目的探究中药复方消银颗粒对咪喹莫特诱导的银屑病小鼠的改善作用及其潜在的信号通路。方法采用咪喹莫特乳膏涂抹构建银屑病小鼠模型,随机分为以下5组:对照组(control)、咪喹莫特(IMQ)组、中药复方消银颗粒低剂量组、中药复方消银颗粒...目的探究中药复方消银颗粒对咪喹莫特诱导的银屑病小鼠的改善作用及其潜在的信号通路。方法采用咪喹莫特乳膏涂抹构建银屑病小鼠模型,随机分为以下5组:对照组(control)、咪喹莫特(IMQ)组、中药复方消银颗粒低剂量组、中药复方消银颗粒高剂量组、阿维A组。观察不同组小鼠皮损严重程度,分析各组小鼠皮损面积和严重程度指数(psoriasis area and severity index,PASI)评分,处死小鼠后收集血清,HE染色观察各组小鼠组织形态。CCK-8检测含药血清处理后HaCaT细胞增殖活力,流式细胞仪检测不同处理组HaCaT细胞凋亡情况,Western blot检测HaCaT细胞内JAK/STAT3通路的激活情况。结果与对照组相比,模型组小鼠出现了明显的银屑病病变,红斑、鳞屑明显增加,表皮厚度增加,而中药复方消银颗粒与阿维A作用组小鼠PASI评分明显降低,症状明显减轻。不同浓度含药血清(2.5%、5%和10%)作用HaCaT细胞后,可抑制其增殖并促进凋亡。此外5%、10%含药血清作用细胞后,能显著抑制JAK、p-JAK、STAT3、p-STAT3蛋白水平。结论中药复方消银颗粒通过抑制JAK/STAT3通路激活在缓解银屑病中发挥重要效应。展开更多
Objective： To explore the regulatory mechanism of Xiaoyin Recipe （消银方） on the T helper 1/ T helper 2 （Th1/Th2） immune balance. Methods： Thirty-six experimental animals were divided into three groups, 12 rats ...Objective： To explore the regulatory mechanism of Xiaoyin Recipe （消银方） on the T helper 1/ T helper 2 （Th1/Th2） immune balance. Methods： Thirty-six experimental animals were divided into three groups, 12 rats in each group： blank control group （B group）, negative control group （N group）, and Xiaoyin Recipe treatment group （T group）. The latter two groups received immunization of experimental autoimmune thyroiditis （EAT）, and T group were treated with Xiaoyin Recipe for a month. Then, the expression of Th1-Th2-related genes in peripheral blood mononuclear cells （PBMCs） were screened with Oligo GEArray Rat Thl-Th2-Th3 Microarray. The expressions of tumor necrosis factor- α （TNF- α）, interleukin-10 （IL-10）, T-box expressed in T-cells （T-bet）, and GATA-binding protein-3 （GATA-3） were detected by real-time polymerase chain reaction （RT-PCR）. Results： Gene array screening showed that compared to N group, in T group after Xiaoyin Recipe treatment, 3 genes were upregulated in EAT rats, including interleukin-27 receptor alpha （IL-27roL）, glomulin （GImn）, and GATA-3, while 38 genes were downregulated, such as CD28, IL-18, signal transducer, and activator of transcription 1 （STAT1）, T-bet, TNF receptor superfamily member 4 （TNFRSF4）, TNF ligand superfamily member 5 （TNFSF5）, and TNF receptor superfamily member 5 （TNFRSF5）. While RT-PCR showed that there was an increased level of TNF-α mRNA （P〈0.01）, an elevated ratio of T-bet/GATA-3, and a decreased level of IL-10 mRNA in PBMC of N and T group compared to B group （P〈0.01）; and after treatment with Xiaoyin Recipe, IL-10 mRNA level increased （P〈0.01）, while TNF-α mRNA level and T-bet/GATA-3 ratio decreased in T group compared to N group （P〈0.01）. Conclusion： Xiaoyin Recipe for psoriasis could induce a Thl/Th2 balance drift toward Th2 in PBMC of EAT rats and thus improve the conditions.展开更多
OBJECTIVE:To evaluate the effectiveness of Xiaoyin Jiedu(XYJD)granules in the treatment of psoriasis vulgaris(PSV)in patients with a blood-heat pattern(BHP)in terms of Traditional Chinese Medicine(TCM).We also aimed t...OBJECTIVE:To evaluate the effectiveness of Xiaoyin Jiedu(XYJD)granules in the treatment of psoriasis vulgaris(PSV)in patients with a blood-heat pattern(BHP)in terms of Traditional Chinese Medicine(TCM).We also aimed to identify the possible underlying immunological mechanism.METHODS:Twenty-five PSV patients with BHP and ten normal controls were enrolled from January 