Intellectual Property Rights and Authentic Protection of Chinese Wolfberry in Xinjiang

This paper introduced the biological characteristics and medicinal value of medlar(Lycium chinense Miller).It studied Chinese wolfberry industry and its agricultural intellectual property resources in Xinjiang.In addition,it analyzed main problems such as the lag in the construction of authentic medicinal materials bases and insufficient deep processing of related products.Finally,it proposed eight recommendations including cultivating biological species with more medicinal value and creating an advantageous area of characteristic authentic medicinal materials.

Development Strategies of Chaidamu Wolfberry

With the continuous improvement of people s living standards,wolfberry(Lycium chinense Miller)has penetrated into a large number of families.Chaidamu wolfberry,as the main superior product in Northwest China,has brought more economic benefits to Qinghai Province through the cultivation and sale of wolfberry.In recent years,with the aid of the new technology,the yield of Chaidamu wolfberry has increased rapidly,and the market scale has also expanded rapidly with the publicity.In order to help farmers increase their income and ensure economic benefits,it is of great significance to the healthy development of the whole industry of Chaidamu wolfberry and effectively improve people s living standards.This paper combines the actual situation of the wolfberry market and studies its development status.It is expected to provide some ideas for the development of Chaidamu wolfberry.

Exploring the mechanism of hypolipidemic effect of polyphenols in wolfberry based on network pharmacology and molecular docking

To explore the mechanism of hypolipidemic action of wolfberry polyphenols by using network pharmacology and molecular docking.The active ingredients and targets of wolfberry were searched by TCMSP,and the Cytoscape 3.9.0 software was used to construct a“wolfberry component-target”network.The Gene Cards database was used to screen the hyperlipidemic targets and intersect them with the active targets of wolfberry to construct the PPI network using the STRING platform.The gene ontology(GO)function and Kyoto encyclopedia of genes and genomes(KEGG)pathway enrichment analysis of the core targets were carried out on the Metascape platform,and molecular docking of the active ingredients to the core targets was performed using AutoDockTools software.A total of 33 active ingredients and 173 potential targets of wolfberry were screened,including 99 targets related to hyperlipidemia.The results of the analysis of 99 intersecting targets with the components of wolfberry identified the core active ingredients as quercetin,glycitein and atropine.The binding of the major components of wolfberry,including the polyphenolic compounds quercetin and glycitein,as well as atropine to the key targets AKT1,IL6 and TNF may be important mechanisms for the hypolipidemic therapeutic effect.GO functional enrichment analysis involves biological processes,cellular components,and molecular functions.The KEGG pathway enrichment analysis mainly involves the AGE-RAGE signaling pathway,fluid shear stress,and TNF signaling pathway.Molecular docking validated the good binding activity of the targets to the active ingredients.The binding of atropine and the polyphenolic compounds quercetin and glycitein to the key targets AKT1,IL6 and TNF may be an important mechanism for the hypolipidemic therapeutic effect of wolfberry.

Cloning and Functional Characterisation of Carotenoid Cleavage Dioxygenase 4 from Wolfberry

Carotenoid cleavage dioxygenases(CCDs) are a class of enzymes in plants involved in the biosynthesis of apocarotenoids,such as phytohormones,flavour compounds,and other compounds with yet unknown functions.To date,several CCDs have been functionally characterised in plants,but little is known about the CCD4 members.A carotenoid cleavage dioxygenase 4 gene(LcCCD4) was isolated from the leaves of wolfberry(Lycium chinense) to gain insight into its biological function.Multiple sequence alignment and phylogenetic analyses showed that the deduced amino acid sequence of LcCCD4 shares high homology with that of CCD4 proteins from other plants.Expression analysis using semi-quantitative polymerase chain reaction revealed that LcCCD4 was strongly expressed in leaves and flowers and that the expression level was in accordance with β-carotene concentration.LcCCD4 transcripts in fruits tended to decrease as carotenoids accumulated.Recombinant expression of LcCCD4 cleaved β-carotene to produce P-ionone in in vivo assays.These results show that LcCCD4 is a CCD gene that may be involved in producing aromatic apocarotenoids in leaves and flowers,whereas it may be involved in controlling carotenoid accumulation in fruits.

