BACKGROUND Pulmonary alveolar proteinosis(PAP)and X-linked agammaglobulinemia(XLA)are rare diseases in children.Many theories infer that immunodeficiency can induce PAP,but these reports are almost all review articles...BACKGROUND Pulmonary alveolar proteinosis(PAP)and X-linked agammaglobulinemia(XLA)are rare diseases in children.Many theories infer that immunodeficiency can induce PAP,but these reports are almost all review articles,and there is little clinical evidence.We report the case of a child with both PAP and XLA.CASE SUMMARY A 4-month-old boy sought medical treatment due to coughing and difficulty in breathing for>2 wk.He had been hospitalized multiple times due to respiratory infections and diarrhea.Chest computed tomography and alveolar lavage fluid showed typical PAP-related manifestations.Genetic testing confirmed that the boy also had XLA.Following total lung alveolar lavage and intravenous immunoglobulin replacement therapy,the boy recovered and was discharged.During the follow-up period,the number of respiratory infections was significantly reduced,and PAP did not recur.CONCLUSION XLA can induce PAP and improving immune function contributes to the prognosis of children with this type of PAP.展开更多
"Synthetic"allopolyploids recreated by interspecific hybridization play an important role in providing novel genomic variation for crop improvement.Such synthetic allopolyploids often undergo rapid genomic s..."Synthetic"allopolyploids recreated by interspecific hybridization play an important role in providing novel genomic variation for crop improvement.Such synthetic allopolyploids often undergo rapid genomic structural variation(SV).However,how such SV arises,is inherited and fixed,and how it affects important traits,has rarely been comprehensively and quantitively studied in advanced generation synthetic lines.A better understanding of these processes will aid breeders in knowing how to best utilize synthetic allopolyploids in breeding programs.Here,we analyzed three genetic mapping populations(735 DH lines)derived from crosses between advanced synthetic and conventional Brassica napus(rapeseed)lines,using whole-genome sequencing to determine genome composition.We observed high tolerance of large structural variants,particularly toward the telomeres,and preferential selection for balanced homoeologous exchanges(duplication/deletion events between the A and C genomes resulting in retention of gene/chromosome dosage between homoeologous chromosome pairs),including stable events involving whole chromosomes("pseudoeuploidy").Given the experimental design(all three populations shared a common parent),we were able to observe that parental SV was regularly inherited,showed genetic hitchhiking effects on segregation,and was one of the major factors inducing adjacent novel and larger SV.Surprisingly,novel SV occurred at low frequencies with no significant impacts on observed fertility and yield-related traits in the advanced generation synthetic lines.However,incorporating genome-wide SV in linkage mapping explained significantly more genetic variance for traits.Our results provide a framework for detecting and understanding the occurrence and inheritance of genomic SV in breeding programs,and support the use of synthetic parents as an important source of novel trait variation.展开更多
This study aims to further promote the inheritance and innovative development of intangible cultural heritage in Yunnan Province,promote the protection,inheritance,integration,and innovation of ethnic culture in Lijia...This study aims to further promote the inheritance and innovative development of intangible cultural heritage in Yunnan Province,promote the protection,inheritance,integration,and innovation of ethnic culture in Lijiang,strengthen the protection and inheritance of Baisha murals in Lijiang,and change the teaching of art design majors in vocational colleges in Yunnan.Given the lack of traditional Chinese culture and local ethnic culture,this article focuses on the teaching of art design majors in Yunnan vocational colleges.It explores the construction model of the cultural inheritance and innovation carrier of Lijiang Baisha murals to meet the spiritual and cultural needs of the local people,and efforts will be made to promote the high-quality development of the Baisha ethnic area in Lijiang.展开更多
AIM:To report a novel splicing mutation in the RPGR gene(encoding retinitis pigmentosa GTPase regulator)in a three-generation Chinese family with X-linked retinitis pigmentosa(XLRP).METHODS:Comprehensive ophthalmic ex...AIM:To report a novel splicing mutation in the RPGR gene(encoding retinitis pigmentosa GTPase regulator)in a three-generation Chinese family with X-linked retinitis pigmentosa(XLRP).METHODS:Comprehensive ophthalmic examinations including best corrected visual acuity,fundus photography,vision field,and pattern-visual evoked potential were performed to identify the disease phenotype of a six-yearold boy from the family(proband).Genomic DNA was extracted from peripheral blood of five available members of the pedigree.Whole-exome sequencing(WES),Sanger sequencing,and pSPL3-based exon trapping were used to investigate the aberrant splicing of RPGR.Human Splice Finder v3.1 and NNSPLICE v0.9 were used for in silico prediction of splice site variants.RESULTS:The proband was diagnosed as having retinitis pigmentosa(RP).He had severe symptoms with early onset.A novel splicing mutation,c.619+1G>C in RPGR was identified in the proband by WES and in four family members by Sanger sequencing.Minigene splicing assays verified that c.619+1G>C in RPGR would result in the formation of a damaging alternative transcript in which the last 91 bp of exon 6 were skipped,leading to the subsequent deletion of 623 correct amino acids(c.529_619del p.Val177Glnfs*16).CONCLUSION:We identify a novel splice donor site mutation causing aberrant splicing of RPGR.Our findings add to the catalog of pathological mutations of RPGR and further emphasize the functional importance of RPGR in RP pathogenesis and its complex clinical phenotypes.展开更多
In the United States,schools offer special education services to children who are diagnosed with a learning or neurodevelopmental disorder and have difficulty meeting their learning goals.Pediatricians may play a key ...In the United States,schools offer special education services to children who are diagnosed with a learning or neurodevelopmental disorder and have difficulty meeting their learning goals.Pediatricians may play a key role in helping children access special education services.The number of children ages 6-21 in the United States receiving special education services increased 10.4%from 2006 to 2021.Children receiving special education services under the autism category increased 242%during the same period.The demand for special education services for children under the developmental delay and other health impaired categories increased by 184%and 83%respectively.Although student enrollment in American schools has remained stable since 2006,the percentage distribution of children receiving special education services nearly tripled for the autism category and quadrupled for the developmental delay category by 2021.Allowable heavy metal residues remain persistent in the American food supply due to food ingredient manufacturing processes.Numerous clinical trial data indicate heavy metal exposures and poor diet are the primary epigenetic factors responsible for the autism and attention deficit hyperactivity disorder epidemics.Dietary heavy metal exposures,especially inorganic mercury and lead may impact gene behavior across generations.In 2021,the United States Congress found heavy metal residues problematic in the American food supply but took no legislative action.Mandatory health warning labels on select foods may be the only way to reduce dietary heavy metal exposures and improve child learning across generations.展开更多
