Xiao-Ai-Ping(XAP),a traditional oriental medicinal herb isolated from the stem of Marsdenia tenacissima(Roxb.)Wight et Arn.,has been shown to induce tumor cell apoptosis.In this study,we used confocal fluorescence mic...Xiao-Ai-Ping(XAP),a traditional oriental medicinal herb isolated from the stem of Marsdenia tenacissima(Roxb.)Wight et Arn.,has been shown to induce tumor cell apoptosis.In this study,we used confocal fluorescence microscopy and fluorescence resonance energy transfer(FRET)techniques to study the molecular mechanism of XAPinduced apoptosis in single living human lung adenocarcinoma(ASTC-a-1)cells.The efficacious apoptosis was observed at 6 h after of 100μl XAP treatment.Further monitoring the dynamics of caspase-3 activation using FRET imaging in single living ASTC-a-1 cell expressing stably with SCAT3,a FRET probe,showed that XAP activated the caspase-3 at about 2 h after XAP treatment.These data suggest that caspase-3 activation was involved in the XAP-induced apoptosis in ASTC-a-1 cells.展开更多
消癌平注射液(XAP-Ⅰ)提取自通关藤,在我国临床上广泛用于治疗各种癌症,而从其复杂组分中阐释生物活性成分一直是学界研究的热点内容。本文结合三重四级杆质谱仪中的全扫描(full scan,FS)、母离子扫描(precursor ion scan,PCIS)、子离...消癌平注射液(XAP-Ⅰ)提取自通关藤,在我国临床上广泛用于治疗各种癌症,而从其复杂组分中阐释生物活性成分一直是学界研究的热点内容。本文结合三重四级杆质谱仪中的全扫描(full scan,FS)、母离子扫描(precursor ion scan,PCIS)、子离子扫描(product ion,PDIS)及多反应监测(multiple reaction monitoring,MRM)模式鉴定了XAP-Ⅰ中的主要孕甾烷糖苷及酚酸类成分,可利用丰富的加合离子信息确定组分结构特性并实现高灵敏度分析。此外,本文应用定向细胞结合植物化学组分筛选策略以发现XAP-Ⅰ中的生物活性成分,该策略可实现对XAP-Ⅰ的高通量定性、定量分析,并显著提高XAP-Ⅰ潜在生物活性成分筛选的灵敏度及选择性。展开更多
基金supported by National Natural Science Foundation of China(Grant No.30670507)the Opening Project of MOE Key laboratory of Laser Life Science,South China Normal University,Guangzhou 510631,China.
文摘Xiao-Ai-Ping(XAP),a traditional oriental medicinal herb isolated from the stem of Marsdenia tenacissima(Roxb.)Wight et Arn.,has been shown to induce tumor cell apoptosis.In this study,we used confocal fluorescence microscopy and fluorescence resonance energy transfer(FRET)techniques to study the molecular mechanism of XAPinduced apoptosis in single living human lung adenocarcinoma(ASTC-a-1)cells.The efficacious apoptosis was observed at 6 h after of 100μl XAP treatment.Further monitoring the dynamics of caspase-3 activation using FRET imaging in single living ASTC-a-1 cell expressing stably with SCAT3,a FRET probe,showed that XAP activated the caspase-3 at about 2 h after XAP treatment.These data suggest that caspase-3 activation was involved in the XAP-induced apoptosis in ASTC-a-1 cells.
基金The Natural Science Foundation of Gansu Province (Grant No. 21JR7RA353)the First Hospital of Lanzhou University Science Foundations (Grant No. ldyyyn2018-08)the Research Foundation of Education Bureau of Gansu Province,China (Grant No. 2020B-001)。
文摘消癌平注射液(XAP-Ⅰ)提取自通关藤,在我国临床上广泛用于治疗各种癌症,而从其复杂组分中阐释生物活性成分一直是学界研究的热点内容。本文结合三重四级杆质谱仪中的全扫描(full scan,FS)、母离子扫描(precursor ion scan,PCIS)、子离子扫描(product ion,PDIS)及多反应监测(multiple reaction monitoring,MRM)模式鉴定了XAP-Ⅰ中的主要孕甾烷糖苷及酚酸类成分,可利用丰富的加合离子信息确定组分结构特性并实现高灵敏度分析。此外,本文应用定向细胞结合植物化学组分筛选策略以发现XAP-Ⅰ中的生物活性成分,该策略可实现对XAP-Ⅰ的高通量定性、定量分析,并显著提高XAP-Ⅰ潜在生物活性成分筛选的灵敏度及选择性。
