目的探讨Wnt3a在慢性牙周炎患者牙龈组织中的表达及其与附着丧失的关系。方法选择15例慢性牙周炎患者(牙周炎组)和15例牙周健康者(对照组),临床检查记录2组的探诊深度和附着丧失水平,采用RT-PCR和Western-Blot检测牙龈组织中Wnt3a的表...目的探讨Wnt3a在慢性牙周炎患者牙龈组织中的表达及其与附着丧失的关系。方法选择15例慢性牙周炎患者(牙周炎组)和15例牙周健康者(对照组),临床检查记录2组的探诊深度和附着丧失水平,采用RT-PCR和Western-Blot检测牙龈组织中Wnt3a的表达水平,并分析Wnt3a蛋白表达与附着丧失的相关性。结果牙周炎组的探诊深度高于对照组(mm:7.93±1.56 vs 1.83±0.37),其临床附着丧失为(7.76±1.34)mm,而对照组的口腔检查中未见临床附着。与对照组比较,牙周炎组的牙龈组织中Wnt3a m RNA(0.49±0.03 vs 0.36±0.04)和蛋白的表达水平(0.57±0.14 vs 0.43±0.17)均较低(t分别为12.015和2.381,P<0.05)。并且,附着丧失的程度与牙周炎患者牙龈组织中Wnt3a蛋白的表达呈负相关(r=-0.564,P=0.028)。结论 Wnt3a在慢性牙周炎中表达下调,与慢性牙周炎的附着丧失有密切关系,可能在慢性牙周炎的骨吸收中起到了一定的作用。展开更多
Background Bone morphogenetic protein 9 （BMP9） and Wnt/13-catenin signaling pathways are able to induce osteogenic differentiation of mesenchymal stem cells （MSCs）, but the role of Wnt/13-catenin signaling pathway...Background Bone morphogenetic protein 9 （BMP9） and Wnt/13-catenin signaling pathways are able to induce osteogenic differentiation of mesenchymal stem cells （MSCs）, but the role of Wnt/13-catenin signaling pathway in BMP9-induced osteogenic differentiation is not well understood. Thus, our experiment was undertaken to investigate the interaction between BMP9 and Wnt/13-catenin pathway in inducing osteogenic differentiation of MSCs. Methods C3H10T1/2 cells were infected with recombinant adenovirus expressing BMP9, Wnt3a, and BMPg＋Wnt3a. ALP, the early osteogenic marker, was detected by quantitative and staining assay. Later osteogenic marker, mineral calcium deposition, was determined by Alizarin Red S staining. The expression of osteopotin （OPN）, osteocalcin （OC）, and Runx2 was analyzed by Real time PCR and Western blotting. In vivo animal experiment was carried out to further confirm the role of Wnt3a in ectopic bone formation induced by BMP9. Results The results showed that Wnt3a enhanced the ALP activity induced by BMP9 and increased the expressions of OC and OPN, with increase of mineral calcium deposition in vitro and ectopic bone formation in vivo. Furthermore, we also found that Wnt3a increased the level of Runx2, an important nuclear transcription factor of BMP9. Conclusion Canonical Wnt/13-catenin signal pathway may play an important role in BMP9-induced osteogenic differentiation of MSCs, and Runx2 may be a linkage between the two signal pathways.展开更多
文摘目的探讨Wnt3a在慢性牙周炎患者牙龈组织中的表达及其与附着丧失的关系。方法选择15例慢性牙周炎患者(牙周炎组)和15例牙周健康者(对照组),临床检查记录2组的探诊深度和附着丧失水平,采用RT-PCR和Western-Blot检测牙龈组织中Wnt3a的表达水平,并分析Wnt3a蛋白表达与附着丧失的相关性。结果牙周炎组的探诊深度高于对照组(mm:7.93±1.56 vs 1.83±0.37),其临床附着丧失为(7.76±1.34)mm,而对照组的口腔检查中未见临床附着。与对照组比较,牙周炎组的牙龈组织中Wnt3a m RNA(0.49±0.03 vs 0.36±0.04)和蛋白的表达水平(0.57±0.14 vs 0.43±0.17)均较低(t分别为12.015和2.381,P<0.05)。并且,附着丧失的程度与牙周炎患者牙龈组织中Wnt3a蛋白的表达呈负相关(r=-0.564,P=0.028)。结论 Wnt3a在慢性牙周炎中表达下调,与慢性牙周炎的附着丧失有密切关系,可能在慢性牙周炎的骨吸收中起到了一定的作用。
基金This work was supported by a grant from the National Natural Science Foundation of China （No. 31070875）.
文摘Background Bone morphogenetic protein 9 （BMP9） and Wnt/13-catenin signaling pathways are able to induce osteogenic differentiation of mesenchymal stem cells （MSCs）, but the role of Wnt/13-catenin signaling pathway in BMP9-induced osteogenic differentiation is not well understood. Thus, our experiment was undertaken to investigate the interaction between BMP9 and Wnt/13-catenin pathway in inducing osteogenic differentiation of MSCs. Methods C3H10T1/2 cells were infected with recombinant adenovirus expressing BMP9, Wnt3a, and BMPg＋Wnt3a. ALP, the early osteogenic marker, was detected by quantitative and staining assay. Later osteogenic marker, mineral calcium deposition, was determined by Alizarin Red S staining. The expression of osteopotin （OPN）, osteocalcin （OC）, and Runx2 was analyzed by Real time PCR and Western blotting. In vivo animal experiment was carried out to further confirm the role of Wnt3a in ectopic bone formation induced by BMP9. Results The results showed that Wnt3a enhanced the ALP activity induced by BMP9 and increased the expressions of OC and OPN, with increase of mineral calcium deposition in vitro and ectopic bone formation in vivo. Furthermore, we also found that Wnt3a increased the level of Runx2, an important nuclear transcription factor of BMP9. Conclusion Canonical Wnt/13-catenin signal pathway may play an important role in BMP9-induced osteogenic differentiation of MSCs, and Runx2 may be a linkage between the two signal pathways.