Many populations of the two-spotted spider mite, Tetranychus urticae Koch, have developed high levels of resistance to the pesticide abamectin in China and other countries. This study developed a near-isogenic line to...Many populations of the two-spotted spider mite, Tetranychus urticae Koch, have developed high levels of resistance to the pesticide abamectin in China and other countries. This study developed a near-isogenic line to understand better the inheritance, cross-resistance, and fitness costs associated with abamectin resistance in the field population of T. urticae in China. We introduced the trait that confers extremely high abamectin resistance in a field-collected population of T. urticae into a susceptible laboratory strain(IPP-SS) to generate an abamectin-resistant near-isogenic line(NIL-Aba).This process was carried out through multiple backcrossing to IPP-SS and via parthenogenesis and abamectin screening. Compared with IPP-SS, the NIL-Aba strain had a 25 147-fold resistance to abamectin and a high level of cross-resistance to bifenthrin(288.17-fold), an intermediate level to emamectin benzoate(42.57-fold), and low levels to bifenazate, chlorfenapyr, cyflumetofen, cyenopyrafen, and cyetpyrafen with resistance ranging from 3.18-to 9.31-fold.But it had no cross-resistance to profenofos. The resistance to abamectin in NIL-Aba was autosomal, incompletely dominant, and polygenic. Based on two sex life table parameters, no fitness cost was found in NIL-Aba. Establishing the NIL-Aba strain provides a reliable basis for an in-depth study of abamectin resistance in T. urticae. New information on toxicological characteristics and fitness cost should facilitate the management of abamectin resistance in field populations of T. urticae.展开更多
BACKGROUND Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2) vaccinations have been administered worldwide, with occasional reports of associated neurological complications. Specifically, the impact of vacci...BACKGROUND Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2) vaccinations have been administered worldwide, with occasional reports of associated neurological complications. Specifically, the impact of vaccinations on individuals with Xlinked Charcot-Marie-Tooth disease type 1(CMTX1) is unclear. Patients with CMTX1 can have stroke-like episodes with posterior reversible encephalopathy syndrome on magnetic resonance imaging(MRI), although this is rare.CASE SUMMARY A 39-year-old man was admitted with episodic aphasia and dysphagia for 2 d. He received SARS-CoV-2 vaccination 39 d before admission. Physical examination showed pes cavus and reduced tendon reflexes. Brain MRI showed bilateral, symmetrical, restricted diffusion with T2 hyperintensities in the cerebral hemispheres. Nerve conduction studies revealed peripheral nerve damage. He was diagnosed with Charcot-Marie-Tooth disease, and a hemizygous mutation in the GJB1 gene on the X chromosome, known to be pathogenic for CMTX1, was identified. Initially, we suspected transient ischemic attack or demyelinating leukoencephalopathy. We initiated treatment with antithrombotic therapy and immunotherapy. At 1.5 mo after discharge, brain MRI showed complete resolution of lesions, with no recurrence.CONCLUSION SARS-CoV-2 vaccination could be a predisposing factor for CMTX1 and trigger a sudden presentation.展开更多
Against the background of outstanding traditional Chinese culture and the innovation-drivendevelopment strategy, this study analyzed regional cultures of Yichang where ancient Ba and Chu culturesmet and blended, propo...Against the background of outstanding traditional Chinese culture and the innovation-drivendevelopment strategy, this study analyzed regional cultures of Yichang where ancient Ba and Chu culturesmet and blended, proposed regional architectural designs adapted to modern conditions, fully clarified thedesign concepts of “deeply exploring, highly condensing, making the past serve the present, inheriting andinnovating” through analyzing the design cases in many regional architectural projects. The paper aimedat further inheriting and developing the outstanding traditional Chinese cultures, and provided theoreticalreferences for future designs.展开更多
This paper examines the current status and development dilemma of the children’s folk sports culture in the junction area between Chongqing and Hubei with reference to relevant literature.After the literature review ...This paper examines the current status and development dilemma of the children’s folk sports culture in the junction area between Chongqing and Hubei with reference to relevant literature.After the literature review and summary,the study finds there are several factors restricting the inheritance and development of the children’s folk sports culture in the area,including insufficiency of theoretical analysis,weak public support in cities,blind imitation of modern sports culture,and absence of policy support.To address the above problems,we put forward several suggestions to promote the inheritance and development of children’s folk sports game culture.First,we should focus on the theoretical research and cultural dissemination of children’s folk sports culture.Second,we should improve the basic rules of children’s folk sports games.Third,importance should be attached to government support in developing children’s folk sports game culture.Fourth,we should ensure the continuation and inheritance of children’s folk sports culture via school activities.Finally,we should carry forward children’s folk sports culture and explore ways to develop excellent cultural tourism.展开更多
At the Symposium on Ecological Protection and High-quality Development of the Yellow River Basin, General Secretary Xi Jinping pointed out that "it should deeply explore the time value contained in the Yellow Riv...At the Symposium on Ecological Protection and High-quality Development of the Yellow River Basin, General Secretary Xi Jinping pointed out that "it should deeply explore the time value contained in the Yellow River culture, tell the story of the Yellow River well, and protect, inherit and carry forward the Yellow River culture". Water park is an important carrier of the Yellow River culture transmission. In this paper, the basic characteristics and regional distribution of water parks in the Yellow River basin are analyzed. For the problems faced by cultural heritage of water parks in the Yellow River basin, from three aspects of exploring and integrating the connotation, innovating communication methods, and shaping brand IP, cultural heritage of water parks in the Yellow River basin is discussed. It is beneficial to carry forward the Yellow River culture, and better meet the spiritual and cultural needs of the people.展开更多
