238组外业调查数据为验证样本。建模结果表明:36个主要树种的1692组树高-胸径数据建立的全国通用性树高-胸径方程拟合相关系数(R2)为0.801,方程拟合结果较好,说明可以通过测定胸径,带入树高(H,m)-胸径(D,cm)方程(H=aDb)预估树高;对36个主要树种的树高-胸径方程进行拟合,决定系数R2值均大于0.916,平均误差(ME)、平均绝对误差(MAE)和均方根误差(RMSE)相对较小,方程整体精度较高,可广泛推广;将外业采集的1238组树高-胸径数据,根据36个主要树种树高-胸径方程拟合公式及参数估计值a、b进行方程精度验证,方程预测的平均相对误差为16.86%,在误差允许范围内,并且模型形式规范,可为GlobalAllomeTree平台用户提供科学参考。

基于FP-tree的新能源汽车产业国际竞争力影响因素关联挖掘算法

为充分挖掘新能源汽车产业国际竞争力影响因素、探究价值增长点,提出了基于FP-tree的影响因素关联挖掘算法。通过构建国际竞争力各指标影响因素的关联规则,分析任意事务数据集中的关联数据,利用最小支持度参数minsup按照从上到下的方式搜索,确定最长的频繁项目集,采用FP-tree关联频繁项目集,设定分支关联性挖掘标准,实现了新能源汽车产业国际竞争力影响因素的挖掘。测试结果表明,设计算法的最小支持度和数据关联挖掘时间较短,置信度分析具有较高的稳定性。

Rising utilization of stable isotopes in tree rings for climate change and forest ecology

Analyses of stable isotopes(C,O,H)in tree rings are increasingly important cross-disciplinary programs.The rapid development in this field documented in an increasing number of publications requires a comprehensive review.This study includes a bibliometric analysis-based review to better understand research trends in tree ring stable isotope research.Overall,1475 publications were selected from the Web of Science Core Collection for 1974-2023.The findings are that:(1)numbers of annual publications and citations increased since 1974.From 1974 to 1980,there were around two relevant publications per year.However,from 2020 to 2022,this rose sharply to 109 publications per year.Likewise,average article citations were less than four per year before 1990,but were around four per article per year after 2000;(2)the major subjects using tree ring stable isotopes include forestry,geosciences,and environmental sciences,contributing to 42.5%of the total during 1974-2023;(3)the top three most productive institutions are the Chinese Academy of Sciences(423),the Swiss Federal Institute for Forest,Snow and Landscape Research(227),and the University of Arizona(204).These achievements result from strong collaborations;(4)review papers,for example,(Dawson et al.,Annu Rev Ecol Syst 33:507-559,2002)and(McCarroll and Loader,Quat Sci Rev 23:771-801,2004),are among the most cited,with more than 1000 citations;(5)tree ring stable isotope studies mainly focus on climatology and ecology,with atmospheric CO_(2) one of the most popular topics.Since 2010,precipitation and drought have received increasing attention.Based on this analysis,the research stages,key findings,debated issues,limitations and direc-tions for future research are summarized.This study serves as an important attempt to understand the progress on the use of stable isotopes in tree rings,providing scientific guid-ance for young researchers in this field.

Stress-assisted corrosion mechanism of 3Ni steel by using gradient boosting decision tree machining learning method

Traditional 3Ni weathering steel cannot completely meet the requirements for offshore engineering development,resulting in the design of novel 3Ni steel with the addition of microalloy elements such as Mn or Nb for strength enhancement becoming a trend.The stress-assisted corrosion behavior of a novel designed high-strength 3Ni steel was investigated in the current study using the corrosion big data method.The information on the corrosion process was recorded using the galvanic corrosion current monitoring method.The gradi-ent boosting decision tree(GBDT)machine learning method was used to mine the corrosion mechanism,and the importance of the struc-ture factor was investigated.Field exposure tests were conducted to verify the calculated results using the GBDT method.Results indic-ated that the GBDT method can be effectively used to study the influence of structural factors on the corrosion process of 3Ni steel.Dif-ferent mechanisms for the addition of Mn and Cu to the stress-assisted corrosion of 3Ni steel suggested that Mn and Cu have no obvious effect on the corrosion rate of non-stressed 3Ni steel during the early stage of corrosion.When the corrosion reached a stable state,the in-crease in Mn element content increased the corrosion rate of 3Ni steel,while Cu reduced this rate.In the presence of stress,the increase in Mn element content and Cu addition can inhibit the corrosion process.The corrosion law of outdoor-exposed 3Ni steel is consistent with the law based on corrosion big data technology,verifying the reliability of the big data evaluation method and data prediction model selection.

