目的 分析X-pert联合核苷酸结合寡聚化结构域蛋白2(NOD2)、自噬相关蛋白16样蛋白1(ATG16L1)在活动性肺结核患者疾病转归评估中的应用价值。方法 前瞻性选取2023年4月至2024年4月池州市人民医院收治的110例活动性肺结核患者为研究对象。...目的 分析X-pert联合核苷酸结合寡聚化结构域蛋白2(NOD2)、自噬相关蛋白16样蛋白1(ATG16L1)在活动性肺结核患者疾病转归评估中的应用价值。方法 前瞻性选取2023年4月至2024年4月池州市人民医院收治的110例活动性肺结核患者为研究对象。所有患者均行抗结核治疗、疾病转归评估,将转归患者60例作为转归组,未转归患者50例作为未转归组。收集两组患者的临床资料(年龄、体重指数、性别、吸烟史、贫血、累及肺野数、肺部空洞病变、利福平耐药)。治疗前行X-pert、NOD2、ATG16L1检测,比较两组X-pert阳性率及NOD2、ATG16L1表达水平。采用多因素Logistics回归分析分析活动性肺结核患者疾病转归的影响因素,采用受试者操作特征(ROC)曲线分析X-pert、NOD2、ATG16L1对活动性肺结核患者疾病转归的预测价值。结果 两组体重指数、吸烟史、贫血、累及肺野数、利福平耐药比较,差异均无统计学意义(P>0.05);与转归组相比,未转归组患者年龄较大[(56.15±19.34)vs.(63.18±12.84)岁],男性(71.67 vs. 90.00)%、肺部空洞病变(11.67 vs. 32.00)%比例较高,差异均有统计学意义(P<0.05)。与转归组相比,未转归组患者X-pert阳性率(75.00 vs. 90.00)%、NOD2[(164.31±15.55)vs.(199.29±24.63)ng/L]、ATG16L1[(8.95±1.1.74)vs.(12.15±2.26)ng/L]表达水平均较高,差异均有统计学意义(P<0.05)。多因素Logistics回归分析结果显示,年龄、性别、肺部空洞病变、X-pert、NOD2、ATG16L1为活动性肺结核患者疾病转归的危险因素(P<0.05)。与X-pert、NOD2、ATG16L1单项诊断相比,X-pert、NOD2、ATG16L1联合检测对活动性肺结核患者疾病转归的预测价值较高(P<0.05)。结论 疾病未转归活动性肺结核患者X-pert阳性率、NOD2、ATG16L1表达水平均高于转归患者,X-pert、NOD2、ATG16L1为活动性肺结核患者疾病转归的危险因素,X-pert联合NOD2、ATG16L1对活动性肺结核患者疾病转归的预测价值较高,为活动性肺结核患者疾病转归评估提供了有效依据。展开更多
State Administration for Market Regulation and National Standardization Administration of China have approved the following 12 mandatory national standards and 1 mandatory national standard with amendment.