The traditional musical instrument of minorities converges the evolution of ethnic group culture and history,and ethnic musical instruments are a core component of minority culture.The traditional instruments of Guang...The traditional musical instrument of minorities converges the evolution of ethnic group culture and history,and ethnic musical instruments are a core component of minority culture.The traditional instruments of Guangxi minorities are rich and varied,and an all-round cultural inheritance and protection mechanism should be used for ethnic musical instruments,so that the performance form of classical musical instruments of minorities can be more widely understood and mastered.Therefore,in this paper,we discuss the specific practice idea of the inheritance and development of classical musical instruments of Guangxi minorities,and facilitate the innovation of development path of ethnic musical instruments by taking the inheritance form of integrating musical instruments with operas.展开更多
It is known that intangible cultural heritage is an important resource of the cultural soft power in a country, and it is also an organic component and representation of national history, spirit and cohesion.Therefore...It is known that intangible cultural heritage is an important resource of the cultural soft power in a country, and it is also an organic component and representation of national history, spirit and cohesion.Therefore, a good inheritance of intangible cultural heritage is of great significance for carrying forward the ethical basis of human culture as well as maintaining its value core. Nowadays, the rapid intelligentization of media has ushered in the era of intellectual media, with characteristics of “massive media, man-machine integration and self-evolution”. In the era of intellectual media, traces of intelligent technologies can be seen everywhere, and the ecology of the inheritance of intangible cultural heritage has been reconstructed. Under this circumstance, the author firstly makes clarification of the advancement of the era, which has given a new logic of intangible cultural heritage inheritance, and various intelligent powers should be developed including intelligent acquisition, processing and judgement ability of content value. Then, the technology empowerment is analyzed, which has made reconstruction of intangible cultural heritage inheritance: while being digitalized,the inheritance of intangible cultural heritage has become systematized in way of “Points, Lines and Planes” at the same time. Therefore, the communication ecology of intangible cultural heritage could be established as a new approach of inheritance via all sorts of intelligent technologies, so as to promote Chinese cultural soft power and enhance our national image in the long run.展开更多
Red music culture is a cultural form created by the people led by the Communist Party of China(CPC)in the process of revolution,construction,and reform.The inheritance and dissemination of this cultural form in univer...Red music culture is a cultural form created by the people led by the Communist Party of China(CPC)in the process of revolution,construction,and reform.The inheritance and dissemination of this cultural form in universities,especially in the context of multiculturalism,has important social and cultural value.This study takes the red music culture in Shanghai as the research carrier,and explores the inheritance path of red music in universities through background investigation,theoretical research,specific practice,and achievement display.The research results show that red music culture can be effectively inherited and promoted by strengthening the construction of the red music culture cloud platform in universities,integrating red music culture resources into the campus environment,and promoting it in Chinese,English,and Japanese on social media.In addition,this study also explores the effective role of red music culture in establishing correct ideals and beliefs among college students.展开更多
Ethnic education is an important way to inherit ethnic culture.Under the background of cultural globalization and cultural diversification,minority cultures are facing the danger of extinction and change.Schools in mi...Ethnic education is an important way to inherit ethnic culture.Under the background of cultural globalization and cultural diversification,minority cultures are facing the danger of extinction and change.Schools in minority areas give full play to their own advantages and become the main places to train students to master ethnic culture in a purposeful,planned,and organized way.However,there are also many problems in school education in the inheritance of national culture,such as the content of school education and national culture cannot be effectively integrated,teachers lack scientific and effective teaching methods,teaching activities content cannot be flexibly used.In this regard,we should excavate the national cultural resources in school education,improve the professional quality and skills of teachers,and enhance the national consciousness of teachers,so as to improve the quality of school education and realize the effective inheritance of national culture.Taking Tibetan areas as an example,this paper analyzes the advantages,existing problems,and solutions of the school in Tibetan cultural traditions.展开更多
Medical institution preparations play an important role in the development of hospitals,being one of the main forms of traditional Chinese medicine clinical characteristic medication,as well as an important carrier fo...Medical institution preparations play an important role in the development of hospitals,being one of the main forms of traditional Chinese medicine clinical characteristic medication,as well as an important carrier for the inheritance and innovation of traditional Chinese medicine.In recent years,the research and development of traditional Chinese medicine preparations has become a hot spot in the research and development of preparations by medical institutions.Traditional Chinese medicine preparations prepared by traditional processes and their entrusted preparation matters have changed from the current licensing management to filing management.The registration model of traditional Chinese medicine preparations has undergone significant changes,emphasizing more on real data and evaluation results from the objective world,respecting traditional medication experience,and emphasizing the inheritance and innovative development of traditional technological experience.In the process of following teachers in the inheritance project of traditional Chinese medicine,we combined the inheritance of traditional Chinese medicine characteristic technology with the research and development practice of traditional Chinese medicine preparations in medical institutions,and focused on the analysis and discussion of technical problems such as prescription screening,dosage form selection,process route design and clinical observation of traditional Chinese medicine preparations,hoping to provide reference for scholars in the same field of traditional Chinese medicine.展开更多
