[Objective] The aim of this study was to determine the variation of phytic acid content (PAC) and its frequency distribution, as well as the relationship between PAC and protein content, kernel characteristics in 16...[Objective] The aim of this study was to determine the variation of phytic acid content (PAC) and its frequency distribution, as well as the relationship between PAC and protein content, kernel characteristics in 161 Chinese winter wheat cultivars from four regions. [Method] One hundred and sixty-one winter wheat varieties from China Wheat Zones I, II, III and IV (Table 1) were grown in a randomized block de- sign, in the 2009-2011 cropping season; and then the indexes for describing the grain morphological characteristics such as the thousand kernel weight (TKW), kernel length (KL), kernel width (KW) and kernel thickness (KT) were measured; the phytic acid content (PAC), protein content and sedimentation value were also determined; finally, the relationship between PAC and protein content, kernel characteristics were analyzed. [Result] The PAC in the cultivars tested ranged from 0.92% to 1.95% with a mean value of 1.41%. Protein content ranged from 12.60% to 19.20%, with a mean of 15.24%. Most (53.4%) of the wheat genotypes had a PAC value in the range of 1.25% to 1.55%. No significant correlation was found between PAC and protein content, sedimentation value, while protein content and SDS sedimentation value was significant correlated, which suggested the possibility of breeding wheat cultivars that have a low PAC but a high protein content and good gluten quality. There was a high correlation between TKW and KW (,.=0.79), KL (r=0.50) and KT (r=0.64). PAC was found having no significant correlation with TKW, KW, KL and KT. [Conclusion] The result suggests the possibility of breeding wheat cultivars that have a low PAC but high kernel weight.展开更多
Soil management practices affect rhizosphere microorganisms and enzyme activities, which in turn influence soil ecosystem processes. The objective of this study was to explore the effects of different nitrogen applica...Soil management practices affect rhizosphere microorganisms and enzyme activities, which in turn influence soil ecosystem processes. The objective of this study was to explore the effects of different nitrogen application rates on wheat(Triticum aestivum L.) rhizosphere soil microorganisms and enzyme activities, and their temporal variations in relation to soil fertility under supplemental irrigation conditions in a fluvo-aquic region. For this, we established a split-plot experiment for two consecutive years(2014–2015 and 2015–2016) in the field with three levels of soil moisture: water deficit to no irrigation(W1), medium irrigation to(70±5)% of soil relative moisture after jointing stage(W2), and adequate irrigation to(80±5)% of soil relative moisture after jointing stage(W3);and three levels of nitrogen: 0 kg ha^–1(N1), 195 kg ha^–1(N2) and 270 kg ha^–1(N3). Results showed that irrigation and nitrogen application significantly increased rhizosphere microorganisms and enzyme activities. Soil microbiological properties showed different trends in response to N level;the highest values of bacteria, protease, catalase and phosphatase appeared in N2, while the highest levels of actinobacteria, fungi and urease were observed in N3. In addition, these items performed best under medium irrigation(W2) relative to W1 and W3;particularly the maximum microorganism(bacteria, actinobacteria and fungi) amounts appeared at W2, 5.37×10^7 and 6.35×10^7 CFUs g^–1 higher than those at W3 in 2014–2015 and 2015–2016, respectively;and these changes were similar in both growing seasons. Microbe-related parameters fluctuated over time but their seasonality did not hamper the irrigation and fertilization-induced effects. Further, the highest grain yields of 13 309.2 and 12 885.7 kg ha^–1 were both obtained at W2 N2 in 2014–2015 and 2015–2016, respectively. The selected properties, soil microorganisms and enzymes, were significantly correlated with wheat yield and proved to be valuable indicators of soil quality. These results clearly demonstrated that the combined treatment(W2 N2) significantly improved soil microbiological properties, soil fertility and wheat yield on the Huanghuai Plain, China.展开更多
