OBJECTIVE To investigate effects of Wudang cherry on urate excretion and renal function and examined whether renal organic ion transporters were involved in potassium oxonateinduced hyperuricemic mice.METHODS The mode...OBJECTIVE To investigate effects of Wudang cherry on urate excretion and renal function and examined whether renal organic ion transporters were involved in potassium oxonateinduced hyperuricemic mice.METHODS The model of hyperuricemic mice was induced by intraperitoneal injection of potassium oxonate(250 mg·kg^(-1))for 7 d.Water extracts of Wudang cherry at 500 mg·kg^(-1)were orally administered to hyperuricemic mice for 7 d,benzbromarone(20 mg·kg^(-1))and allopurinol(20 mg·kg^(-1))were given as positive controls,vehicle control group was given equal normal saline.Serum and urine levels of uric acid were measured in hyperuricemic and normal mice.Simultaneously,the m RNA and protein levels of mouse urate transporter 1(m URAT1),glucose transporter 9(mGLUT9),organic anion transporters(mOAT1 and mOAT3),ATP-binding cassette,subfamily G,membrane 2(mABCG2)and organic cation/carnitine transporters(m OCT1,m OCT2,m OCTN1 and m OCTN2)in the kidney were analyzed by Western blot,RT-PCR,immunohistochemical and immunofluorescent assay,respectively.RESULTS Wudang cherry significantly reduced serum uric acid levels and increased urine uric acid levels in hyperuricemic mice.And it effectively reversed potassium oxonate-induced alterations in renal m URAT1,mGLUT9,mOAT1,mOAT3 and mABCG2 m RNA and protein levels,resulting in the enhancement of renal urate excretion in mice.Moreover,Wudang Cherry increased renal m OCT1,m OCT2,m OCTN1 and m OCTN2 m RNA and protein levels,and improved renal impairment in this model.CONCLUSION Wudang cherry processes uricosuric and nephroprotective actions by regulating renal organic ion transporters in hyperuricemic mice.展开更多
First, emphasis was given to the analysis on the practices of ecological civilization construction in Wudang District, and then the Wudang mode of ecological civilization construction was discussed and summarized from...First, emphasis was given to the analysis on the practices of ecological civilization construction in Wudang District, and then the Wudang mode of ecological civilization construction was discussed and summarized from the aspects of implementation process, implementation path, and implementation dynamics, obtaining the following enlightenments: accelerating the development of ecological economy is the primary task for the ec- ological civilization construction in western counties; optimizing the spatial development pattern is an important means to promote the ecological civi- lization construction scientifically; capturing the acting point is the key factor to realize the win-win of economic development and ecological protection; the overall development of social undertakings to achieve the happiness of people in rural and urban area is the goal of ecological civilization.展开更多
THE palaces and temples of the ancient building complex in the Wudang Mountains exemplify the architectural and artistic achievements of China's secular and religious buildings of the Yuan (1279-1368). Ming (1368-...THE palaces and temples of the ancient building complex in the Wudang Mountains exemplify the architectural and artistic achievements of China's secular and religious buildings of the Yuan (1279-1368). Ming (1368- 1644) and Qing (1644-1911) dynasties. Towering mountains and deep valleys are partnered with nearby woods, rocks and streams, presenting a harmonious picturesque fusion of man-made architecture and nature.展开更多
