[Objectives]This study was conducted to establish a method for the determination of chlorogenic acid,geniposide,total flavonoids and total triterpenes in Wulan-13.[Methods]The contents of chlorogenic acid and geniposi...[Objectives]This study was conducted to establish a method for the determination of chlorogenic acid,geniposide,total flavonoids and total triterpenes in Wulan-13.[Methods]The contents of chlorogenic acid and geniposide were determined by HPLC,and the contents of total flavonoids and total triterpenes were determined by an ultraviolet spectrophotometer.[Results]There was a good linear relation between the mass of chlorogenic acid reference substance and the peak area in the range of 0.05-0.45μg,and the regression equation was Y=2524.1X+3.1943,(r=0.9998).A good linear relationship was found between the mass of gardenoside reference substance and the peak area in the range of 0.776-6.984μg,and the regression equation was Y=1670.5X+64.804,(r=0.9998).There was also a good linear relation between the mass of rutin reference substance and its absorbance in the range of 0.00808-0.04848 mg,and the regression equation was Y=12.916X+0.014,(r=0.999).The mass of oleanolic acid reference substance had a good linear relation with its absorbance in the range of 0.00418-0.0209 mg,and the regression equation was Y=51.89X-0.0839,(r=0.9991).[Conclusions]The content determination method is simple,reliable and reproducible,and suitable for controlling the contents of chlorogenic acid,geniposide,total flavonoids and total triterpenes in Wulan-13.展开更多
Objective:Xiaoqinglong decoction (XQL) has been used in traditional Chinese medicine as a prescription for asthma treatment.We explored the effects of XQL on mucus hypersecretion and ciliophagy in the airways of mice ...Objective:Xiaoqinglong decoction (XQL) has been used in traditional Chinese medicine as a prescription for asthma treatment.We explored the effects of XQL on mucus hypersecretion and ciliophagy in the airways of mice in which asthma had been induced by ovalbumin (OVA).Methods:Thirty-six mice were sensitized by OVA injection (i.p.) on day-0 and day-14 and challenged with 1% OVA on day 18-22.Then,they were divided into three groups:model,carbocysteine and XQL.From day-15 to day-22,the XQL group was administered XQL (10 g/kg,p.o.) 1 hour before each aerosol challenge with OVA.To evaluate the effect of XQL on mucus hypersecretion,AB-PAS staining,measurement of serum levels of interleukin (IL)-13,bronchoalveolar lavage fluid (BALF) analyses,ciliophagy analyses,as well as coexpression of Light Chain 3 (LC3) and acetylated α-tubulin by immunofluorescence staining were undertaken.Results:Treatment with XQL (10 g/kg) attenuated mucus secretion in the airways,and reduced the positive areas of AB-PAS staining in histopathologic lung tissues (P <.05).IL-13 expression in serum (P <.01),OVA-induced inflammatory changes,and the number of white blood cells (P <.01) in BALF samples were also reduced.However,the effect on mucus secretion