XiaoShuanTongLuo （XSTL） is an important multi-herbal formula widely used as a traditional Chinese medicine （TCM）. In this paper, an ultra-performance liquid chromatography-electrospray ionization tandem mass spect...XiaoShuanTongLuo （XSTL） is an important multi-herbal formula widely used as a traditional Chinese medicine （TCM）. In this paper, an ultra-performance liquid chromatography-electrospray ionization tandem mass spectrometric method （UPLC-ESI-MS/MS） was developed for the simultaneous identification and quantification of 18 chemical constituents in the extract of XSTL. The analysis was performed in multiple-reaction monitoring （MRM） mode using positive and negative electrospray ionization （ESI）. A BEH C18 （2.1 mm× 100 mm, 1.7 μm） column with a gradient mobile phase of methanol-0.1% aqueous formic acid solution at a flow rate of 0.2 mL/min was used for separation. The standard curves of most components showed good linearity over the concentration range of 0.001-0.5 μg/mL with r = 0.9896-0.9996. The relative standard deviations （RSD） of intra-day and inter-day precision were all lower than 15% at low, middle, and high concentration levels. The RSD of stability analysis in 24 h were also lower than 15%. The validated method was employed to quantify 18 active components simultaneously in four different commercial brands of XSTL products in various dosage forms. The developed method was rapid, accurate, sensitive, and reproducible for the quality control of XSTL.展开更多
在"中药复方有效成分组"理论指导下,观察消栓通络方有效成分组(the effective components group of Xiaoshuantongluo formula,XECG)对大鼠急性血瘀模型的影响。采用皮下注射肾上腺素加冰水浴制备大鼠急性血瘀模型,评价XECG...在"中药复方有效成分组"理论指导下,观察消栓通络方有效成分组(the effective components group of Xiaoshuantongluo formula,XECG)对大鼠急性血瘀模型的影响。采用皮下注射肾上腺素加冰水浴制备大鼠急性血瘀模型,评价XECG对全血黏度、血浆黏度、红细胞聚集率、血小板聚集率等血液流变学指标,PT、APTT、TT和FIB等凝血指标以及体内6-keto-PGF1α、TXB2和D-dimer水平的影响。结果表明,XECG显著降低ADP诱导的血小板聚集,但对全血黏度、血浆黏度、红细胞聚集率无明显影响;能延长PT和TT,对APTT和FIB含量无影响;可显著降低急性血瘀大鼠血浆中D-dimer水平,轻微降低TXB2的含量,但对6-keto-PGF1α的含量无影响。结果提示,XECG的作用在抗血小板聚集方面表现得更为突出和专一。展开更多
基金National Natural Science Foundation of China(Grant No.30630073)
文摘XiaoShuanTongLuo （XSTL） is an important multi-herbal formula widely used as a traditional Chinese medicine （TCM）. In this paper, an ultra-performance liquid chromatography-electrospray ionization tandem mass spectrometric method （UPLC-ESI-MS/MS） was developed for the simultaneous identification and quantification of 18 chemical constituents in the extract of XSTL. The analysis was performed in multiple-reaction monitoring （MRM） mode using positive and negative electrospray ionization （ESI）. A BEH C18 （2.1 mm× 100 mm, 1.7 μm） column with a gradient mobile phase of methanol-0.1% aqueous formic acid solution at a flow rate of 0.2 mL/min was used for separation. The standard curves of most components showed good linearity over the concentration range of 0.001-0.5 μg/mL with r = 0.9896-0.9996. The relative standard deviations （RSD） of intra-day and inter-day precision were all lower than 15% at low, middle, and high concentration levels. The RSD of stability analysis in 24 h were also lower than 15%. The validated method was employed to quantify 18 active components simultaneously in four different commercial brands of XSTL products in various dosage forms. The developed method was rapid, accurate, sensitive, and reproducible for the quality control of XSTL.
文摘在"中药复方有效成分组"理论指导下,观察消栓通络方有效成分组(the effective components group of Xiaoshuantongluo formula,XECG)对大鼠急性血瘀模型的影响。采用皮下注射肾上腺素加冰水浴制备大鼠急性血瘀模型,评价XECG对全血黏度、血浆黏度、红细胞聚集率、血小板聚集率等血液流变学指标,PT、APTT、TT和FIB等凝血指标以及体内6-keto-PGF1α、TXB2和D-dimer水平的影响。结果表明,XECG显著降低ADP诱导的血小板聚集,但对全血黏度、血浆黏度、红细胞聚集率无明显影响;能延长PT和TT,对APTT和FIB含量无影响;可显著降低急性血瘀大鼠血浆中D-dimer水平,轻微降低TXB2的含量,但对6-keto-PGF1α的含量无影响。结果提示,XECG的作用在抗血小板聚集方面表现得更为突出和专一。
