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植物乳杆菌WU146-磷酸-β-葡萄糖苷酶的基因克隆、蛋白表达及生物信息学分析
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作者 缪婷婷 沈风飞 +4 位作者 张晓晓 邱胡林 徐波 尹爱国 石鹏君 《中国食品学报》 EI CAS CSCD 北大核心 2022年第6期62-71,共10页
植物乳杆菌是一类兼性异养,兼性厌氧的同型发酵乳酸菌,其在改善食品风味和发酵特性等方面发挥着重要作用,而这种功能特征与其具有糖苷酶活性密切相关。为了挖掘植物乳杆菌糖苷酶的应用价值,以植物乳杆菌WU14为研究对象,利用聚合酶链式反... 植物乳杆菌是一类兼性异养,兼性厌氧的同型发酵乳酸菌,其在改善食品风味和发酵特性等方面发挥着重要作用,而这种功能特征与其具有糖苷酶活性密切相关。为了挖掘植物乳杆菌糖苷酶的应用价值,以植物乳杆菌WU14为研究对象,利用聚合酶链式反应(PCR)技术成功克隆了8个WU14的糖苷水解酶,生物信息学分析表明均为糖苷水解酶1家族(GH1)的6-磷酸-β-葡萄糖苷酶基因。鉴于植物乳杆菌中该类糖苷酶鲜有报道,采用原核异源表达展开研究。序列分析表明8个酶蛋白氨基酸序列具有GH1家族的6-磷酸-β-葡萄糖苷酶典型的2个保守催化位点,序列一致性在32%~74%之间。经SDS-PAGE,8个蛋白均在大肠杆菌中表达,其中BglAW14、BglCW14、BglFW14为部分可溶性表达,其余为蛋白表达,形成包涵体。生物信息学分析显示:8个基因的编码蛋白都无信号肽和跨膜结构,疏水性较强。由亚细胞定位预测可知,除BglEW14主要存在于分泌结构内和BglHW14主要存在于细胞膜外,其余6个基因都存于细胞质内。对8个6-磷酸-β-葡萄糖苷酶基因的克隆表达及生物学序列分析,为进一步探究植物乳杆菌来源的6-磷酸-β-葡萄糖苷酶的分子机理研究奠定了基础。 展开更多
关键词 植物乳杆菌wu14 6-磷酸-β-葡萄糖苷酶 基因克隆 蛋白表达 生物信息学分析
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1-(1,4-苯并二噁烷-2-甲酰)哌嗪的合成 被引量:1
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作者 吴宁苹 赖月琴 徐承策 《化学工业与工程技术》 CAS 2001年第3期32-32,共1页
关键词 1(1 4-苯并二wu-2-甲酰)哌嗪 合成
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A new triterpene saponin from Panax japonicus C.A.Meyer var major(Burk.) C.Y.Wu et K.M.Feng 被引量:4
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作者 Hong Zhao Lin Shi +3 位作者 Jia Qing Cao Wei Li Xia Wen Yu Qing Zhao 《Chinese Chemical Letters》 SCIE CAS CSCD 2010年第10期1216-1218,共3页
One new Iriterpene saponin was isolated from Panaxjaponicus C. A. Meyer var major (Burk.) C. Y. Wu et K. M. Feng, and established as oleanolic acid 3-O-[β-D-glucopyranosyl-(1 →2)-β-D-glucuronopyranosyl-6'-O-n-... One new Iriterpene saponin was isolated from Panaxjaponicus C. A. Meyer var major (Burk.) C. Y. Wu et K. M. Feng, and established as oleanolic acid 3-O-[β-D-glucopyranosyl-(1 →2)-β-D-glucuronopyranosyl-6'-O-n-butyl ester] which showed mod- erate antitumor activities against the A2780 cells and OVCAR-3 cells. Its structure was established by means of spectral data, particularly NMR, including HSQC and HMBC techniques. 展开更多
关键词 Oleanolic acid 3-O--D-glucopyranosyl-(1 2)-β-D-glucuronopyranosyl-6'-O-n-butyl ester] Antitumor Panax japonicus C. A.Meyer vat major (Burk) C Y wu et K. M Feng Triterpene saponin
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On resolution to Wu's conjecture on Cauchy function's exterior singularities
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作者 Theodore Yaotsu Wu 《Acta Mechanica Sinica》 SCIE EI CAS CSCD 2011年第3期309-317,共9页
This is a series of studies on Wu's conjecture and on its resolution to be presented herein. Both are devoted to expound all the comprehensive properties of Cauchy's function f(z) (z = x + iy) and its integral ... This is a series of studies on Wu's conjecture and on its resolution to be presented herein. Both are devoted to expound all the comprehensive properties of Cauchy's function f(z) (z = x + iy) and its integral J[f(z)]≡(2πi)-∮cf(t)(t-z)-1dt taken along the unit circle as contour C,inside which(the open domain D+) f(z) is regular but has singularities distributed in open domain Doutside C. Resolution is given to the inverse problem that the singularities of f(z) can be determined in analytical form in terms of the values f(t) of f(z) numerically prescribed on C(|t| = 1) ,as so enunciated by Wu's conjecture. The case of a single singularity is solved using complex algebra and analysis to acquire the solution structure for a standard reference. Multiple singularities are resolved by reducing them to a single one by elimination in principle,for which purpose a general asymptotic method is developed here for resolution to the conjecture by induction,and essential singularities are treated with employing the generalized Hilbert transforms. These new methods are applicable to relevant problems in mathematics,engineering and technology in analogy with resolving the inverse problem presented here. 展开更多
关键词 Keywords Cauchy function. Singularity distribution . wu's conjecture - Resolution by induction
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Question of Parity Non-conservation in Weak Interactions
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作者 Zuodong Sun 《Journal of Physical Science and Application》 2021年第2期33-49,共17页
