雷帕霉素支架联合X线外照射预防支架置入后血管再狭窄的实验研究

目的:观察雷帕霉素支架、X射线照射及两者联合对支架置入术后血管内膜增生的影响,找出抑制内膜增生的理想方法及评价其安全性。方法:建立比格犬动脉粥样硬化血管支架置入的动物模型,随机分为对照组(仅行常规支架置入)、药物支架组、放射组及联合组,取实验动物的离体血管及空腹静脉血,分别检测其平均管腔面积、血管新生内膜平均增生面积、平滑肌细胞凋亡率及放疗前后静脉血常规。结果:血管平均厚度及血管新生内膜平均增生面积对照组最厚,联合组最低,放射组和药物支架组差异无统计学意义(P>0.05)。对照组凋亡相关蛋白Survivin和Bcl-2表达最高,Caspase-9表达最低;联合组凋亡相关蛋白Survivin和Bcl-2表达最低,Caspase-9表达最高;放射组和药物支架组凋亡相关蛋白表达差异无统计学意义(P>0.05)。放疗后第8天,放疗组与联合组的白细较前降低,但两者之间差异无统计学意义(P>0.05),余各项指标放疗前后无明显变化。结论:与雷帕霉素支架和X线照射相比较,两者联合应用更能促进血管内皮细胞的凋亡,从而更好地抑制血管内膜的增生,且未显示有更多的副作用及并发症。

放射性直肠炎治疗方案优选初探

目的探讨放射性直肠炎治疗较优治疗方案。方法对68例放射性直肠炎患者,根据是否应用激素治疗分为非激素治疗组和激素治疗组。非激素组41例,采用谷胺肠参胶囊+思密达治疗;激素组27例,谷胺肠参胶囊+思密达+地塞米松治疗。结果非激素组治愈率44%(18/41),好转率44%(18/41),无效12%(5/41);激素组治愈率63%(17/27),好转率37%(8/27),两组比较差异无统计学意义(P=0.067)。在两组临床分度Ⅱ度的亚组间比较,非激素组Ⅱ度治愈率33.3%(10/33),好转率53.3%(16/30),无效13.3%(4/30);激素组Ⅱ度治愈率61.9%(13/21),好转率38.1%(8/21),激素组疗效优于非激素组。结论对放射性直肠炎治疗,临床分度Ⅰ度者可以不用激素,而临床分度Ⅱ度者,应用激素可能获得较高的疗效。提示有必要开展大宗病例前瞻性研究,以规范放射性直肠炎治疗。

106例放射性肺炎相关因素分析

目的回顾性分析肺癌放射治疗后并发放射性肺炎的发生率,了解发生的相关因素。方法自1995年1月～2004年12月期间我科收治的793例肺癌病人。采有钴60或直线加速器6-10MVX线外照射,常规分割,DT4600CGY-7200CGY23～36F。照射野面积大于120cm2547例。剂量大于5000CGY的680例。结果放射性肺炎发生率为13.4%。照射野面积大于120cm2,剂量大于5000CGY的放射性肺炎发生率分别为16.2%和14.6%。结论放射治疗引起的放射损伤应引起高度重视,照射野面积大,剂量高,放射性肺炎的发生率高。

肿瘤骨转移的放疗止痛疗效观察

恶性肿瘤骨转移患者不同剂量60 Co或 6MVX线外照射治疗后 ,CR6 4.1% ,PR19.4% ,MR或NR占16 .1%。大分割少分次缓解期最短 ,而常规分割缓解期最长。 3Gy/次 ,5次 /周 ,总量 30Gy剂量组缓解期居中。统计学分析有显著差异 (P <0 .0 5 )。认为放疗止痛效果肯定 。

Characterization of nano-Ag/PVP composites synthesized via ultra-violet irradiation

Nano-silver/polyvinylpyrrolidone(PVP)composite materials were successfully synthesized bi-insitu from silver nitrate solution with N-vinyl pyrrolidone (NVP) monomer,containing neither initiator nor reductant, in ultraviolet irradiation conditions.The resultant Ag/PVP nanocomposites were characterized by infrared spectroscopy (FT-IR), high resolution transmission electron microscopy (HRTEM), X-ray photoelectron spectroscopy (XPS), and X-ray diffraction (XRD).TEM show that nano silver particles are homogeneously dispersed in PVP polymer matrix, and the mean size of spherical silver particles is about 5 nm.The spectroscopy of XPS and FTIR showed that there is an interaction between nano silver not only with carbonyl oxygen but also with the nitrogen group within the NVP molecule through the p-π conjugation effect in the nano-silver/PVP composites system.

