In this study, a simple, reliable and accurate method for the simultaneous separation and determination of naringin, hesperidin, neohesperidin, baicalin, wogonoside, baicalein, wogonin, emodin and chrysophanol in ‘Da...In this study, a simple, reliable and accurate method for the simultaneous separation and determination of naringin, hesperidin, neohesperidin, baicalin, wogonoside, baicalein, wogonin, emodin and chrysophanol in ‘Da-Chai-Hu-Tang’ was developed by reverse-phase high-performance liquid chromatography (RP-HPLC). The chromatographic separation was performed on an Agilent ZORBAX C18 column (250 mm × 4.6 mm i.d., 5.0 μm), and the mobile phase composed of methanol and water containing 1% (v/v) acetic acid was used to elute the targets in a gradient elution mode. The flow rate and detection wavelength were set at 0.8 ml/min and 280 nm, respectively. All calibration curves of the nine components expressed good linearities (r2≥0.9992) within the tested ranges. The RSD values demonstrated the intra- and inter-day precisions were less than 2.89%, and the recoveries of the investigated compounds were between 96.22% and 105.28%. The proposed method is simple, precise, specific, sensitive, and successfully applied to determine the nine marker compounds in ‘Da-Chai-Hu-Tang’ for quality control.展开更多
An HPLC-DAD-ELSD method was developed and validated for the simultaneous quantitation of seven active components（liquiritin, baicalin, wogonoside, baicalein, wogonin, ginsenosides Re and ginsenosides Rb1） in n-butan...An HPLC-DAD-ELSD method was developed and validated for the simultaneous quantitation of seven active components（liquiritin, baicalin, wogonoside, baicalein, wogonin, ginsenosides Re and ginsenosides Rb1） in n-butanol effective fraction of Xiao Chai Hu Tang. A Diamonsil C18（2） column（4.6 mm × 250 mm, 5 μm） was used as the stationary phase and the mobile phase was consisted of acetonitrile and aqueous phosphate acid（0.05%, v/v）. Gradient elution was carried out at the flow rate of 1 m L/min. The detection wavelength was set at 276 nm and an evaporative light scattering detector was also used. Good linearity for all the seven active components was observed. The established method is simple, fast, reliable, and suitable for the quality control of n-butanol effective fraction of Xiao Chai Hu Tang.展开更多
The objective of this study is to summarize the pharmacological effects and the mechanisms of action of Xiao Chai Hu Tang(XCHT, Minor Bupleurum Decoction) on liver diseases, so as to give relevant researchers a valuab...The objective of this study is to summarize the pharmacological effects and the mechanisms of action of Xiao Chai Hu Tang(XCHT, Minor Bupleurum Decoction) on liver diseases, so as to give relevant researchers a valuable insight and benefit patients with hepatopathy. PubMed was used to search for and collect scientific publications related to XCHT and liver diseases from 1986 to 2016. The available scientific results or evidence were read, classified, and analyzed. XCHT showed clinical efficacy in patients with hepatic diseases including hepatitis,hepatic fibrosis, and hepatoma. The mechanisms involved the production of cytokines, the regulation of immune function, the suppression of lipid peroxidation, etc., XCHT might work on the metabolism of some medications such as tolbutamide by the regulation of gastric emptying and intragastric pH. XCHT exhibited a very low toxicity profile, such as interstitial pneumonia due to duration of medication,patients' age, and drug combination. XCHT has been a eutherapeutic supplemental remedy for liver diseases. However, many mechanisms of action and effects of XCHT on new types of liver diseases still remain unclear, so more and more animal experiments and human clinical trials are needed to obtain enough proofs for the clinical use of XCHT in new types of hepatosis such as nonalcoholic fatty liver disease and autoimmune liver disease.展开更多
文摘In this study, a simple, reliable and accurate method for the simultaneous separation and determination of naringin, hesperidin, neohesperidin, baicalin, wogonoside, baicalein, wogonin, emodin and chrysophanol in ‘Da-Chai-Hu-Tang’ was developed by reverse-phase high-performance liquid chromatography (RP-HPLC). The chromatographic separation was performed on an Agilent ZORBAX C18 column (250 mm × 4.6 mm i.d., 5.0 μm), and the mobile phase composed of methanol and water containing 1% (v/v) acetic acid was used to elute the targets in a gradient elution mode. The flow rate and detection wavelength were set at 0.8 ml/min and 280 nm, respectively. All calibration curves of the nine components expressed good linearities (r2≥0.9992) within the tested ranges. The RSD values demonstrated the intra- and inter-day precisions were less than 2.89%, and the recoveries of the investigated compounds were between 96.22% and 105.28%. The proposed method is simple, precise, specific, sensitive, and successfully applied to determine the nine marker compounds in ‘Da-Chai-Hu-Tang’ for quality control.
基金Foundation for Young Scholars of Shanxi Province,China(Grant No.2011021007-2)
文摘An HPLC-DAD-ELSD method was developed and validated for the simultaneous quantitation of seven active components（liquiritin, baicalin, wogonoside, baicalein, wogonin, ginsenosides Re and ginsenosides Rb1） in n-butanol effective fraction of Xiao Chai Hu Tang. A Diamonsil C18（2） column（4.6 mm × 250 mm, 5 μm） was used as the stationary phase and the mobile phase was consisted of acetonitrile and aqueous phosphate acid（0.05%, v/v）. Gradient elution was carried out at the flow rate of 1 m L/min. The detection wavelength was set at 276 nm and an evaporative light scattering detector was also used. Good linearity for all the seven active components was observed. The established method is simple, fast, reliable, and suitable for the quality control of n-butanol effective fraction of Xiao Chai Hu Tang.
基金supported by grants from the Science and Technology Commission Foundation of Shanghai MunicipalityChina(grants 13401902801)
文摘The objective of this study is to summarize the pharmacological effects and the mechanisms of action of Xiao Chai Hu Tang(XCHT, Minor Bupleurum Decoction) on liver diseases, so as to give relevant researchers a valuable insight and benefit patients with hepatopathy. PubMed was used to search for and collect scientific publications related to XCHT and liver diseases from 1986 to 2016. The available scientific results or evidence were read, classified, and analyzed. XCHT showed clinical efficacy in patients with hepatic diseases including hepatitis,hepatic fibrosis, and hepatoma. The mechanisms involved the production of cytokines, the regulation of immune function, the suppression of lipid peroxidation, etc., XCHT might work on the metabolism of some medications such as tolbutamide by the regulation of gastric emptying and intragastric pH. XCHT exhibited a very low toxicity profile, such as interstitial pneumonia due to duration of medication,patients' age, and drug combination. XCHT has been a eutherapeutic supplemental remedy for liver diseases. However, many mechanisms of action and effects of XCHT on new types of liver diseases still remain unclear, so more and more animal experiments and human clinical trials are needed to obtain enough proofs for the clinical use of XCHT in new types of hepatosis such as nonalcoholic fatty liver disease and autoimmune liver disease.
