The Crimean-Congo hemorrhagic fever virus(CCHFV),a member of the genus Orthonairovirus and family Nairoviridae,is transmitted by ticks and causes severe hemorrhagic disease in humans.To study the epidemiology of CCHFV...The Crimean-Congo hemorrhagic fever virus(CCHFV),a member of the genus Orthonairovirus and family Nairoviridae,is transmitted by ticks and causes severe hemorrhagic disease in humans.To study the epidemiology of CCHFV in different ecosystems in Xinjiang,China,a total of 58,932 ticks were collected from Tarim Basin,Junggar Basin,Tianshan Mountain,and Altai Mountain from 2014 to 2017.Hyalomma asiaticum asiaticum was the dominant tick species in Tarim and Junggar basins,whereas Dermacentor nuttalli and Hyalomma detritum were found in Tianshan Mountain and Altai Mountain,respectively.Reverse transcription-polymerase chain reaction of the CCHFV small(S)genome segment was used for the molecular detection.The CCHFV-positive percentage was 5.26%,6.85%,1.94%,and 5.56% in Tarim Basin,Junggar Basin,Tianshan Mountain,and Altai Mountain,respectively.Sequences of the S segment were used for phylogenetic analysis and the results showed that the newly identified CCHFV strains belonged to two clades.Our study confirms that H.asiaticum asiaticum is the major vector of CCHFV in desert habitats which is consistent with previous studies,and also suggests that H.detritum and D.nuttalli are emerging vectors for CCHFV in Xinjiang.Moreover,this study reports the presence of CCHFV in the mountain habitat of Xinjiang for the first time,suggesting that future surveillance of CCHFV should also include mountainous areas.展开更多
On the basis of sequencing and analyzing of the whole M genes(encoding viral membrane antigen glycoprotein) of three Crimean Congo hemorrhagic fever viruses(CCHFV),the Chinese isolates(Xinjiang hemorrhagic fever virus...On the basis of sequencing and analyzing of the whole M genes(encoding viral membrane antigen glycoprotein) of three Crimean Congo hemorrhagic fever viruses(CCHFV),the Chinese isolates(Xinjiang hemorrhagic fever virus,XHFV),we first expressed the glycoprotein (GP) gene of the prototype human origin XHFV strain BA66019 in eukaryotic cells and investigated the expression profiles.Three eukaryotic expression plasmids were constructed starting from ATG 78 (the first start codon of the deduced entire XHFv GP precursor gene positioned between the 78 th ～80 th nucleotides),ATG 93 and ATG 3084 (the potential start codon for G1 precursor gene).The constructs were transfected to COS-7 cells and the expressed products were characterized as membrane-bound proteins which could induce cell fusion This was much more apparent for recombinant plasmid with ATG 3084 A recombinant baculovirus was further created harboring full length GP gene(starting from ATG 78 ) and the expression could also result in the membrane fusion as well as swelling of the infected Sf9 cells The insect cell expressed G1 was smaller in M W than natural G1 (approximately 67kD) on SDS-PAGE and no recombinant G2 band was detectable Western-blot only detected the native G1,while there was no specific corresponding band of recombinant G1 These data suggested that G1 was structurally and functionally important in XHFV and the glycosylation may have great influence on M W as well as antigenicity This study provides a foundation for the future study of viral pathogenesis ,antigenicity and immunity,that are the theoretical and experimental backgrounds for vaccine展开更多
基金the Ministry of Science and Technology of China (2013FY113500)the grants from the National Science Foundation of China (Nos.81460303,81760365)the State Key Laboratory of Virology of China (No.2015IOV003)
文摘The Crimean-Congo hemorrhagic fever virus(CCHFV),a member of the genus Orthonairovirus and family Nairoviridae,is transmitted by ticks and causes severe hemorrhagic disease in humans.To study the epidemiology of CCHFV in different ecosystems in Xinjiang,China,a total of 58,932 ticks were collected from Tarim Basin,Junggar Basin,Tianshan Mountain,and Altai Mountain from 2014 to 2017.Hyalomma asiaticum asiaticum was the dominant tick species in Tarim and Junggar basins,whereas Dermacentor nuttalli and Hyalomma detritum were found in Tianshan Mountain and Altai Mountain,respectively.Reverse transcription-polymerase chain reaction of the CCHFV small(S)genome segment was used for the molecular detection.The CCHFV-positive percentage was 5.26%,6.85%,1.94%,and 5.56% in Tarim Basin,Junggar Basin,Tianshan Mountain,and Altai Mountain,respectively.Sequences of the S segment were used for phylogenetic analysis and the results showed that the newly identified CCHFV strains belonged to two clades.Our study confirms that H.asiaticum asiaticum is the major vector of CCHFV in desert habitats which is consistent with previous studies,and also suggests that H.detritum and D.nuttalli are emerging vectors for CCHFV in Xinjiang.Moreover,this study reports the presence of CCHFV in the mountain habitat of Xinjiang for the first time,suggesting that future surveillance of CCHFV should also include mountainous areas.
文摘On the basis of sequencing and analyzing of the whole M genes(encoding viral membrane antigen glycoprotein) of three Crimean Congo hemorrhagic fever viruses(CCHFV),the Chinese isolates(Xinjiang hemorrhagic fever virus,XHFV),we first expressed the glycoprotein (GP) gene of the prototype human origin XHFV strain BA66019 in eukaryotic cells and investigated the expression profiles.Three eukaryotic expression plasmids were constructed starting from ATG 78 (the first start codon of the deduced entire XHFv GP precursor gene positioned between the 78 th ～80 th nucleotides),ATG 93 and ATG 3084 (the potential start codon for G1 precursor gene).The constructs were transfected to COS-7 cells and the expressed products were characterized as membrane-bound proteins which could induce cell fusion This was much more apparent for recombinant plasmid with ATG 3084 A recombinant baculovirus was further created harboring full length GP gene(starting from ATG 78 ) and the expression could also result in the membrane fusion as well as swelling of the infected Sf9 cells The insect cell expressed G1 was smaller in M W than natural G1 (approximately 67kD) on SDS-PAGE and no recombinant G2 band was detectable Western-blot only detected the native G1,while there was no specific corresponding band of recombinant G1 These data suggested that G1 was structurally and functionally important in XHFV and the glycosylation may have great influence on M W as well as antigenicity This study provides a foundation for the future study of viral pathogenesis ,antigenicity and immunity,that are the theoretical and experimental backgrounds for vaccine
