X染色质体在正常人体细胞中出现概率的研究

本文采用X染色质的鉴别方法 ,研究了部分人群正常体细胞中X染色质体的出现概率 .结果表明 三个实验群体的X染色质体的概率分别为 35 .2 1± 6 .6 2 %、33 .0 4± 9.33 %、2 6 .84± 8.5 8% ,它们之间差异不显 著 (P >0 .0 5 ) .说明X染色质体在不同个体的相同组织中出现的情况有异 。

2种X染色质体标本片制备方法的探讨

探讨2种较好的制备人类Ⅹ染色质体标本的方法。取正常女性口腔黏膜细胞涂片，分别采用硫堇染色和甲苯胺蓝染色，油镜下观察。硫堇染色法中，染液配制耗时长，容易受温度影响．染液易结晶．且染色标本片易出现硫堇结晶形成的杂质从而影响观察，染色标本片杂质较多，Ⅹ染色质体检出率为20．3％-42．2％；甲苯胺蓝染色法中，染液配制简单，耗时短，且不易受环境温度影响，染色标本片镜下标本清晰，杂质少。x染色质体检出率为19．8％-43．6％。甲苯胺蓝染液染色观察Ⅹ染色质体耗时短，成本低，操作简单，优于硫堇染色法。

Possible mechanism for hepatitis B virus X gene to induce apoptosis of hepatocytes

AIM： To investigate the possible mechanism for HBV X gene to induce apoptosis of hepatocyte HL-7702 cells.METHODS： HBV X gene eukaryon expression vector pcDNA3-X was established and transfected into HL-7702 cells by lipid-mediated transfection, including transient and stable transfection. Positive clones were screened by incubating in the selective medium with 600 μg/mL G418 and named HL-7702/HBV-encoded X protein （HBx） cells. The expressions of Fas/FasL, Bax/Bcl-2, and c-myc mRNA were measured by semi-quantitative RT-PCR in HL-7702/HBx and control group, respectively.RESULTS： RT-PCR analysis confirmed that HBV X gene was transfected into HL-7702 cells successfully. By semiquantitative RT-PCR analysis, Bax and c-myc mRNA levels in HL-7702/HBx cells of transient transfection were significantly higher than those in control, FasL and c-myc mRNA levels in HL-7702/HBx cells of stable transfection were significantly higher than those in control, whereas the Bcl-2 mRNA levels in HL-7702/HBx cells of transient and stable transfection were significantly lower than thosein control.CONCLUSION： HBV X gene may promote the apoptosis of hepatocytes by regulating the expressions of Fas/FasL, Bax/Bcl-2, and c-myc gene in a dose-dependent manner.