[Objective] The aim of this study is to investigate hazard assessment on heavy metal pollution in surface soil from tea gardens of Wuzhishan. [Method] The average content and accumulation status of heavy metals includ...[Objective] The aim of this study is to investigate hazard assessment on heavy metal pollution in surface soil from tea gardens of Wuzhishan. [Method] The average content and accumulation status of heavy metals including Cr,Ni,Cu,Zn,As,Cd and Pb in surface soil from tea gardens of Wuzhishan was collected. Taking the highest global background value of heavy metals in sediments before modern industrialization and the first-class natural background value of Soil Environment Quality Standard in China as the reference value respectively,the accumulation or ecological hazard coefficient and the ecological hazard index of heavy metals in surface soil from tea gardens of Wuzhishan were assessed by potential ecological risk index. [Result] The pollution level with two kinds of background values as the reference value was similar,while Cd and Pb were main heavy metals which had potential impact on ecological environmental in surface soil from tea gardens of Wuzhishan. [Conclusion] The potential risk level of heavy metals in surface soil from tea gardens of Wuzhishan belongs to the slight-medium pollution level,and the heavy metal with potential risk is mainly Cd.展开更多
为了解五指山野生大叶茶(Camellia sinensis var. assamica)代谢物的特征,利用代谢组学方法,对采集自五指山地区的野生茶树和人工茶园茶叶的代谢物成分开展了比较研究,在野生茶树茶叶中鉴定得到398个含量显著上调的代谢物和247个显著下...为了解五指山野生大叶茶(Camellia sinensis var. assamica)代谢物的特征,利用代谢组学方法,对采集自五指山地区的野生茶树和人工茶园茶叶的代谢物成分开展了比较研究,在野生茶树茶叶中鉴定得到398个含量显著上调的代谢物和247个显著下调的代谢物。基于偏最小二乘法判别分析表明,野生茶叶和人工茶叶分别聚为一类。富集分析结果显示,野生茶树叶片中含量显著上升的代谢物的功能主要富集于糖酵解、丙酮酸合成、氨基糖代谢和核苷糖代谢等糖代谢通路,以及酚类物质合成、核黄素代谢、鞘磷脂代谢和黄酮合成等通路,而显著下降的代谢物的功能主要富集于氨基酸代谢和油脂代谢等初生代谢。展开更多
[Objective] cDNA of growth hormone releasing hormone （GHRH） receptor gene from Wuzhishan miniature pig was cloned and its sequence was also analyzed. [Method] Using genomic DNA extracted from porcine ear tissues of ...[Objective] cDNA of growth hormone releasing hormone （GHRH） receptor gene from Wuzhishan miniature pig was cloned and its sequence was also analyzed. [Method] Using genomic DNA extracted from porcine ear tissues of Wuzhishan miniature pig as the template, three pairs of primers were designed by the reported cDNA sequence of porcine GHRH, and cDNA was also amplified by RT-PCR. After being recovered and purified, PCR products were ligated to pMD18-T and then transformed into Escherichia coli DH5a. The transformation products were analyzed by PCR and double enzyme digestion to screen positive clones, and the positive clones were sequenced after identification in LB liquid medium. [ Result] cDNA of Wuzhishan miniature pig GHRH receptor gene was obtained successfully, and its length was 1 577 bp coding 423 amino acids. BLAST analysis showed that there were only 23 nuoleotides in difference between this fragment and pomine GHRH receptor gene, and its homology was 98%. However, both GHRH receptor genes were constituted by 423 amino acids with the sequence homology of 96%. [ Conclusion] This study provides theoretical basis for further studies on the dwarf mechanism of Wuzhishan miniature pig.展开更多
[ Objective] The study aimed to lay a foundation for studying immune recognition mechanism in xenotransplantation. [ Method] SLA- DQA gene of Hainan Wuzhishan miniature pig was cloned by RT-PCR and sequenced. J-Result...[ Objective] The study aimed to lay a foundation for studying immune recognition mechanism in xenotransplantation. [ Method] SLA- DQA gene of Hainan Wuzhishan miniature pig was cloned by RT-PCR and sequenced. J-Result] The SLA-DQA gene sequence of Wuzhishan minia- ture pig contained 768 nucleotides, among which 1 -765 were the complete open reading frame, encoding 255 amino acids. SLA-DQA cDNA se- quence and its deduced amino acid sequence of Wuzhishan miniature pig were most similar to those of the other miniature pigs in China. When compared with corresponding cDNA sequences and the amino acid sequences in other species, the homology with human was obviously higher than that with mouse. [ Conclusion] As the Chinese pig breed suitable for xenotransplantation, Wuzhishan miniature pig has significant importance to ser- ial studies on immunogenetics characteristics.展开更多