The impact of X-rays on the expressions of miR-130a/miR-25 and its potential role in the enhanced metastasis of A549 cell lines in vitro induced by X-rays

We aimed to investigate the impact of X-rays on miR-130a and miR-25 expressions of A549 cell lines and to understand the mechanism of miR-130a and miR-25 on the regulation of invasion and metastasis of A549 cell lines. Human adenocarcinoma cells of the line A549 were cultured and radiated by X-rays at the absorbed doses of 2 and 4, respectively by linear accelerator. Transwell invasion and migration assay were employed to exam the metastasis ability of A549 cells post X-rays irradiation. Both the miRNA and mRNA were extracted from A549 cells at different time points post radiation. The expressions of miR-130a and miR-25, as well as the expressions of VEGF and CCR-7 mRNAs, were detected in A549 cells with 2 and 4 Gy X-rays radiation, respectively by real time PCR. Results were statistically analyzed by SAS 8.2 software, which showed that the invasiveness of A549 cells post 2 and 4 Gy X-rays increased significantly compared with that of untreated A549 cells (the migration cell numbers are 20, 48 and 62 in Group 0, 2 and 4 Gy X-rays, respectively). Furthermore, the expressions of miR-130a and miR-25 also increased significantly at the time-points of 1, 2, 4, and 8 h post radiation, and began to decrease to the control level at 12 h post radiation. VEGF and CCR-7 mRNAs were detected to be up-regulated 18 h post radiation and remained at a high level till 72 h post radiation. The expression of VEGF mRNA has a close correlation with that of CCR-7 mRNA, and the expression of miR-130a also has a correlation with that of VEGF and CCR-7mRNAs. It is concluded that the metastasis of A549 cells in vitro could be promoted by X-rays, and miR-130a might play a role in the metastasis of A549 cells via regulating the expressions of VEGF and CCR-7 mRNAs.

Role of VEGF-A/C and CCR-7 in the enhanced metastasis of A549 cells induced by 2 and 4 Gy X-rays in vitro and in vivo

Radiotherapy is a common strategy in treating lung cancer.Accumulating evidence suggested that radiotherapy has the potential to promote the metastasis and invasion of carcinoma cells.In this study,we aimed to testify the role of vascular endothelial growth factor(VEGF)and C-C chemokine receptor-7(CCR-7)in the metastasis of human adenocarcinoma A549 cells in vivo and in vitro.Nude mice were injected with A549 cells irradiated by 0,2 and 4 Gy X-rays,respectively.Quantitative detections of VEGF-A/C and CCR-7 mRNA from lung sample were performed by real-time RT-PCR.Small interfering RNA(siRNA)transfection technology was further used to testify the role of the genes in the metastasis of A549 cells.VEGF and CCR-7mRNA could only be detected 10 weeks post injection in vivo when visible tumor foci scattered in lung.In addition,VEGF-A/C mRNA expressed significantly higher in mice injected with A549 cells irradiated by 2 and 4 X-rays than those with 0 Gy X-rays irradiation.On the other hand,A549 cells with or without X-rays irradiation transfected with VEGF siRAN and CCR-7 siRNA showed a dramatic decrease of invasiveness compared to normal A549 cells with or without irradiation.The migration indexes were?0.7,?0.48,?0.34 and?0.32 for A549 cells with CCR-7 siRNA,VEGF siRNA,X-rays combined CCR-7 siRNA and X-rays combined VEGF-siRNA respectively.These results demonstrated that X-rays could promote the metastasis of A549 cells,and VEGF-A/C and CCR-7 mRNA were closely related to the metastasis of A549 cells in vivo and in vitro.