Sentence“bei”(“被”字句)and“bei xx”(被xx)provide syntactic and semantic basis for the generation of the construction“You Bei X Dao(有被x到)”.They are in a continuum and have family similarity.The sentence“bei...Sentence“bei”(“被”字句)and“bei xx”(被xx)provide syntactic and semantic basis for the generation of the construction“You Bei X Dao(有被x到)”.They are in a continuum and have family similarity.The sentence“bei”is a typical member,while“bei xx”is an atypical member.Because the construction“You Bei X Dao”has a single function and less attention,it is at the edge of the continuum and is a marginal member.However,it has certain research value in the inheritance and innovation of“bei”and“bei xx”.展开更多
The Mao Dun Literature Prize is one of the well-known literary awards in China,and in the process of selection over the years,the Mao Dun Literature Prize has developed its own aesthetic orientation.A comparison is ma...The Mao Dun Literature Prize is one of the well-known literary awards in China,and in the process of selection over the years,the Mao Dun Literature Prize has developed its own aesthetic orientation.A comparison is made between the early generation of Xu Mao and His Daughters and the recent award-winning work,A Tale of the Wind,to discover the aesthetic heritage in its flux,which is enlightening for the literary criticism and an orientation for the literary creators.展开更多
From the perspective of narratology, this paper explores the contemporary inheritance of the medical legacy of Zhang Zhongjing's practice medicine in the clinic. Its inheritance mode includes: the enrichment of la...From the perspective of narratology, this paper explores the contemporary inheritance of the medical legacy of Zhang Zhongjing's practice medicine in the clinic. Its inheritance mode includes: the enrichment of language and characters and the narratio of romance, the spatial reductive narratio of cultural landscape, the body expressive narratio of ritual behavior. This makes the legend more concerned with the real society, enhances the regional cultural image, develops the local economy, and confidences to the practices role of carrying forward the response and mission of practicing medicine to save people. Compared with the previous academic circle, which previous focus on the textual research of the dispute over its historical authentication, or focus on the retrospective study of its historical origin, this paper is based on the research perspective of historical inheritance, which is constructive to the living inheritance and protection of legends.展开更多
This editorial,comments on the article by Spartalis et al published in the recent issue of the World Journal of Cardiology.We here provide an outlook on potential ethical concerns related to the future application of ...This editorial,comments on the article by Spartalis et al published in the recent issue of the World Journal of Cardiology.We here provide an outlook on potential ethical concerns related to the future application of gene therapy in the field of inherited arrhythmias.As monogenic diseases with no or few therapeutic options available through standard care,inherited arrhythmias are ideal candidates to gene therapy in their treatment.Patients with inherited arrhythmias typically have a poor quality of life,especially young people engaged in agonistic sports.While genome editing for treatment of inherited arrhythmias still has theoretical application,advances in CRISPR/Cas9 technology now allows the generation of knock-in animal models of the disease.However,clinical translation is somehow expected soon and this make consistent discussing about ethical concerns related to gene editing in inherited arrhythmias.Genomic off-target activity is a known technical issue,but its relationship with ethnical and individual genetical diversity raises concerns about an equitable accessibility.Meanwhile,the costeffectiveness may further limit an equal distribution of gene therapies.The economic burden of gene therapies on healthcare systems is is increasingly recognized as a pressing concern.A growing body of studies are reporting uncertainty in payback periods with intuitive short-term effects for insurance-based healthcare systems,but potential concerns for universal healthcare systems in the long term as well.Altogether,those aspects strongly indicate a need of regulatory entities to manage those issues.展开更多
The authors evaluated 57 parental inbred lines of maize hybrids disseminated in Southwest China for drought tolerance under drought-stressed and well-watered conditions. Multiple regression analyses between drought to...The authors evaluated 57 parental inbred lines of maize hybrids disseminated in Southwest China for drought tolerance under drought-stressed and well-watered conditions. Multiple regression analyses between drought tolerant coefficients of the grain yield per plant and 15 morphological and physiological traits measured from a subset of 12 selected lines, identified traits 1 and 5, which were important for drought tolerance, at the seedling and reproductive stages respectively. Gene effects, combining abilities, and heritabilities of these traits were estimated using generation mean and diallel cross methods. Dominance effect was more important than additive effect for the plant height, anthesis-silking interval (ASI), root weight, and the grain yield per plant, whereas, they were about equal for the leaf emergence rate. The variances of special combining ability (SCA) were about double that of the general combining ability (GCA) for plant height, ASI and grain yield per plant, although they were about equal for leaf emergence rate and root weight. Narrow sense heritabilities of the five traits for the reproductive stage were not high (12.8-29.6%), although broad sense heritabilities for plant height, ASI, and grain yield were as high as 70-85%. A segregating population consisting of 183 F2 plants from the cross N87-1 (drought tolerant) × 9526 (susceptible), was genotyped at 103 SSR loci and the F2:4 families were evaluated under two water regimes. Twelve quantitative trait loci (QTLs) (two for plant height, five for ASI, four for root biomass, and one for grain yield) were identified, most of which had overdominant gene action. Some chromosomal regions, such as those linked to markers umcl051 (bin 4.08), umc2881 (bin 4.03), and phi034 (bin 7.02), had overlapping QTLs.展开更多
基金Supported by Sanitation Research Project of Kunming Municipal Health Commission,No.2020-06-01-119.
