The traditional deterministic analysis for tunnel face stability neglects the uncertainties of geotechnical parameters,while the simplified reliability analysis which models the potential uncertainties by means of ran...The traditional deterministic analysis for tunnel face stability neglects the uncertainties of geotechnical parameters,while the simplified reliability analysis which models the potential uncertainties by means of random variables usually fails to account for soil spatial variability.To overcome these limitations,this study proposes an efficient framework for conducting reliability analysis and reliability-based design(RBD)of tunnel face stability in spatially variable soil strata.The three-dimensional(3D)rotational failure mechanism of the tunnel face is extended to account for the soil spatial variability,and a probabilistic framework is established by coupling the extended mechanism with the improved Hasofer-Lind-Rackwits-Fiessler recursive algorithm(iHLRF)as well as its inverse analysis formulation.The proposed framework allows for rapid and precise reliability analysis and RBD of tunnel face stability.To demonstrate the feasibility and efficacy of the proposed framework,an illustrative case of tunnelling in frictional soils is presented,where the soil's cohesion and friction angle are modelled as two anisotropic cross-correlated lognormal random fields.The results show that the proposed method can accurately estimate the failure probability(or reliability index)regarding the tunnel face stability and can efficiently determine the required supporting pressure for a target reliability index with soil spatial variability being taken into account.Furthermore,this study reveals the impact of various factors on the support pressure,including coefficient of variation,cross-correlation between cohesion and friction angle,as well as autocorrelation distance of spatially variable soil strata.The results also demonstrate the feasibility of using the forward and/or inverse first-order reliability method(FORM)in high-dimensional stochastic problems.It is hoped that this study may provide a practical and reliable framework for determining the stability of tunnels in complex soil strata.展开更多
Online reactivity monitoring plays an important role in operation and safety analyses of fission reactor systems. The inverse kinetics method, which is based on a point kinetics model, is the most widely used method f...Online reactivity monitoring plays an important role in operation and safety analyses of fission reactor systems. The inverse kinetics method, which is based on a point kinetics model, is the most widely used method for reactivity reconstruction of critical water reactors. However, this method is seldom applied to the reactivity reconstruction of subcritical reactors. In this study, an inverse kinetics method was employed for the reactivity reconstruction of a lead-based reactor under different initial reactivity states(ρ_0= 0,-2786,-5486,-8367, and-12,371 pcm). The results showed that the deviation in the reactivity of the lead-based subcritical reactor was greater when ρ_0 became smaller. The reactivity reconstructed using the inverse kinetics method was globally underestimated. At a given reactivity perturbation, the relative and absolute errors increased with the decrease in the initial reactivity. At a given initial reactivity, with the increase in the reactivity perturbation, the absolute error increased, whereas the relative error remained the same.This deviation is due to the variation in the external neutron source, spatial-spectral effects, and sub-diffusive effects, which require further study.展开更多
The paper presents a new solution of inverse displacement analysis of the general six degree-of-freedom serial robot.The inverse displacement analysis of the general serial robot is transformed into a minimization pro...The paper presents a new solution of inverse displacement analysis of the general six degree-of-freedom serial robot.The inverse displacement analysis of the general serial robot is transformed into a minimization problem and then the optimization method is adopted to solve the nonlinear least squares problem with the analytic form of new Jacobian matrix.In this way,joint variables of the general serial robot can be searched out quickly under the desired precision when positions of the three non-collinear end effector points are given.Compared with the general Newton iterative method,the proposed algorithm can search out the solution