In order to reasonably explain the phenomenon of cell bioelectricity,we proposed the conservation law of cell membrane area,established the ion inequality equation,and therefore paid attention to the mystery of“θ-τ... In order to reasonably explain the phenomenon of cell bioelectricity,we proposed the conservation law of cell membrane area,established the ion inequality equation,and therefore paid attention to the mystery of“θ-τ”.We researched and analyzed the“θ-τ”mystery,discussed the parity non-conservation in weak interactions,suggested possible experiments to test the parity non-conservation in weak interactions,and gave our research and analysis conclusions:The parity non-conservation in weak interactions,is still a“conjecture”;The experimental scheme suggested in the papers by C.N.Yang et al.cannot determine whether the weak interaction can separate left and right,and it is impossible to directly answer whetherθandτin the“θ-τ”mystery are the same particle;The Co60βdecay experiment such as C.S.Wu is a pseudo-mirror experiment,whether the experimental result violates parity conservation is only based on the assumption of C.N.Yang et al.In fact,experiments such as polarized Co60 did not overturn the so-called“law of parity conservation”.The mirror image principle does not have any physical meaning,does not correspond to any physical conservation quantity,and cannot be destroyed by any physical experiment.In the process of turning“mirror symmetry”and“mirror asymmetry”into so-called physical“common sense”and scientific“facts”respectively,the methods of transformation are“stealing concepts”and“circular argumentation”.The“θ-τ”mystery is a“man-made”mystery.θandτare two different particles,which may be the result of the same precursor particle being divided into two.The work of C.N.Yang,T.D.Lee,C.S.Wu et al.has brought quantum physicists from the“small black room”to the“bigger black room”or“smaller black room”.The right and wise choice is to go back through the door that came in.With the development of science today,it is time for some contents to reform from the bottom. 展开更多
关键词 Mystery ofθ-τ question of parity non-conservation C.N.Yang T.D.Lee C.S.wu “origami windmill”model.
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WU27Y—25双头弯管机、JH21—200型压力机
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作者 夏萼辉 《锻压机械》 1993年第1期63-63,共1页
最近研制成功WU27Y—25双头弯管机,该机为全液压传动,弯管的夹紧、弯曲、退回全部程序在7~10s内自动完成,生产效率高,适用范围广,特别适用于钢家具、汽车、轮船、飞机等制造业中的座椅、框架状钢管制品的弯管工序。
关键词 wu27Y-25型 双头弯管机 JH21-200型 压力机 锻造
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《历代西域诗钞》编纂得失之我见
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作者 韩留勇 《塔里木大学学报》 2008年第1期72-75,78,共5页
吴蔼宸先生选辑的《历代西域诗钞》作为西域历代诗歌的选编专著,就全书编纂而言,取得了巨大的成就:所选辑诗歌从汉至清,时间跨度长;所选辑诗歌数量众多,内容丰富;所选辑诗歌大都标明出处来历,查有所据。但是也存在明显的不足:全书编纂... 吴蔼宸先生选辑的《历代西域诗钞》作为西域历代诗歌的选编专著,就全书编纂而言,取得了巨大的成就:所选辑诗歌从汉至清,时间跨度长;所选辑诗歌数量众多,内容丰富;所选辑诗歌大都标明出处来历,查有所据。但是也存在明显的不足:全书编纂体例不严密,失误颇多;辑选诗人及其诗作眼界狭隘,忽视本土诗歌创作;辑选比例失调,收罗未尽,多有遗漏。 展开更多
关键词 吴蔼宸 《历代西域诗钞》 编纂 成就 不足
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Were the Miao Kings "Prophets of Renewal"? The Case of the 1795-1797 Hunan Miao Revolt 被引量:1
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作者 Daniel McMahon 《Frontiers of History in China》 2017年第2期301-327,共27页
This essay considers the concept of "prophets of renewal" introduced by James Scott in The Art of Not Being Governed.. An Anarchist History of Upland Southeast Asia (2009), as seen in the context of the 1795-97 Mi... This essay considers the concept of "prophets of renewal" introduced by James Scott in The Art of Not Being Governed.. An Anarchist History of Upland Southeast Asia (2009), as seen in the context of the 1795-97 Miao revolt along China's Hunan-Guizhou border. The appearance ofa "Miao King" and four "Wu kings" centering anti-Qing resistance in an intractable highland--utilizing native legends, spirit possession, investment of officials, and multi-ethnic recruitment--suggests a case of"Zomia" (the vast Southeast Asian Massif) prophets in action, as Scott himself suggests. Closer examination, however, reveals a more complex and uncertain picture, characterized by division between rival lords and an overall dearth of institutional, ideological, or cosmological elaboration, all further obscured by a paucity of historical sources. The Miao kings might be seen as prophets of renewal in a general sense, but the fit is inexact. There is still value, however, in considering Scott's model in the study of this event. It enables a sharper conceptualization of the agency of the Miao people, while offering a case for comparison with analogous instances of religiously-based native resistance on other Qing frontiers. 展开更多
关键词 James Scott prophets of renewal Miao Frontier MIAO 1795-97 Hunan Miao revolt Miao King wu King
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