Mitochondrial gene fragments of 16S rRNA gene of four species （Rhacophorus rhodopus, R. reinwardtii, Philautus albopunctatus and P. rhododiscus ） from 11 populations were sequenced in this study. Homologous sequence...Mitochondrial gene fragments of 16S rRNA gene of four species （Rhacophorus rhodopus, R. reinwardtii, Philautus albopunctatus and P. rhododiscus ） from 11 populations were sequenced in this study. Homologous sequences of R. bipunctatus, Theloderma asperum, T. corticale and Buergeria japonica were obtained by screening the GenBank database. After excluding all gaps and ambiguous positions, aligned sequences were 500 bp in length with 115 variable sites and 92 parsimony-informative sites. Using B .japonica as an outgroup, phylogenetic relationships were analyzed using Bayesian inference, maximum parsimony and maximum likelihood methods. Our results indicated that neither of R. rhodopus and P. albopunctatus were monophyletic at the species level. The population of R. rhodopus from Hainan Island was more close to R. bipunctatus than to populations of R. rhodopus from Yunnan Province. Furthermore, the populations of R. rhodopus from Yunnan Province can be divided into two main lineages. Theloderma corticale and P.rhododiscus were clustered together and T. asperum was nested in P.albopunctatus. We considered that P. albopunctatus Liu and Hu, 1962, was the synonymy of T. asperum Boulenger, 1886, and suggested removing P. rhododiscus from Philautus into the genus Theloderma.展开更多
[ Objective] Study on the phylogenetic analysis of the 16S rDNA and insecticidal characteristics of strain BH-1 isolated from diseased larva of Anoplophora glabripennis （Motseh.） [ Method ] The strain was identified...[ Objective] Study on the phylogenetic analysis of the 16S rDNA and insecticidal characteristics of strain BH-1 isolated from diseased larva of Anoplophora glabripennis （Motseh.） [ Method ] The strain was identified by routine method and inoculated onto healthy Anoplophora glabripennis （Motseh.） for observing insecticidal effect, further 16S DNA was amplified by the specific primers for sequencing and homology analysis. [ Result] The mortality of second instar ofAnoplophora glabripennis（ Motseh. ） reached 72.7% 8 d after 10^10cfu/ml BH-1 was inoculated. The homology of 16S DNA sequences between BH-1 and Serratia marcescens accessed in GenBank reached 99.5%. Combined with the results of routine identification, BH-1 was identified as S. marcescens. [Conclusion] BH-1 could be used for biological control ofAnoplophora glabripennis （Motsch.）.展开更多
Objective： To investigate the potential linkage between high rate of p16 methylation and hepatitis B virus （HBV） infection, methylation status of p16, HBV infection markers in serum and HBV-DNA replication level in...Objective： To investigate the potential linkage between high rate of p16 methylation and hepatitis B virus （HBV） infection, methylation status of p16, HBV infection markers in serum and HBV-DNA replication level in cancerous and non-cancerous tissue of 32 cases of hepatocellular carcinomas （HCC） with HBV infection and 12 HCCs without HBV infection were examined. Methods： p16 methylation was detected with methylation-specific polymerase Chain reaction （PCR）, and HBV markers were examined with real-time PCR and immunologic method. Results： Methylation of p16 promoter was found in 31 （70.5%） of 44 cancerous tissues of HCC, 2 （16.7%） of 12 HCC without HBV infection, 29 （90.6%） of 32 HCCs with HBV infection marker, p16 methylation was detected in 5 （83.3%） of 6 HCCs positive for HBsAg and HBeAg, 17 （94.4%） of 18 HCCs positive for HBsAg and negative for HBeAg, 7/8 （87.5%） of HCCs positive for other HBV infection markers, such as HBsAB, HBcAb, HBeAb. p16 methylation products were also found in non-cancerous tissues of 4 cases of HCCs with HBV infection, not detected in non-cancerous tissues without HBV infection. HBV-DNA was detected in cancerous tissues of 29/32 （90%） HCCs with HBV infection. Surprisingly, Methylation product of p16 promoter was found in all cases （29/29） of HCCs with detectable HBV-DNA in neoplastic tissue. Conclusion： Persistent HBV infection may promote p16 hypermethylation, suggesting that HBV, via enhancing the aberrant methylation of p16, indirectly involved in development of HCC.展开更多
