Configuring single-layer MXene nanosheet onto natural wood fiber via C-Ti-C covalent bonds for high-stability Li-S batteries

Lithium-sulfur batteries(LSBs)are considered promising candidates for next-generation battery technologies owing to their outstanding theoretical energy density and cost-effectiveness.However,the low conductivity and polysulfide shuttling effect of S cathodes severely hamper the practical performance of LSBs.Herein,in situ-generated single layer MXene nanosheet/hierarchical porous carbonized wood fiber(MX/PCWF)composites are prepared via a nonhazardous eutectic activation strategy coupled with pyrolysis-induced gas diffusion.The unique architecture,wherein single layer MXene nanosheets are constructed on carbonized wood fiber walls,ensures rapid polysulfide conversion and continuous electron transfer for redox reactions.The C-Ti-C bonds formed between MXene and PCWF can considerably expedite the conversion of polysulfides,effectively suppressing the shuttle effect.An impressive capacity of 1301.1 m A h g^(-1)at 0.5 C accompanied by remarkable stability is attained with the MX/PCWF host,as evidenced by the capacity maintenance of 722.6 m A h g^(-1)after 500 cycles.Notably,the MX/PCWF/S cathode can still deliver a high capacity of 886.8 m A h g^(-1)at a high S loading of 5.6 mg cm^(-2).The construction of two-dimensional MXenes on natural wood fiber walls offers a competitive edge over S-based cathode materials and demonstrates a novel strategy for developing high-performance batteries.

Clinical Observation and Proposed Classification of Vitiliginous Patches by a Wood’s Lamp

Although vitiligo lesion especially in static state is characterized as sharply demarcated and complete depigmented macule, we encounter patients who have various manners of hypopigmented lesions. We examined the 81 lesions using the newly released Wood’s lamp (Woody®) and investigated whether or not vitiliginous lesions could be uniformly classified under Wood’s lamp illumination and also this classification helped to estimate the tendency of repigmentation after treatment. As result, the vitiliginous lesions were categorized into 4 types on intra- and peri-lesions prior to treatment by using the Wood’s lamp. The inside and border of the lesions were classified as follows: clear white, faint, multi-dot, and perifollicular for the inside, and sharp, blunt, confetti, and trichrome for the border. Suggestive residual pigmentation was detected in 73.6% of patients at the first visit and repigmentation was observed in 67.9% of patients at least 3 months after treatment. Lesions with the “clear white” inside pattern showed significantly lower repigmentation frequency in 38.5% of patients compared to others. The borders with 4 enlarged lesions were composed of 3 of confetti-type and one of sharp-type. This preliminary study demonstrated that detailed observation with a Wood’s lamp could be the basis to classify vitiliginous lesions and might be useful for predicting not only disease progression but also repigmentation prior to treatment.

日本对虾(Penaeus japonicus)病原需钠弧菌(Vibrio natriegens)表型与分子特征及LAMP检测方法的建立

采用常规表观生物学特性及16S rRNA、gyrB及rpoA基因同源性检索与系统发育学分析等方法,对分离自江苏连云港某育苗场的大批死亡日本对虾蚤状幼体的优势生长菌进行了综合鉴定。结果表明,其形态和生理生化特征与弧菌属的需钠弧菌相近;16S rRNA、gyrB及rpoA基因同源性检索也均与需钠弧菌相似性最高,分别为98%、89%和95%,且三种基因的NJ系统发育树也均与需钠弧菌聚为一个分支;分离菌的致病性试验表明其半数致死量LD50为1.8×106CFU/ml。综合分离菌的致病性、形态与生理生化特征及基因同源性与系统发育分析结果,认为引起日本对虾蚤状幼体大批死亡的病原为需钠弧菌。基于gyrB基因序列设计1套LAMP特异性引物,建立了需钠弧菌的快速特异性检测方法,可用于由需钠弧菌引起的水产动物疾病的诊断及分子流行病学的调查研究。

A lamp In The House, a lamp in the heart——Katherine Mansfield and her The Doll's House

"When dear old Mrs. Hay went back to town after staying with the Burnells she sent the children a doll's house." There are three girls in the Burnells , one is Isabel---the eldest sister, the second one is Lottie, and the third one is Kezia. Kazia is the heroine of the story. When the marvellous house stood in the garden, the Burnell children sounded as though they were in despair.