文摘BACKGROUND Pulmonary alveolar proteinosis(PAP)and X-linked agammaglobulinemia(XLA)are rare diseases in children.Many theories infer that immunodeficiency can induce PAP,but these reports are almost all review articles,and there is little clinical evidence.We report the case of a child with both PAP and XLA.CASE SUMMARY A 4-month-old boy sought medical treatment due to coughing and difficulty in breathing for>2 wk.He had been hospitalized multiple times due to respiratory infections and diarrhea.Chest computed tomography and alveolar lavage fluid showed typical PAP-related manifestations.Genetic testing confirmed that the boy also had XLA.Following total lung alveolar lavage and intravenous immunoglobulin replacement therapy,the boy recovered and was discharged.During the follow-up period,the number of respiratory infections was significantly reduced,and PAP did not recur.CONCLUSION XLA can induce PAP and improving immune function contributes to the prognosis of children with this type of PAP.
基金supported by the National Natural Science Foundation of China(NSFC,31970564,32000397,32171982)the Fundamental Research Funds for the Central Universities(2662023PY004)。
文摘"Synthetic"allopolyploids recreated by interspecific hybridization play an important role in providing novel genomic variation for crop improvement.Such synthetic allopolyploids often undergo rapid genomic structural variation(SV).However,how such SV arises,is inherited and fixed,and how it affects important traits,has rarely been comprehensively and quantitively studied in advanced generation synthetic lines.A better understanding of these processes will aid breeders in knowing how to best utilize synthetic allopolyploids in breeding programs.Here,we analyzed three genetic mapping populations(735 DH lines)derived from crosses between advanced synthetic and conventional Brassica napus(rapeseed)lines,using whole-genome sequencing to determine genome composition.We observed high tolerance of large structural variants,particularly toward the telomeres,and preferential selection for balanced homoeologous exchanges(duplication/deletion events between the A and C genomes resulting in retention of gene/chromosome dosage between homoeologous chromosome pairs),including stable events involving whole chromosomes("pseudoeuploidy").Given the experimental design(all three populations shared a common parent),we were able to observe that parental SV was regularly inherited,showed genetic hitchhiking effects on segregation,and was one of the major factors inducing adjacent novel and larger SV.Surprisingly,novel SV occurred at low frequencies with no significant impacts on observed fertility and yield-related traits in the advanced generation synthetic lines.However,incorporating genome-wide SV in linkage mapping explained significantly more genetic variance for traits.Our results provide a framework for detecting and understanding the occurrence and inheritance of genomic SV in breeding programs,and support the use of synthetic parents as an important source of novel trait variation.
文摘This study aims to further promote the inheritance and innovative development of intangible cultural heritage in Yunnan Province,promote the protection,inheritance,integration,and innovation of ethnic culture in Lijiang,strengthen the protection and inheritance of Baisha murals in Lijiang,and change the teaching of art design majors in vocational colleges in Yunnan.Given the lack of traditional Chinese culture and local ethnic culture,this article focuses on the teaching of art design majors in Yunnan vocational colleges.It explores the construction model of the cultural inheritance and innovation carrier of Lijiang Baisha murals to meet the spiritual and cultural needs of the local people,and efforts will be made to promote the high-quality development of the Baisha ethnic area in Lijiang.
基金Supported by National Natural Science Foundation of China(No.31751003)Natural Science Foundation of Zhejiang Province(No.LY20H120009)+1 种基金Health Commission of Zhejiang Province(No.2022KY168)Beijing Bethune Charitable Foundation(No.BJ-GY2021013J).
文摘AIM:To report a novel splicing mutation in the RPGR gene(encoding retinitis pigmentosa GTPase regulator)in a three-generation Chinese family with X-linked retinitis pigmentosa(XLRP).METHODS:Comprehensive ophthalmic examinations including best corrected visual acuity,fundus photography,vision field,and pattern-visual evoked potential were performed to identify the disease phenotype of a six-yearold boy from the family(proband).Genomic DNA was extracted from peripheral blood of five available members of the pedigree.Whole-exome sequencing(WES),Sanger sequencing,and pSPL3-based exon trapping were used to investigate the aberrant splicing of RPGR.Human Splice Finder v3.1 and NNSPLICE v0.9 were used for in silico prediction of splice site variants.RESULTS:The proband was diagnosed as having retinitis pigmentosa(RP).He had severe symptoms with early onset.A novel splicing mutation,c.619+1G>C in RPGR was identified in the proband by WES and in four family members by Sanger sequencing.Minigene splicing assays verified that c.619+1G>C in RPGR would result in the formation of a damaging alternative transcript in which the last 91 bp of exon 6 were skipped,leading to the subsequent deletion of 623 correct amino acids(c.529_619del p.Val177Glnfs*16).CONCLUSION:We identify a novel splice donor site mutation causing aberrant splicing of RPGR.Our findings add to the catalog of pathological mutations of RPGR and further emphasize the functional importance of RPGR in RP pathogenesis and its complex clinical phenotypes.