To elucidate the structure of SLA-3 alleles on inbred line of Wuzhishan pig （WZSP） population, we examined the partial exon 1, completed exon 2, and partial exon 3 of SLA-3 loci using the reverse transcription-polym...To elucidate the structure of SLA-3 alleles on inbred line of Wuzhishan pig （WZSP） population, we examined the partial exon 1, completed exon 2, and partial exon 3 of SLA-3 loci using the reverse transcription-polymerase chain reaction （RT- PCR） and the sequencing-based method in 32 WZSPs. According to pedigree and amplification results, PCR products of 8 WZSPs were selected to clone and sequence. Nine different nucleotide sequences were obtained. After comparing the DNA and protein sequences of the WZSPs SLA-3 alleles with the published GenBank SLA sequences, it was found that the SLA-3 alleles in WZSPs were all novel, but there were very few variations among them. Comparision of SLA-3 and HLA-A protein sequences indicated that there was more sequence homology. Meanwhile, the construction of a phylogenetic tree using the nucleotide sequences of 23 SLA-3 alleles and 1 HLA-A allele represented that the WZSP population owns its unique genetics resource. In this study, the alleles of SLA-3 on WZSP group were successfully detected and analyzed, which provided the firm basis on the genotype of SLA-3 for breeding specific haplotypes WZSPs.展开更多
In order to obtain the TLR 4 gene sequence of Hainan Wuzhishan pig and analyze its molecular structure characteristics,its genomic sequence was cloned and sequenced by PCR and subjected to related bioinformatics analy...In order to obtain the TLR 4 gene sequence of Hainan Wuzhishan pig and analyze its molecular structure characteristics,its genomic sequence was cloned and sequenced by PCR and subjected to related bioinformatics analysis with the genomic sequence of the TLR 4 gene of pig in GenBank as a reference sequence.The full-length DNA sequence of Wuzhishan pig TLR 4 gene was 10 435 bp in length,of which CDS was 2 526 bp in length and encoded 841 amino acids.Compared with the common pig TLR 4 gene sequence published by GenBank,there were 38 mutations,of which two mutations were in the CDS region.The results of homology analysis showed that the amino acid sequence of the TLR 4 gene of Wuzhishan pig shared 99.9%,80.7%,79.9%,74.8%,74.7%,73.1%,73.0%,72.7%,62.5% and 43.4% homology with those of common pig,cattle,sheep,dog,horse,macaque,human,chimpanzee,mouse and chicken,respectively.The protein encoded by the TLR 4 of Wuzhishan pig belonged to secreted protein and transmembrane protein.It was hydrophilic,and had 8 glycosylation modification sites,17 serines (ser),7 threonines (Thr) and 8 tyrosine (TYR) phosphorylation sites.The amino acid phylogenetic tree analysis showed that the TLR 4 genes of different species are highly conserved during evolution.This result lays a foundation for further study of the function of TLR 4 gene.展开更多
As the protected areas of land and coastal environment, nature reserves are designed to address how to reconcile the conservation of biodiversity, the quest for economic and social development and the maintenance of c...As the protected areas of land and coastal environment, nature reserves are designed to address how to reconcile the conservation of biodiversity, the quest for economic and social development and the maintenance of cultural values. This paper establishes a framework for nature reserve development that seeks to incorporate ecotourism into its strategies. The overall purpose was to identify the information needs required for a comprehensive nature reserve that incorporates ecotourism related values. It also illustrates the utility of this framework in the context of the Wuzhishan Mountain Region of China. A literature review, the first phase of a visionary strategy and a subsequent gap analysis for available management information were undertaken in order to achieve this paper＇s purpose. Finally, recommendations axe pre-sented for integrating ecotourism into nature reserve development in the Wuzhishan Mountain Region.展开更多