Pathogen diagnosis of children sepsis by LAMP technology

Objective:To explore a rapid diagnostic method in neonatal sepsis and bacterial meningitis. Methods:The primers were designed and synthesized hased on 16S rRNA gene of Staphylococcus aureus.Four specimens of Staphylococcus aureus.16 specimens of coagulasenegative Staphylococci.2 specimens of Enterococci.3 specimens of Streptococcus,1 specimen of Micrococcus.3 specimens of Escherichia coli.4 specimens of Klebsiella pneumoniae,3 specimens of Pseudomonas aeruginosa,2 specimens of Enlerohacter cloacae.and 5 specimens of Acinetobacter were tested by loop-mediated isothermal amplification(LAMP) assay.A total of118 clinical specimens of sepsis and non-sepsis were collected and detected with both LAMP assay and blood culture.Results:Bv designing primers specific for Staphylococcus aureus,specimens containing different kinds of pathogens were earned out by LAMP assay,and our data showed LAMP technology lor the specific detection of Staphylococcus aureus in samples was successlully established.All clinical Specimens of sepsis and non-sepsis were tested by both blood cultures and LAMP,and our data showed that compared wit blood culture method,the LAMP technology, showed significantly high detection rale(P 【0.01).Conclusions:As a quick and easy detection of Staphylococcus aureus,the LAMP technology was successfully established,laid the foundation for the diagnosis and treatment of children Staphylococcus aureus sepsis,and showed great promotion and application value.

Effect of Tree Species and Dosage of Rhizomorph Wood on Asexual Propagation of Wild Gastrodia elata. Bl. f. glauca S. Chow in Ganzi

The effects of the tree species and the dosages of Armillariella mellea rhizomoph wood on asexual propagation of wild Gastrodia elata Bl. f. glauca S. Chow were studied in Ganzi prefecture. The results showed that tree species had a very significant effect on asexual reproduction yield of wild Gastrodia elata Bl. f. glauca S. Chow. The drying rate and yield of Gastrodia elata Bl. f. glauca S. Chow cultured by close grained woods such as Quercus spinosa David ex Fr. was extremely significant higher than that cultured by coarse grained wood such as Betula platyphylla Suk. The dosage had a very significant effect on the yield of wild Gastrodia elata Bl. f. glauca S. Chow whereas it had no influence on the drying rate.

Huabao Electrical Appliances Factory——World's Biggest Pendant Lamp Factory

Started with 100 workers and staff, the Guangdong based Huabao Electrical Appliances Factory has now made 500 products in 50 categories. It boasts 2,000 staff and fixed assets worth RMB80 million. In 1988, the factory was cited as an advanced enterprise in earning foreign exchange among light industries nationwide, by the Ministry of Light Industry; and Huabao electrical appliances were named high quality products in an appraisal sponsored by the Ministry of Light Industry.