文摘In the United States,schools offer special education services to children who are diagnosed with a learning or neurodevelopmental disorder and have difficulty meeting their learning goals.Pediatricians may play a key role in helping children access special education services.The number of children ages 6-21 in the United States receiving special education services increased 10.4%from 2006 to 2021.Children receiving special education services under the autism category increased 242%during the same period.The demand for special education services for children under the developmental delay and other health impaired categories increased by 184%and 83%respectively.Although student enrollment in American schools has remained stable since 2006,the percentage distribution of children receiving special education services nearly tripled for the autism category and quadrupled for the developmental delay category by 2021.Allowable heavy metal residues remain persistent in the American food supply due to food ingredient manufacturing processes.Numerous clinical trial data indicate heavy metal exposures and poor diet are the primary epigenetic factors responsible for the autism and attention deficit hyperactivity disorder epidemics.Dietary heavy metal exposures,especially inorganic mercury and lead may impact gene behavior across generations.In 2021,the United States Congress found heavy metal residues problematic in the American food supply but took no legislative action.Mandatory health warning labels on select foods may be the only way to reduce dietary heavy metal exposures and improve child learning across generations.
基金funded by the National Natural Science Foundation of China (32072458)the earmarked fund for China Agriculture Research System (CARS-25)the Beijing Key Laboratory for Pest Control and Sustainable Cultivation of Vegetables, China, and the Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences (CAAS-ASTIPIVFCAAS)。
文摘Many populations of the two-spotted spider mite, Tetranychus urticae Koch, have developed high levels of resistance to the pesticide abamectin in China and other countries. This study developed a near-isogenic line to understand better the inheritance, cross-resistance, and fitness costs associated with abamectin resistance in the field population of T. urticae in China. We introduced the trait that confers extremely high abamectin resistance in a field-collected population of T. urticae into a susceptible laboratory strain(IPP-SS) to generate an abamectin-resistant near-isogenic line(NIL-Aba).This process was carried out through multiple backcrossing to IPP-SS and via parthenogenesis and abamectin screening. Compared with IPP-SS, the NIL-Aba strain had a 25 147-fold resistance to abamectin and a high level of cross-resistance to bifenthrin(288.17-fold), an intermediate level to emamectin benzoate(42.57-fold), and low levels to bifenazate, chlorfenapyr, cyflumetofen, cyenopyrafen, and cyetpyrafen with resistance ranging from 3.18-to 9.31-fold.But it had no cross-resistance to profenofos. The resistance to abamectin in NIL-Aba was autosomal, incompletely dominant, and polygenic. Based on two sex life table parameters, no fitness cost was found in NIL-Aba. Establishing the NIL-Aba strain provides a reliable basis for an in-depth study of abamectin resistance in T. urticae. New information on toxicological characteristics and fitness cost should facilitate the management of abamectin resistance in field populations of T. urticae.
基金Supported by Shenzhen Science and Technology Project,No. SGLH20180628161804465The Clinical Research Project of The First Affiliated Hospital of Shenzhen University,No. 20203357035 and No. 20223357021。
文摘BACKGROUND Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2) vaccinations have been administered worldwide, with occasional reports of associated neurological complications. Specifically, the impact of vaccinations on individuals with Xlinked Charcot-Marie-Tooth disease type 1(CMTX1) is unclear. Patients with CMTX1 can have stroke-like episodes with posterior reversible encephalopathy syndrome on magnetic resonance imaging(MRI), although this is rare.CASE SUMMARY A 39-year-old man was admitted with episodic aphasia and dysphagia for 2 d. He received SARS-CoV-2 vaccination 39 d before admission. Physical examination showed pes cavus and reduced tendon reflexes. Brain MRI showed bilateral, symmetrical, restricted diffusion with T2 hyperintensities in the cerebral hemispheres. Nerve conduction studies revealed peripheral nerve damage. He was diagnosed with Charcot-Marie-Tooth disease, and a hemizygous mutation in the GJB1 gene on the X chromosome, known to be pathogenic for CMTX1, was identified. Initially, we suspected transient ischemic attack or demyelinating leukoencephalopathy. We initiated treatment with antithrombotic therapy and immunotherapy. At 1.5 mo after discharge, brain MRI showed complete resolution of lesions, with no recurrence.CONCLUSION SARS-CoV-2 vaccination could be a predisposing factor for CMTX1 and trigger a sudden presentation.
基金Open Fund of Ba-Chu Art Development&Research Center,Key Base of 2021 Humanities and Social Sciences Researches of Hubei Universities and Colleges(2021KF06)Key Research Program of 2020 Philosophy and Social Sciences OF Hubei Universities and Colleges(20ZD033).
文摘Against the background of outstanding traditional Chinese culture and the innovation-drivendevelopment strategy, this study analyzed regional cultures of Yichang where ancient Ba and Chu culturesmet and blended, proposed regional architectural designs adapted to modern conditions, fully clarified thedesign concepts of “deeply exploring, highly condensing, making the past serve the present, inheriting andinnovating” through analyzing the design cases in many regional architectural projects. The paper aimedat further inheriting and developing the outstanding traditional Chinese cultures, and provided theoreticalreferences for future designs.