No effect of invasive tree species on aboveground biomass increments of oaks and pines in temperate forests

Prunus serotina and Robinia pseudoacacia are the most widespread invasive trees in Central Europe.In addition,according to climate models,decreased growth of many economically and ecologically important native trees will likely be observed in the future.We aimed to assess the impact of these two neophytes,which differ in the biomass range and nitrogen-fixing abilities observed in Central European conditions,on the relative aboveground biomass increments of native oaks Qucrcus robur and Q.petraea and Scots pine Pinus sylvestris.We aimed to increase our understanding of the relationship between facilitation and competition between woody alien species and overstory native trees.We established 72 circular plots(0.05 ha)in two different forest habitat types and stands varying in age in western Poland.We chose plots with different abundances of the studied neophytes to determine how effects scaled along the quantitative invasion gradient.Furthermore,we collected growth cores of the studied native species,and we calculated aboveground biomass increments at the tree and stand levels.Then,we used generalized linear mixed-effects models to assess the impact of invasive species abundances on relative aboveground biomass increments of native tree species.We did not find a biologically or statistically significant impact of invasive R.pseudoacacia or P.serotina on the relative aboveground,biomass increments of native oaks and pines along the quantitative gradient of invader biomass or on the proportion of total stand biomass accounted for by invaders.The neophytes did not act as native tree growth stimulators but also did not compete with them for resources,which would escalate the negative impact of climate change on pines and oaks.The neophytes should not significantly modify the carbon sequestration capacity of the native species.Our work combines elements of the per capita effect of invasion with research on mixed forest management.

Remaining Useful Life Prediction Method for Multi-Component System Considering Maintenance:Subsea Christmas Tree System as A Case Study

Maintenance is an important technical measure to maintain and restore the performance status of equipment and ensure the safety of the production process in industrial production,and is an indispensable part of prediction and health management.However,most of the existing remaining useful life(RUL)prediction methods assume that there is no maintenance or only perfect maintenance during the whole life cycle;thus,the predicted RUL value of the system is obviously lower than its actual operating value.The complex environment of the system further increases the difficulty of maintenance,and its maintenance nodes and maintenance degree are limited by the construction period and working conditions,which increases the difficulty of RUL prediction.An RUL prediction method for a multi-omponent system based on the Wiener process considering maintenance is proposed.The performance degradation model of components is established by a dynamic Bayesian network as the initial model,which solves the uncertainty of insufficient data problems.Based on the experience of experts,the degree of degradation is divided according to Poisson process simulation random failure,and different maintenance strategies are used to estimate a variety of condition maintenance factors.An example of a subsea tree system is given to verify the effectiveness of the proposed method.

Risk Assessment of Deep-Water Horizontal X-Tree Installation

Due to the high potential risk and many influencing factors of subsea horizontal X-tree installation,to guarantee the successful completion of sea trials of domestic subsea horizontal X-trees,this paper established a modular risk evaluation model based on a fuzzy fault tree.First,through the analysis of the main process oftree down and combining the Offshore&Onshore Reliability Data(OREDA)failure statistics and the operation procedure and the data provided by the job,the fault tree model of risk analysis of the tree down installation was established.Then,by introducing the natural language of expert comprehensive evaluation and combining fuzzy principles,quantitative analysis was carried out,and the fuzzy number was used to calculate the failure probability of a basic event and the occurrence probability of a top event.Finally,through a sensitivity analysis of basic events,the basic events of top events significantly affected were determined,and risk control and prevention measures for the corresponding high-risk factors were proposed for subsea horizontal X-tree down installation.

Identification of novel mammalian viruses in tree shrews(Tupaia belangeri chinensis)