NiCo2S4/木材液化物碳气凝胶复合材料的制备及电化学性能

以木材液化物为前驱体原料,经凝胶、碳化、活化法制备的碳气凝胶(CA)为基材,通过两步水热法在其骨架表面原位负载NiCo2S4得到NiCo2S4/木材液化物碳气凝胶(NiCo2S4-CA)复合电极材料。利用扫描电子显微镜(SEM)、氮气吸附-脱附实验、傅里叶红外光谱(FT-IR)、X射线衍射(XRD)、X射线光电子能谱(XPS)等手段来表征NiCo2S4-CA材料的物相结构和表面形貌,通过循环伏安法、恒电流充放电及电化学交流阻抗等测试方法研究其电化学性能,探究其电荷储存机理。结果表明:NiCo2S4纳米颗粒锚定在具有珊瑚网络结构的CA骨架表面,形成丰富的多级孔隙结构。CA的引入有利于NiCo2S4的良好分散,缓解其团聚问题,且不会改变NiCo2S4的晶体结构。NiCo2S4-CA3作为超级电容器电极材料表现出极好的电化学性能,其比容量最大值为1 040.2 F/g(1 A/g),较小的等效电阻为1.01Ω和良好的循环稳定性,经2 000次恒流充放电(10 A/g)循环后,保持着初始比电容的56.61%,优于纯NiCo2S4的47.33%。NiCo2S4-CA3复合材料在20 A/g超大电流密度的电容保持率高达74.34%,远高于纯NiCo2S4的57.65%,电荷储存动力学显示其电化学过程由表面电容控制和扩散控制过程共同作用。该合成工艺简单且成本较低,NiCo2S4-CA3作为超级电容器电极材料在要求高容量、大电流密度领域具有极其广阔的应用前景。

Wood灯检查在白癜风治疗期间不同复色模式观察研究中的应用

目的:通过观察Wood灯下白癜风患者治疗过程中皮损的复色模式,探究不同复色模式的相关影响因素,分析评估不同复色模式间的疗效差异。方法:收集选取2021年8月-2022年8月笔者医院就诊的90例(170块),采集患者基本信息及病情相关指标。治疗后,观察Wood灯下皮损复色模式,将复色模式与收集指标进行统计分析,筛选出差异有统计学意义的指标后,采用Logistic回归分析阳性指标与复色模式的相关性。并将Wood灯下观察的皮损面积作为疗效判定标准,分析对比不同复色模式间的疗效差异。结果:One-way ANOVA分析、χ^(2)检验显示白癜风疾病分期、皮损部位、治疗方式与皮损复色模式有关(P<0.05)。Logistic回归分析显示:治疗方式与复色模式存在相关性(P<0.05)。不同复色模式达到有效复色面积的时间比较,差异有统计学意义(H=8.04,P<0.05)。结论:通过观察Wood灯下白癜风患者治疗过程中皮损的复色模式发现,治疗方式可能是白癜风皮损复色模式的决定因素;不同复色模式之间的复色速度存在差异,可作为临床医师调整治疗方案的参考依据。

木纤维对Sr^(2+)和Cs^+的吸附性能及机理研究

采用间歇法研究了木纤维对Sr2+、Cs+的吸附作用,探讨了木纤维的粒度、溶液的pH、不同介质、吸附时间、温度及木纤维用量等因素对Sr2+、Cs+吸附效果的影响,并用准2级动力学模型、扫描电镜、红外光谱及能谱图分析了吸附机理。结果表明,在室温、木纤维用量为0.2 g、浓度分别均为0.5 mmol·L-1的Sr2+、Cs+溶液50 mL、pH为5.0、振荡吸附时间分别为120和60 min的条件下,Sr2+的吸附去除率达到82.4%,Cs+则只有25%;木纤维对Sr2+、Cs+的吸附使表面形态发生变化,其吸附动力学行为符合准2级吸附速率模型(R2分别为0.999 9和1.000);与Sr2+、Cs+相互作用的基团主要是羟基、碳氢键、碳碳双键和亚甲基,木纤维吸附Sr2+、Cs+的过程中存在离子交换。因此,木纤维对Sr2+、Cs+的属于物理和化学混合吸附类型。

基于有源RFID和LAMP技术的母婴安全监护系统开发

为保障新生儿的健康与安全,结合医院信息化发展的趋势与要求,提出一种基于无线射频(Radio frequency identification,RFID)技术和计算机网络应用的母婴安全监护系统(Maternal-infant Safety and Surveillance System,MISS)的构建方案。在具体的实现中,以有源RFID设备作为底层数据的采集终端,实现对婴儿状态信息的实时获取与存储,以开源LAMP(LINUX+Apache+MySQL+PHP)技术框架作为上层应用的开发基础,实现浏览器/服务器(Browser/Server,B/S)模式下系统软件的总体设计。母婴安全监护系统运维在网络平台之上,可以给监护人员提供便捷的访问服务以及实时可靠的监测信息,为实现母婴智能监护提供了有效的医务决策平台。

