目的探索DNA损伤修复蛋白XPF表达水平影响肾癌细胞顺铂耐药的作用与机制。方法 Western blot法检测不同肾癌细胞株的XPF蛋白水平;采用shRNA干扰技术,筛选稳定低表达XPF的细胞株;采用克隆形成实验及细胞凋亡检测实验,比较XPF低表达细胞株...目的探索DNA损伤修复蛋白XPF表达水平影响肾癌细胞顺铂耐药的作用与机制。方法 Western blot法检测不同肾癌细胞株的XPF蛋白水平;采用shRNA干扰技术,筛选稳定低表达XPF的细胞株;采用克隆形成实验及细胞凋亡检测实验,比较XPF低表达细胞株与XPF正常表达细胞株的顺铂敏感性;通过β-半乳糖苷酶染色法,检测细胞的衰老程度。结果在肾癌细胞786-0、ACHN和Caki-1中,ACHN细胞的XPF表达水平最高,其顺铂耐药程度也最高。shRNA干扰获得稳定低表达XPF的ACHN细胞,顺铂处理后,其克隆形成率低于对照(P<0.05),细胞凋亡率高于对照,衰老细胞比例也显著高于对照(P<0.01)。结论肾癌化疗不敏感可能与其高表达DNA损伤修复蛋白XPF有关,干预并下调肾癌ACHN细胞XPF表达,可增强顺铂的凋亡诱导效应并加速衰老,从而提高其顺铂敏感性。展开更多
To the Editor:Glioma is a highly invasive and lethal heterogeneous disease caused by glial or precursor cell mutation.It accounts for about 50%of tumors in children and 80%of malignant tumors.[1]The nucleotide excisio...To the Editor:Glioma is a highly invasive and lethal heterogeneous disease caused by glial or precursor cell mutation.It accounts for about 50%of tumors in children and 80%of malignant tumors.[1]The nucleotide excision repair(NER)pathway is a process that involves the sequential assembly of many proteins,including excision repair cross complementation group 1(ERCC1)and xeroderma pigmentosum complementation group F(XPF,also known as ERCC4).展开更多
Variation of individuals’DNA repair capacity has been linked to cancer susceptibility.The xeroderma pigmentsum group F(XPF)plays a pivotal role in nucleotide-excision repair(NER)pathway.This study was to examine the ...Variation of individuals’DNA repair capacity has been linked to cancer susceptibility.The xeroderma pigmentsum group F(XPF)plays a pivotal role in nucleotide-excision repair(NER)pathway.This study was to examine the functional significance of XPF promoter polymorphisms and their association with lung cancer risk.The function of XPF promoter polymorphisms was tested by a set of biochemical assays,and their effects on lung cancer risk were determined by a case-control analysis of 988 patients with lung cancer and 986 controls.The XPF–673T allele showed a significantly higher transcriptional activity as compared with the–673C allele.The–673TT genotype was associated with a decreased risk of lung cancer compared with the CC genotype(adjusted OR=0.62,95%CI=0.42–0.91;P=0.015)and this effect was more significant among males(adjusted OR=0.55,95%CI=0.35–0.86;P=0.009),elder subjects(adjusted OR=0.51,95%CI=0.30–0.86;P=0.012),and light smokers(adjusted OR=0.35,95%CI=0.14–0.88;P=0.026).Thesefindings suggest that functional polymorphisms influencing DNA repair capa-city may confer susceptibility to lung cancer.展开更多
A new nitrogen-containing epoxy resin (XT resin) was synthesized from chain extension of xylenephenolformaldehyde resin (XPF) and triglycidyl isocyanurate (TGIC) in the presence of base catalyst. FT-IR and H-NMR ana...A new nitrogen-containing epoxy resin (XT resin) was synthesized from chain extension of xylenephenolformaldehyde resin (XPF) and triglycidyl isocyanurate (TGIC) in the presence of base catalyst. FT-IR and H-NMR analysis confirmed the chemical structure of XT resin. It was 1 cured with dicyandiamide (DICY) and diaminodiphenyl sulfone (DDS). Dynamic mechanical analysis (DMA) results showed that the introduction of triazine ring provides epoxy polymer with good thermal stability. Furthermore, high char yields at 800℃ in thermogravimetric (TGA) analysis indicated that XT resin had potential flame retardance.展开更多