The Chinese tree shrew(Tupaia belangeri chinensis),a member of the mammalian order Scandentia,exhibits considerable similarities with primates,including humans,in aspects of its nervous,immune,and metabolic systems.These similarities have established the tree shrew as a promising experimental model for biomedical research on cancer,infectious diseases,metabolic disorders,and mental health conditions.Herein,we used metatranscriptomic sequencing to analyze plasma,as well as oral and anal swab samples,from 105 healthy asymptomatic tree shrews to identify the presence of potential zoonotic viruses.In total,eight mammalian viruses with complete genomes were identified,belonging to six viral families,including Flaviviridae,Hepeviridae,Parvovirinae,Picornaviridae,Sedoreoviridae,and Spinareoviridae.Notably,the presence of rotavirus was recorded in tree shrews for the first time.Three viruses-hepacivirus 1,parvovirus,and picornavirus-exhibited low genetic similarity(<70%)with previously reported viruses at the whole-genome scale,indicating novelty.Conversely,three other viruses-hepacivirus 2,hepatovirus A and hepevirus-exhibited high similarity(>94%)to known viral strains.Phylogenetic analyses also revealed that the rotavirus and mammalian orthoreovirus identified in this study may be novel reassortants.These findings provide insights into the diverse viral spectrum present in captive Chinese tree shrews,highlighting the necessity for further research into their potential for crossspecies transmission.

基于TREE-LSTM算法的船舶汽轮机组变负荷故障诊断

针对船舶汽轮机组变负荷过程故障诊断中的耦合参数时序特征难以捕捉以及正常参数变动的干扰等问题,引入TREE-LSTM神经网络模型以实现复杂非线性系统动态数据分类。首先建立某船舶汽轮机组仿真模型,分析并进行故障仿真;随后进行数据预处理与特征工程;最后训练TREE-LSTM模型进行故障诊断,并与SVM、LSTM等模型进行比较。TREE-LSTM模型对于船舶汽轮机组变负荷过程的故障诊断正确率为98.7%,正确率最高。由于引入时间序列与复杂神经网络拓扑结构,TREE-LSTM在处理非线性系统动态数据分类问题时效果更好。

基于Extra Tree Classifier的水质安全建模预测

随着工业化和城市化的快速发展,水质安全问题日益受到关注。本研究利用一个包含7999条数据记录的水质分析数据集,涵盖多种化学物质浓度测量值与安全阈值,以及“是否安全”分类变量,运用Extr aTree Classifier模型进行水质安全建模预测及数据分析。本研究目的在于提供一个可靠的模型,以帮助决策者和相关部门更好地监测和维护水质安全,从而保障公众健康和环境可持续发展。

Short lifespan and ‘prime period’ of carbon sequestration call for multi-ages in dryland tree plantations

Enhancing forest cover is important for effective climate change mitigation.Studies suggest that drylands are promising areas for expanding forests,but conflicts arise with increased forest area and water consumption.Recent tree mortality in drylands raises concerns about carbon sequestration potential in tree plantations.Using Chinese dryland tree plantations as an example,we compared their growth with natural forests.Our results suggested plantation trees grew 1.6–2.1 times faster in juvenile phases,significantly shortening time to maturity(13.5 vs.30 years)compared to natural forests,potentially stemming from simple plantation age structures.Different from natural forests,74%of trees in plantations faced growth decline,indicating a short“prime period”for carbon sequestration and even a short lifespan.Additionally,a negative relationship between evapotranspiration and tree growth was observed in tree plantations since maturity,leading to high sensitivities of trees to vapor pressure deficit and soil water.However,this was not observed in natural forests.To address this,we suggest afforestation in drylands should consider complex age structures,ensuring a longer prime period for carbon sequestration and life expectancy in tree plantations.

Parkland trees on smallholder farms ameliorate soil physical-chemical properties in the semi-arid area of Tigray,Ethiopia

Proposed agroforestry options should begin with the species that farmers are most familiar with,which would be the native multipurpose trees that have evolved under smallholder farms and socioeconomic conditions.The African birch(Anogeissus leiocarpa(DC.)Guill.&Perr.)and pink jacaranda(Stereospermum kunthianum Cham.)trees are the dominant species in the agroforestry parkland system in the drylands of Tigray,Ethiopia.Smallholder farmers highly value these trees for their multifunctional uses including timber,firewood,charcoal,medicine,etc.These trees also could improve soil fertility.However,the amount of soil physical and chemical properties enhanced by the two species must be determined to maintain the sustainable conservation of the species in the parklands and to scale up to similar agroecological systems.Hence,we selected twelve isolated trees,six from each species that had similar dendrometric characteristics and were growing in similar environmental conditions.We divided the canopy cover of each tree into three radial distances:mid-canopy,canopy edge,and canopy gap(control).At each distance,we took soil samples from three different depths.We collected 216 soil samples(half disturbed and the other half undisturbed)from each canopy position and soil depth.Bulk density(BD),soil moisture content(SMC),soil organic carbon(SOC),total nitrogen(TN),available phosphorus(AP),available potassium(AK),p H,electrical conductivity(EC),and cation exchange capacity(CEC)were analysed.Results revealed that soil physical and chemical properties significantly improved except for soil texture and EC under both species,CEC under A.leiocarpus,and soil p H under S.kunthianum,all the studied soils were improved under both species canopy as compared with canopy gap.SMC,TN,AP,and AK under canopy of these trees were respectively 24.1%,11.1%,55.0%,and 9.3% higher than those soils under control.The two parkland agroforestry species significantly enhanced soil fertility near the canopy of topsoil through improving soil physical and chemical properties.These two species were recommended in the drylands with similar agro-ecological systems.