The Sr, Nd and Pb isotopic characteristics of the Wudang basic dyke swarms and basic volcanics of the Yaolinghe Group show that they were derived from the same multi\|component mixing source in the mantle. The Wudang ...The Sr, Nd and Pb isotopic characteristics of the Wudang basic dyke swarms and basic volcanics of the Yaolinghe Group show that they were derived from the same multi\|component mixing source in the mantle. The Wudang basic dyke swarms have (\{\}\+\{87\}Sr/\{\}\+\{86\}Sr)\-i=\{0.6905\}~\{0.7061\}, ε\-\{Nd\}(t)=\{-1.9\}~\{5.0\}, Δ\{\}\+\{208\}Pb/\+\{204\}Pb=\{35.49\}~\{190.26\}, Δ\{\}\+\{207\}Pb/\{\}\+\{204\}Pb=4~85, low Th/Ta and a wide range of La/Yb ratios; and the basic volcanics of the Yaolinghe Group have (\{\}\+\{87\}Sr/\{\}\+\{86\}Sr)\-i=\{0.6487\}~\{0.7075\}, ε\-\{Nd\}(t)=\{0.11\}~\{3.94\}, Δ\{\}\+\{208\}Pb/\{\}\+\{204\}Pb=\{-81.58\}~\{219.95\}, Δ\{\}\+\{207\}Pb/\{\}\+\{204\}Pb=\{4.44\}~\{16.68\} and higher Th/Ta and La/Yb ratios, indicating that their source is a mixture of DM and EMII, and the basic volcanics of the Yaolinghe Group were contaminated by crust materials en rout to the surface. Based on the geochemical features of continental tholeiitic basalts and being products of different facies derived from the same source, it can be concluded that an important rifting event in the South Qinling basement block occurred during Neoproterozoic, followed by a setting of oceanic basin in the Early Paleozoic.展开更多
Geochemical characteristics of the Neoproterozoic basic dykes widely exposed in the Wudang block of South Qinling show that the dykes possess the characteristics of continental tholeiitic basalts and were formed in a ...Geochemical characteristics of the Neoproterozoic basic dykes widely exposed in the Wudang block of South Qinling show that the dykes possess the characteristics of continental tholeiitic basalts and were formed in a setting of continental rifting. Therefore, there was an ancient continental block in the areas of southern Qinling and Yangtze block during the Jinning period, and an important rifting event of this ancient block occurred in the Early Neoproterozoic.展开更多
The basic dyke swarms, which have been deformed and metamorphosed, are widespread in the Wudang block. They are geochemically characterized by the continental tholeiites, and their Sm Nd isochron age is (782±164)...The basic dyke swarms, which have been deformed and metamorphosed, are widespread in the Wudang block. They are geochemically characterized by the continental tholeiites, and their Sm Nd isochron age is (782±164) Ma. All of these indicate that there was an ancient continental block in the Proterozoic and a rifting occurring on this continental block during ±800 Ma.展开更多
基金supported by National Natural Science Foundation of China(81402994)Natural Science Foundation of Hubei Province(2014CFB652)"2011 Plan" Open Fund Innovation Team Project of Hubei Province(2011JH-2014CXTT08)
文摘OBJECTIVE To investigate effects of Wudang cherry on urate excretion and renal function and examined whether renal organic ion transporters were involved in potassium oxonateinduced hyperuricemic mice.METHODS The model of hyperuricemic mice was induced by intraperitoneal injection of potassium oxonate(250 mg·kg^(-1))for 7 d.Water extracts of Wudang cherry at 500 mg·kg^(-1)were orally administered to hyperuricemic mice for 7 d,benzbromarone(20 mg·kg^(-1))and allopurinol(20 mg·kg^(-1))were given as positive controls,vehicle control group was given equal normal saline.Serum and urine levels of uric acid were measured in hyperuricemic and normal mice.Simultaneously,the m RNA and protein levels of mouse urate transporter 1(m URAT1),glucose transporter 9(mGLUT9),organic anion transporters(mOAT1 and mOAT3),ATP-binding cassette,subfamily G,membrane 2(mABCG2)and organic cation/carnitine transporters(m OCT1,m OCT2,m OCTN1 and m OCTN2)in the kidney were analyzed by Western blot,RT-PCR,immunohistochemical and immunofluorescent assay,respectively.RESULTS Wudang cherry significantly reduced serum uric acid levels and increased urine uric acid levels in hyperuricemic mice.And it effectively reversed potassium oxonate-induced alterations in renal m URAT1,mGLUT9,mOAT1,mOAT3 and mABCG2 m RNA and protein levels,resulting in the enhancement of renal urate excretion in mice.Moreover,Wudang Cherry increased renal m OCT1,m OCT2,m OCTN1 and m OCTN2 m RNA and protein levels,and improved renal impairment in this model.CONCLUSION Wudang cherry processes uricosuric and nephroprotective actions by regulating renal organic ion transporters in hyperuricemic mice.
