Study progress on anti-lung cancer effects of“Wind-dispelling drugs”

The introduction of the academic ideas such as“the treatment of tumor using Wind-dispelling drugs”and“the treatment of lung cancer should be combined with the governance of Wind”provided new ideas for the treatment of lung cancer.This article summarized the current research progress on anti-lung cancer effects by the two angles of single wind-dispelling drugs and formulas including wind-dispelling drugs.We also put forward the defects of the current research in order to further improve wind-dispelling drugs study and lay the foundation for the treatment of lung cancer.

Regularity of Wind-dispelling Medication Prescribed by LI Dong-Yuan:A Data Mining Technology-based Study

Objective To extract and analyze information on the regularity with which wind-dispelling medication was prescribed by LI Dong-Yuan and many doctors of his school of thought and to provide theoretical basis and ideas for modern clinical application to facilitate the use,research,and development of these medications.Methods Original data on wind-dispelling medication described in LI Dong-Yuan’s works were collected,sorted and organized.Data mining and comprehensive analysis were performed by using a series of data processing softwares,such as SPSS 22.0,Modeler 18.0 and x Miner.By reviewing LI Dong-Yuan’s writings on wind-dispelling medication,the number of prescriptions for wind-dispelling medication were counted.Frequency of use of wind-dispelling medication was determined,and association rules analysis,factor analysis,and core drug network analysis were used to reveal associations of the symptoms of syndromes and treatment with wind-dispelling medication and to reveal the regularity with which these medications were prescribed by LI Dong-Yuan.Results A total of 356 prescriptions and 121 flavors of winddispelling medication were observed.Among them,five kinds of medicinal properties,seven kinds of medicinal tastes,and 12 kinds of meridians were identified.Furthermore,herbs were divided into 18 categories according to their efficacy.Statistical analysis showed that there were 23 wind-dispelling medications that were prescribed with a frequency of≥60.The mainly used drugs were Saposhnikoviae Radix(Fang Feng,防风),Bupleuri Radix(Chai Hu,柴胡),Cimicifugae Rhizoma(Sheng Ma,升麻),Notopterygii Rhizoma Et Radix(Qiang Huo,羌活),Puerariae Lobatae Radix(Ge Gen,葛根),Chuanxiong Rhizoma(Chuan Xiong,川芎),and Ephedrae Herba(Ma Huang,麻黄).In addition,we analyzed the association rules wind-dispelling medication and obtained 23 one-to-one drug association rules and 11 multiple-to-one drug association rules.The core drug network analysis data visualization showed core drugs used in the treatment of external wind assailing the exterior(wind-cold,wind-heat,wind-dampness,etc.),wind-stroke,and tonifying middle and replenishing Qi.Factor analysis was performed on the drugs used by LI Dong-Yuan,and finally 4 groups of wind medicine combinations were obtained.Conclusions This data mining-based study on the regularity of wind-dispelling medication described in LI Dong-Yuan’s works is of great significance,because it reveals the clinical application of its theoretical formulae and medications.

Houshiheisan compound prescription protects neurovascular units after cerebral ischemia

Houshiheisan is composed of wind-dispelling （chrysanthemun fower, divaricate saposhnikovia root, Manchurian wild ginger, cassia twig, Szechwan lovage rhizome, and platycodon root） and deficiency-nourishing （ginseng, Chinese angelica, large-head atractylodes rhizome, Indian bread, and zingiber） drugs. In this study, we assumed these drugs have protective effects against cerebral ischemia, on neurovascular units. Houshiheisan was intragastrically administered in a rat model of focal cerebral ischemia. Hematoxylin-eosin staining, transmission electron microscopy, immu- nofluorescence staining, and western blot assays showed that Houshiheisan reduced pathological injury to the ischemic penumbra, protected neurovascular units, visibly up-regtflated neuronal nuclear antigen expression, and down-regulated amyloid precursor protein and amyloid-[3 42 expression. Wind-dispelling and deficiency-nourishing drugs maintained NeuN expression to varying degrees, but did not affect amyloid precursor protein or amyloid-~ 42 expression in the ischemic penumbra. Our results suggest that the compound prescription Houshiheisan effectively suppresses abnormal amyloid precursor protein accumulation, reduces amyloid substance depo- sition, maintains stabilization of the internal environment of neurovascular units, and minimizes injury to neurovascular units in the ischemic penumbra.

Houshiheisan and its components promote axon regeneration after ischemic brain injury

Houshiheisan,a classic prescription in traditional Chinese medicine,contains Flos Chrysanthemi,Radix Saposhnikoviae,Ramulus Cinnamomi,Rhizoma Chuanxiong,Radix et Rhizoma Asari,Radix Platycodonis,Rhizoma Atractylodis macrocephalae,Poria,Rhizoma Zingiberis,Radix Angelicae sinensis,Radix et Rhizoma Ginseng,Radix Scutellariae and Concha Ostreae.According to traditional Chinese medicine theory,Flos Chrysanthemi,Radix Saposhnikoviae,Ramulus Cinnamomi,Rhizoma Chuanxiong,Radix et Rhizoma Asari and Radix Platycodonis are wind-dispelling drugs;Rhizoma Atractylodis macrocephalae,Poria,Rhizoma Zingiberis,Radix Angelicae sinensis and Radix et Rhizoma Ginseng are deficiency-nourishing drugs.A large number of randomized controlled trials have shown that Houshiheisan is effective in treating stroke,but its mechanism of action is unknown.Axonal remodeling is an important mechanism in neural protection and regeneration.Therefore,this study explored the effect and mechanism of action of Houshiheisan on the repair of axons after cerebral ischemia.Rat models of focal cerebral ischemia were established by ligating the right middle cerebral artery.At 6 hours after model establishment,rats were intragastrically administered 10.5 g/kg Houshiheisan or 7.7 g/kg wind-dispelling drug or 2.59 g/kg deficiency-nourishing drug.These medicines were intragastrically administered as above every 24 hours for 7 consecutive days.Houshiheisan,and its wind-dispelling and deficiency-nourishing components reduced the neurological deficit score and ameliorated axon and neuron lesions after cerebral ischemia.Furthermore,Houshiheisan,and its wind-dispelling and deficiency-nourishing components decreased the expression of proteins that inhibit axonal remodeling：amyloid precursor protein,neurite outgrowth inhibitor protein A（Nogo-A）,Rho family small GTPase A（Rho A） and Rho-associated kinase 2（Rock2）,and increased the expression of growth associated protein-43,microtubule-associated protein-2,netrin-1,Ras-related C3 botulinum toxin substrate 1（Rac1） and cell division cycle 42（Cdc42）.The effect of Houshiheisan was stronger than wind-dispelling drugs or deficiency-nourishing drugs alone.In conclusion,Houshiheisan,and wind-dispelling and deficiency-nourishing drugs promote the repair of axons and nerve regeneration after cerebral ischemia through Nogo-A/Rho A/Rock2 and Netrin-1/Rac1/Cdc42 signaling pathways.These effects are strongest with Houshiheisan.