Two winter wheat(Triticum aestivum L.)cultivars, large-spike type Yumai66 and small-spike type Yumai49, were used to study the activities of enzymes involved in starch synthesis in the kernel during grain filling. Sta...Two winter wheat(Triticum aestivum L.)cultivars, large-spike type Yumai66 and small-spike type Yumai49, were used to study the activities of enzymes involved in starch synthesis in the kernel during grain filling. Starch accumulated faster in the kernel of Yumai49 than Yumai66 up to 25 d after anthesis, thereafter starch accumulated faster in the kernel of Yumai66. Starch accumulation in Yumai66 peaked at 20 -25 d after anthesis, while in Yumai49 starch accumulation peaked at 15 -20 d after anthesis and 25 -30 d after anthesis. The first peak was much higher than that of the second. Sucrose content and sucrose synthase activity peaked at 20 and 15 d after anthesis in Yumai66 and Yumai49, respectively. The sucrose content and sucrose synthase activity in Yumai66 were higher than that in Yumai49 during grain filling. ADP-glucose pyro-phosphorylase and starch branching enzyme activity in the kernel of Yumai66 peaked at 20 d after anthesis. while soluble starch synthase activity peaked at 10 and 20 d after anthesis. The second peak was much higher than that of the first.展开更多
In order to obtain genetic information for grain yield, seven genetically diverse wheat cultivars were crossed in an incomplete diallel to study the inheritance of grain yield using F_2 progenies for two years. Signif...In order to obtain genetic information for grain yield, seven genetically diverse wheat cultivars were crossed in an incomplete diallel to study the inheritance of grain yield using F_2 progenies for two years. Significant differences were observed among genotype, year and genotype × year interaction for grain yield, and both general combining ability(GCA) and specific combining ability(SCA) were also highly significant for grain yield, suggesting that the trait was controlled by both additive and non-additive effect. The GCA estimates revealed that the best combiners for grain yield were Yangmai 5 and Ningmai 9. Adequacy tests revealed that data of grain yield was fully adequate for genetic interpretation. Over-dominance genetic effects were important for the expression of grain yield. Grain yield exhibited moderately high value of narrow sense heritability(h_N^2=66.98% and h_N^2=72.37%).展开更多
Granule size distribution of wheat starch is an important characteristic that can affect its chemical composition and the functionality of wheat products. Two high-yield winter wheat cultivars were used to evaluate th...Granule size distribution of wheat starch is an important characteristic that can affect its chemical composition and the functionality of wheat products. Two high-yield winter wheat cultivars were used to evaluate the effects of the application of exogenous ABA or GA during the reproductive phase of the initial grain filling on starch granule size distribution and starch components in grains at maturity. The results indicated that a bimodal curve was found in the volume and surface area distribution of grain starch granules, and a unimodal curve was observed for the number distribution under all treatments. The exogenous ABA resulted in a significant increase in the proportions (both by volume and by surface area) of B-type (〈9.9 Ixm in diameter) starch granules, with a reduction in those of A-type (〉9.9 ~tm) starch granules, while, the exogenous GA3 led to converse effects on size distribution of those starch granules. The exogenous ABA also increased starch, amylose and amylopectin contents at maturity but significantly reduced the ratio of amylose to amylopectin. Application of GA3 significantly reduced starch content, amylopectin content but increased the ratio of amylose to amylopectin. The ratio of amylose to amylopectin showed a significant and negative relationship with the volume proportion of granules 〈9.9 μm, but was positively related to the volume proportion of granules 22.8-42.8 μm.展开更多