Tree mycorrhizal associations determine how biodiversity,large trees,and environmental factors drive aboveground carbon stock in temperate forests

Biodiversity,large trees,and environmental conditions such as climate and soil have important effects on forest carbon stocks.However,recent studies in temperate forests suggest that the relative importance of these factors depends on tree mycorrhizal associations,whereby large-tree effects may be driven by ectomycorrhizal(EM)trees,diversity effects may be driven by arbuscular mycorrhizal(AM)trees,and environment effects may depend on differential climate and soil preferences of AM and EM trees.To test this hypothesis,we used forest-inventory data consisting of over 80,000 trees from 631 temperate-forest plots(30 m×30 m)across Northeast China to examine how biodiversity(species diversity and ecological uniqueness),large trees(top 1%of tree diameters),and environmental factors(climate and soil nutrients)differently regulate aboveground carbon stocks of AM trees,EM trees,and AM and EM trees combined(i.e.total aboveground carbon stock).We found that large trees had a positive effect on both AM and EM tree carbon stocks.However,biodiversity and environmental factors had opposite effects on AM vs.EM tree carbon stocks.Specifically,the two components of biodiversity had positive effects on AM tree carbon stocks,but negative effects on EM tree carbon stocks.Environmental heterogeneity(mean annual temperature and soil nutrients)also exhibited contrasting effects on AM and EM tree carbon stocks.Consequently,for the total carbon stock,the positive large-tree effect far surpasses the diversity and environment effect.This is mainly because when integrating AM and EM tree carbon stock into total carbon stock,the opposite diversity-effect(also environment-effect)on AM vs.EM tree carbon stock counteracts each other while the consistent positive large-tree effect on AM and EM tree carbon stock is amplified.In summary,this study emphasized a mycorrhizal viewpoint to better understand the determinants of overarching aboveground carbon profile across regional forests.

Aging Mongolian pine plantations face high risks of drought-induced growth decline:evidence from both individual tree and forest stand measurements

Discerning vulnerability differences among different aged trees to drought-driven growth decline or to mortality is critical to implement age-specific countermeasures for forest management in water-limited areas.An important species for afforestation in dry environments of northern China,Mongolian pine(Pinus sylvestris var.mongolica Litv.)has recently exhibited growth decline and dieback on many sites,particularly pronounced in old-growth plantations.However,changes in response to drought stress by this species with age as well as the underlying mechanisms are poorly understood.In this study,tree-ring data and remotely sensed vegetation data were combined to investigate variations in growth at individual tree and stand scales for young(9-13 years)and aging(35-52 years)plantations of Mongolian pine in a water-limited area of northern China.A recent decline in tree-ring width in the older plantation also had lower values in satellited-derived normalized difference vegetation indices and normalized difference water indices relative to the younger plantations.In addition,all measured growth-related metrics were strongly correlated with the self-calibrating Palmer drought severity index during the growing season in the older plantation.Sensitivity of growth to drought of the older plantation might be attributed to more severe hydraulic limitations,as reflected by their lower sapwood-and leaf-specific hydraulic conductivities.Our study presents a comprehensive view on changes of growth with age by integrating multiple methods and provides an explanation from the perspective of plant hydraulics for growth decline with age.The results indicate that old-growth Mongolian pine plantations in water-limited environments may face increased growth declines under the context of climate warming and drying.