Wolfberry extracts inhibit Aβ1-42 aggregation and rescue memory loss of AD drosophila

This study is to investigate the potential of wolfberry water extracts for the treatment of Alzheimer’s disease(AD)and the relationship between their anti-AD property and the geographic locations in China.Total extracts of all wolfberry samples showed the potent to decrease self-induced Aβ1-42 aggregation and Cu2+-induced Aβ1-42 aggregation and the antioxidant capacity as well as the bioactivity for binding biometal.A transgenic drosophila model assay revealed that TE of S1 and S6(S1-TE and S6-TE)at 100μg/mL demonstrated the potent rescue effect on memory loss compared to the positive control memantine at 20μg/mL.Further study also revealed the polysaccharide extracts could contribute to their anti-AD property,and the anti-AD activity conspicuously depends on growing environment.Wolfberry samples S1 and S6 showed much more potent anti-AD property.Meanwhile this study demonstrated that the Chinese commonly consumed wolfberry in boiling water might have the beneficial function to prevent AD pathology.

Carbon storage in a wolfberry plantation chronosequence established on a secondary saline land in an arid irrigated area of Gansu Province,China

Carbon（C） storage has received significant attention for its relevance to agricultural security and climate change. Afforestation can increase C storage in terrestrial ecosystems, and has been recognized as an important measure to offset CO_2 emissions. In order to analyze the C benefits of planting wolfberry（Lycium barbarum L.） on the secondary saline lands in arid areas, we conducted a case study on the dynamics of biomass carbon（BC） storage and soil organic carbon（SOC） storage in different-aged wolfberry plantations（4-, 7-and 11-year-old） established on a secondary saline land as well as on the influence of wolfberry plantations on C storage in the plant-soil system in an arid irrigated area（Jingtai County） of Gansu Province, China. The C sequestration and its potential in the wolfberry plantations of Gansu Province were also evaluated. An intact secondary saline land was selected as control. Results show that wolfberry planting could decrease soil salinity, and increase BC, SOC and litter C storage of the secondary saline land significantly, especially in the first 4 years after planting. The aboveground and belowground BC storage values in the intact secondary saline land（control） accounted for only 1.0% and 1.2% of those in the wolfberry plantations, respectively. Compared to the intact secondary saline land, the SOC storage values in the 4-, 7-and 11-year-old wolfberry plantations increased by 36.4%, 37.3% and 43.3%, respectively, and the SOC storage in the wolfberry plantations occupied more than 92% of the ecosystem C storage. The average BC and SOC sequestration rates of the wolfberry plantations for the age group of 0–11 years were 0.73 and 3.30 Mg C/（hm^2·a）, respectively. There were no significant difference in BC and SOC storage between the 7-year-old and 11-year-old wolfberry plantations, which may be due in part to the large amounts of C offtakes in new branches and fruits. In Gansu Province, the C storage in the wolfberry plantations has reached up to 3.574 Tg in 2013, and the C sequestration potential of the existing wolfberry plantations was 0.134 Tg C/a. These results indicate that wolfberry planting is an ideal agricultural model to restore the degraded saline lands and increase the C sequestration capacity of agricultural lands in arid areas.

Experiment on the Fattening Effects of Mixed Silage of Wolfberry Branches on Hybrid

[Objectives] This study was conducted to use the feeding value of wolfberry branches and to provide a basis for the rational use of its active ingredients and nutrients and the development of new feed resources. [Methods] Wolfberry branches and whole-plant corn were used to make mixed silage of wolfberry branches, which replaced different proportions of whole-plant corn silage for feeding hybrid mutton sheep, and the feeding effect of the mixed silage of wolfberry branches on hybrid mutton sheep was analyzed, which provides a technical basis for rational utilization of mulberry resources to ruminants. A single factor feeding comparison experiment was carried out. 32 hybrid mutton sheep of 8 months old with conform gender, age, body weight, physiological state and feeding management level were selected and divided into two groups, 16 in each group. [Results] The gross output value of weight gain of the experimental group was 18.06 yuan, which was higher than the CK(12.97 yuan) by 5.09 yuan. [Conclusions] The addition of the mixed silage of wolfberry branches to the diet had a positive effect on the increase of the weight gain of the hybrid mutton sheep and the reduction of the feed conversion ratio compared with the single whole-plant corn silage.

有机碳测井定量评价在北美Wolfberry页岩油区的应用

确定烃源岩总有机碳含量(TOC)是页岩油气资源评价中的关键技术之一,利用测井资料计算岩石中有机碳含量是烃源岩评价中的重要研究领域。北美二叠盆地Wolfberry页岩油区地化资料有限但测井资料丰富,为了获取油区页岩的有机碳含量,本次研究应用Δlog R技术,从取心井入手,建立了油区基于声波时差和电阻率曲线的有机碳含量计算模型,获得了单井连续的TOC曲线和有效烃源岩累计厚度,并"以点到面"从烃源岩的质和量两方面评价油区页岩纵向和横向的分布特征,为Wolfberry页岩油区的甜点预测及资源评价提供直接证据。