when the robot is at the singular configuration and the initial configuration used in the optimization method may also be the singular configuration.So the convergence domain is bigger than that of the general Newton iterative method.Another advantage of the proposed algorithm is that positions of the three non-collinear end effector points are usually much easier to be measured than the orientation of the end effector.The inverse displacement analysis of the general 6R(six-revolute-joint) serial robot is illustrated as an example and the simulation results verify the efficiency of the proposed algorithm.Because the three non-collinear points can be selected at random,the method can be applied to any other types of serial robots.展开更多
This paper proposes a sensitivity analysis method for engineering parameters using interval analyses.This method substantially extends the application of interval analysis method.In this scheme,parameter intervals and...This paper proposes a sensitivity analysis method for engineering parameters using interval analyses.This method substantially extends the application of interval analysis method.In this scheme,parameter intervals and decision-making target intervals are determined using the interval analysis method.As an example,an inverse analysis method for uncertainty is presented.The intervals of unknown parameters can be obtained by sampling measured data.Even for limited measured data,robust results can also be obtained with the inverse analysis method,which can be intuitively evaluated by the uncertainty expressed in terms of an interval.For complex nonlinear problems,an iteratively optimized inverse analysis model is proposed.In a given set of loose parameter intervals,all the unknown parameter intervals that satisfy the measured information can be obtained by an iteratively optimized inverse analysis model.The influences of measured precisions and the number of parameters on the results of the inverse analysis are evaluated.Finally,the uniqueness of the interval inverse analysis method is discussed.展开更多
In the past decade,notable progress has been achieved in the development of the generalized finite difference method(GFDM).The underlying principle of GFDM involves dividing the domain into multiple sub-domains.Within...In the past decade,notable progress has been achieved in the development of the generalized finite difference method(GFDM).The underlying principle of GFDM involves dividing the domain into multiple sub-domains.Within each sub-domain,explicit formulas for the necessary partial derivatives of the partial differential equations(PDEs)can be obtained through the application of Taylor series expansion and moving-least square approximation methods.Consequently,the method generates a sparse coefficient matrix,exhibiting a banded structure,making it highly advantageous for large-scale engineering computations.In this study,we present the application of the GFDM to numerically solve inverse Cauchy problems in two-and three-dimensional piezoelectric structures.Through our preliminary numerical experiments,we demonstrate that the proposed GFDMapproach shows great promise for accurately simulating coupled electroelastic equations in inverse problems,even with 3%errors added to the input data.展开更多
An inverse analysis procedure has been developed to interpret collected pore pressure data and observations during backward erosion piping(BEP)initiation and progression in sandy soils.The procedure has been applied t...An inverse analysis procedure has been developed to interpret collected pore pressure data and observations during backward erosion piping(BEP)initiation and progression in sandy soils.The procedure has been applied to laboratory models designed to mimic the initiation and progression of BEP through a constricted vertical outlet.The inverse analysis uses three-dimensional(3D)finite element method(FEM)to successively produce models of the hydraulic head regime surrounding progressive stages of BEP based on observations at the sample surface and pore pressure measurements obtained from the laboratory models.The inverse analysis results in a series of 3D contour plots that represent the hydraulic-head regime at each stage of the BEP development,allowing for assessing the development of BEP mechanism as well as calculating the critical hydraulic conditions required for various BEP stages to initiate and progress.Interpretation of the results identified four significant stages of the piping process:(1)loosened zone initiation,(2)channel initiation and progression,(3)riser sand fluidization,and(4)loosened zone progression.Interpretation of the hydraulic head contour plots allows assessment of the critical hydraulic gradients needed to initiate and progress various components of the BEP development.展开更多
