Determination of Chlorogenic acid in Wuli Huichun Wan by HPLC

[Objectives] To establish a method for determining the content of chlorogenic acid in Wuli Huichun Wan. [Methods] The content of chlorogenic acid in Wuli Huichun Wan was determined by high performance liquid chromatography( HPLC) combined with Agilent Eclipse Plus C18 column( 4. 6 mm × 250 mm,5 μm),in mobile phase of 0. 4% phosphoric acid solution-acetonitrile( 12∶ 88) at flow rate of1. 0 m L/min,and detection wavelength of 327 nm. [Results] The linearity of chlorogenic acid in the range of 4-48 μg injection was good( r = 1); the average recovery rate was 99. 61% and the RSD was 0. 98%. [Conclusions] The established method is simple and accurate and has high reproducibility,thus can be used as quality control method for Wuli Huichun Wan.

五粒回春丸联合阿昔洛韦治疗小儿手足口病的应用研究

目的:采用五粒回春丸联合阿昔洛韦治疗小儿手足口病的疗效观察,并观察不良反应发生率。方法:选择2015年1月-2016年6月治疗的小儿手足口病患儿,随机分为两组,对照组45例采用阿昔洛韦治疗,10mg·kg-1·d-1,注入0.9%生理盐水或5%葡萄糖100~200m L中进行静脉滴注,1次/d;观察组45例采用五粒回春丸联合阿昔洛韦治疗。两组患者共治疗5d,观察治疗后的体温恢复时间、口腔溃疡愈合时间、皮疹消退时间、住院时间和中医证候改善情况、统计两组患儿的不良反应发生率。结果:观察组体温恢复时间、口腔溃疡愈合时间、皮疹消退时间、住院时间均少于对照组(P<0.05),对比具有统计学意义;对照组中医证候显著改善20例、部分改善15例、无改善10例,观察组显著改善34例、部分改善7例、无改善4例,经检验表明观察组中医证候改善情况优于对照组(P<0.05),对比具有统计学意义;对照组不良反应发生率为8.89%(4/45),观察组不良反应发生率为6.67%(3/45),两组患儿不良反应发生率对比无差异(P>0.05)。结论:经五粒回春丸联合阿昔洛韦治疗小儿手足口病能有效的缩短患儿的病程,中医证候改善较为明显,无明显的不良反应,临床运用的疗效及安全性较高,值得在临床中推广实施。