北部冬麦区是我国重要的小麦主产区之一,对该麦区历年国审小麦品种进行回溯分析有助于小麦品种资源的合理利用。本研究基于产量与熟期、穗数、穗粒数、千粒重、容重、品质指数、抗病指数和抗寒指数等性状的组合,采用品种-产量×性...北部冬麦区是我国重要的小麦主产区之一,对该麦区历年国审小麦品种进行回溯分析有助于小麦品种资源的合理利用。本研究基于产量与熟期、穗数、穗粒数、千粒重、容重、品质指数、抗病指数和抗寒指数等性状的组合,采用品种-产量×性状组合(GYT,genotype by yield×trait)双标图方法对2003-2023年期间北部冬麦区47个国审小麦品种进行了综合分析和分类评价。结果表明,47个国审小麦品种可划分为4个特征显著的品种类型。其中,Ⅰ型品种综合表现优秀,在产量与早熟性、抗病性、抗寒性、千粒重和容重等性状组合上表现突出,在产量与穗数、穗粒数和品质指数组合上表现优良,在生产上推广应用价值最高,主要包括京麦179、京农16和津麦3118等8个品种。Ⅱ型品种综合表现优良,在产量与品质指数、穗数组合上表现突出,在产量与抗病指数、抗寒指数组合上表现稍差,在生产上推广应用价值较高,但应注意生产安全,主要包括京麦202、京农19和轮选158等13个品种。Ⅲ型品种的产量与抗病和抗寒指数组合最好,但在其余性状组合上表现差,综合生产应用价值有限,可作为抗性亲本。Ⅳ型品种综合表现较差,可选择单性状表现优良的品种作为育种亲本应用。根据各品种在GYT双标图ATA轴上的投影位置,筛选出综合表现优良的京麦179、京农16、津麦3118、京麦189、京麦202、京花12号、京农19、轮选158和中麦623等品种。本研究采用GYT双标图分析方法基于“产量-性状”组合水平对北部冬麦区小麦品种进行综合评价和分类研究,为其他作物和地区的类似研究提供了参考。展开更多
Size distribution of glutenin macropolymer (GMP) particles significantly affects the quality of final products in wheat. Six wheat varieties in eastern China were used for investigating the GMP content, particle siz...Size distribution of glutenin macropolymer (GMP) particles significantly affects the quality of final products in wheat. Six wheat varieties in eastern China were used for investigating the GMP content, particle size distribution and their relation. The results showed that the particle diameter of GMP changed from 0.38 μm to 269 μm. A typical bimodal curve of volume distribution of particles with peak values ranged from 4.44 μm to 5.36 μm and from 45.76 μm to 116.30 μm, respectively. Number distribution of particles showed the typical population with peak values in the range of 0.57-1.00 p.m. Proportions of granules 〈12μm, 12-80 μm and 〉80 μm were in the range of 17.1%-47.8%, 32.1%-50.3% and 10.2%-38.1% of total volume, respectively. The content of GMP was negatively correlated with the volume of GMP particles 〈12μm, but positively correlated with the volume of particles 〉80μm. The results suggested that the greater percentage of larger GMP particles is, the more GMP content there is in wheat grain.展开更多
基金Supported by the National Basic Research Program of China(2009CB118602)Young Backbone Teachers Program of Henan Province(2011)~~
文摘[Objective] The aim of this study was to determine the variation of phytic acid content (PAC) and its frequency distribution, as well as the relationship between PAC and protein content, kernel characteristics in 161 Chinese winter wheat cultivars from four regions. [Method] One hundred and sixty-one winter wheat varieties from China Wheat Zones I, II, III and IV (Table 1) were grown in a randomized block de- sign, in the 2009-2011 cropping season; and then the indexes for describing the grain morphological characteristics such as the thousand kernel weight (TKW), kernel length (KL), kernel width (KW) and kernel thickness (KT) were measured; the phytic acid content (PAC), protein content and sedimentation value were also determined; finally, the relationship between PAC and protein content, kernel characteristics were analyzed. [Result] The PAC in the cultivars tested ranged from 0.92% to 1.95% with a mean value of 1.41%. Protein content ranged from 12.60% to 19.20%, with a mean of 15.24%. Most (53.4%) of the wheat genotypes had a PAC value in the range of 1.25% to 1.55%. No significant correlation was found between PAC and protein content, sedimentation value, while protein content and SDS sedimentation value was significant correlated, which suggested the possibility of breeding wheat cultivars that have a low PAC but a high protein content and good gluten quality. There was a high correlation between TKW and KW (,.=0.79), KL (r=0.50) and KT (r=0.64). PAC was found having no significant correlation with TKW, KW, KL and KT. [Conclusion] The result suggests the possibility of breeding wheat cultivars that have a low PAC but high kernel weight.
