Injectable and biofunctionalized fibrin hydrogels co-embedded with stem cells induce hair follicle genesis

Fibrin-based hydrogels have been widely used in various tissue engineering because of their biocompatibility,biodegradability,tunable mechanical characteristics and nanofibrous structural properties.However,their ability to support stem cells for hair follicle neogenesis is unclear.In this study,we investigated the effect of fibrin hydrogels in supporting skin-derived precursors(SKPs)in hair follicle neogenesis.Our results showed that SKPs in fibrin hydrogels with high cell viability and proliferation,the stemness of SKPs could be maintained,and the expression of hair induction signature genes such as akp2 and nestin was enhanced.Moreover,hair follicle reconstruction experiments showed de novo hair genesis in mice and the hairs persisted for a long time without teratoma formation.More importantly,the blood vessels and sebaceous glands were also regenerated.Our study demonstrated that fibrin hydrogels are promising in hair follicle regeneration and have potential application in clinical settings for alopecia and wound healing.

不同亚型的真皮成纤维细胞在皮肤再生发育与稳态维持中作用的研究进展

真皮层成纤维细胞分为两种不同的亚型,即真皮浅层的乳头状成纤维细胞(Papillary Fibroblasts,Fp)与真皮深层的网状成纤维细胞(Reticular Fibroblasts,Fr),在细胞功能、分化潜能等方面均存在明显差异,在创伤愈合、毛囊形成与再生以及皮肤老化中的作用亦有不同。本文对两种细胞的形态、功能、来源、分化及特异性表达等方面的不同,以及两者在皮肤再生与稳态维持中的作用进行综述。

创伤性毛囊新生:成年哺乳动物毛囊再生的新视角

目的综述近年来创伤性毛囊新生(wound-induced hair follicle neogenesis,WIHN)细胞来源及相关信号通路的研究进展。方法广泛查阅近年与WIHN相关的文献,从细胞来源、分子机制等方面进行总结讨论。结果WIHN是一种罕见的成年哺乳动物毛囊再生现象,新生毛囊细胞来源丰富,既有创面周围来源的毛囊干细胞,也有上皮来源的干细胞。WIHN分子机制复杂,是一种多种信号通路调控的再生过程,与免疫系统密切相关,免疫细胞及其产生的各种免疫因子为这一过程提供了适宜的条件。结论WIHN的细胞来源及分子机制等方面仍有许多未解决的问题,加强机制研究将加深对成年哺乳动物毛囊再生的了解,为皮肤功能性愈合提供新思路。