文摘This paper examines the current status and development dilemma of the children’s folk sports culture in the junction area between Chongqing and Hubei with reference to relevant literature.After the literature review and summary,the study finds there are several factors restricting the inheritance and development of the children’s folk sports culture in the area,including insufficiency of theoretical analysis,weak public support in cities,blind imitation of modern sports culture,and absence of policy support.To address the above problems,we put forward several suggestions to promote the inheritance and development of children’s folk sports game culture.First,we should focus on the theoretical research and cultural dissemination of children’s folk sports culture.Second,we should improve the basic rules of children’s folk sports games.Third,importance should be attached to government support in developing children’s folk sports game culture.Fourth,we should ensure the continuation and inheritance of children’s folk sports culture via school activities.Finally,we should carry forward children’s folk sports culture and explore ways to develop excellent cultural tourism.
文摘At the Symposium on Ecological Protection and High-quality Development of the Yellow River Basin, General Secretary Xi Jinping pointed out that "it should deeply explore the time value contained in the Yellow River culture, tell the story of the Yellow River well, and protect, inherit and carry forward the Yellow River culture". Water park is an important carrier of the Yellow River culture transmission. In this paper, the basic characteristics and regional distribution of water parks in the Yellow River basin are analyzed. For the problems faced by cultural heritage of water parks in the Yellow River basin, from three aspects of exploring and integrating the connotation, innovating communication methods, and shaping brand IP, cultural heritage of water parks in the Yellow River basin is discussed. It is beneficial to carry forward the Yellow River culture, and better meet the spiritual and cultural needs of the people.
文摘The traditional musical instrument of minorities converges the evolution of ethnic group culture and history,and ethnic musical instruments are a core component of minority culture.The traditional instruments of Guangxi minorities are rich and varied,and an all-round cultural inheritance and protection mechanism should be used for ethnic musical instruments,so that the performance form of classical musical instruments of minorities can be more widely understood and mastered.Therefore,in this paper,we discuss the specific practice idea of the inheritance and development of classical musical instruments of Guangxi minorities,and facilitate the innovation of development path of ethnic musical instruments by taking the inheritance form of integrating musical instruments with operas.
文摘It is known that intangible cultural heritage is an important resource of the cultural soft power in a country, and it is also an organic component and representation of national history, spirit and cohesion.Therefore, a good inheritance of intangible cultural heritage is of great significance for carrying forward the ethical basis of human culture as well as maintaining its value core. Nowadays, the rapid intelligentization of media has ushered in the era of intellectual media, with characteristics of “massive media, man-machine integration and self-evolution”. In the era of intellectual media, traces of intelligent technologies can be seen everywhere, and the ecology of the inheritance of intangible cultural heritage has been reconstructed. Under this circumstance, the author firstly makes clarification of the advancement of the era, which has given a new logic of intangible cultural heritage inheritance, and various intelligent powers should be developed including intelligent acquisition, processing and judgement ability of content value. Then, the technology empowerment is analyzed, which has made reconstruction of intangible cultural heritage inheritance: while being digitalized,the inheritance of intangible cultural heritage has become systematized in way of “Points, Lines and Planes” at the same time. Therefore, the communication ecology of intangible cultural heritage could be established as a new approach of inheritance via all sorts of intelligent technologies, so as to promote Chinese cultural soft power and enhance our national image in the long run.
文摘Red music culture is a cultural form created by the people led by the Communist Party of China(CPC)in the process of revolution,construction,and reform.The inheritance and dissemination of this cultural form in universities,especially in the context of multiculturalism,has important social and cultural value.This study takes the red music culture in Shanghai as the research carrier,and explores the inheritance path of red music in universities through background investigation,theoretical research,specific practice,and achievement display.The research results show that red music culture can be effectively inherited and promoted by strengthening the construction of the red music culture cloud platform in universities,integrating red music culture resources into the campus environment,and promoting it in Chinese,English,and Japanese on social media.In addition,this study also explores the effective role of red music culture in establishing correct ideals and beliefs among college students.
文摘Ethnic education is an important way to inherit ethnic culture.Under the background of cultural globalization and cultural diversification,minority cultures are facing the danger of extinction and change.Schools in minority areas give full play to their own advantages and become the main places to train students to master ethnic culture in a purposeful,planned,and organized way.However,there are also many problems in school education in the inheritance of national culture,such as the content of school education and national culture cannot be effectively integrated,teachers lack scientific and effective teaching methods,teaching activities content cannot be flexibly used.In this regard,we should excavate the national cultural resources in school education,improve the professional quality and skills of teachers,and enhance the national consciousness of teachers,so as to improve the quality of school education and realize the effective inheritance of national culture.Taking Tibetan areas as an example,this paper analyzes the advantages,existing problems,and solutions of the school in Tibetan cultural traditions.
基金Supported by The Third Batch of Traditional Chinese Medicine Famous Doctors Inheritance Implementation Project in Guizhou Province in 2021(QZYYH[2021]106).
文摘Medical institution preparations play an important role in the development of hospitals,being one of the main forms of traditional Chinese medicine clinical characteristic medication,as well as an important carrier for the inheritance and innovation of traditional Chinese medicine.In recent years,the research and development of traditional Chinese medicine preparations has become a hot spot in the research and development of preparations by medical institutions.Traditional Chinese medicine preparations prepared by traditional processes and their entrusted preparation matters have changed from the current licensing management to filing management.The registration model of traditional Chinese medicine preparations has undergone significant changes,emphasizing more on real data and evaluation results from the objective world,respecting traditional medication experience,and emphasizing the inheritance and innovative development of traditional technological experience.In the process of following teachers in the inheritance project of traditional Chinese medicine,we combined the inheritance of traditional Chinese medicine characteristic technology with the research and development practice of traditional Chinese medicine preparations in medical institutions,and focused on the analysis and discussion of technical problems such as prescription screening,dosage form selection,process route design and clinical observation of traditional Chinese medicine preparations,hoping to provide reference for scholars in the same field of traditional Chinese medicine.