Explicit Exact and Approximate Inverse Preconditioners for solving complex linear systems are introduced. A class of general iterative methods of second order is presented and the selection of iterative parameters is ...Explicit Exact and Approximate Inverse Preconditioners for solving complex linear systems are introduced. A class of general iterative methods of second order is presented and the selection of iterative parameters is discussed. The second order iterative methods behave quite similar to first order methods and the development of efficient preconditioners for solving the original linear system is a decisive factor for making the second order iterative methods superior to the first order iterative methods. Adaptive preconditioned Conjugate Gradient methods using explicit approximate preconditioners for solving efficiently large sparse systems of algebraic equations are also presented. The generalized Approximate Inverse Matrix techniques can be efficiently used in conjunction with explicit iterative schemes leading to effective composite semi-direct solution methods for solving large linear systems of algebraic equations.展开更多
The generalized method of variational analysis (GMVA) suggested for 2-D wind observations by Huang et al. is extended to 3-D cases. Just as in 2-D cases, the regularization idea is applied. But due to the complexity...The generalized method of variational analysis (GMVA) suggested for 2-D wind observations by Huang et al. is extended to 3-D cases. Just as in 2-D cases, the regularization idea is applied. But due to the complexity of the 3-D cases, the vertical vorticity is taken as a stable functional. The results indicate that wind observations can be both variationally optimized and ?ltered. The e?ciency of GMVA is also checked in a numerical test. Finally, 3-D wind observations with random disturbances are manipulated by GMVA after being ?ltered.展开更多
This paper presents an improved precise integration algorithm fortransient analysis of heat transfer and some other problems. Theoriginal precise integration method is improved by means of the inve-rse accuracy analys...This paper presents an improved precise integration algorithm fortransient analysis of heat transfer and some other problems. Theoriginal precise integration method is improved by means of the inve-rse accuracy analysis so that the parameter N, which has been takenas a constant and an independent pa- rameter without consideration ofthe problems in the original method, can be generated automaticallyby the algorithm itself.展开更多
Rock rebound relaxation deformation,or even rock burst,caused by the excavation of dam base and abutment or high rock slope affects their stability and results in the fall of mechanical properties of the rock.So an in...Rock rebound relaxation deformation,or even rock burst,caused by the excavation of dam base and abutment or high rock slope affects their stability and results in the fall of mechanical properties of the rock.So an inverse analysis method was proposed in this paper to establish the time-dependent model of deformation modulus caused by excavation rebound.The basic principle is based on the combination of observed data of the excavation rebound deformation of dam abutment or rock slope,and the calculated rebound deformation by FEM under ground stress at the corresponding time in the excavation process.The norm of the residuals of observed data and calculated data are taken as the objective function.Accordingly,the time-dependent model of bedrock deformation modulus can be established.The method displays its significance in the design of excavation,construction and operation management of dam base and high slope.展开更多
A simple data assimilation method for improving estimation of moderate resolution imaging spectroradiometer (MODIS) leaf area index (LAI) time-series data products based on the gradient inverse weighted filter and...A simple data assimilation method for improving estimation of moderate resolution imaging spectroradiometer (MODIS) leaf area index (LAI) time-series data products based on the gradient inverse weighted filter and object analysis is proposed. The properties and quality control (QC) of MODIS LAI data products are introduced. Also, the gradient inverse weighted filter and object analysis are analyzed. An experiment based on the simple data assimilation method is performed using MODIS LAI data sets from 2000 to 2005 of Guizhou Province in China.展开更多