Long-Term Impacts of Tree Architectures and Branch Configurations on Tree Growth, Yield, Fruit Quality Attributes, and Leaf Minerals in “Aztec Fuji” Apple

Canopy and branch architectures in high-density orchards can be crucial in production and fruit quality. The influence of two canopy orientations (Upright and Tilted) in combination with two arm (branch) architectures (Shortened or Overlapped) on tree growth, yield components, fruit quality, and leaf mineral nutrients in an “Aztec Fuji” apple (Malus domestica Bork.) high-density orchard was studied over five years. Tilted trees with shortened arm configuration (TilShArm) always had significantly larger trunk cross-sectional area (TCSA) than Upright trees with an Overlapped arm configuration (UpOverArm) every year from 2012 to 2016. Trees with a TilShArm system had more cumulative fruit per tree than those with an Upright orientation. Trees with a tilted canopy (TilShArm and TilOverArm) tended to have higher yield per tree and yield per hectare than those with an upright system. Trees with a TilShArm system were more precocious and had more yield per tree than those with an upright canopy orientation in 2012. When values were polled over five years, trees with an upright canopy-shortened arm system (UpShArm) treatment had a lower biennial bearing index (BBI) than those with an upright canopy-overlapped system (UpOverArm). Trees receiving an arm shortening (UpShArm or TilShArm) configuration often had larger fruits than those with overlapped arms (UpOverArm and TilOverArm). Fruit from trees receiving an UpOverArm had higher fruit firmness than those from trees with other canopy-branch arrangements at harvest due to their smaller size. Fruit from trees with a TilShArm and TilOverArm had significantly higher water core and bitter pit but lower sunburn than trees with an upright canopy (UpShArm and UpOverArm). Leaves from trees with an UpOverArm canopy-branch configuration had the lowest leaf Ca but the highest leaf K and Fe concentrations among all treatments.

N-fixing tree species promote the chemical stability of soil organic carbon in subtropical plantations through increasing the relative contribution of plant-derived lipids

Biodiversity experiments have shown that soil organic carbon(SOC)is not only a function of plant diversity,but is also closely related to the nitrogen(N)-fixing plants.However,the effect of N-fixing trees on SOC chemical stability is still little known,especially with the compounding effects of tree species diversity.An experimental field manipulation was established in subtropical plantations of southern China to explore the impacts of tree species richness(i.e.,one,two,four and six tree species)and with/without N-fixing trees on SOC chemical stability,as indicated by the ratio of easily oxidized organic carbon to SOC(EOC/SOC).Plant-derived C components in terms of hydrolysable plant lipids and lignin phenols were isolated from soils for evaluating their relative contributions to SOC chemical stability.The results showed that N-fixing tree species rather than tree species richness had a significant effect on EOC/SOC.Hydrolysable plant lipids and lignin phenols were negatively correlated with EOC/SOC,while hydrolysable plant lipids contributed more to EOC/SOC than lignin phenols,especially in the occurrence of N-fixing trees.The presence of N-fixing tree species led to an increase in soil N availability and a decrease in fungal abundance,promoting the selective retention of certain key components of hydrolysable plant lipids,thus enhancing SOC chemical stability.These findings underpin the crucial role of N-fixing trees in shaping SOC chemical stability,and therefore,preferential selection of N-fixing tree species in mixed plantations is an appropriate silvicultural strategy to improve SOC chemical stability in subtropical plantations.

Improved Data Stream Clustering Method: Incorporating KD-Tree for Typicality and Eccentricity-Based Approach

Data stream clustering is integral to contemporary big data applications.However,addressing the ongoing influx of data streams efficiently and accurately remains a primary challenge in current research.This paper aims to elevate the efficiency and precision of data stream clustering,leveraging the TEDA(Typicality and Eccentricity Data Analysis)algorithm as a foundation,we introduce improvements by integrating a nearest neighbor search algorithm to enhance both the efficiency and accuracy of the algorithm.The original TEDA algorithm,grounded in the concept of“Typicality and Eccentricity Data Analytics”,represents an evolving and recursive method that requires no prior knowledge.While the algorithm autonomously creates and merges clusters as new data arrives,its efficiency is significantly hindered by the need to traverse all existing clusters upon the arrival of further data.This work presents the NS-TEDA(Neighbor Search Based Typicality and Eccentricity Data Analysis)algorithm by incorporating a KD-Tree(K-Dimensional Tree)algorithm integrated with the Scapegoat Tree.Upon arrival,this ensures that new data points interact solely with clusters in very close proximity.This significantly enhances algorithm efficiency while preventing a single data point from joining too many clusters and mitigating the merging of clusters with high overlap to some extent.We apply the NS-TEDA algorithm to several well-known datasets,comparing its performance with other data stream clustering algorithms and the original TEDA algorithm.The results demonstrate that the proposed algorithm achieves higher accuracy,and its runtime exhibits almost linear dependence on the volume of data,making it more suitable for large-scale data stream analysis research.