Drought stress,mercuric chloride,andβ-mercaptoethanol effects on hydraulic characteristics of three cultivars of wolfberry(Lycium chinense)

In order to further understand the effects of drought stress on hydraulic characteristics and the relationship between hydraulic conductivity and aquaporins or water channels of root systems of three wolfberry cultivars(Lycium chinense Mill.),hydraulic conductivity of 2-yearold pot-grown seedlings was measured under drought stress,rewatering,and treatment with exogenous mercuric chloride andβ-mercaptoethanol.Under moderate and severe drought stress levels,the most significant decrease of hydraulic conductivity was 37.3%and 24.0%,respectively,in the‘Ningqi 5’cultivar compared with the nonstressed control.After rewatering,the rate of recovery in specific conductivity was most rapid in the‘Mengqi 1’cultivar,at 0.058 and 0.072 kg MPa^-1 m^-2 s^-1 h^-1 under moderate and severe drought stress levels,respectively.The‘Mengqi 1’cultivar had the highest recovery degree of hydraulic conductivity under two concentrations ofβ-ME(500 or 1000 lmol L^-1),reaching 82.4%and 88.5%,respectively,of the initial conductivity.The adaptive capacity of hydraulic conductivity in the‘Ningqi 5’cultivar was weaker than in the‘Ningqi 1’and‘Mengqi 1’cultivars under drought stress.The recovery capacity of hydraulic conductivity in‘Mengqi 1’cultivar was stronger than the‘Ningqi 1’and‘Ningqi 5’cultivars after rewatering.Aquaporins of the‘Ningqi 1’cultivar root systems had the highest binding affinity with mercuric chloride,which was the most likely cause in the decrease in hydraulic conductivity,whereas aquaporins of‘Mengqi 1’root systems had the weakest binding affinity.The inhibitory effect of mercuric chloride was readily eliminated byβ-mercaptoethanol in the‘Mengqi 1’cultivar.The hydraulic characteristics of this cultivar were more sensitive to drought,mercuric chloride andβ-mercaptoethanol than the other cultivars.

Effect of Wolfberry Polysaccharide Additive Premixed Feed on Fattening Effect of Tan Sheep

[Objectives]This study was conducted to explore and rationally use the effective value and nutritional components of wolfberry polysaccharide to provide a basis for the use of new feed resources.[Methods]The premixed feed prepared with the wolfberry polysaccharide additive was applied to fattening Tan sheep in Ningxia,and the effect of the wolfberry polysaccharide additive on the fattening Tan sheep was analyzed,so as to provide technical support for the rational utilization of the wolfberry polysaccharide resources.The study adopted a single-factor comparison method,selecting 84 tan sheep in the fattening period with conform gender,feeding management,weight,age,and physiological status,which were divided into 2 test groups and 1 control group,28 in each group.[Results]Within 90 d,the weight gain of the group with 0.1%wolfberry polysaccharide additive increased by 6.98%,showing a significant difference(P<0.05),and the feed conversion ratio decreased by 9.66%,which was significant as well(P<0.05).The gross profit of this group was 125.76 yuan,which was higher than the control group by 57.52 yuan.The group with 0.2%wolfberry polysaccharide additive increased the weight gain by 8.63%,which was significant(P<0.05),and the feed conversion ratio decreased by 10.80%,which was significant as well(P<0.05).The gross profit of group 2 was 125.76 yuan,which was higher than the control group by 63.41 yuan.There were no significant differences in weight gain,feed conversion ratio and gross profit between the groups with 0.1%and 0.2%of wolfberry polysaccharide additive.The results showed that the use of wolfberry polysaccharide additive premixed feed has a positive effect on increasing the weight gain of fattening Tan sheep,reducing the feed conversion ratio,and improving the feed conversion efficiency.The addition of 0.1%and 0.2%of the wolfberry polysaccharide additive premixed feed had no significant differences.[Conclusions]The wolfberry polysaccharide additive has a positive effect on increasing the weight gain of fattening Tan sheep,reducing the feed conversion ratio,and improving the feed conversion efficiency.

A Wild Species of American Wolfberry——Lycium exsertum A. Gray

Lycium exsertum A. Gray is a wild species of wolfberry, which is mainly distributed in the desert area of Arizona, USA. It was introduced from the United States in 2016. The plant grows vigorously, and its morphological characteristics are different from those of domestic Lycium. The introduction of this material can enrich the domestic Lycium germplasm resources, and it can also be applied in the creation of anti-root rot Lycium germplasm materials and the study on reproductive evolution of Lycium species.