In order to explore the nonlinear structure hidden in high-dimensional data, some dimen-sion reduction techniques have been developed, such as the Projection Pursuit technique (PP).However, PP will involve enormous co...In order to explore the nonlinear structure hidden in high-dimensional data, some dimen-sion reduction techniques have been developed, such as the Projection Pursuit technique (PP).However, PP will involve enormous computational load. To overcome this, an inverse regressionmethod is proposed. In this paper, we discuss and develop this method. To seek the interestingprojective direction, the minimization of the residual sum of squares is used as a criterion, andspline functions are applied to approximate the general nonlinear transform function. The algo-rithm is simple, and saves the computational load. Under certain proper conditions, consistencyof the estimators of the interesting direction is shown.展开更多
背景:膝骨关节炎是一种常见的关节软骨及周围组织损伤的慢性炎症性疾病,而免疫细胞在膝骨关节炎免疫炎症反应中起到重要作用,但其中的具体机制仍有待深入研究。目的:采用孟德尔随机化方法来评估731种免疫细胞表型与膝骨关节炎风险之间...背景:膝骨关节炎是一种常见的关节软骨及周围组织损伤的慢性炎症性疾病,而免疫细胞在膝骨关节炎免疫炎症反应中起到重要作用,但其中的具体机制仍有待深入研究。目的:采用孟德尔随机化方法来评估731种免疫细胞表型与膝骨关节炎风险之间的潜在因果关系。方法:使用全基因组关联分析(GWAS)目录中公开获取731种免疫细胞表型的全基因组关联分析统计数据(从GCST0001391到GCST0002121)和IEUGWAS数据库中膝骨关节炎的全基因组关联分析数据(ebi-a-GCST007090)。采用逆方差加权法、MR-Egger回归法、加权中位数法、加权模型法和简单模型法来研究免疫细胞与膝骨关节炎之间的因果关系。敏感性分析用于检验孟德尔随机化分析结果是否可靠,然后以同样方法进行反向孟德尔随机化分析。结果与结论:①正向分析结果表明,共有4种免疫细胞表型与膝骨关节炎有显著的因果关系(FDR<0.20),其中B细胞中的CD27 on CD24+CD27+(OR=1.026,P=0.00026,Pfdr=0.18)、髓系细胞中的CD33 on CD33dim HLA DR-(OR=1.014,P=0.00050,Pfdr=0.18)以及Treg细胞中的CD45RA+CD28-CD8br%CD8br(OR=1.001,P=0.00078,Pfdr=0.18)与膝骨关节炎风险呈直接的正向因果关联;单核细胞中PDL-1 on monocyte(OR=0.952,P=0.00098,Pfdr=0.18)与膝骨关节炎风险呈直接的负向因果关联。②反向分析结果表明,当膝骨关节炎作为暴露数据时,与731种免疫细胞表型均不具有显著因果关系(FDR<0.20)。③敏感性分析结果显示:双向孟德尔随机化的Cochran’s Q检验和MR-Egger回归法结果P值均大于0.05,表明免疫细胞表型与膝骨关节炎之间的因果效应分析不存在显著的异质性和多效性。④上述结果证实,CD27 on CD24+CD27+,CD33 on CD33dim HLA DR-,CD45RA+CD28-CD8br%CD8br以及PDL-1 on monocyte免疫细胞表型与膝骨关节炎之间可能具有较为显著的潜在因果关系,这为研究膝骨关节炎的生物学机制及探索膝骨关节炎的早期防治提供有价值的线索,也为干预性药物的开发提供了新的方向。展开更多
基金supported by the National Natural Science Foundation of China(Grant No.U22A20594)the Fundamental Research Funds for the Central Universities(Grant No.B230205028)the Postgraduate Research&Practice Innovation Program of Jiangsu Province(Grant No.KYCX23_0694).
文摘The traditional deterministic analysis for tunnel face stability neglects the uncertainties of geotechnical parameters,while the simplified reliability analysis which models the potential uncertainties by means of random variables usually fails to account for soil spatial variability.To overcome these limitations,this study proposes an efficient framework for conducting reliability analysis and reliability-based design(RBD)of tunnel face stability in spatially variable soil strata.The three-dimensional(3D)rotational failure mechanism of the tunnel face is extended to account for the soil spatial variability,and a probabilistic framework is established by coupling the extended mechanism with the improved Hasofer-Lind-Rackwits-Fiessler recursive algorithm(iHLRF)as well as its inverse analysis formulation.The proposed framework allows for rapid and precise reliability analysis and RBD of tunnel face stability.To demonstrate the feasibility and efficacy of the proposed framework,an illustrative case of tunnelling in frictional soils is presented,where the soil's cohesion and friction angle are modelled as two anisotropic cross-correlated lognormal random fields.The results show that the proposed method can accurately estimate the failure probability(or reliability index)regarding the tunnel face stability and can efficiently determine the required supporting pressure for a target reliability index with soil spatial variability being taken into account.Furthermore,this study reveals the impact of various factors on the support pressure,including coefficient of variation,cross-correlation between cohesion and friction angle,as well as autocorrelation distance of spatially variable soil strata.The results also demonstrate the feasibility of using the forward and/or inverse first-order reliability method(FORM)in high-dimensional stochastic problems.It is hoped that this study may provide a practical and reliable framework for determining the stability of tunnels in complex soil strata.