基金BLCU supported project for young researchers’program(supported by the Fundamental Research Funds for the Central Universities)(Project No.,22YCX083).
文摘Sentence“bei”(“被”字句)and“bei xx”(被xx)provide syntactic and semantic basis for the generation of the construction“You Bei X Dao(有被x到)”.They are in a continuum and have family similarity.The sentence“bei”is a typical member,while“bei xx”is an atypical member.Because the construction“You Bei X Dao”has a single function and less attention,it is at the edge of the continuum and is a marginal member.However,it has certain research value in the inheritance and innovation of“bei”and“bei xx”.
文摘The Mao Dun Literature Prize is one of the well-known literary awards in China,and in the process of selection over the years,the Mao Dun Literature Prize has developed its own aesthetic orientation.A comparison is made between the early generation of Xu Mao and His Daughters and the recent award-winning work,A Tale of the Wind,to discover the aesthetic heritage in its flux,which is enlightening for the literary criticism and an orientation for the literary creators.
基金Henan Xing Culture Engineering Culture Research Project(2022XWH231)Zhang Zhongjing's Inheritance and Innovation Project(GZY-KJS-2022-039-1,GZY-KJS-2022-043-1,GZY-KJS-2022-045-3)+2 种基金Special Research Project of Traditional Chinese Medicine in Henan Province(2023ZY2194)Research Project of National Museum of Traditional Chinese Medicine(NMTCM-YJ-2022-22)Research Project on Culture and Management of Traditional Chinese Medicine in Henan Province(TCM2020023).
文摘From the perspective of narratology, this paper explores the contemporary inheritance of the medical legacy of Zhang Zhongjing's practice medicine in the clinic. Its inheritance mode includes: the enrichment of language and characters and the narratio of romance, the spatial reductive narratio of cultural landscape, the body expressive narratio of ritual behavior. This makes the legend more concerned with the real society, enhances the regional cultural image, develops the local economy, and confidences to the practices role of carrying forward the response and mission of practicing medicine to save people. Compared with the previous academic circle, which previous focus on the textual research of the dispute over its historical authentication, or focus on the retrospective study of its historical origin, this paper is based on the research perspective of historical inheritance, which is constructive to the living inheritance and protection of legends.
文摘This editorial,comments on the article by Spartalis et al published in the recent issue of the World Journal of Cardiology.We here provide an outlook on potential ethical concerns related to the future application of gene therapy in the field of inherited arrhythmias.As monogenic diseases with no or few therapeutic options available through standard care,inherited arrhythmias are ideal candidates to gene therapy in their treatment.Patients with inherited arrhythmias typically have a poor quality of life,especially young people engaged in agonistic sports.While genome editing for treatment of inherited arrhythmias still has theoretical application,advances in CRISPR/Cas9 technology now allows the generation of knock-in animal models of the disease.However,clinical translation is somehow expected soon and this make consistent discussing about ethical concerns related to gene editing in inherited arrhythmias.Genomic off-target activity is a known technical issue,but its relationship with ethnical and individual genetical diversity raises concerns about an equitable accessibility.Meanwhile,the costeffectiveness may further limit an equal distribution of gene therapies.The economic burden of gene therapies on healthcare systems is is increasingly recognized as a pressing concern.A growing body of studies are reporting uncertainty in payback periods with intuitive short-term effects for insurance-based healthcare systems,but potential concerns for universal healthcare systems in the long term as well.Altogether,those aspects strongly indicate a need of regulatory entities to manage those issues.
基金supported by the Asian Development Bank(RETA 6055,Asian Maize Biotechnology Network),the Rockefeller Foundation(2004 FS 047)the National Natural Science Foundation of China(30571172)the Program for Changjiang Scholars and Innovative Research Team in Universities,China(IRT0453).
文摘The authors evaluated 57 parental inbred lines of maize hybrids disseminated in Southwest China for drought tolerance under drought-stressed and well-watered conditions. Multiple regression analyses between drought tolerant coefficients of the grain yield per plant and 15 morphological and physiological traits measured from a subset of 12 selected lines, identified traits 1 and 5, which were important for drought tolerance, at the seedling and reproductive stages respectively. Gene effects, combining abilities, and heritabilities of these traits were estimated using generation mean and diallel cross methods. Dominance effect was more important than additive effect for the plant height, anthesis-silking interval (ASI), root weight, and the grain yield per plant, whereas, they were about equal for the leaf emergence rate. The variances of special combining ability (SCA) were about double that of the general combining ability (GCA) for plant height, ASI and grain yield per plant, although they were about equal for leaf emergence rate and root weight. Narrow sense heritabilities of the five traits for the reproductive stage were not high (12.8-29.6%), although broad sense heritabilities for plant height, ASI, and grain yield were as high as 70-85%. A segregating population consisting of 183 F2 plants from the cross N87-1 (drought tolerant) × 9526 (susceptible), was genotyped at 103 SSR loci and the F2:4 families were evaluated under two water regimes. Twelve quantitative trait loci (QTLs) (two for plant height, five for ASI, four for root biomass, and one for grain yield) were identified, most of which had overdominant gene action. Some chromosomal regions, such as those linked to markers umcl051 (bin 4.08), umc2881 (bin 4.03), and phi034 (bin 7.02), had overlapping QTLs.