WT5”BZ] To study the molecular mechanism of the stimulatory effect of low dose radiation(LDR) on T cell activation. [WT5”BX]Methods.[WT5”BZ] Thymocytes from Kunming mice exposed to whole body irradiation(WBI) with ...WT5”BZ] To study the molecular mechanism of the stimulatory effect of low dose radiation(LDR) on T cell activation. [WT5”BX]Methods.[WT5”BZ] Thymocytes from Kunming mice exposed to whole body irradiation(WBI) with different doses of X rays were analyzed for the changes in signal molecules of the phospholipase C phosphatidylinositol biphosphate(PLC IP2) and G protein adenylate cyclase(AC) pathways. [WT5”BX]Results.[WT5”BZ]It was found that[Ca 2+ ] i increased in response to doses within 0 2 Gy which was most marked after 0 075 Gy and the increase was accentuated in the presence of Con A. The changes in CD3 and calcineurin(CN) expression of the thymocytes followed the same pattern as the alterations in [Ca 2+ ] i after LDR. The expression of α,β1 and β2 isoforms of protein kinase C(PKC) was all up regulated after 0 075 Gy with the increase in PKC β1 expression being most marked. The cAMP/cGMP ratio and PKA activity of the thymocytes was lowered after low dose radiation and increased after doses above 0 5 Gy in a dose dependent manner, thus giving rise to J shaped dose response curves. The Ca antagonist TMB 8 and cAMP stimulant cholera toxin suppressed the augmented thymocyte proliferation induced by LDR. [WT5”BX]Conclusion.[WT5”BZ]Data presented in the present paper suggest that activation of the PLC PIP2 signal pathway and suppression of the AC cAMP signal pathway are involved in the stimulation of the thymocytes following WBI with low dose X rays.展开更多
A special cloth for keeping LiF(Mg,Cu,P)TL dosimetry elements is worn by examinees. The exposures of 128 examinees .received upper G.I.T (gastro-intestinal tram) X-ray examination are measured. The reference point of ...A special cloth for keeping LiF(Mg,Cu,P)TL dosimetry elements is worn by examinees. The exposures of 128 examinees .received upper G.I.T (gastro-intestinal tram) X-ray examination are measured. The reference point of the maximum body surface exposure given is at the middle of stomach. The average of this point is (4.97±1.94) × 10-4C.kg-1 person-1 examination-1 and (1.33±0.28)×10-4C.kg-1.min-1.展开更多
Plutonic and volcanic rocks were analyzed by INAA （Instrumental Neutron Activation Analysis） ana XRF （X-ray flurescence） in their major components for its classification. The samples proceed from the sites/regions...Plutonic and volcanic rocks were analyzed by INAA （Instrumental Neutron Activation Analysis） ana XRF （X-ray flurescence） in their major components for its classification. The samples proceed from the sites/regions corresponding to the Precambrian to Paleozoic/Postpaleozoic magmatism in the eastern and western Paraguay. The analysed elements were Si, A1, Na and K and thus the SiO2, AI/O3, Na2O and K2O content determined. The activation were performed in an annular 25Ci Am-Be neutron source with total flux of 5×10^7n.s^-1. The reactions used were （1） ^28Si （n, p） ^27A1, （2） ^27A1 （n, p） ^27Mg; （3） ^23Na （n, γ） ^24Na, the fourth element of interest, potassium, was analysed by XRF. The procedure overall uncertainties were checked as per the analysis of three compositae samples of SiO2： ＋ A12O3 ＋ NaHCO3 ＋ K2CO3 prepared as standards in convenient proportions. The determination of these parameters according to the last recommendations of the IUGS （International Union of Geosciences）, permits the classification of the rocks in the categories acid, intermediate, basic and ultrabasie, as well as the identification of the rock family and in certain cases, the norm and their magmatic provenance.展开更多
基金This work was supported by a grant from NSFC (No.39570188)
文摘WT5”BZ] To study the molecular mechanism of the stimulatory effect of low dose radiation(LDR) on T cell activation. [WT5”BX]Methods.[WT5”BZ] Thymocytes from Kunming mice exposed to whole body irradiation(WBI) with different doses of X rays were analyzed for the changes in signal molecules of the phospholipase C phosphatidylinositol biphosphate(PLC IP2) and G protein adenylate cyclase(AC) pathways. [WT5”BX]Results.[WT5”BZ]It was found that[Ca 2+ ] i increased in response to doses within 0 2 Gy which was most marked after 0 075 Gy and the increase was accentuated in the presence of Con A. The changes in CD3 and calcineurin(CN) expression of the thymocytes followed the same pattern as the alterations in [Ca 2+ ] i after LDR. The expression of α,β1 and β2 isoforms of protein kinase C(PKC) was all up regulated after 0 075 Gy with the increase in PKC β1 expression being most marked. The cAMP/cGMP ratio and PKA activity of the thymocytes was lowered after low dose radiation and increased after doses above 0 5 Gy in a dose dependent manner, thus giving rise to J shaped dose response curves. The Ca antagonist TMB 8 and cAMP stimulant cholera toxin suppressed the augmented thymocyte proliferation induced by LDR. [WT5”BX]Conclusion.[WT5”BZ]Data presented in the present paper suggest that activation of the PLC PIP2 signal pathway and suppression of the AC cAMP signal pathway are involved in the stimulation of the thymocytes following WBI with low dose X rays.
文摘A special cloth for keeping LiF(Mg,Cu,P)TL dosimetry elements is worn by examinees. The exposures of 128 examinees .received upper G.I.T (gastro-intestinal tram) X-ray examination are measured. The reference point of the maximum body surface exposure given is at the middle of stomach. The average of this point is (4.97±1.94) × 10-4C.kg-1 person-1 examination-1 and (1.33±0.28)×10-4C.kg-1.min-1.
文摘Plutonic and volcanic rocks were analyzed by INAA （Instrumental Neutron Activation Analysis） ana XRF （X-ray flurescence） in their major components for its classification. The samples proceed from the sites/regions corresponding to the Precambrian to Paleozoic/Postpaleozoic magmatism in the eastern and western Paraguay. The analysed elements were Si, A1, Na and K and thus the SiO2, AI/O3, Na2O and K2O content determined. The activation were performed in an annular 25Ci Am-Be neutron source with total flux of 5×10^7n.s^-1. The reactions used were （1） ^28Si （n, p） ^27A1, （2） ^27A1 （n, p） ^27Mg; （3） ^23Na （n, γ） ^24Na, the fourth element of interest, potassium, was analysed by XRF. The procedure overall uncertainties were checked as per the analysis of three compositae samples of SiO2： ＋ A12O3 ＋ NaHCO3 ＋ K2CO3 prepared as standards in convenient proportions. The determination of these parameters according to the last recommendations of the IUGS （International Union of Geosciences）, permits the classification of the rocks in the categories acid, intermediate, basic and ultrabasie, as well as the identification of the rock family and in certain cases, the norm and their magmatic provenance.