基金supported by the Strategic Priority Science and Technology Program of the Chinese Academy of Sciences(No.XDA03040000)the National Natural Science Foundation of China(NSFC)(Nos.11305205,11305203,and 11405204)+3 种基金the Special Program for Informatization of the Chinese Academy of Sciences(No.XXH12504-1-09)the Anhui Provincial Special project for High Technology Industrythe Special Project of Youth Innovation Promotion Association of Chinese Academy of Sciencesthe Industrialization Fund
文摘Online reactivity monitoring plays an important role in operation and safety analyses of fission reactor systems. The inverse kinetics method, which is based on a point kinetics model, is the most widely used method for reactivity reconstruction of critical water reactors. However, this method is seldom applied to the reactivity reconstruction of subcritical reactors. In this study, an inverse kinetics method was employed for the reactivity reconstruction of a lead-based reactor under different initial reactivity states(ρ_0= 0,-2786,-5486,-8367, and-12,371 pcm). The results showed that the deviation in the reactivity of the lead-based subcritical reactor was greater when ρ_0 became smaller. The reactivity reconstructed using the inverse kinetics method was globally underestimated. At a given reactivity perturbation, the relative and absolute errors increased with the decrease in the initial reactivity. At a given initial reactivity, with the increase in the reactivity perturbation, the absolute error increased, whereas the relative error remained the same.This deviation is due to the variation in the external neutron source, spatial-spectral effects, and sub-diffusive effects, which require further study.
基金Funded by National Natural Science Foundation of China (No. 50905102)the Natural Science Foundation of Guangdong Province (Nos. 10151503101000033 and 8351503101000001)the Building Fund for the Academic Innovation Team of Shantou University (No. ITC10003)
文摘The paper presents a new solution of inverse displacement analysis of the general six degree-of-freedom serial robot.The inverse displacement analysis of the general serial robot is transformed into a minimization problem and then the optimization method is adopted to solve the nonlinear least squares problem with the analytic form of new Jacobian matrix.In this way,joint variables of the general serial robot can be searched out quickly under the desired precision when positions of the three non-collinear end effector points are given.Compared with the general Newton iterative method,the proposed algorithm can search out the solution when the robot is at the singular configuration and the initial configuration used in the optimization method may also be the singular configuration.So the convergence domain is bigger than that of the general Newton iterative method.Another advantage of the proposed algorithm is that positions of the three non-collinear end effector points are usually much easier to be measured than the orientation of the end effector.The inverse displacement analysis of the general 6R(six-revolute-joint) serial robot is illustrated as an example and the simulation results verify the efficiency of the proposed algorithm.Because the three non-collinear points can be selected at random,the method can be applied to any other types of serial robots.
基金Supported by the National Natural Science Foundation of China(50978083)the Fundamental Research Funds for the Central Universities(2010B02814)
文摘This paper proposes a sensitivity analysis method for engineering parameters using interval analyses.This method substantially extends the application of interval analysis method.In this scheme,parameter intervals and decision-making target intervals are determined using the interval analysis method.As an example,an inverse analysis method for uncertainty is presented.The intervals of unknown parameters can be obtained by sampling measured data.Even for limited measured data,robust results can also be obtained with the inverse analysis method,which can be intuitively evaluated by the uncertainty expressed in terms of an interval.For complex nonlinear problems,an iteratively optimized inverse analysis model is proposed.In a given set of loose parameter intervals,all the unknown parameter intervals that satisfy the measured information can be obtained by an iteratively optimized inverse analysis model.The influences of measured precisions and the number of parameters on the results of the inverse analysis are evaluated.Finally,the uniqueness of the interval inverse analysis method is discussed.
基金the Natural Science Foundation of Shandong Province of China(Grant No.ZR2022YQ06)the Development Plan of Youth Innovation Team in Colleges and Universities of Shandong Province(Grant No.2022KJ140)the Key Laboratory ofRoad Construction Technology and Equipment(Chang’an University,No.300102253502).
