Objective:To investigate the association of XRCC4 polymorphic variants at G-1394T(rs6869366) with colorectal cancer susceptibility.Methods:In this hospital-based case-control study,the association of XRCC4 polymorphis...Objective:To investigate the association of XRCC4 polymorphic variants at G-1394T(rs6869366) with colorectal cancer susceptibility.Methods:In this hospital-based case-control study,the association of XRCC4 polymorphism with colorectal cancer risk in Chinese population was investigated.In total,171 patients with colorectal cancer and 171 healthy individuals matched for age and gender were selected.The genomic DNAs of the patients and controls were extracted from peripheral blood and the 300 bp target DNA was amplified with Polymerase Chain Reaction.The products were then digested with restriction endonuclease HincII,followed by agarose electrophoresis to identify the genotype.Results:We found a significant difference in the frequency of the XRCC4 G-1394T genotype between the colorectal cancer and control groups in female(1/127 vs 8/122,P<0.05).Those with G/T at XRCC4 G-1394T showed a decreased risk of colorectal cancer susceptibility compared with those with T/T(OR 0.113,95%CI 0.014-0.932).However,in overall population or in male,there was no significant difference of the distribution between the colorectal cancer and control groups.Conclusion:Our findings suggested that the G allele of XRCC4 G-1394T were associated with decreased risk of colorectal cancer susceptibility in female.展开更多
目的探讨XRCC4(X-ray cross-complementing group 4)作为检测结直肠癌的新型肿瘤标志物的应用价值。方法收集术前行PETCT检查的结直肠癌患者的切除肿瘤组织21例,应用ELISA法检测肿瘤组织中XRCC4蛋白表达情况,并与术前PETCT的标准摄取值(...目的探讨XRCC4(X-ray cross-complementing group 4)作为检测结直肠癌的新型肿瘤标志物的应用价值。方法收集术前行PETCT检查的结直肠癌患者的切除肿瘤组织21例,应用ELISA法检测肿瘤组织中XRCC4蛋白表达情况,并与术前PETCT的标准摄取值(SUV)相比对,进行相关性分析。结果肿瘤组织内XRCC4蛋白高表达,且与PETCT中SUV值密切相关(P<0.01)。结论 XRCC4蛋白作为新一代肿瘤标志物可能参与肿瘤细胞的能量代谢;结合PETCT检查,XRCC4蛋白可为结直肠癌的诊断与治疗提供一种新的思路。展开更多
文摘Objective:To investigate the association of XRCC4 polymorphic variants at G-1394T(rs6869366) with colorectal cancer susceptibility.Methods:In this hospital-based case-control study,the association of XRCC4 polymorphism with colorectal cancer risk in Chinese population was investigated.In total,171 patients with colorectal cancer and 171 healthy individuals matched for age and gender were selected.The genomic DNAs of the patients and controls were extracted from peripheral blood and the 300 bp target DNA was amplified with Polymerase Chain Reaction.The products were then digested with restriction endonuclease HincII,followed by agarose electrophoresis to identify the genotype.Results:We found a significant difference in the frequency of the XRCC4 G-1394T genotype between the colorectal cancer and control groups in female(1/127 vs 8/122,P<0.05).Those with G/T at XRCC4 G-1394T showed a decreased risk of colorectal cancer susceptibility compared with those with T/T(OR 0.113,95%CI 0.014-0.932).However,in overall population or in male,there was no significant difference of the distribution between the colorectal cancer and control groups.Conclusion:Our findings suggested that the G allele of XRCC4 G-1394T were associated with decreased risk of colorectal cancer susceptibility in female.
文摘目的探讨XRCC4(X-ray cross-complementing group 4)作为检测结直肠癌的新型肿瘤标志物的应用价值。方法收集术前行PETCT检查的结直肠癌患者的切除肿瘤组织21例,应用ELISA法检测肿瘤组织中XRCC4蛋白表达情况,并与术前PETCT的标准摄取值(SUV)相比对,进行相关性分析。结果肿瘤组织内XRCC4蛋白高表达,且与PETCT中SUV值密切相关(P<0.01)。结论 XRCC4蛋白作为新一代肿瘤标志物可能参与肿瘤细胞的能量代谢;结合PETCT检查,XRCC4蛋白可为结直肠癌的诊断与治疗提供一种新的思路。