基金Supported by the Project of Guiyang Provincial Federation of SocialSciences in 2013
文摘First, emphasis was given to the analysis on the practices of ecological civilization construction in Wudang District, and then the Wudang mode of ecological civilization construction was discussed and summarized from the aspects of implementation process, implementation path, and implementation dynamics, obtaining the following enlightenments: accelerating the development of ecological economy is the primary task for the ec- ological civilization construction in western counties; optimizing the spatial development pattern is an important means to promote the ecological civi- lization construction scientifically; capturing the acting point is the key factor to realize the win-win of economic development and ecological protection; the overall development of social undertakings to achieve the happiness of people in rural and urban area is the goal of ecological civilization.
文摘THE palaces and temples of the ancient building complex in the Wudang Mountains exemplify the architectural and artistic achievements of China's secular and religious buildings of the Yuan (1279-1368). Ming (1368- 1644) and Qing (1644-1911) dynasties. Towering mountains and deep valleys are partnered with nearby woods, rocks and streams, presenting a harmonious picturesque fusion of man-made architecture and nature.
文摘The Sr, Nd and Pb isotopic characteristics of the Wudang basic dyke swarms and basic volcanics of the Yaolinghe Group show that they were derived from the same multi\|component mixing source in the mantle. The Wudang basic dyke swarms have (\{\}\+\{87\}Sr/\{\}\+\{86\}Sr)\-i=\{0.6905\}~\{0.7061\}, ε\-\{Nd\}(t)=\{-1.9\}~\{5.0\}, Δ\{\}\+\{208\}Pb/\+\{204\}Pb=\{35.49\}~\{190.26\}, Δ\{\}\+\{207\}Pb/\{\}\+\{204\}Pb=4~85, low Th/Ta and a wide range of La/Yb ratios; and the basic volcanics of the Yaolinghe Group have (\{\}\+\{87\}Sr/\{\}\+\{86\}Sr)\-i=\{0.6487\}~\{0.7075\}, ε\-\{Nd\}(t)=\{0.11\}~\{3.94\}, Δ\{\}\+\{208\}Pb/\{\}\+\{204\}Pb=\{-81.58\}~\{219.95\}, Δ\{\}\+\{207\}Pb/\{\}\+\{204\}Pb=\{4.44\}~\{16.68\} and higher Th/Ta and La/Yb ratios, indicating that their source is a mixture of DM and EMII, and the basic volcanics of the Yaolinghe Group were contaminated by crust materials en rout to the surface. Based on the geochemical features of continental tholeiitic basalts and being products of different facies derived from the same source, it can be concluded that an important rifting event in the South Qinling basement block occurred during Neoproterozoic, followed by a setting of oceanic basin in the Early Paleozoic.
文摘Geochemical characteristics of the Neoproterozoic basic dykes widely exposed in the Wudang block of South Qinling show that the dykes possess the characteristics of continental tholeiitic basalts and were formed in a setting of continental rifting. Therefore, there was an ancient continental block in the areas of southern Qinling and Yangtze block during the Jinning period, and an important rifting event of this ancient block occurred in the Early Neoproterozoic.
文摘The basic dyke swarms, which have been deformed and metamorphosed, are widespread in the Wudang block. They are geochemically characterized by the continental tholeiites, and their Sm Nd isochron age is (782±164) Ma. All of these indicate that there was an ancient continental block in the Proterozoic and a rifting occurring on this continental block during ±800 Ma.