Infection of Wolbachia in Trichogramma cacoeciae

Wolbachia is a symbiotic bacterium that alters reproductive behavior of numerous arthropods. It is the most advanced way to study phytogeny and classification based on sequences of the wsp gene. The presence of Wolbachia in a lab strain of the thelytokous Trichogramma cacoeciae was firstly identified based on amplification and sequencing part of the wsp gene. Aligned the resulting sequence with the published ones, the phylogenetic relationships among Wolbachia which was found in T. cacoeciae, T. dendrolimi and other insects were established. The Wolbachia that found in T. cacoeciae and in T. dendrolimi belong to the Kue group and the Pip group, respectively.

米蛾体内Wolbachia的wsp基因序列测定与系统发育分析

Wolbachia是广泛分布于节肢动物体内的一类共生菌,它们参与多种调控寄主的生殖活动机制。通过对wsp基因的特异性扩增和测序,发现了Wolbachia在米蛾Corcyracephalonica(Stainton)体内的感染。利用所测序列和其他已发表的序列建立系统树,结果表明米蛾体内Wolbachia属于B大组的Pip类群,与其寄生物茧蜂及赤眼蜂中的Wolbachia各株系遗传距离相差较远。据此推测米蛾体内感染的Wolbachia不是由寄生物(茧蜂、赤眼蜂)水平传播所致。

Wolbachia在玉米螟赤眼蜂内的三重感染(英文)

Wolbachia是一类广泛存在于节肢动物体内的共生菌。玉米螟赤眼蜂Trichogramma ostriniae是我国玉米田间的优势赤眼蜂种,据报道,赤眼蜂种内有Wolbachia感染。本文利用Wolbachia的16s rDNA和wsp基因引物通过PCR方法对玉米螟赤眼蜂的野生种群进行了调查,发现以wsp基因为鉴定依据,检测的所有个体都感染了3种Wolbachia[wOstGDAa(GenBank accession no.EU157103),wOstGDAb(GenBank accession no.EU157104)和wOstGDB(GenBankaccession no.EU157105)]。本文首次报道了野生赤眼蜂种群内Wolbachia的三重感染率几乎为100%。根据本研究的结果,可以推测当不同种赤眼蜂寄生同一寄主时,Wolbachia可能会在不同赤眼蜂种间进行横向传播。

Wolbachia在我国广赤眼蜂种群内的感染

Wolbachia是广泛分布于节肢动物生殖组织内的一类细胞内共生菌 ,它属于原细菌的α亚类 ,能够通过调控寄主的生殖活动而促进其在寄主种群中的扩散。通过对wsp基因的克隆及PCR_RFLP分析确定了Wolbachia在我国广赤眼蜂种群内的存在 ,并发现有 2种Wolbachia菌系的感染 ,命名为wEvaA和wEvaB。经过克隆分离得到了这 2种Wolbachia的wsp基因序列 ,在GenBank的登录号为AY3 90 2 79和AY3 90 2 80 ,并由基于wsp基因的聚类树中发现 。