BACKGROUND Hypertrophic scar(HTS)is dermal fibroproliferative disorder,which may cause physiological and psychological problems.Currently,the potential mechanism of WuFuYin(WFY)in the treatment of HTS remained to be e...BACKGROUND Hypertrophic scar(HTS)is dermal fibroproliferative disorder,which may cause physiological and psychological problems.Currently,the potential mechanism of WuFuYin(WFY)in the treatment of HTS remained to be elucidated.AIM To explore the potential mechanism of WFY in treating HTS.METHODS Active components and corresponding targets were retrieved from the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform.HTSrelated genes were obtained from the GeneCards,DisGeNET,and National Center for Biotechnology Information.The function of targets was analyzed by performing Gene Ontology and Kyoto Encyclopaedia of Genes and Genome(KEGG)enrichment analysis.A protein+IBM-protein interaction(PPI)network was developed using STRING database and Cytoscape.To confirm the high affinity between compounds and targets,molecular docking was performed.RESULTS A total of 65 core genes,which were both related to compounds and HTS,were selected from multiple databases.PPI analysis showed that CKD2,ABCC1,MMP2,MMP9,glycogen synthase kinase 3 beta(GSK3B),PRARG,MMP3,and phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit gamma(PIK3CG)were the hub targets and MOL004941,MOL004935,MOL004866,MOL004993,and MOL004989 were the key compounds of WFY against HTS.The results of KEGG enrichment analysis demonstrated that the function of most genes were enriched in the PI3K-Akt pathway.Moreover,by performing molecular docking,we confirmed that GSK3B and 8-prenylated eriodictyol shared the highest affinity.CONCLUSION The current findings showed that the GSK3B and cyclin dependent kinase 2 were the potential targets and MOL004941,MOL004989,and MOL004993 were the main compounds of WFY in HTS treatment.展开更多
目的研究理疗联合五福饮对椎间盘相关腰痛患者血清β-catenin、疼痛和生活水平的影响。方法选取2017年6月~2018年6月于我院就诊的椎间盘相关腰痛患者126例,按随机数字表法分为实验组(n=63)和对照组(n=63),对照组患者给予常规理疗,实验...目的研究理疗联合五福饮对椎间盘相关腰痛患者血清β-catenin、疼痛和生活水平的影响。方法选取2017年6月~2018年6月于我院就诊的椎间盘相关腰痛患者126例,按随机数字表法分为实验组(n=63)和对照组(n=63),对照组患者给予常规理疗,实验组患者在对照组的基础上给予五福饮治疗,观察两组患者血清β-catenin和DKK1、VAS评分、Oswestry功能障碍指数问卷表(ODI)评分、世界卫生组织生存质量调查表简表(WHOQOL-BREF)评分以及不良反应发生情况。结果治疗后两组患者血清β-catenin和DKK1均显著下降,且实验组血清β-catenin和DKK1显著低于对照组,差异具有统计学意义(P<0.05);治疗后2周、4周和2个月两组患者VAS评分均显著降低,且实验组VAS评分显著低于对照组,差异具有统计学意义(P<0.05);治疗后2周、4周和2个月两组患者ODI评分均显著降低,且实验组ODI评分显著低于对照组,差异具有统计学意义(P<0.05);治疗后两组患者生理领域、心理领域、社会关系领域和环境领域评分均显著升高,且实验组各项评分显著高于对照组,差异具有统计学意义(P<0.05);实验组和对照组不良反应发生率无统计学差异(4.76% vs 1.59%,χ^2=0.529,P=0.427)。结论理疗联合五福饮能显著降低患者血清β-catenin水平,缓解患者疼痛,改善患者生活水平,且具有较高的安全性,值得临床推广。展开更多
基金Supported by the 2022 Shaoxing City Health Science and Technology Program(Health Science and Technology Program),No.2022KY050。
文摘BACKGROUND Hypertrophic scar(HTS)is dermal fibroproliferative disorder,which may cause physiological and psychological problems.Currently,the potential mechanism of WuFuYin(WFY)in the treatment of HTS remained to be elucidated.AIM To explore the potential mechanism of WFY in treating HTS.METHODS Active components and corresponding targets were retrieved from the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform.HTSrelated genes were obtained from the GeneCards,DisGeNET,and National Center for Biotechnology Information.The function of targets was analyzed by performing Gene Ontology and Kyoto Encyclopaedia of Genes and Genome(KEGG)enrichment analysis.A protein+IBM-protein interaction(PPI)network was developed using STRING database and Cytoscape.To confirm the high affinity between compounds and targets,molecular docking was performed.RESULTS A total of 65 core genes,which were both related to compounds and HTS,were selected from multiple databases.PPI analysis showed that CKD2,ABCC1,MMP2,MMP9,glycogen synthase kinase 3 beta(GSK3B),PRARG,MMP3,and phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit gamma(PIK3CG)were the hub targets and MOL004941,MOL004935,MOL004866,MOL004993,and MOL004989 were the key compounds of WFY against HTS.The results of KEGG enrichment analysis demonstrated that the function of most genes were enriched in the PI3K-Akt pathway.Moreover,by performing molecular docking,we confirmed that GSK3B and 8-prenylated eriodictyol shared the highest affinity.CONCLUSION The current findings showed that the GSK3B and cyclin dependent kinase 2 were the potential targets and MOL004941,MOL004989,and MOL004993 were the main compounds of WFY in HTS treatment.
文摘目的研究理疗联合五福饮对椎间盘相关腰痛患者血清β-catenin、疼痛和生活水平的影响。方法选取2017年6月~2018年6月于我院就诊的椎间盘相关腰痛患者126例,按随机数字表法分为实验组(n=63)和对照组(n=63),对照组患者给予常规理疗,实验组患者在对照组的基础上给予五福饮治疗,观察两组患者血清β-catenin和DKK1、VAS评分、Oswestry功能障碍指数问卷表(ODI)评分、世界卫生组织生存质量调查表简表(WHOQOL-BREF)评分以及不良反应发生情况。结果治疗后两组患者血清β-catenin和DKK1均显著下降,且实验组血清β-catenin和DKK1显著低于对照组,差异具有统计学意义(P<0.05);治疗后2周、4周和2个月两组患者VAS评分均显著降低,且实验组VAS评分显著低于对照组,差异具有统计学意义(P<0.05);治疗后2周、4周和2个月两组患者ODI评分均显著降低,且实验组ODI评分显著低于对照组,差异具有统计学意义(P<0.05);治疗后两组患者生理领域、心理领域、社会关系领域和环境领域评分均显著升高,且实验组各项评分显著高于对照组,差异具有统计学意义(P<0.05);实验组和对照组不良反应发生率无统计学差异(4.76% vs 1.59%,χ^2=0.529,P=0.427)。结论理疗联合五福饮能显著降低患者血清β-catenin水平,缓解患者疼痛,改善患者生活水平,且具有较高的安全性,值得临床推广。