was less apparent in the carbocysteine group compared with the XQL group.XQL treatment also improved the cilia length and elicited a substantial reduction in ciliophagy and LC3 expression in the tracheal epithelium.Conclusion:XQL can attenuate cilia shortening,aid the clearance function of ciliated epithelial cells,and reduce mucus production in an OVA-induced asthma model in mice.XQL can inhibit mucus hypersecretion and could be a new type of pharmacotherapy.展开更多
目的:研究麻黄细辛附子汤对变应性鼻炎(allergic rhinitis,AR)大鼠INF-γ与IL-13的影响,揭示麻黄细辛附子汤治疗AR的作用机制。方法:将40只wistar大鼠按体质量采用随机区组法分成5组,即正常对照组、模型对照组、扑尔敏治疗组、麻黄细辛...目的:研究麻黄细辛附子汤对变应性鼻炎(allergic rhinitis,AR)大鼠INF-γ与IL-13的影响,揭示麻黄细辛附子汤治疗AR的作用机制。方法:将40只wistar大鼠按体质量采用随机区组法分成5组,即正常对照组、模型对照组、扑尔敏治疗组、麻黄细辛附子汤低剂量组、麻黄细辛附子汤高剂量组,每组8只,造模分为2个阶段,即基础致敏与鼻内激发。基础致敏:实验组使用5%OVA混悬液致敏,腹腔注射,日1次,共7次;对照组以等量生理盐水替代5%OVA混悬液,方法和步骤同实验组。鼻内激发:实验组从第15天起,以5%卵清蛋白(ovalbumin,OVA)混悬液100μL双侧滴鼻,每侧50μL,日1次,共7次;对照组以等量生理盐水替代5%OVA混悬液,方法和步骤同实验组。麻黄细辛附子汤低剂量组:使用麻黄细辛附子汤2 m L·次^(-1)(每毫升含生药0.2 g),1日2次灌胃,共21 d。麻黄细辛附子汤高剂量组:以麻黄细辛附子汤2 m L·次^(-1)(每毫升含生药0.4 g),一日2次灌胃,共21 d。模型组及正常对照组分别使用生理盐水2 m L·次^(-1),日2次灌胃,共21 d。扑尔敏治疗组:0.01%扑尔敏溶液2 m L·次^(-1),日2次灌胃,共21 d。检测麻黄细辛附子汤治疗后血浆INF-γ与IL-13的含量。结果:模型组INF-γ含量(17.4±2.7)μg·L-1显著降低,IL-13(50.3±2.6)μg·L-1显著升高,治疗后麻黄细辛附子汤低剂量组和麻黄细辛附子汤高剂量组INF-γ含量(22.6±3.0;20.4±2.5)μg·L-1含量升高,IL-13(35.0±3.0;34.2±3.3)μg·L-1含量降低。模型组与正常对照组比较有统计学意义(P<0.01)。麻黄细辛附子汤治疗组、扑尔敏治疗组与模型组比较有统计学意义(P<0.01)。AR大鼠INF-γ含量较低,而IL-13含量较高,与正常对照组比较有统计学意义(P<0.01)。结论:麻黄细辛附子汤可以调节免疫失衡功能,通过使INF-γ含量升高,使IL-13含量降低纠正体内Thl和Th2表达失衡而发挥疗效。展开更多
基金Supported by National Natural Science Foundation of China(82274210).
文摘[Objectives]This study was conducted to establish a method for the determination of chlorogenic acid,geniposide,total flavonoids and total triterpenes in Wulan-13.[Methods]The contents of chlorogenic acid and geniposide were determined by HPLC,and the contents of total flavonoids and total triterpenes were determined by an ultraviolet spectrophotometer.[Results]There was a good linear relation between the mass of chlorogenic acid reference substance and the peak area in the range of 0.05-0.45μg,and the regression equation was Y=2524.1X+3.1943,(r=0.9998).A good linear relationship was found between the mass of gardenoside reference substance and the peak area in the range of 0.776-6.984μg,and the regression equation was Y=1670.5X+64.804,(r=0.9998).There was also a good linear relation between the mass of rutin reference substance and its absorbance in the range of 0.00808-0.04848 mg,and the regression equation was Y=12.916X+0.014,(r=0.999).The mass of oleanolic acid reference substance had a good linear relation with its absorbance in the range of 0.00418-0.0209 mg,and the regression equation was Y=51.89X-0.0839,(r=0.9991).[Conclusions]The content determination method is simple,reliable and reproducible,and suitable for controlling the contents of chlorogenic acid,geniposide,total flavonoids and total triterpenes in Wulan-13.
基金This study was supported by the National Natural Science Foundation of China(81403313).