Decline in tree-ring growth of Picea mongolica and its intra-annual eco-physiological responses to drought and CO_(2) enrichment in semi-arid China

Episodes of drought-induced decline in tree growth and mortality are becoming more frequent as a result of climate warming and enhanced water stress in semi-arid areas.However,the ecophysiological mechanisms under-lying the impact of drought on tree growth remains unre-solved.In this study,earlywood and latewood tree-ring growth,δ^(13)C,andδ^(18)O chronologies of Picea mongolica from 1900 to 2013 were developed to clarify the intra-and inter-annual tree-ring growth responses to increasingly fre-quent droughts.The results indicate that annual basal area increment residuals(BAI_(res)),which removed tree age and size effects,have significantly decreased since 1960.How-ever,the decreasing trend of earlywood BAI_(res) was higher than that of latewood.Climate response analysis suggests that the dominant parameters for earlywood and latewood proxies(BAI_(res),δ^(13)C andδ^(18)O)were drought-related climate variables(Palmer drought severity index,temperature,rela-tive humidity,and vapor pressure deficit).The most signifi-cant period of earlywood and latewood proxies’responses to climate variables were focused on June-July and July-August,respectively.BAI_(res),andδ^(13)C were significantly affected by temperature and moisture conditions,whereasδ^(18)O was slightly affected.Decreasing stomatal conduct-ance due to drought outweighed the influence of increasing CO_(2) on intrinsic water use efficiency(iWUE),and ultimately led to a decline in BAI_(res).Compared to latewood,the faster decreasing BAI_(res) and smaller increasing iWUE of early-wood suggested trees were more vulnerable to water stress in the early growing season.Our study provides insights into the inter-and intra-annual mechanisms of tree-ring growth in semi-arid regions under rising CO_(2) and climate change.

Ecological network analysis reveals complex responses of tree species life stage interactions to stand variables

Tree interactions are essential for the structure,dynamics,and function of forest ecosystems,but variations in the architecture of life-stage interaction networks(LSINs)across forests is unclear.Here,we constructed 16 LSINs in the mountainous forests of northwest Hebei,China based on crown overlap from four mixed forests with two dominant tree species.Our results show that LSINs decrease the complexity of stand densities and basal areas due to the interaction cluster differentiation.In addition,we found that mature trees and saplings play different roles,the first acting as“hub”life stages with high connectivity and the second,as“bridges”controlling information flow with high centrality.Across the forests,life stages with higher importance showed better parameter stability within LSINs.These results reveal that the structure of tree interactions among life stages is highly related to stand variables.Our efforts contribute to the understanding of LSIN complexity and provide a basis for further research on tree interactions in complex forest communities.

Comprehensive analyses of the proteome and ubiquitome revealed mechanism of high temperature accelerating petal abscission in tree peony

Tree peony(Paeonia suffruticosa Andrews)is a well-known ornamental plant with high economic value,but the short fluorescence is a key obstacle to its ornamental value and industry development.High temperature accelerates flower senescence and abscission,but the associated mechanisms are poorly understood.In this study,the tandem mass tag(TMT)proteome and label-free quantitative ubiquitome from tree peony cut flowers treated with 20℃for 0 h(RT0),20℃or 28℃for 60 h(RT60 or HT60)were examined based on morphological observation,respectively.Totally,6970 proteins and 1545 lysine ubiquitinated(Kub)sites in 844 proteins were identified.Hydrophilic residues(such as glutamate and aspartate)neighboring the Kub sites were in preference,and 36.01%of the Kub sites were located on the protein surface.The differentially expressed proteins(DEPs)and Kub-DEPs in HT60 vs RT60 were mainly enriched in ribosomal protein,protein biosynthesis,secondary metabolites biosynthesis,flavonoid metabolism,carbohydrate catabolism,and auxin biosynthesis and signaling revealed by GO and KEGG analysis,accompanying the increase of endogenous abscisic acid(ABA)accumulation and decrease of endogenous indoleacetic acid(IAA)level.Additionally,the expression patterns of six enzymes(SAMS,ACO,YUC,CHS,ANS and PFK)putatively with Kub modifications were analyzed by proteome and real-time quantitative RT-PCR.The cell-free degradation assays showed PsSAMS and PsACO proteins could be degraded via the 26 S proteasome system in tree peony flowers.Finally,a working model was proposed for the acceleration of flower senescence and abscission by high temperature.In summary,all results contributed to understanding the mechanism of flower senescence induced by high temperature and prolonging fluorescence in tree peony.