Wilms肿瘤易感基因(Wilms tumor type 1,WT1)起初作为肿瘤抑制基因而被大家认识,而最近报道指出WT1作为原癌基因在正常造血系统细胞增殖分化过程中发挥着重要的作用,且预示着较差的肿瘤预后。表明WT1基因与疾病的发生、发展及预后存在...Wilms肿瘤易感基因(Wilms tumor type 1,WT1)起初作为肿瘤抑制基因而被大家认识,而最近报道指出WT1作为原癌基因在正常造血系统细胞增殖分化过程中发挥着重要的作用,且预示着较差的肿瘤预后。表明WT1基因与疾病的发生、发展及预后存在密切关系。近年来对WT1基因的表达、突变与急性髓系白血病(acute myeloid leukemia,AML)疗效和预后的关系有进一步的研究进展。本文就近几年WT1在AML疾病中的作用进行了综述,旨在全面考虑该基因对于疾病的作用并对患者实施分层治疗,从而达到精准治疗。展开更多
Objectives. The identification of proteins that are selectively expressed in cancer and with potential to elicit an immune response is the first step towards antigen- specific immunotherapy. The Wilms tumor gene produ...Objectives. The identification of proteins that are selectively expressed in cancer and with potential to elicit an immune response is the first step towards antigen- specific immunotherapy. The Wilms tumor gene product (WT1) is inherently immunogenic and is now thought to be oncogenic. The aim of this study was to determine the expression of WT1 in epithelial ovarian cancer (EOC) and correlate with clinico- pathologic characteristics. Methods. WT1 expression was examined using immunohistochemistry applied on a tissue microarray of normal tissues and a panel of 100 EOC tissues. The distribution of WT1 expression and clinico- pathologic variables were analyzed. Survival probabilities were estimated by Kaplan- Meier method, and statistical significance was determined by the logrank test. Results. WT1 expression was observed in 78/100 of specimens. The predominant expression pattern was homogenous, occurring in 66/100 (66% ) of WT1- positive specimens, while 12/100 (12% ) demonstrated heterogeneous staining. In normal tissues, WT1 expression was noted in kidneys, splenic capsule, Sertoli cells of the testis, and granulosa cells of the ovary. The median follow- up of the patient population was 30 months. Patients with WT1- positive tumors tended to have a higher grade (P = 0.006) and stage (P = 0.002) of tumor. However, there were no significant differences in the distribution of patients with WT1- positive tumors in relation to disease- free and overall survival. Conclusions. Our data demonstrate that WT1 is expressed at high frequency in patients with EOC. Since WT1 demonstrates tissue- restricted expression and is inherently immunogenic, it could represent an attractive target for antigen- specific immunotherapy in EOC.展开更多
目的:研究胰腺导管腺癌组织中WT1,IGF-IR的表达与细胞凋亡关系．方法:应用免疫组化技术检测WT1,IGF-IR在49例胰腺导管腺癌及15例正常胰腺组织中的表达,并应用TUNEL法检测细胞凋亡,计算凋亡指数(AI)．结果:WT1,IGF-IR在正常胰腺组织中的...目的:研究胰腺导管腺癌组织中WT1,IGF-IR的表达与细胞凋亡关系．方法:应用免疫组化技术检测WT1,IGF-IR在49例胰腺导管腺癌及15例正常胰腺组织中的表达,并应用TUNEL法检测细胞凋亡,计算凋亡指数(AI)．结果:WT1,IGF-IR在正常胰腺组织中的阳性表达率分别为26．67％(4／15)、40．00％(6／15);在胰腺导管腺癌组织中的阳性表达率分别为71．43％(35／49)、77．55％(38／49),两者在癌组织中的表达分别明显高于其在正常胰腺组织中的表达(P<0．05),且在癌组织中的表达呈正相关(r=0．385,P<0．05)．正常胰腺组织及癌组织中的AI分别为0．41±0．13、5．93±4．18,两者比较有显著性差异(P<0．05),癌组织中AI随组织分化程度的升高而升高．IGF-IR表达阳性组的AI显著低于阴性组(4．11±3．68 vs 12．21±5．67,P<0．01)．结论:胰腺导管腺癌组织中IGF-IR的高表达抑制细胞凋亡,WT1,IGF-IR的高表达以及细胞凋亡的减少可能在胰腺导管腺癌的发生发展中起重要作用．展开更多
文摘Objectives. The identification of proteins that are selectively expressed in cancer and with potential to elicit an immune response is the first step towards antigen- specific immunotherapy. The Wilms tumor gene product (WT1) is inherently immunogenic and is now thought to be oncogenic. The aim of this study was to determine the expression of WT1 in epithelial ovarian cancer (EOC) and correlate with clinico- pathologic characteristics. Methods. WT1 expression was examined using immunohistochemistry applied on a tissue microarray of normal tissues and a panel of 100 EOC tissues. The distribution of WT1 expression and clinico- pathologic variables were analyzed. Survival probabilities were estimated by Kaplan- Meier method, and statistical significance was determined by the logrank test. Results. WT1 expression was observed in 78/100 of specimens. The predominant expression pattern was homogenous, occurring in 66/100 (66% ) of WT1- positive specimens, while 12/100 (12% ) demonstrated heterogeneous staining. In normal tissues, WT1 expression was noted in kidneys, splenic capsule, Sertoli cells of the testis, and granulosa cells of the ovary. The median follow- up of the patient population was 30 months. Patients with WT1- positive tumors tended to have a higher grade (P = 0.006) and stage (P = 0.002) of tumor. However, there were no significant differences in the distribution of patients with WT1- positive tumors in relation to disease- free and overall survival. Conclusions. Our data demonstrate that WT1 is expressed at high frequency in patients with EOC. Since WT1 demonstrates tissue- restricted expression and is inherently immunogenic, it could represent an attractive target for antigen- specific immunotherapy in EOC.
文摘目的:研究胰腺导管腺癌组织中WT1,IGF-IR的表达与细胞凋亡关系．方法:应用免疫组化技术检测WT1,IGF-IR在49例胰腺导管腺癌及15例正常胰腺组织中的表达,并应用TUNEL法检测细胞凋亡,计算凋亡指数(AI)．结果:WT1,IGF-IR在正常胰腺组织中的阳性表达率分别为26．67％(4／15)、40．00％(6／15);在胰腺导管腺癌组织中的阳性表达率分别为71．43％(35／49)、77．55％(38／49),两者在癌组织中的表达分别明显高于其在正常胰腺组织中的表达(P<0．05),且在癌组织中的表达呈正相关(r=0．385,P<0．05)．正常胰腺组织及癌组织中的AI分别为0．41±0．13、5．93±4．18,两者比较有显著性差异(P<0．05),癌组织中AI随组织分化程度的升高而升高．IGF-IR表达阳性组的AI显著低于阴性组(4．11±3．68 vs 12．21±5．67,P<0．01)．结论:胰腺导管腺癌组织中IGF-IR的高表达抑制细胞凋亡,WT1,IGF-IR的高表达以及细胞凋亡的减少可能在胰腺导管腺癌的发生发展中起重要作用．