Effects of Different Drying Methods on the Quality of Micronized Chinese Wolfberry Powder

[Objectives] To study the effects of different drying methods on quality of Chinese wolfberry powder. [Methods] The physical properties and nutritional contents of Chinese wolfberry powder prepared by hot air drying,vacuum freeze drying,microwave vacuum drying,drum drying and spray drying were measured and analyzed to explore the effects of different drying methods on quality characteristics of Chinese wolfberry powder. [Results] The total sugar content and reducing sugar contents of product: vacuum freeze drying > spray drying > microwave vacuum drying > drum drying > hot air drying; carotene and total flavonoid contents: vacuum freeze drying > microwave vacuum drying > spray drying > drum drying > hot air drying; after drying process,the dietary fiber content of product obtained by drum drying and spray drying was relatively higher,while the Vc content of Chinese wolfberry powder prepared by vacuum freeze drying was the highest. a~*value( redness to greenness) of color: vacuum freeze drying > microwave vacuum drying > spray drying > drum drying > hot air drying; the Chinese wolfberry powder prepared by vacuum freeze drying had the best solubility,hygroscopicity and rehydration ratio. [Conclusions] Microwave vacuum drying was the best method to prepare Chinese wolfberry powder,in order to keep its quality and consider the cost of production.

Autonomous navigation for a wolfberry picking robot using visual cues and fuzzy control

Lycium barbarum commonly known as wolfberry or Goji is considered an important ingredient in Japanese,Korean,Vietnamese,and Chinese food and medicine.It is cultivated extensively in these countries and is usually harvested manually,which is laborintensive and tedious task.To improve the efficiency of harvesting and reduce manual labor,automatic harvesting technology has been investigated by many researchers in recent years.In this paper,an autonomous navigation algorithm using visual cues and fuzzy control is proposed for Wolfberry orchards.At first,we propose a new weightage(2.4B-0.9G-R)to convert a color image into a grayscale image for better identification of the trunk of Lycium barbarum,the minimum positive circumscribed rectangle is used to describe the contours.Then,using the contour points the least square method is used to fit the navigation line and a region of interest(ROI)is computed that improves the realtime accuracy of the system.Finally,a set of fuzzy controllers,for pneumatic steering system,is designed to achieve real-time autonomous navigation in wolfberry orchard.Static image experiments show that the average accuracy rate of the algorithm is above 90%,and the average time consumption is approximately 162 ms,with good robustness and real-time performance.The experimental results show that when the speed is 1 km/h,the maximum lateral deviation is less than 6.2 cm and the average lateral deviation is 2.9 cm,which meets the requirements of automatic picking of wolfberry picking robot in real-world environments.

Wolfberry tree dual-model detection method and orchard target-oriented fertilization system based on photoelectric sensors

Orchard target-oriented fertilizing with on-board sensor technology can improve fertilizer efficiency and reduce environmental pollution.Photoelectric sensors are widely used for object detection because of their low cost and fast response time.This paper presents a Wolfberry tree dual-model detection method and the design of an orchard target-oriented variable-rate fertilization system based on photoelectric sensors.The dual-model detection method includes the Trunk Detection Model(TDM)and Canopy Detection Model(CDM),which can be applied for Wolfberry orchards at the green cluster and mature stages,respectively.A target-oriented fertilization system was designed using the dual-model method,and tested in the lab and Chinese Wolfberry orchard.The laboratory test results showed that the average center offset distances on the condition of detecting trunks,continuous canopies,and discontinuous canopies were 4.1 cm,9.1 cm and 13.1 cm,respectively.The system could ignore the signals from canes when their diameters were less than 16 mm,and also could determine the gaps within a tree when they were less than 21 cm.The orchard test results showed that the system accomplished target-oriented fertilization 95 times for 92 trees at the mature stage.The results indicated that the dual-model detection method could be used for Wolfberry trees or other trees with similar canopy changes at different growth stages.

Variation of Sweet Chemicals in Different Ripening Stages of Wolfberry Fruits

Objective To evaluate the variation of sweet chemicals（sugars and betaine） in fruits of six species in genus Lycium L. i.e. Lycium truncatum, L. cylindricum, L. dasystemum, L. dasystemum var. rubricaulium, L. chinense, and L. barbarum harvested at three different ripening stages. Methods A simple and effective method based on UPLC-ELSD was developed for the simultaneous determination of two monosaccharides（glucose and fructose）, two disaccharides（sucrose and maltose）, one sugar alcohol（xylitol）, and betaine in wolfberry fruits（goji berries） of genus Lycium L. Results The six species of wolfberry fruits harvested at the three different ripening stages were evaluated in sugars and betaine contents. Fructose and glucose were the predominant sugars in mature wolfberry fruits. Fructose, glucose, and betaine, as well as total sugar contents, increased continually over the ripening process. L. truncatum and L. dasystemum had higher contents of sugars and betaine than the other species. Conclusion UPLC-ELSD is a simple, reliable and effective method for analysis of the sweet chemicals in wolfberries. Wolfberry fruits at the different ripening stages were significant different in sweetness. L. truncatum and L. dasystemum could be the potential sources of wolfberry fruit sources.