基金supported by the National Technology R&D Program of China (2013BAD07B07, 2015BAD26B01 and 2018YFD0300701)
文摘Soil management practices affect rhizosphere microorganisms and enzyme activities, which in turn influence soil ecosystem processes. The objective of this study was to explore the effects of different nitrogen application rates on wheat(Triticum aestivum L.) rhizosphere soil microorganisms and enzyme activities, and their temporal variations in relation to soil fertility under supplemental irrigation conditions in a fluvo-aquic region. For this, we established a split-plot experiment for two consecutive years(2014–2015 and 2015–2016) in the field with three levels of soil moisture: water deficit to no irrigation(W1), medium irrigation to(70±5)% of soil relative moisture after jointing stage(W2), and adequate irrigation to(80±5)% of soil relative moisture after jointing stage(W3);and three levels of nitrogen: 0 kg ha^–1(N1), 195 kg ha^–1(N2) and 270 kg ha^–1(N3). Results showed that irrigation and nitrogen application significantly increased rhizosphere microorganisms and enzyme activities. Soil microbiological properties showed different trends in response to N level;the highest values of bacteria, protease, catalase and phosphatase appeared in N2, while the highest levels of actinobacteria, fungi and urease were observed in N3. In addition, these items performed best under medium irrigation(W2) relative to W1 and W3;particularly the maximum microorganism(bacteria, actinobacteria and fungi) amounts appeared at W2, 5.37×10^7 and 6.35×10^7 CFUs g^–1 higher than those at W3 in 2014–2015 and 2015–2016, respectively;and these changes were similar in both growing seasons. Microbe-related parameters fluctuated over time but their seasonality did not hamper the irrigation and fertilization-induced effects. Further, the highest grain yields of 13 309.2 and 12 885.7 kg ha^–1 were both obtained at W2 N2 in 2014–2015 and 2015–2016, respectively. The selected properties, soil microorganisms and enzymes, were significantly correlated with wheat yield and proved to be valuable indicators of soil quality. These results clearly demonstrated that the combined treatment(W2 N2) significantly improved soil microbiological properties, soil fertility and wheat yield on the Huanghuai Plain, China.
文摘Two winter wheat(Triticum aestivum L.)cultivars, large-spike type Yumai66 and small-spike type Yumai49, were used to study the activities of enzymes involved in starch synthesis in the kernel during grain filling. Starch accumulated faster in the kernel of Yumai49 than Yumai66 up to 25 d after anthesis, thereafter starch accumulated faster in the kernel of Yumai66. Starch accumulation in Yumai66 peaked at 20 -25 d after anthesis, while in Yumai49 starch accumulation peaked at 15 -20 d after anthesis and 25 -30 d after anthesis. The first peak was much higher than that of the second. Sucrose content and sucrose synthase activity peaked at 20 and 15 d after anthesis in Yumai66 and Yumai49, respectively. The sucrose content and sucrose synthase activity in Yumai66 were higher than that in Yumai49 during grain filling. ADP-glucose pyro-phosphorylase and starch branching enzyme activity in the kernel of Yumai66 peaked at 20 d after anthesis. while soluble starch synthase activity peaked at 10 and 20 d after anthesis. The second peak was much higher than that of the first.
基金Supported by the Research Projects(CARS-03,BE2013439,CX132021)
文摘In order to obtain genetic information for grain yield, seven genetically diverse wheat cultivars were crossed in an incomplete diallel to study the inheritance of grain yield using F_2 progenies for two years. Significant differences were observed among genotype, year and genotype × year interaction for grain yield, and both general combining ability(GCA) and specific combining ability(SCA) were also highly significant for grain yield, suggesting that the trait was controlled by both additive and non-additive effect. The GCA estimates revealed that the best combiners for grain yield were Yangmai 5 and Ningmai 9. Adequacy tests revealed that data of grain yield was fully adequate for genetic interpretation. Over-dominance genetic effects were important for the expression of grain yield. Grain yield exhibited moderately high value of narrow sense heritability(h_N^2=66.98% and h_N^2=72.37%).
