Background:Fracture is a common large-organ traumatic injury and usually leads to complications in other systems.Multiple fractures are often associated with gastrointestinal(GI)dysfunction.However,no study has evalua...Background:Fracture is a common large-organ traumatic injury and usually leads to complications in other systems.Multiple fractures are often associated with gastrointestinal(GI)dysfunction.However,no study has evaluated the treatment of GI dysfunction.The purpose of the study is to explore the efficacy and mechanism based on ICC pathway of Banxia-Houpu decoction on GI.Methods:Male Sprague-Dawley mice were randomly divided according to the fracture modeling method and intervention.We detected the“GI residual rate”and motility.Gastric antrum and jejunum tissues were dissected for hematoxylin and eosin staining to observe the structural integrity of the GI tract,and immunohistochemistry was used to detect the expression of c-kit protein.Results:Compared with the fracture group the GI residual rates in the Banxia Houpu decoction and mosapride groups were significantly low,while GI motility was significantly high.HE staining revealed significant GI tissue necrosis and inflammatory cell infiltrations in the fracture groups.The Banxia Houpu decoction and mosapride groups,exhibited less pathology.Immunohistochemical staining showed upregulated c-kit protein expression in the fracture groups.The c-kit protein level was decreased in the mosapride group.Additionally,c-kit protein expression in the Banxia Houpu decoction groups was significantly decreased in a concentration-dependent manner.Conclusion:Banxia Houpu decoction improves GI dysfunction after multiple fractures,reduces inflammation and necrosis of gastric epithelial cells,and inhibits c-kit protein expression in GI tissues of mice.Results showed revealed one of the mechanisms underlying the effects of this decoction on the GI dysfunction in mice after multiple fractures.展开更多
Gallbladder cancer(GBC)is a malignancy of biliary tract which is infrequent in developed countries but common in some specific geographical regions of developingCurrently,GBC has a low early diagnosis rate and an extr...Gallbladder cancer(GBC)is a malignancy of biliary tract which is infrequent in developed countries but common in some specific geographical regions of developingCurrently,GBC has a low early diagnosis rate and an extremely poor prognosis,leading to major problems for treatment of GBC.Liver invasion and metastasis one of the main causes of its poor prognosis,with its average overall survival of 6 months.展开更多
基金supported by Natural Science Foundation of Zhejiang Province(grant number LY20H270012)Zhejiang Traditional Chinese Medicine Administration(2020ZB090)+1 种基金Zhejiang Chinese Medical University Research Fund(2021JKGJYY011)Hua Jiang Famous Expert Inheritance Studio of Traditional Chinese Medicine(GZS2021020).
文摘Background:Fracture is a common large-organ traumatic injury and usually leads to complications in other systems.Multiple fractures are often associated with gastrointestinal(GI)dysfunction.However,no study has evaluated the treatment of GI dysfunction.The purpose of the study is to explore the efficacy and mechanism based on ICC pathway of Banxia-Houpu decoction on GI.Methods:Male Sprague-Dawley mice were randomly divided according to the fracture modeling method and intervention.We detected the“GI residual rate”and motility.Gastric antrum and jejunum tissues were dissected for hematoxylin and eosin staining to observe the structural integrity of the GI tract,and immunohistochemistry was used to detect the expression of c-kit protein.Results:Compared with the fracture group the GI residual rates in the Banxia Houpu decoction and mosapride groups were significantly low,while GI motility was significantly high.HE staining revealed significant GI tissue necrosis and inflammatory cell infiltrations in the fracture groups.The Banxia Houpu decoction and mosapride groups,exhibited less pathology.Immunohistochemical staining showed upregulated c-kit protein expression in the fracture groups.The c-kit protein level was decreased in the mosapride group.Additionally,c-kit protein expression in the Banxia Houpu decoction groups was significantly decreased in a concentration-dependent manner.Conclusion:Banxia Houpu decoction improves GI dysfunction after multiple fractures,reduces inflammation and necrosis of gastric epithelial cells,and inhibits c-kit protein expression in GI tissues of mice.Results showed revealed one of the mechanisms underlying the effects of this decoction on the GI dysfunction in mice after multiple fractures.
文摘Gallbladder cancer(GBC)is a malignancy of biliary tract which is infrequent in developed countries but common in some specific geographical regions of developingCurrently,GBC has a low early diagnosis rate and an extremely poor prognosis,leading to major problems for treatment of GBC.Liver invasion and metastasis one of the main causes of its poor prognosis,with its average overall survival of 6 months.
文摘目的探讨咳喘定穴位贴敷联合小柴胡汤合半夏厚朴汤治疗痰浊阻肺证慢性阻塞性肺疾病(Chronic obstructive pulmonary disease,COPD)患者的临床疗效。方法选取2020年9月—2022年10月期间海南医学院第二附属医院收治的痰浊阻肺证COPD患者144例,按随机数字表法分为西药组、中药组、中药联合贴敷组,每组各48例。西药组采取常规西医治疗,中药组在西药基础上采取小柴胡汤合半夏厚朴汤治疗,中药联合贴敷组在常规西医基础上采取咳喘定穴位贴敷及小柴胡汤合半夏厚朴汤。治疗4周后,观察比较3组患者临床疗效、不良反应发生率、疾病复发率及治疗前后中医证候积分、肺功能[第1秒用力呼气容积(Forced expiratory volume for 1 second,FEV_(1))、用力肺活量(Forced vital capacity,FVC)、FEV_(1)/FVC]、康复效果[慢性阻塞性肺疾病评估测试量表(Chronic obstructive Pulmonary Disease Assessment Test Scale,CAT)、呼吸困难程度评分(modified Medical Research Council,mMRC)、6 min步行距离试验(6 min walking distance test,6MWT)]。结果治疗后中药联合贴敷组总有效率93.75%(45/48)高于西药组77.08%(37/48)、中药组79.17%(38/48),差异有统计学意义(P<0.05)。治疗后3组患者中医证候痰多、喘息、咳嗽、倦怠乏力、腹胀纳呆、下肢水肿积分均较治疗前降低,差异有统计学意义(P<0.05),且中药联合贴敷组中医证候痰多、喘息、咳嗽、倦怠乏力、腹胀纳呆、下肢水肿积分均明显低于西药组、中药组,差异有统计学意义(P<0.05)。治疗后3组患者肺功能FEV_(1)、FVC、FEV_(1)/FVC指标均较治疗前升高,差异有统计学意义(P<0.05);且中药联合贴敷组肺功能FEV_(1)、FVC、FEV_(1)/FVC指标明显高于西药组、中药组,差异有统计学意义(P<0.05)。治疗后3组患者康复效果CAT、mMRC评分较治疗前降低,6MWT较治疗前延长,差异有统计学意义(P<0.05);且中药联合贴敷组康复效果CAT、mMRC评分均明显低于西药组、中药组,6MWT明显优于西药组、中药组,差异有统计学意义(P<0.05)。治疗期间,中药联合贴敷组不良反应发生率8.33%(4/48)与西药组6.25%(3/48)、中药组4.17%(2/48)比较,差异无统计学意义(P>0.05)。治疗6个月后中药联合贴敷组疾病复发率4.17%(2/48)低于西药组18.75%(9/48)、中药组16.67(8/48),差异有统计学意义(P<0.05)。结论联合采取咳喘定穴位贴敷及小柴胡汤合半夏厚朴汤治疗痰浊阻肺证COPD效果显著,可有效缓解患者临床症状,改善肺功能及机体运动耐力,利于提升疾病整体干预效果,降低疾病复发率,且具有安全性。