1,2015 to December 31,2016.Patients were randomly assigned to either the XYJD group(15 cases)or the placebo group(10 cases),in which patients were treated with XYJD granules or a placebo,respectively.Additionally,albolene was used to relieve skin dryness in these two groups.The psoriasis area and severity indexes,dermatology life quality indexes and itching scores were assessed at the end of the 2 nd,4 th and 8 th week of treatment.The number of peripheral blood T helper(Th)9,Th17 and regulatory T cells(Tregs)and the m RNA and protein expression levels of PU.1,RAR-related orphan receptor(ROR)-γt,forkhead box protein 3(Foxp3),interleukin(IL)-9,IL-17,IL-23 and IL-10 in the control and experimental groups were compared before and after treatment.RESULTS:Psoriasis area and severity indexes and itching scores of patients in the XYJD group were significantly lower than those in the placebo group,whereas dermatology life quality indexes were significantly higher.In comparison with the placebo group,XYJD granules significantly reduced the number of Th17 cells and the m RNA and protein expression levels of Th17-related ROR-γt,IL-17,IL-22 and IL-23 in the peripheral blood and reduced the number of Th9 cells and the m RNA and protein expression levels of Th9-related PU.1 and IL-9.CONCLUSION:XYJD granules were effective against PSV in patients with BHP by reducing the number of Th9 and Th17 cells and the levels of their related cytokines.展开更多
文摘目的探究中药复方消银颗粒对咪喹莫特诱导的银屑病小鼠的改善作用及其潜在的信号通路。方法采用咪喹莫特乳膏涂抹构建银屑病小鼠模型,随机分为以下5组:对照组(control)、咪喹莫特(IMQ)组、中药复方消银颗粒低剂量组、中药复方消银颗粒高剂量组、阿维A组。观察不同组小鼠皮损严重程度,分析各组小鼠皮损面积和严重程度指数(psoriasis area and severity index,PASI)评分,处死小鼠后收集血清,HE染色观察各组小鼠组织形态。CCK-8检测含药血清处理后HaCaT细胞增殖活力,流式细胞仪检测不同处理组HaCaT细胞凋亡情况,Western blot检测HaCaT细胞内JAK/STAT3通路的激活情况。结果与对照组相比,模型组小鼠出现了明显的银屑病病变,红斑、鳞屑明显增加,表皮厚度增加,而中药复方消银颗粒与阿维A作用组小鼠PASI评分明显降低,症状明显减轻。不同浓度含药血清(2.5%、5%和10%)作用HaCaT细胞后,可抑制其增殖并促进凋亡。此外5%、10%含药血清作用细胞后,能显著抑制JAK、p-JAK、STAT3、p-STAT3蛋白水平。结论中药复方消银颗粒通过抑制JAK/STAT3通路激活在缓解银屑病中发挥重要效应。
文摘Objective： To explore the regulatory mechanism of Xiaoyin Recipe （消银方） on the T helper 1/ T helper 2 （Th1/Th2） immune balance. Methods： Thirty-six experimental animals were divided into three groups, 12 rats in each group： blank control group （B group）, negative control group （N group）, and Xiaoyin Recipe treatment group （T group）. The latter two groups received immunization of experimental autoimmune thyroiditis （EAT）, and T group were treated with Xiaoyin Recipe for a month. Then, the expression of Th1-Th2-related genes in peripheral blood mononuclear cells （PBMCs） were screened with Oligo GEArray Rat Thl-Th2-Th3 Microarray. The expressions of tumor necrosis factor- α （TNF- α）, interleukin-10 （IL-10）, T-box expressed in T-cells （T-bet）, and GATA-binding protein-3 （GATA-3） were detected by real-time polymerase chain reaction （RT-PCR）. Results： Gene array screening showed that compared to N group, in T group after Xiaoyin Recipe treatment, 3 genes were upregulated in EAT rats, including interleukin-27 receptor alpha （IL-27roL）, glomulin （GImn）, and GATA-3, while 38 genes were downregulated, such as CD28, IL-18, signal transducer, and activator of transcription 1 （STAT1）, T-bet, TNF receptor superfamily member 4 （TNFRSF4）, TNF ligand superfamily member 5 （TNFSF5）, and TNF receptor superfamily member 5 （TNFRSF5）. While RT-PCR showed that there was an increased level of TNF-α mRNA （P〈0.01）, an elevated ratio of T-bet/GATA-3, and a decreased level of IL-10 mRNA in PBMC of N and T group compared to B group （P〈0.01）; and after treatment with Xiaoyin Recipe, IL-10 mRNA level increased （P〈0.01）, while TNF-α mRNA level and T-bet/GATA-3 ratio decreased in T group compared to N group （P〈0.01）. Conclusion： Xiaoyin Recipe for psoriasis could induce a Thl/Th2 balance drift toward Th2 in PBMC of EAT rats and thus improve the conditions.
基金Supported by grants from the Chinese National Natural Science Foundation Effect and Action of Cooling Blood and Removing Toxins Traditional Chinese Medicine on New T Cells Subsets and Related Cytokines in Patients with Psoriasis Vulgaris(No.81403403)Immunological Mechanisms Research of Psoriasis Discuss of Traditional Chinese Medicine Based on Imbalance Theory of Helper T Cell(Th)1/Th2,Th17/Regulatory T Cells(Tregs)(No.81403404)+2 种基金The Study on the Relationship Between New T Helper Cells Subsets Th17,Th22,Effect Factor Interleukin(IL)-22 and Syndrome Differentiation Treatment of Psoriasis(No.81273755)2016 Beijing University of Chinese Medicine Foundation for Distinguished Young Scholars Study on The Mechanism of Cooling Blood Detoxification on Different Histopathological Levels of Psoriasis(No.2016-JYB-XJQ005)Young Scientist Development Program 2016,Dong Zhimen Hospital,Affiliated to Beijing University of Chinese Medicine(No.DZMYS-201603)
文摘OBJECTIVE:To evaluate the effectiveness of Xiaoyin Jiedu(XYJD)granules in the treatment of psoriasis vulgaris(PSV)in patients with a blood-heat pattern(BHP)in terms of Traditional Chinese Medicine(TCM).We also aimed to identify the possible underlying immunological mechanism.METHODS:Twenty-five PSV patients with BHP and ten normal controls were enrolled from January 1,2015 to December 31,2016.Patients were randomly assigned to either the XYJD group(15 cases)or the placebo group(10 cases),in which patients were treated with XYJD granules or a placebo,respectively.Additionally,albolene was used to relieve skin dryness in these two groups.The psoriasis area and severity indexes,dermatology life quality indexes and itching scores were assessed at the end of the 2 nd,4 th and 8 th week of treatment.The number of peripheral blood T helper(Th)9,Th17 and regulatory T cells(Tregs)and the m RNA and protein expression levels of PU.1,RAR-related orphan receptor(ROR)-γt,forkhead box protein 3(Foxp3),interleukin(IL)-9,IL-17,IL-23 and IL-10 in the control and experimental groups were compared before and after treatment.RESULTS:Psoriasis area and severity indexes and itching scores of patients in the XYJD group were significantly lower than those in the placebo group,whereas dermatology life quality indexes were significantly higher.In comparison with the placebo group,XYJD granules significantly reduced the number of Th17 cells and the m RNA and protein expression levels of Th17-related ROR-γt,IL-17,IL-22 and IL-23 in the peripheral blood and reduced the number of Th9 cells and the m RNA and protein expression levels of Th9-related PU.1 and IL-9.CONCLUSION:XYJD granules were effective against PSV in patients with BHP by reducing the number of Th9 and Th17 cells and the levels of their related cytokines.