基金supported by the National Natural Science Foundation of China (31271661, 30871477)the National Basic Program of China (2009CB118602)the Special Fund for Agro-Scientific Research in the Public Interest of China (201203100)
文摘Granule size distribution of wheat starch is an important characteristic that can affect its chemical composition and the functionality of wheat products. Two high-yield winter wheat cultivars were used to evaluate the effects of the application of exogenous ABA or GA during the reproductive phase of the initial grain filling on starch granule size distribution and starch components in grains at maturity. The results indicated that a bimodal curve was found in the volume and surface area distribution of grain starch granules, and a unimodal curve was observed for the number distribution under all treatments. The exogenous ABA resulted in a significant increase in the proportions (both by volume and by surface area) of B-type (〈9.9 Ixm in diameter) starch granules, with a reduction in those of A-type (〉9.9 ~tm) starch granules, while, the exogenous GA3 led to converse effects on size distribution of those starch granules. The exogenous ABA also increased starch, amylose and amylopectin contents at maturity but significantly reduced the ratio of amylose to amylopectin. Application of GA3 significantly reduced starch content, amylopectin content but increased the ratio of amylose to amylopectin. The ratio of amylose to amylopectin showed a significant and negative relationship with the volume proportion of granules 〈9.9 μm, but was positively related to the volume proportion of granules 22.8-42.8 μm.
文摘北部冬麦区是我国重要的小麦主产区之一,对该麦区历年国审小麦品种进行回溯分析有助于小麦品种资源的合理利用。本研究基于产量与熟期、穗数、穗粒数、千粒重、容重、品质指数、抗病指数和抗寒指数等性状的组合,采用品种-产量×性状组合(GYT,genotype by yield×trait)双标图方法对2003-2023年期间北部冬麦区47个国审小麦品种进行了综合分析和分类评价。结果表明,47个国审小麦品种可划分为4个特征显著的品种类型。其中,Ⅰ型品种综合表现优秀,在产量与早熟性、抗病性、抗寒性、千粒重和容重等性状组合上表现突出,在产量与穗数、穗粒数和品质指数组合上表现优良,在生产上推广应用价值最高,主要包括京麦179、京农16和津麦3118等8个品种。Ⅱ型品种综合表现优良,在产量与品质指数、穗数组合上表现突出,在产量与抗病指数、抗寒指数组合上表现稍差,在生产上推广应用价值较高,但应注意生产安全,主要包括京麦202、京农19和轮选158等13个品种。Ⅲ型品种的产量与抗病和抗寒指数组合最好,但在其余性状组合上表现差,综合生产应用价值有限,可作为抗性亲本。Ⅳ型品种综合表现较差,可选择单性状表现优良的品种作为育种亲本应用。根据各品种在GYT双标图ATA轴上的投影位置,筛选出综合表现优良的京麦179、京农16、津麦3118、京麦189、京麦202、京花12号、京农19、轮选158和中麦623等品种。本研究采用GYT双标图分析方法基于“产量-性状”组合水平对北部冬麦区小麦品种进行综合评价和分类研究,为其他作物和地区的类似研究提供了参考。
基金Supported by the National Key Research and Development Program of China(2016YFD0300408)the Anhui Provincial Natural Science Foundation(1408085MC48)
文摘Size distribution of glutenin macropolymer (GMP) particles significantly affects the quality of final products in wheat. Six wheat varieties in eastern China were used for investigating the GMP content, particle size distribution and their relation. The results showed that the particle diameter of GMP changed from 0.38 μm to 269 μm. A typical bimodal curve of volume distribution of particles with peak values ranged from 4.44 μm to 5.36 μm and from 45.76 μm to 116.30 μm, respectively. Number distribution of particles showed the typical population with peak values in the range of 0.57-1.00 p.m. Proportions of granules 〈12μm, 12-80 μm and 〉80 μm were in the range of 17.1%-47.8%, 32.1%-50.3% and 10.2%-38.1% of total volume, respectively. The content of GMP was negatively correlated with the volume of GMP particles 〈12μm, but positively correlated with the volume of particles 〉80μm. The results suggested that the greater percentage of larger GMP particles is, the more GMP content there is in wheat grain.