文摘In the past decade,notable progress has been achieved in the development of the generalized finite difference method(GFDM).The underlying principle of GFDM involves dividing the domain into multiple sub-domains.Within each sub-domain,explicit formulas for the necessary partial derivatives of the partial differential equations(PDEs)can be obtained through the application of Taylor series expansion and moving-least square approximation methods.Consequently,the method generates a sparse coefficient matrix,exhibiting a banded structure,making it highly advantageous for large-scale engineering computations.In this study,we present the application of the GFDM to numerically solve inverse Cauchy problems in two-and three-dimensional piezoelectric structures.Through our preliminary numerical experiments,we demonstrate that the proposed GFDMapproach shows great promise for accurately simulating coupled electroelastic equations in inverse problems,even with 3%errors added to the input data.
基金support from the South China University of Technology for the PhD short-term visiting project。
文摘An inverse analysis procedure has been developed to interpret collected pore pressure data and observations during backward erosion piping(BEP)initiation and progression in sandy soils.The procedure has been applied to laboratory models designed to mimic the initiation and progression of BEP through a constricted vertical outlet.The inverse analysis uses three-dimensional(3D)finite element method(FEM)to successively produce models of the hydraulic head regime surrounding progressive stages of BEP based on observations at the sample surface and pore pressure measurements obtained from the laboratory models.The inverse analysis results in a series of 3D contour plots that represent the hydraulic-head regime at each stage of the BEP development,allowing for assessing the development of BEP mechanism as well as calculating the critical hydraulic conditions required for various BEP stages to initiate and progress.Interpretation of the results identified four significant stages of the piping process:(1)loosened zone initiation,(2)channel initiation and progression,(3)riser sand fluidization,and(4)loosened zone progression.Interpretation of the hydraulic head contour plots allows assessment of the critical hydraulic gradients needed to initiate and progress various components of the BEP development.
文摘Explicit Exact and Approximate Inverse Preconditioners for solving complex linear systems are introduced. A class of general iterative methods of second order is presented and the selection of iterative parameters is discussed. The second order iterative methods behave quite similar to first order methods and the development of efficient preconditioners for solving the original linear system is a decisive factor for making the second order iterative methods superior to the first order iterative methods. Adaptive preconditioned Conjugate Gradient methods using explicit approximate preconditioners for solving efficiently large sparse systems of algebraic equations are also presented. The generalized Approximate Inverse Matrix techniques can be efficiently used in conjunction with explicit iterative schemes leading to effective composite semi-direct solution methods for solving large linear systems of algebraic equations.
基金the National Natural Science Foundation of China(No.40075014,40175014)Shanghai Science and Technology Association(No.02DJ14032).
文摘The generalized method of variational analysis (GMVA) suggested for 2-D wind observations by Huang et al. is extended to 3-D cases. Just as in 2-D cases, the regularization idea is applied. But due to the complexity of the 3-D cases, the vertical vorticity is taken as a stable functional. The results indicate that wind observations can be both variationally optimized and ?ltered. The e?ciency of GMVA is also checked in a numerical test. Finally, 3-D wind observations with random disturbances are manipulated by GMVA after being ?ltered.
基金the National Natural Science Foundation of China (No.19872016,19872017)the National Key Basic Research Special Foundation (G1999032805)the Foundation for University Key Teachers by the Ministry of Education of China
文摘This paper presents an improved precise integration algorithm fortransient analysis of heat transfer and some other problems. Theoriginal precise integration method is improved by means of the inve-rse accuracy analysis so that the parameter N, which has been takenas a constant and an independent pa- rameter without consideration ofthe problems in the original method, can be generated automaticallyby the algorithm itself.
基金Supported by the National Natural Science Foundation of China (Grant Nos.50539110, 50809025,50539010,50579010,50539030)the National Science and Technology Supporting Plan (Grant Nos.20006BAC14B03,2008BAB29B06,2008BAB29B03)
文摘Rock rebound relaxation deformation,or even rock burst,caused by the excavation of dam base and abutment or high rock slope affects their stability and results in the fall of mechanical properties of the rock.So an inverse analysis method was proposed in this paper to establish the time-dependent model of deformation modulus caused by excavation rebound.The basic principle is based on the combination of observed data of the excavation rebound deformation of dam abutment or rock slope,and the calculated rebound deformation by FEM under ground stress at the corresponding time in the excavation process.The norm of the residuals of observed data and calculated data are taken as the objective function.Accordingly,the time-dependent model of bedrock deformation modulus can be established.The method displays its significance in the design of excavation,construction and operation management of dam base and high slope.
