目的 探讨着色性干皮病G组（xeroderma pigmentosum group G,XPG）基因在不同年龄段健康汉族人群中的表达情况,分析XPG m RNA和蛋白表达量与年龄之间的相关性,以期为法医学年龄推断提供新的分子生物学指标。方法 收集150名不同年龄段健...目的 探讨着色性干皮病G组（xeroderma pigmentosum group G,XPG）基因在不同年龄段健康汉族人群中的表达情况,分析XPG m RNA和蛋白表达量与年龄之间的相关性,以期为法医学年龄推断提供新的分子生物学指标。方法 收集150名不同年龄段健康汉族人的外周血样,采用TRIzol法提取外周血单个核细胞（peripheral blood mononuclear cell,PBMC）总RNA,通过实时荧光定量PCR检测XPG m RNA在PBMC的相对表达量,酶联免疫吸附试验检测XPG蛋白在血浆中的表达量。结果 XPG m RNA及其蛋白表达量在≤18岁组与19~45岁组之间、≤18岁组与≥46岁组之间的差异均有统计学意义（P〈0.05）,但19~45岁组与≥46岁组之间的差异无统计学意义（P〉0.05）。XPG m RNA和蛋白表达量均无性别差异（P〉0.05）。结论 XPG m RNA在PBMC的相对表达量在低龄段内随年龄增加而下降,其血浆中蛋白随年龄增加而升高;XPG基因有望成为法医学年龄推断的新型指标之一。展开更多
BACKGROUND The xeroderma pigmentosum group G(XPG)gene at chromosome 13q33 consists of 15 exons,which may be related to the occurrence and development of gastric cancer(GC).AIM To examine the association of several com...BACKGROUND The xeroderma pigmentosum group G(XPG)gene at chromosome 13q33 consists of 15 exons,which may be related to the occurrence and development of gastric cancer(GC).AIM To examine the association of several common single nucleotide polymorphisms(SNPs)of the XPG gene with GC risk and survival.METHODS Five SNPs of XPG(rs2094258,rs751402,rs873601,rs2296147,and rs1047768)were genotyped by PCR restriction fragment length polymorphism in 956 histologically confirmed GC cases and 1012 controls in North China.GC patients were followed for survival status and,if deceased,cause of death.Logistic regression and Cox regression were used for analysing associations of XPG SNPs with risk of GC and prognosis,respectively.For rs2094258,heterozygous model(CT vs CC),homozygous model(TT vs CC),recessive model(TT vs CT+CC),and dominant model(TT+CT vs CC)were analyzed.RESULTS None of the examined loci were statistically associated with GC risk,although rs2296147 was marginally associated with GC risk(P=0.050).GC patients with the rs2094258 CT+CC genotype showed worse survival than those with the TT genotype(log-rank test,P=0.028),and patients with the CC genotype had a tendency of unfavourable prognosis compared with those with the TT+CT genotype(log-rank test,P=0.039).The increase in C alleles of rs2094258[hazard ratio(HR)=1.19,95%confidence interval(CI):1.02-1.45,P=0.037]were associated with the long-term survival of GC cases.Other risk factors for survival included tumor differentiation(HR=4.51,95%CI:1.99-8.23,P<0.001),lymphovascular invasion(HR=1.97,95%CI:1.44-3.01,P<0.001),and use of chemotherapy(HR=0.81,95%CI:0.63-0.98,P=0.041).CONCLUSION The XPG rs2094258 polymorphism may be associated with overall survival in GC patients.展开更多