文摘Objective:Xiaoqinglong decoction (XQL) has been used in traditional Chinese medicine as a prescription for asthma treatment.We explored the effects of XQL on mucus hypersecretion and ciliophagy in the airways of mice in which asthma had been induced by ovalbumin (OVA).Methods:Thirty-six mice were sensitized by OVA injection (i.p.) on day-0 and day-14 and challenged with 1% OVA on day 18-22.Then,they were divided into three groups:model,carbocysteine and XQL.From day-15 to day-22,the XQL group was administered XQL (10 g/kg,p.o.) 1 hour before each aerosol challenge with OVA.To evaluate the effect of XQL on mucus hypersecretion,AB-PAS staining,measurement of serum levels of interleukin (IL)-13,bronchoalveolar lavage fluid (BALF) analyses,ciliophagy analyses,as well as coexpression of Light Chain 3 (LC3) and acetylated α-tubulin by immunofluorescence staining were undertaken.Results:Treatment with XQL (10 g/kg) attenuated mucus secretion in the airways,and reduced the positive areas of AB-PAS staining in histopathologic lung tissues (P <.05).IL-13 expression in serum (P <.01),OVA-induced inflammatory changes,and the number of white blood cells (P <.01) in BALF samples were also reduced.However,the effect on mucus secretion was less apparent in the carbocysteine group compared with the XQL group.XQL treatment also improved the cilia length and elicited a substantial reduction in ciliophagy and LC3 expression in the tracheal epithelium.Conclusion:XQL can attenuate cilia shortening,aid the clearance function of ciliated epithelial cells,and reduce mucus production in an OVA-induced asthma model in mice.XQL can inhibit mucus hypersecretion and could be a new type of pharmacotherapy.
文摘目的:研究麻黄细辛附子汤对变应性鼻炎(allergic rhinitis,AR)大鼠INF-γ与IL-13的影响,揭示麻黄细辛附子汤治疗AR的作用机制。方法:将40只wistar大鼠按体质量采用随机区组法分成5组,即正常对照组、模型对照组、扑尔敏治疗组、麻黄细辛附子汤低剂量组、麻黄细辛附子汤高剂量组,每组8只,造模分为2个阶段,即基础致敏与鼻内激发。基础致敏:实验组使用5%OVA混悬液致敏,腹腔注射,日1次,共7次;对照组以等量生理盐水替代5%OVA混悬液,方法和步骤同实验组。鼻内激发:实验组从第15天起,以5%卵清蛋白(ovalbumin,OVA)混悬液100μL双侧滴鼻,每侧50μL,日1次,共7次;对照组以等量生理盐水替代5%OVA混悬液,方法和步骤同实验组。麻黄细辛附子汤低剂量组:使用麻黄细辛附子汤2 m L·次^(-1)(每毫升含生药0.2 g),1日2次灌胃,共21 d。麻黄细辛附子汤高剂量组:以麻黄细辛附子汤2 m L·次^(-1)(每毫升含生药0.4 g),一日2次灌胃,共21 d。模型组及正常对照组分别使用生理盐水2 m L·次^(-1),日2次灌胃,共21 d。扑尔敏治疗组:0.01%扑尔敏溶液2 m L·次^(-1),日2次灌胃,共21 d。检测麻黄细辛附子汤治疗后血浆INF-γ与IL-13的含量。结果:模型组INF-γ含量(17.4±2.7)μg·L-1显著降低,IL-13(50.3±2.6)μg·L-1显著升高,治疗后麻黄细辛附子汤低剂量组和麻黄细辛附子汤高剂量组INF-γ含量(22.6±3.0;20.4±2.5)μg·L-1含量升高,IL-13(35.0±3.0;34.2±3.3)μg·L-1含量降低。模型组与正常对照组比较有统计学意义(P<0.01)。麻黄细辛附子汤治疗组、扑尔敏治疗组与模型组比较有统计学意义(P<0.01)。AR大鼠INF-γ含量较低,而IL-13含量较高,与正常对照组比较有统计学意义(P<0.01)。结论:麻黄细辛附子汤可以调节免疫失衡功能,通过使INF-γ含量升高,使IL-13含量降低纠正体内Thl和Th2表达失衡而发挥疗效。