Mechanism and experimental study on the fruit detachment of Chinese wolfberry through reciprocating vibration

In order to realize the efficient and high-quality mechanical picking for Chinese wolfberry,firstly,the forced reciprocating vibration picking principle of the Chinese wolfberry branch was studied,and the mechanical model of vibration picking was established based on the simplified cantilever model,and the response analysis and solution of all positions for the branch were carried out.At the same time,the critical mechanical model of fruit detachment under the condition of fruit hanging on branches was established,and the theoretical values of inertia force for each component of the branch were obtained.Secondly,through actual measurement and finite element modeling,the natural frequency and forced vibration response simulation for each component of the branch of Chinese wolfberry terminal branch model were both studied,and the relationship between single-point periodic excitation force and high-quality fruit shedding parameters was obtained.Thirdly,according to the conclusion of the picking model,a test bench with many groups of adjustable parameters was built.Finally,the last branch of fruit-hanging Chinese wolf berry for Ningqi No.1 was taken as the experimental object,a four-level orthogonal experiment was designed with three factors:frequency,amplitude and entrance angle.Meanwhile,the net picking rate,damage rate and false picking rate were taken as the evaluating indicators,referring to the comprehensive scores of the three factors.It was concluded that the primary and secondary relations of factors affecting the picking effect are frequency,amplitude and entrance angle,and the best operation parameters are frequency of 20 Hz,amplitude of 15 mm,and entrance angle of 45°,then,a hand-held vibration picker with setting parameters was trial-produced,and the optimal parameter combination was verified in the Chinese wolfberry planting base of the National Chinese wolfberry Engineering and Technology Research Center.The results showed that the net picking rate of ripe Chinese wolfberry was 96.13%,the damage rate of fruit was 1.13%,and the false picking rate was 3.23%,mechanized picking efficiency was 30.28 kg/h,which is 6.65 times that of manual picking.The experimental results are consistent with the simulation results.The research results can provide an important basis for the creation and operation standards of large-scale Chinese wolfberry vibration harvesting equipment.

Effect of Rare Earth Elements on the Induction Frequency of the Somatic Cell Embryo in The Fruit of Chinese Wolfoerry

A part of lanthanides could raise the induction fraquency(IF) of the somatic cell embryo(SCE) in the fruit of Chinese wolfbeny. The effect of thorium and yttrium used for this purpose is not obvious and even plays a role of inhibition when they are used with a concentration of more than 4 ppm.When the combinations of different rare earth elements (RE) are used, the diversity of the effects amongthem is large. Some of them help to raise the iF of the SCE while the others inhibit the generation of SCE.The mischmetal results in the best effect, giving a relative IF of 295.4%.

野西瓜苗对枸杞蚜虫的杀虫活性研究(英文)

[Objective] The contact toxicity of the extracts from Hibiscus trionum L with different solvents against wolfberry aphis was studied.[Method] The drop method was used for the determination of the insecticidal activities.[Result] 95% ethanol extract displayed the higher contact toxicity on wolfberry aphis and the corrected mortality rate of wolfberry aphids increased with the increasing of the concentration and the prolongation of treating time.The corrected mortality rate in 48 h reached 97.04% under the concentration of 50.00 g/L.The regression equation of virulence was y=1.791 0x＋3.750 2 and LC50 =4.986 7 g/L.The insecticidal activities of five extracts against wolfberry aphids were as follows：chloroformpetroleum etherethyl acetaten-butanol water.The corrected mortality rate of chloroform extract in 48 h was 97.04%.[Conclusion] The study provided a certain reference for further research and its development of H.trionum and its development of new types of botanical pesticide.

Four New Dicaffeoylspermidine Derivatives From Lycium barbarum

Four new dicaffeoylspermidine derivatives, lycibarbarspermidines P–S(1–4), were isolated from the fruit of Lycium barbarum(wolfberry).The structures were determined by extensive spectroscopic(HRESIMS, 1 D NMR, and 2 D NMR) analyses and chemical methods.Dicaffeoylspermidine derivatives are a kind of major bioactive and characteristic constituents in wolfberry, and the discovery of 1–4 added new members of this family.