Five hybridomas stably secreting monoclonal antibodies (McAbs) to androstenedione were prepared by using artificially synthesized androstenedione-11α-succinyl conjugate with bovine serum albumin (BSA) as antigen. The...Five hybridomas stably secreting monoclonal antibodies (McAbs) to androstenedione were prepared by using artificially synthesized androstenedione-11α-succinyl conjugate with bovine serum albumin (BSA) as antigen. These McAbs showed slightly high cross-reactivity with testosterone (8.1—12.3%) and estrone (0.8—2.5%) and high affinities ranging from 2.0×10~7 to 2.8×10~8 L/M. They were all of the IgG_1 subclass. Xinjiang finewool ewes which were passively immunized with McAbs had higher circulating levels of progesterone (P) and luteinizing hormone (LH) than those of control ewes in two oestrous cycles and these changes led to increased ovulation rate and twin lambs born in young ewes.展开更多
采用聚丙烯酰胺凝胶电泳法对青海省三角城种羊场110只引入青海的新疆细毛羊母羊的血清酯酶和血清碱性磷酸酶多态性进行了研究。结果发现:①被检新疆细毛羊血清酯酶基因座有 ESA 和 ESa 两种表型,受一对等位基因 ES^A 和 ES^a 的控制,其...采用聚丙烯酰胺凝胶电泳法对青海省三角城种羊场110只引入青海的新疆细毛羊母羊的血清酯酶和血清碱性磷酸酶多态性进行了研究。结果发现:①被检新疆细毛羊血清酯酶基因座有 ESA 和 ESa 两种表型,受一对等位基因 ES^A 和 ES^a 的控制,其基因频率分别为0.346 3和0.6537;②血清碱性磷酸酶基因座有 ALP B 和 ALP O 两种表型,受一对等位基因ALP^B 和 ALP^O的控制,其基因频率分别为0.292 9和0.707 1③除 ESa 型新疆细毛羊母羊的周岁毛长显著大于 ESA 型羊以外(P<0.01),其它生产性状无 ES 表型间差异;④ALP表型与体重、产毛量和毛长等生产性状之间无显著联系。展开更多
基金Project supported by one of the National Research Subjects in the Seventh Five-Year Science and Technology Plan and the National Natural Science Foundation of China
文摘Five hybridomas stably secreting monoclonal antibodies (McAbs) to androstenedione were prepared by using artificially synthesized androstenedione-11α-succinyl conjugate with bovine serum albumin (BSA) as antigen. These McAbs showed slightly high cross-reactivity with testosterone (8.1—12.3%) and estrone (0.8—2.5%) and high affinities ranging from 2.0×10~7 to 2.8×10~8 L/M. They were all of the IgG_1 subclass. Xinjiang finewool ewes which were passively immunized with McAbs had higher circulating levels of progesterone (P) and luteinizing hormone (LH) than those of control ewes in two oestrous cycles and these changes led to increased ovulation rate and twin lambs born in young ewes.
文摘采用聚丙烯酰胺凝胶电泳法对青海省三角城种羊场110只引入青海的新疆细毛羊母羊的血清酯酶和血清碱性磷酸酶多态性进行了研究。结果发现:①被检新疆细毛羊血清酯酶基因座有 ESA 和 ESa 两种表型,受一对等位基因 ES^A 和 ES^a 的控制,其基因频率分别为0.346 3和0.6537;②血清碱性磷酸酶基因座有 ALP B 和 ALP O 两种表型,受一对等位基因ALP^B 和 ALP^O的控制,其基因频率分别为0.292 9和0.707 1③除 ESa 型新疆细毛羊母羊的周岁毛长显著大于 ESA 型羊以外(P<0.01),其它生产性状无 ES 表型间差异;④ALP表型与体重、产毛量和毛长等生产性状之间无显著联系。