基于木塑SLS的图片转浮雕模型3D打印及后处理研究

针对个性化浮雕模型在建筑、工艺行业的应用,首先将二维彩色图片转成灰度图,并将灰度图制作出相应的样式,生成浮雕及其三维模型。随后选择环保、可再生的木塑复合材料作为3D打印基材,通过选择性激光烧结的方法,研究合适的加工工艺,将已生成的三维浮雕进行3D打印。烧结加工参数如下:预热温度为80℃,扫描方向为X、Y方向交替,扫描路径优化为深度-广度混合原则,激光扫描速度2 000 mm/s,扫描线宽0.2 mm,激光功率12 w,分层厚度0.1mm。打印后,使用高压空气清浮粉的方式清粉。最后经过渗蜡、活化处理等后处理工艺以保护基材,降低制件的表面粗糙度、降低材料孔隙率以提高力学强度,对浮雕模型进行化学镀铜,得到质地均匀的金属质感铜镀层。本文研究了个性化浮雕的的加工全过程及其中的关键技术,该方法适用面广、处理速度快、所用材料节能环保,并可根据不同需求进行不同后处理工艺,具有重要的研究价值。

LAMP技术快速诊断布鲁氏菌病的研究

旨在应用环介导等温扩增技术(Loop-mediated isothermal amplification,LAMP)对布鲁氏菌进行研究。针对布鲁氏菌保守基因16S r DNA设计LAMP引物,通过浊度法对LAMP反应条件进行优化,应用建立好的LAMP反应条件进行特异性及灵敏性试验。结果显示:(1)优化后的反应条件是62℃恒温60 min,内引物1.50μmol/μL、外引物0.20μmol/μL、环引物1.0μmol/μL。(2)该LAMP方法与3株布鲁氏菌均发生了扩增反应,达到阳性浊度值,而与小肠耶尔森(ATCC9510)、大肠杆菌(ATCC25922)、沙门氏菌(ATCC10708)和金黄色葡萄球菌(ACTT33591)等标准菌株没有扩增反应。(3)浊度法和实时荧光法两种方法的检测最低限分别为4.36 fg/μL和436 fg/μL,其灵敏度均高于普通PCR的最低检出值4.36 pg/μL。LAMP检测技术用于布病临床诊断具有快速、高效、便捷等特点,对于基层布病的病原学诊断具有实用价值。

RT-LAMP与LAMP法检测结核分枝杆菌的效果比较

目的比较RT-LAMP、LAMP、L-J法检测MTB的效果,为结核病的快速诊断提供依据。方法用常见的非结核分枝杆菌与其他常见的呼吸道感染病菌(金黄色葡萄球菌、铜绿假单胞菌、肺炎克雷伯杆菌)检测特异性,并用酶切实验对结核分枝杆菌特异性产物进行酶切鉴定;用RT-LAMP、LAMP、L-J法检测100名结核病患者和22名来自无结核病患者痰标本中的结核分枝杆菌测试灵敏性;对浓度为1ng/μL H37Rv标准株进行对倍比稀释用于RT-LAMP极限检测实验。结果RT-LAMP、LAMP、L-J方法的阳性率分别为100%,92%和88%;RT-LAMP和L-J,RT-LAMP和LAMP差异均有统计学意义;RT-LAMP的灵敏度比LAMP高10倍;RT-LAMP不仅可以识别活菌,并且能够重复检测MTB的单一拷贝。结论 RTLAMP检测效果优于LAMP及L-J,适于基层医院结核病诊断的推广和应用。

Clinical Application of CLSM,Dermoscope,and Wood’s Lamp in Diagnosis and Differential Diagnosis of Five Hypopigmentation Disorders:A Cross-Sectional Study

