AIM To develop methods of articular cartilage preparation for X-ray-electron probe microanalysis and to study its elements content in experimental osteoarthrosis.METHODS Twenty dogs aged 2-8 years were divided in rese...AIM To develop methods of articular cartilage preparation for X-ray-electron probe microanalysis and to study its elements content in experimental osteoarthrosis.METHODS Twenty dogs aged 2-8 years were divided in research(aged 2 years, induction of osteoarthrosis-IOA) and intact group. Intact group included three subgroups(aged 2, 5 and 8 years). Samples of cartilage after araldite saturation and pouring were partially cut into semithin sections stained with methylene blue and with methylene blue-basic fuchsin. Their smooth surfaces were investigated by X-ray-electron probe microanalysis. Spatial distribution of sulfur, calcium and phosphorus and their concentrations(weight %) were investigated.RESULTS X-ray electron probe microanalysis revealed non-uniformsulfur distribution in cartilage of intact animals: Its content increases from superficial zone to deep one, this regularity was preserved in animals with IOA. Differences of IOA with spontaneous chondropathy were revealed. Spontaneous aging was characterized by calcium and phosphorus storage in deep and calcified zones and compensatory increase of sulfated glycosaminoglycans in intermediate and deep cartilage zones as evidenced by the metachromatic reaction and microanalysis data. Unlike spontaneous chondropathy connected with aging in experimentally stimulated osteoarthrosis more intensive storage of calcium but minor phosphorus in intermediate zone were marked. In IOA the calcified cartilage thinning and osteoclastic resorption are apparent with few changes of elements composition; the only difference from control is minority phosphorus content.CONCLUSION The obtained results demonstrate specific tricks of X-ray electron probe microanalysis and its possibility in the research of mechanisms of articular cartilage alterations in osteoarthrosis.展开更多
目的评估X线片在钛合金笼植入治疗犬股骨头缺血性坏死中的作用效果。方法20只普通级健康成年雄性杂交犬,根据实验目的分为2组:对照组(n=10)通过液氮冷冻浇灌造成犬股骨头缺血性坏死模型,进行后期维护,预防伤口感染;实验组(n=10)造成股...目的评估X线片在钛合金笼植入治疗犬股骨头缺血性坏死中的作用效果。方法20只普通级健康成年雄性杂交犬,根据实验目的分为2组:对照组(n=10)通过液氮冷冻浇灌造成犬股骨头缺血性坏死模型,进行后期维护,预防伤口感染;实验组(n=10)造成股骨头缺血性坏死后进行钛合金笼植入治疗,并做好伤口护理。每天观察健康状况、伤口愈合和术后并发症。通过高级骨分析仪分析股骨头的骨矿物质密度和小梁厚度。通过使用Image-Pro Plus 6.0获得切片组织的股骨头宽度和小梁面积百分比。Masson三色染色和CD31免疫组织化学染色分析新骨形成和股骨头微血管密度。通过X线片和组织学分析对犬软骨和股骨头缺损进行评估。术后12周观察比较犬跛行状态。结果实验组较对照组骨矿物质密度和股骨头小梁厚度增大,差异均有统计学意义(P<0.05)。实验组较对照组的小梁面积百分比和小梁宽度升高,差异均有统计学意义(P<0.05)。X线片显示,对照组和实验组重建后12周时犬股骨头的术后变化。在对照组中观察到关节塌陷,实验组中股骨头的轮廓保存完好。实验组较对照组的新骨形成面积和微血管密度升高,差异均有统计学意义(P<0.05)。实验组较对照组放射学评分和组织学评分升高,差异均有统计学意义(P<0.05)。实验组较对照组犬跛行严重程度降低,差异有统计学意义(P<0.05)。结论将钛合金笼植入坏死的股骨头并没有改变股骨头的形状,通过X线片可以发现再生的海绵骨可长入笼内,重建、修复、置换坏死的骨组织,形成海绵骨网络,钛合金笼为血管内皮生长因子诱导材料的植入提供了空间。展开更多
基金Supported by The RF Ministry of Health within government-mandated program for FSBI Russian Ilizarov Scientific Center "Restorative Traumatology and Orthopaedics"(RISC"RTO") for Scientific Research,No.01201155770
文摘AIM To develop methods of articular cartilage preparation for X-ray-electron probe microanalysis and to study its elements content in experimental osteoarthrosis.METHODS Twenty dogs aged 2-8 years were divided in research(aged 2 years, induction of osteoarthrosis-IOA) and intact group. Intact group included three subgroups(aged 2, 5 and 8 years). Samples of cartilage after araldite saturation and pouring were partially cut into semithin sections stained with methylene blue and with methylene blue-basic fuchsin. Their smooth surfaces were investigated by X-ray-electron probe microanalysis. Spatial distribution of sulfur, calcium and phosphorus and their concentrations(weight %) were investigated.RESULTS X-ray electron probe microanalysis revealed non-uniformsulfur distribution in cartilage of intact animals: Its content increases from superficial zone to deep one, this regularity was preserved in animals with IOA. Differences of IOA with spontaneous chondropathy were revealed. Spontaneous aging was characterized by calcium and phosphorus storage in deep and calcified zones and compensatory increase of sulfated glycosaminoglycans in intermediate and deep cartilage zones as evidenced by the metachromatic reaction and microanalysis data. Unlike spontaneous chondropathy connected with aging in experimentally stimulated osteoarthrosis more intensive storage of calcium but minor phosphorus in intermediate zone were marked. In IOA the calcified cartilage thinning and osteoclastic resorption are apparent with few changes of elements composition; the only difference from control is minority phosphorus content.CONCLUSION The obtained results demonstrate specific tricks of X-ray electron probe microanalysis and its possibility in the research of mechanisms of articular cartilage alterations in osteoarthrosis.
文摘目的评估X线片在钛合金笼植入治疗犬股骨头缺血性坏死中的作用效果。方法20只普通级健康成年雄性杂交犬,根据实验目的分为2组:对照组(n=10)通过液氮冷冻浇灌造成犬股骨头缺血性坏死模型,进行后期维护,预防伤口感染;实验组(n=10)造成股骨头缺血性坏死后进行钛合金笼植入治疗,并做好伤口护理。每天观察健康状况、伤口愈合和术后并发症。通过高级骨分析仪分析股骨头的骨矿物质密度和小梁厚度。通过使用Image-Pro Plus 6.0获得切片组织的股骨头宽度和小梁面积百分比。Masson三色染色和CD31免疫组织化学染色分析新骨形成和股骨头微血管密度。通过X线片和组织学分析对犬软骨和股骨头缺损进行评估。术后12周观察比较犬跛行状态。结果实验组较对照组骨矿物质密度和股骨头小梁厚度增大,差异均有统计学意义(P<0.05)。实验组较对照组的小梁面积百分比和小梁宽度升高,差异均有统计学意义(P<0.05)。X线片显示,对照组和实验组重建后12周时犬股骨头的术后变化。在对照组中观察到关节塌陷,实验组中股骨头的轮廓保存完好。实验组较对照组的新骨形成面积和微血管密度升高,差异均有统计学意义(P<0.05)。实验组较对照组放射学评分和组织学评分升高,差异均有统计学意义(P<0.05)。实验组较对照组犬跛行严重程度降低,差异有统计学意义(P<0.05)。结论将钛合金笼植入坏死的股骨头并没有改变股骨头的形状,通过X线片可以发现再生的海绵骨可长入笼内,重建、修复、置换坏死的骨组织,形成海绵骨网络,钛合金笼为血管内皮生长因子诱导材料的植入提供了空间。
