Objective: To systematically explore the effects of moderate-intensity static magnetic fields (SMF) on type 2 diabetic wound healing and preliminarily explore the possible mechanism, and hence to lay a foundation for ...Objective: To systematically explore the effects of moderate-intensity static magnetic fields (SMF) on type 2 diabetic wound healing and preliminarily explore the possible mechanism, and hence to lay a foundation for its scientific and extensive clinical application. Methods:Round-shape wound of soft tissues with 1-cm diameter was constructed on the dorsum of thirty-two 3-month-old male type 2 diabetic db/db mice and sixteen wild-type mice with the same gene background, followed by covered with transparent film wound dressing. The experiment was divided into the control group (control), db/db mice group (db/db), and db/db mice exposed to SMF group (db/db+SMF) with sixteen mice in each group. Four mice in each group were killed post 5, 12 and 19 days of the wound model establishment, respectively. The mice in the db/db+SMF group were subjected to systemic SMF exposure (4 mT peak intensity) with 2 h per day. The wound closure rate, overall wound healing period, tensile strength, and histopathological morphology in each group were determined and analyzed. The interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and vascular endothelial growth factor (VEGF) gene expression were also measured and analyzed. Results: SMF significantly increased the wound closure rate (P<0.05), decreased the overall healing period (P<0.05), increased the tensile strength of wound tissues (P<0.05), decreased the number of inflammatory cells, and inhibited the expression of inflammatory cytokines (IL-1β, TNF-αand IL-6) in db/db mice at 5, 12 and 19 days post-surgery (P<0.05), whereas SMF had no significant effect on the VEGF expression. Conclusion: Moderate-intensity systemic SMF exposure exhibits positive therapeutic effects on accelerating type 2 diabetic soft tissue wound repair, and the positive effects are closely related to its significant anti-inflammatory response.展开更多
Wounds debridement is important for healing of chronic wounds. Combined debridement is a new technique to deal with the complex chronic wounds. This review introduces several topical methods of combined debridement ac...Wounds debridement is important for healing of chronic wounds. Combined debridement is a new technique to deal with the complex chronic wounds. This review introduces several topical methods of combined debridement according to the various color classifications. Methods include combined sharp and hydrogel debridement, combined ultrasonic and enzymatic debridement, ultrasonic debridement combined with surgical debridement and vacuum aspiration on debridement, and other types of debridement. This article also explores why each combined debridement mentioned above can achieve a good effect.展开更多
基金This study was supported by the General Project of National Natural Science Foundation of China (Grant 81471806)
文摘Objective: To systematically explore the effects of moderate-intensity static magnetic fields (SMF) on type 2 diabetic wound healing and preliminarily explore the possible mechanism, and hence to lay a foundation for its scientific and extensive clinical application. Methods:Round-shape wound of soft tissues with 1-cm diameter was constructed on the dorsum of thirty-two 3-month-old male type 2 diabetic db/db mice and sixteen wild-type mice with the same gene background, followed by covered with transparent film wound dressing. The experiment was divided into the control group (control), db/db mice group (db/db), and db/db mice exposed to SMF group (db/db+SMF) with sixteen mice in each group. Four mice in each group were killed post 5, 12 and 19 days of the wound model establishment, respectively. The mice in the db/db+SMF group were subjected to systemic SMF exposure (4 mT peak intensity) with 2 h per day. The wound closure rate, overall wound healing period, tensile strength, and histopathological morphology in each group were determined and analyzed. The interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and vascular endothelial growth factor (VEGF) gene expression were also measured and analyzed. Results: SMF significantly increased the wound closure rate (P<0.05), decreased the overall healing period (P<0.05), increased the tensile strength of wound tissues (P<0.05), decreased the number of inflammatory cells, and inhibited the expression of inflammatory cytokines (IL-1β, TNF-αand IL-6) in db/db mice at 5, 12 and 19 days post-surgery (P<0.05), whereas SMF had no significant effect on the VEGF expression. Conclusion: Moderate-intensity systemic SMF exposure exhibits positive therapeutic effects on accelerating type 2 diabetic soft tissue wound repair, and the positive effects are closely related to its significant anti-inflammatory response.
文摘Wounds debridement is important for healing of chronic wounds. Combined debridement is a new technique to deal with the complex chronic wounds. This review introduces several topical methods of combined debridement according to the various color classifications. Methods include combined sharp and hydrogel debridement, combined ultrasonic and enzymatic debridement, ultrasonic debridement combined with surgical debridement and vacuum aspiration on debridement, and other types of debridement. This article also explores why each combined debridement mentioned above can achieve a good effect.