1 Introduction The Wuzhishan lead-zinc ore-concentrated area in Puding is located in the east of the Sichuan,Guizhou and Yunnan lead-zinc metallogenic domain,with the Youjiang-Nanpan River metallogenic province to the
基金Supported by Science and Technology Foundation in Hainan(40879 )Project by Hainan Education Department ( HJKJ2010-28)+1 种基金Scientific Research Initial Fund for Returned Students by Ministry of EducationJoint Project of Key Subjects with "Cartology &Geographic Information System" and "Natural Geography" by Hainan Normal University~~
文摘[Objective] The aim of this study is to investigate hazard assessment on heavy metal pollution in surface soil from tea gardens of Wuzhishan. [Method] The average content and accumulation status of heavy metals including Cr,Ni,Cu,Zn,As,Cd and Pb in surface soil from tea gardens of Wuzhishan was collected. Taking the highest global background value of heavy metals in sediments before modern industrialization and the first-class natural background value of Soil Environment Quality Standard in China as the reference value respectively,the accumulation or ecological hazard coefficient and the ecological hazard index of heavy metals in surface soil from tea gardens of Wuzhishan were assessed by potential ecological risk index. [Result] The pollution level with two kinds of background values as the reference value was similar,while Cd and Pb were main heavy metals which had potential impact on ecological environmental in surface soil from tea gardens of Wuzhishan. [Conclusion] The potential risk level of heavy metals in surface soil from tea gardens of Wuzhishan belongs to the slight-medium pollution level,and the heavy metal with potential risk is mainly Cd.
文摘为了解五指山野生大叶茶(Camellia sinensis var. assamica)代谢物的特征,利用代谢组学方法,对采集自五指山地区的野生茶树和人工茶园茶叶的代谢物成分开展了比较研究,在野生茶树茶叶中鉴定得到398个含量显著上调的代谢物和247个显著下调的代谢物。基于偏最小二乘法判别分析表明,野生茶叶和人工茶叶分别聚为一类。富集分析结果显示,野生茶树叶片中含量显著上升的代谢物的功能主要富集于糖酵解、丙酮酸合成、氨基糖代谢和核苷糖代谢等糖代谢通路,以及酚类物质合成、核黄素代谢、鞘磷脂代谢和黄酮合成等通路,而显著下降的代谢物的功能主要富集于氨基酸代谢和油脂代谢等初生代谢。
基金Supported by Natural Science Foundation of Hainan Province(30515)~~
文摘[Objective] cDNA of growth hormone releasing hormone （GHRH） receptor gene from Wuzhishan miniature pig was cloned and its sequence was also analyzed. [Method] Using genomic DNA extracted from porcine ear tissues of Wuzhishan miniature pig as the template, three pairs of primers were designed by the reported cDNA sequence of porcine GHRH, and cDNA was also amplified by RT-PCR. After being recovered and purified, PCR products were ligated to pMD18-T and then transformed into Escherichia coli DH5a. The transformation products were analyzed by PCR and double enzyme digestion to screen positive clones, and the positive clones were sequenced after identification in LB liquid medium. [ Result] cDNA of Wuzhishan miniature pig GHRH receptor gene was obtained successfully, and its length was 1 577 bp coding 423 amino acids. BLAST analysis showed that there were only 23 nuoleotides in difference between this fragment and pomine GHRH receptor gene, and its homology was 98%. However, both GHRH receptor genes were constituted by 423 amino acids with the sequence homology of 96%. [ Conclusion] This study provides theoretical basis for further studies on the dwarf mechanism of Wuzhishan miniature pig.
文摘[ Objective] The study aimed to lay a foundation for studying immune recognition mechanism in xenotransplantation. [ Method] SLA- DQA gene of Hainan Wuzhishan miniature pig was cloned by RT-PCR and sequenced. J-Result] The SLA-DQA gene sequence of Wuzhishan minia- ture pig contained 768 nucleotides, among which 1 -765 were the complete open reading frame, encoding 255 amino acids. SLA-DQA cDNA se- quence and its deduced amino acid sequence of Wuzhishan miniature pig were most similar to those of the other miniature pigs in China. When compared with corresponding cDNA sequences and the amino acid sequences in other species, the homology with human was obviously higher than that with mouse. [ Conclusion] As the Chinese pig breed suitable for xenotransplantation, Wuzhishan miniature pig has significant importance to ser- ial studies on immunogenetics characteristics.