基金This work was supported by the China Postdoctoral Science Foundation(No.20060390326)the key international S&T cooperation project of China(No.2004DFA06300).
文摘A simple data assimilation method for improving estimation of moderate resolution imaging spectroradiometer (MODIS) leaf area index (LAI) time-series data products based on the gradient inverse weighted filter and object analysis is proposed. The properties and quality control (QC) of MODIS LAI data products are introduced. Also, the gradient inverse weighted filter and object analysis are analyzed. An experiment based on the simple data assimilation method is performed using MODIS LAI data sets from 2000 to 2005 of Guizhou Province in China.
基金This project is supported by the National Natural Science Foundation of China
文摘In order to explore the nonlinear structure hidden in high-dimensional data, some dimen-sion reduction techniques have been developed, such as the Projection Pursuit technique (PP).However, PP will involve enormous computational load. To overcome this, an inverse regressionmethod is proposed. In this paper, we discuss and develop this method. To seek the interestingprojective direction, the minimization of the residual sum of squares is used as a criterion, andspline functions are applied to approximate the general nonlinear transform function. The algo-rithm is simple, and saves the computational load. Under certain proper conditions, consistencyof the estimators of the interesting direction is shown.
文摘背景:膝骨关节炎是一种常见的关节软骨及周围组织损伤的慢性炎症性疾病,而免疫细胞在膝骨关节炎免疫炎症反应中起到重要作用,但其中的具体机制仍有待深入研究。目的:采用孟德尔随机化方法来评估731种免疫细胞表型与膝骨关节炎风险之间的潜在因果关系。方法:使用全基因组关联分析(GWAS)目录中公开获取731种免疫细胞表型的全基因组关联分析统计数据(从GCST0001391到GCST0002121)和IEUGWAS数据库中膝骨关节炎的全基因组关联分析数据(ebi-a-GCST007090)。采用逆方差加权法、MR-Egger回归法、加权中位数法、加权模型法和简单模型法来研究免疫细胞与膝骨关节炎之间的因果关系。敏感性分析用于检验孟德尔随机化分析结果是否可靠,然后以同样方法进行反向孟德尔随机化分析。结果与结论:①正向分析结果表明,共有4种免疫细胞表型与膝骨关节炎有显著的因果关系(FDR<0.20),其中B细胞中的CD27 on CD24+CD27+(OR=1.026,P=0.00026,Pfdr=0.18)、髓系细胞中的CD33 on CD33dim HLA DR-(OR=1.014,P=0.00050,Pfdr=0.18)以及Treg细胞中的CD45RA+CD28-CD8br%CD8br(OR=1.001,P=0.00078,Pfdr=0.18)与膝骨关节炎风险呈直接的正向因果关联;单核细胞中PDL-1 on monocyte(OR=0.952,P=0.00098,Pfdr=0.18)与膝骨关节炎风险呈直接的负向因果关联。②反向分析结果表明,当膝骨关节炎作为暴露数据时,与731种免疫细胞表型均不具有显著因果关系(FDR<0.20)。③敏感性分析结果显示:双向孟德尔随机化的Cochran’s Q检验和MR-Egger回归法结果P值均大于0.05,表明免疫细胞表型与膝骨关节炎之间的因果效应分析不存在显著的异质性和多效性。④上述结果证实,CD27 on CD24+CD27+,CD33 on CD33dim HLA DR-,CD45RA+CD28-CD8br%CD8br以及PDL-1 on monocyte免疫细胞表型与膝骨关节炎之间可能具有较为显著的潜在因果关系,这为研究膝骨关节炎的生物学机制及探索膝骨关节炎的早期防治提供有价值的线索,也为干预性药物的开发提供了新的方向。