目的探讨苯并芘(Benzo[a]pyrene,BaP)作用下的人支气管上皮细胞(16HBE)热休克蛋白70(Heatshock protein 70,Hsp70)和着色性干皮病G蛋白(Xeroderma pigmentosum group G,XPG)表达的时间效应特征,并分析二者之间可能存在的关联性。方法用8...目的探讨苯并芘(Benzo[a]pyrene,BaP)作用下的人支气管上皮细胞(16HBE)热休克蛋白70(Heatshock protein 70,Hsp70)和着色性干皮病G蛋白(Xeroderma pigmentosum group G,XPG)表达的时间效应特征,并分析二者之间可能存在的关联性。方法用8μmol/L BaP处理16HBE细胞0、1、2、4、8、12、24和48 h,以彗星实验检测细胞DNA损伤并以Olive尾矩值(Olive Tail Moments,OTM)评价DNA损伤程度,以Western-blot检测Hsp70和XPG的表达水平,并以激光共聚焦法检测二者的共定位。结果染毒1～2h时OTM值变化率最大,与正常细胞相比,除染毒1h组外其余各组OTM值均显著性增高(P﹤0.01);Hsp70和XPG的表达随染毒时间逐渐增高,12 h时达到峰值,激光共聚焦结果显示BaP染毒细胞后Hsp70和XPG的结合在细胞核内增强。结论在该试验条件下,XPG在执行核苷酸切除修复作用时可能有Hsp70的参与,其具体机制还有待其他实验进一步加以证实。展开更多
文摘目的 探讨着色性干皮病G组（xeroderma pigmentosum group G,XPG）基因在不同年龄段健康汉族人群中的表达情况,分析XPG m RNA和蛋白表达量与年龄之间的相关性,以期为法医学年龄推断提供新的分子生物学指标。方法 收集150名不同年龄段健康汉族人的外周血样,采用TRIzol法提取外周血单个核细胞（peripheral blood mononuclear cell,PBMC）总RNA,通过实时荧光定量PCR检测XPG m RNA在PBMC的相对表达量,酶联免疫吸附试验检测XPG蛋白在血浆中的表达量。结果 XPG m RNA及其蛋白表达量在≤18岁组与19~45岁组之间、≤18岁组与≥46岁组之间的差异均有统计学意义（P〈0.05）,但19~45岁组与≥46岁组之间的差异无统计学意义（P〉0.05）。XPG m RNA和蛋白表达量均无性别差异（P〉0.05）。结论 XPG m RNA在PBMC的相对表达量在低龄段内随年龄增加而下降,其血浆中蛋白随年龄增加而升高;XPG基因有望成为法医学年龄推断的新型指标之一。
基金Supported by the Fundamental Research Funds for the Central Universities,No.zdyf2017007
文摘BACKGROUND The xeroderma pigmentosum group G(XPG)gene at chromosome 13q33 consists of 15 exons,which may be related to the occurrence and development of gastric cancer(GC).AIM To examine the association of several common single nucleotide polymorphisms(SNPs)of the XPG gene with GC risk and survival.METHODS Five SNPs of XPG(rs2094258,rs751402,rs873601,rs2296147,and rs1047768)were genotyped by PCR restriction fragment length polymorphism in 956 histologically confirmed GC cases and 1012 controls in North China.GC patients were followed for survival status and,if deceased,cause of death.Logistic regression and Cox regression were used for analysing associations of XPG SNPs with risk of GC and prognosis,respectively.For rs2094258,heterozygous model(CT vs CC),homozygous model(TT vs CC),recessive model(TT vs CT+CC),and dominant model(TT+CT vs CC)were analyzed.RESULTS None of the examined loci were statistically associated with GC risk,although rs2296147 was marginally associated with GC risk(P=0.050).GC patients with the rs2094258 CT+CC genotype showed worse survival than those with the TT genotype(log-rank test,P=0.028),and patients with the CC genotype had a tendency of unfavourable prognosis compared with those with the TT+CT genotype(log-rank test,P=0.039).The increase in C alleles of rs2094258[hazard ratio(HR)=1.19,95%confidence interval(CI):1.02-1.45,P=0.037]were associated with the long-term survival of GC cases.Other risk factors for survival included tumor differentiation(HR=4.51,95%CI:1.99-8.23,P<0.001),lymphovascular invasion(HR=1.97,95%CI:1.44-3.01,P<0.001),and use of chemotherapy(HR=0.81,95%CI:0.63-0.98,P=0.041).CONCLUSION The XPG rs2094258 polymorphism may be associated with overall survival in GC patients.