Objective:Noninvasive instruments including confocal laser scanning microscope(CLSM),dermoscope,and Wood’s lamp afford the advantage of obtaining skin microanatomical images quickly,in real-time,and dynamically.However,further understanding is needed regarding the differences between various techniques for diagnosis on hypopigmentation diseases.This study was aimed to observe the characteristics of hypopigmentation diseases by using CLSM,dermoscope,and Wood’s lamp,in order to provide evidence for clinician to choose appropriate instruments for assistant diagnosis.Methods:In total,data from 397 patients with vitiligo,nevus anemicus,achromic nevus,pityriasis alba,and senile leukoderma were collected from January 2019 to January 2021 in affiliated hospital of Xuzhou Medical University.CLSM,dermoscope,and Wood’s lamp were used to analyze the characteristics of lesions and the differences between the diseases.Enumeration data were presented as frequencies and percentages.Results:CLSM showed that the pigment ring of vitiligo was absent(126/126,100.0%),senile leukoderma had hypopigmentation,skin atrophy and thinning,and flat epidermis mutation(79/79,100.0%).In dermoscope,vitiligo showed a milky white hypopigmented area(113/126,89.8%),nevus anemicus showed a clear but irregular(38/45,84.4%)white hypopigmentation area(41/45,91.1%),pityriasis alba showed a clear and light white hypopigmented area,with a grayish-white chaff-like scaly appearance(98/107,91.6%),senile leukoderma showed a white radially hypopigmentation area with a small amount of pigmentation(79/79,100.0%).Under Wood’s lamp,bright blue-white patches with clear boundaries can be observed in vitiligo,nevus anemicus,pityriasis alba(93/107,86.9%)and senile leukoderma appeared clear bluish-white patches,achromic nevus appeared unclear bluish-white patches,respectively.Conclusion:Vitiligo,nevus anemicus,achromic nevus,pityriasis alba,and senile leukoderma all belong to hypopigmentation diseases,however,they have own imaging characteristics under CLSM,dermoscope,and Woods lamp techniques,which may be used to help the diagnosis and differential diagnosis of these diseases.

LAMP架构在嵌入式平台上的移植与搭建

基于嵌入式的Web服务应用体系提出将通用服务器架构LAMP应用于嵌入式系统的设计思想。分析LAMP架构在嵌入式领域的应用优势,设计嵌入式平台上LAMP架构的具体实现方案,并给出LAMP中各个组件在移植、配置和优化中的具体方法。该架构为嵌入式系统的高级网络应用提供了一种通用解决方案,其具体实现方法对嵌入式网络服务及其监控系统的应用具有借鉴和参考价值。

改良LAMP法检测转基因作物

目的建立简易的可视化环介导等温扩增(LAMP)检测转基因作物的方法。方法通过一对特异的能识别一段正义链和一段反义链的内引物和一对链置换引物,在Bst DNA聚合酶作用下,以等温条件实现对外源转基因成分的扩增,反应液和高浓度的显色液SYBR GREENⅠ同时置于特殊设计的反应管中,反应后可实现闭管可视化检测。结果对转基因作物中常见的外源基因元件胭脂碱合成酶终止子(T-NOS)和烟草花叶病毒35S启动子(P-CaMV35S)进行了LAMP检测。T-NOS LAMP对大米品系BT63,玉米品系BT11,MON810,大豆品系GTS40-3-2的检测结果呈阳性,P-CaMV35S LAMP对大米品系BT63,玉米品系BT11,MON810,MON863,大豆品系GTS40-3-2的检测结果呈阳性,二者对非转基因大米,玉米,大豆的检测结果均呈阴性。上述结果表明,本文中建立的T-NOS和P-CaMV35S LAMP检测方法特异性高。另外,通过对大豆品系GTS40-3-2模拟样品的检测,证明T-NOS LAMP能检出转基因成分含量为0.5%的样品,P-CaMV35S LAMP能检出转基因成分含量为0.1%的样品,检测灵敏度较高。结论改良LAMP法灵敏度高,特异性好,能用于快速初筛转基因作物。