文摘To elucidate the structure of SLA-3 alleles on inbred line of Wuzhishan pig （WZSP） population, we examined the partial exon 1, completed exon 2, and partial exon 3 of SLA-3 loci using the reverse transcription-polymerase chain reaction （RT- PCR） and the sequencing-based method in 32 WZSPs. According to pedigree and amplification results, PCR products of 8 WZSPs were selected to clone and sequence. Nine different nucleotide sequences were obtained. After comparing the DNA and protein sequences of the WZSPs SLA-3 alleles with the published GenBank SLA sequences, it was found that the SLA-3 alleles in WZSPs were all novel, but there were very few variations among them. Comparision of SLA-3 and HLA-A protein sequences indicated that there was more sequence homology. Meanwhile, the construction of a phylogenetic tree using the nucleotide sequences of 23 SLA-3 alleles and 1 HLA-A allele represented that the WZSP population owns its unique genetics resource. In this study, the alleles of SLA-3 on WZSP group were successfully detected and analyzed, which provided the firm basis on the genotype of SLA-3 for breeding specific haplotypes WZSPs.
基金Supported by the Innovative Research Team Project of Natural Science Foundation of Hainan Province(2018CXTD345)Local Science Foundation of National Natural Science Foundation(31560696)+1 种基金Natural Science Foundation of Hainan Province(20153080)Special Fund for the Development of Local Science and Technology Guided by Central Government(ZY2019HN01)
文摘In order to obtain the TLR 4 gene sequence of Hainan Wuzhishan pig and analyze its molecular structure characteristics,its genomic sequence was cloned and sequenced by PCR and subjected to related bioinformatics analysis with the genomic sequence of the TLR 4 gene of pig in GenBank as a reference sequence.The full-length DNA sequence of Wuzhishan pig TLR 4 gene was 10 435 bp in length,of which CDS was 2 526 bp in length and encoded 841 amino acids.Compared with the common pig TLR 4 gene sequence published by GenBank,there were 38 mutations,of which two mutations were in the CDS region.The results of homology analysis showed that the amino acid sequence of the TLR 4 gene of Wuzhishan pig shared 99.9%,80.7%,79.9%,74.8%,74.7%,73.1%,73.0%,72.7%,62.5% and 43.4% homology with those of common pig,cattle,sheep,dog,horse,macaque,human,chimpanzee,mouse and chicken,respectively.The protein encoded by the TLR 4 of Wuzhishan pig belonged to secreted protein and transmembrane protein.It was hydrophilic,and had 8 glycosylation modification sites,17 serines (ser),7 threonines (Thr) and 8 tyrosine (TYR) phosphorylation sites.The amino acid phylogenetic tree analysis showed that the TLR 4 genes of different species are highly conserved during evolution.This result lays a foundation for further study of the function of TLR 4 gene.
文摘As the protected areas of land and coastal environment, nature reserves are designed to address how to reconcile the conservation of biodiversity, the quest for economic and social development and the maintenance of cultural values. This paper establishes a framework for nature reserve development that seeks to incorporate ecotourism into its strategies. The overall purpose was to identify the information needs required for a comprehensive nature reserve that incorporates ecotourism related values. It also illustrates the utility of this framework in the context of the Wuzhishan Mountain Region of China. A literature review, the first phase of a visionary strategy and a subsequent gap analysis for available management information were undertaken in order to achieve this paper＇s purpose. Finally, recommendations axe pre-sented for integrating ecotourism into nature reserve development in the Wuzhishan Mountain Region.
基金funded by the national key basic research development program (2014 cb440905)key project of national natural science funds (41430315)
文摘1 Introduction The Wuzhishan lead-zinc ore-concentrated area in Puding is located in the east of the Sichuan,Guizhou and Yunnan lead-zinc metallogenic domain,with the Youjiang-Nanpan River metallogenic province to the