文摘目的探索DNA损伤修复蛋白XPF表达水平影响肾癌细胞顺铂耐药的作用与机制。方法 Western blot法检测不同肾癌细胞株的XPF蛋白水平;采用shRNA干扰技术,筛选稳定低表达XPF的细胞株;采用克隆形成实验及细胞凋亡检测实验,比较XPF低表达细胞株与XPF正常表达细胞株的顺铂敏感性;通过β-半乳糖苷酶染色法,检测细胞的衰老程度。结果在肾癌细胞786-0、ACHN和Caki-1中,ACHN细胞的XPF表达水平最高,其顺铂耐药程度也最高。shRNA干扰获得稳定低表达XPF的ACHN细胞,顺铂处理后,其克隆形成率低于对照(P<0.05),细胞凋亡率高于对照,衰老细胞比例也显著高于对照(P<0.01)。结论肾癌化疗不敏感可能与其高表达DNA损伤修复蛋白XPF有关,干预并下调肾癌ACHN细胞XPF表达,可增强顺铂的凋亡诱导效应并加速衰老,从而提高其顺铂敏感性。
基金supported by grants from the National Natural Science Foundation of China(No.81802346)the China Postdoctoral Science Foundation(Nos.2020T130132,2020M682668)
文摘To the Editor:Glioma is a highly invasive and lethal heterogeneous disease caused by glial or precursor cell mutation.It accounts for about 50%of tumors in children and 80%of malignant tumors.[1]The nucleotide excision repair(NER)pathway is a process that involves the sequential assembly of many proteins,including excision repair cross complementation group 1(ERCC1)and xeroderma pigmentosum complementation group F(XPF,also known as ERCC4).
基金supported by the State Key Basic Research Program(No.2004CB518701).
文摘Variation of individuals’DNA repair capacity has been linked to cancer susceptibility.The xeroderma pigmentsum group F(XPF)plays a pivotal role in nucleotide-excision repair(NER)pathway.This study was to examine the functional significance of XPF promoter polymorphisms and their association with lung cancer risk.The function of XPF promoter polymorphisms was tested by a set of biochemical assays,and their effects on lung cancer risk were determined by a case-control analysis of 988 patients with lung cancer and 986 controls.The XPF–673T allele showed a significantly higher transcriptional activity as compared with the–673C allele.The–673TT genotype was associated with a decreased risk of lung cancer compared with the CC genotype(adjusted OR=0.62,95%CI=0.42–0.91;P=0.015)and this effect was more significant among males(adjusted OR=0.55,95%CI=0.35–0.86;P=0.009),elder subjects(adjusted OR=0.51,95%CI=0.30–0.86;P=0.012),and light smokers(adjusted OR=0.35,95%CI=0.14–0.88;P=0.026).Thesefindings suggest that functional polymorphisms influencing DNA repair capa-city may confer susceptibility to lung cancer.
文摘A new nitrogen-containing epoxy resin (XT resin) was synthesized from chain extension of xylenephenolformaldehyde resin (XPF) and triglycidyl isocyanurate (TGIC) in the presence of base catalyst. FT-IR and H-NMR analysis confirmed the chemical structure of XT resin. It was 1 cured with dicyandiamide (DICY) and diaminodiphenyl sulfone (DDS). Dynamic mechanical analysis (DMA) results showed that the introduction of triazine ring provides epoxy polymer with good thermal stability. Furthermore, high char yields at 800℃ in thermogravimetric (TGA) analysis indicated that XT resin had potential flame retardance.