虾肝肠胞虫LAMP检测技术的建立与应用

本试验根据虾肝肠胞虫(Enterocytozoon hepatopenaei,EHP)18S的保守序列设计4套环介导等温扩增(LAMP)引物,从中筛选出一套引物对LAMP反应体系(Bst DNA聚合酶浓度、内外引物浓度比、dNTP浓度、Mg2+浓度)和反应条件(温度)进行优化。并对优化后的LAMP反应体系和条件进行特异性、灵敏度及精密度验证。特异性试验表明,该方法能特异性检测虾肝肠胞虫,而与其他病害的核酸没有交叉反应;灵敏度试验表明,可检测浓度为10 fg/μL的质粒DNA,制作的标准曲线可对虾肝肠胞虫进行定量分析。重复性试验结果表明,该方法Ct值的变异系数为2.88%~3.02%,具有良好的稳定性。而对于人工侵染的虾肝肠胞虫样品,LAMP方法可检测低至0.44 pg/μL。应用建立的LAMP方法对3个不同地区的120份凡纳滨对虾样品进行检测,检测结果表明,EHP阳性样品数为43,与qPCR检测结果符合率达97%。本试验所建立的LAMP方法具有特异性好、灵敏度高、重复性好的特点,并能在30 min内完成样品的检测,是快速、定量检测虾肝肠胞虫的有效手段。

基于B/S模式的智能用电服务系统设计与开发

针对智能电网下智能终端的应用,结合现有量测方法和计算机网络技术,提出了一种基于B/S模式的智能用电服务系统的实现方案。该系统以智能电表的应用为硬件基础,通过网络协议编程构建用电自动测量网络,并实现对用户用电数据的实时采集。软件则以LAMP(Linux+Apache+MySQL+PHP)为开发平台,综合利用AJAX(异步JavaScript和XML,Asynchronous JavaScript and XML)以及实时数据库技术实现系统的整体设计。该用电智能服务系统以浏览器为用户终端,可以为用户提供综合耗电统计、在线用电付费、智能用电决策以及实时信息交流等便捷而多样化的服务入口,从而为实现用户智能用电提供有力支撑。

毛白杨S-腺苷高半胱氨酸水解酶基因PtSAHHA的克隆、表达及SNP关联分析

组合运用分子生物学及生物信息学方法,首次从毛白杨成熟木质部中分离出编码S-腺苷高半胱氨酸水解酶基因PtSAHHA的cDNA克隆。序列分析表明,毛白杨PtSAHHA cDNA片段全长为1 935 bp,其内部含有大小为1 458 bp的完整开放阅读框架,可编码含有485个氨基酸残基的蛋白质。组织特异性Realtime-PCR结果显示,PtSAHHA在木质部中表达丰度最高,在形成层和老叶中表达丰度中等,在嫩叶中表达丰度最低。在此基础上,组合利用MAGE5.0和DnaSP5软件对毛白杨自然群体中45株基因型个体的PtSAHHA序列进行对比和分析,共检测到166个单核苷酸多态性(SNP)位点,SNP频率为1/15 bp,多样性指数πT为0.010 58。在外显子区域共检测到88个SNP位点,其中同义突变位点34个,错义突变位点53个及1个无义突变位点;同义突变的核苷酸多样性πSynonymous=0.024 91,是非同义突变核苷酸多样性πNonsynonymous=0.002 33的10倍,推测毛白杨PtSAHHA基因编码区在毛白杨物种演化过程中受到较强的纯化选择压力。对PtSAHHA基因内的SNPs连锁不平衡分析结果表明,随着核苷酸序列长度增加至800 bp,即r2<0.1,SNPs的连锁不平衡水平已衰退至不明显。在此基础上,在由426株毛白杨基因型个体组成的关联作图群体内,对频率大于10%的42个常见SNP标记位点与6个木材品质性状进行关联分析,检测到3个SNP标记与3个表型性状显著关联。研究结果为基于分子标记的毛白杨优良木材品质性状定向辅助育种提供理论依据。