文摘目的 分析X-pert联合核苷酸结合寡聚化结构域蛋白2(NOD2)、自噬相关蛋白16样蛋白1(ATG16L1)在活动性肺结核患者疾病转归评估中的应用价值。方法 前瞻性选取2023年4月至2024年4月池州市人民医院收治的110例活动性肺结核患者为研究对象。所有患者均行抗结核治疗、疾病转归评估,将转归患者60例作为转归组,未转归患者50例作为未转归组。收集两组患者的临床资料(年龄、体重指数、性别、吸烟史、贫血、累及肺野数、肺部空洞病变、利福平耐药)。治疗前行X-pert、NOD2、ATG16L1检测,比较两组X-pert阳性率及NOD2、ATG16L1表达水平。采用多因素Logistics回归分析分析活动性肺结核患者疾病转归的影响因素,采用受试者操作特征(ROC)曲线分析X-pert、NOD2、ATG16L1对活动性肺结核患者疾病转归的预测价值。结果 两组体重指数、吸烟史、贫血、累及肺野数、利福平耐药比较,差异均无统计学意义(P>0.05);与转归组相比,未转归组患者年龄较大[(56.15±19.34)vs.(63.18±12.84)岁],男性(71.67 vs. 90.00)%、肺部空洞病变(11.67 vs. 32.00)%比例较高,差异均有统计学意义(P<0.05)。与转归组相比,未转归组患者X-pert阳性率(75.00 vs. 90.00)%、NOD2[(164.31±15.55)vs.(199.29±24.63)ng/L]、ATG16L1[(8.95±1.1.74)vs.(12.15±2.26)ng/L]表达水平均较高,差异均有统计学意义(P<0.05)。多因素Logistics回归分析结果显示,年龄、性别、肺部空洞病变、X-pert、NOD2、ATG16L1为活动性肺结核患者疾病转归的危险因素(P<0.05)。与X-pert、NOD2、ATG16L1单项诊断相比,X-pert、NOD2、ATG16L1联合检测对活动性肺结核患者疾病转归的预测价值较高(P<0.05)。结论 疾病未转归活动性肺结核患者X-pert阳性率、NOD2、ATG16L1表达水平均高于转归患者,X-pert、NOD2、ATG16L1为活动性肺结核患者疾病转归的危险因素,X-pert联合NOD2、ATG16L1对活动性肺结核患者疾病转归的预测价值较高,为活动性肺结核患者疾病转归评估提供了有效依据。
文摘State Administration for Market Regulation and National Standardization Administration of China have approved the following 12 mandatory national standards and 1 mandatory national standard with amendment.
基金Supported by the National Natural Science Foundation of China (30670243)
文摘Mitochondrial gene fragments of 16S rRNA gene of four species （Rhacophorus rhodopus, R. reinwardtii, Philautus albopunctatus and P. rhododiscus ） from 11 populations were sequenced in this study. Homologous sequences of R. bipunctatus, Theloderma asperum, T. corticale and Buergeria japonica were obtained by screening the GenBank database. After excluding all gaps and ambiguous positions, aligned sequences were 500 bp in length with 115 variable sites and 92 parsimony-informative sites. Using B .japonica as an outgroup, phylogenetic relationships were analyzed using Bayesian inference, maximum parsimony and maximum likelihood methods. Our results indicated that neither of R. rhodopus and P. albopunctatus were monophyletic at the species level. The population of R. rhodopus from Hainan Island was more close to R. bipunctatus than to populations of R. rhodopus from Yunnan Province. Furthermore, the populations of R. rhodopus from Yunnan Province can be divided into two main lineages. Theloderma corticale and P.rhododiscus were clustered together and T. asperum was nested in P.albopunctatus. We considered that P. albopunctatus Liu and Hu, 1962, was the synonymy of T. asperum Boulenger, 1886, and suggested removing P. rhododiscus from Philautus into the genus Theloderma.
基金Co-constructed Program of Beijing Education Committee for Scientific Research BaseResearch Fund for the Doctoral Program of Higher Education (20030022015)Program for Changjiang Scholars and Innovative Research Team in University (PCSIRT0607)~~
文摘[ Objective] Study on the phylogenetic analysis of the 16S rDNA and insecticidal characteristics of strain BH-1 isolated from diseased larva of Anoplophora glabripennis （Motseh.） [ Method ] The strain was identified by routine method and inoculated onto healthy Anoplophora glabripennis （Motseh.） for observing insecticidal effect, further 16S DNA was amplified by the specific primers for sequencing and homology analysis. [ Result] The mortality of second instar ofAnoplophora glabripennis（ Motseh. ） reached 72.7% 8 d after 10^10cfu/ml BH-1 was inoculated. The homology of 16S DNA sequences between BH-1 and Serratia marcescens accessed in GenBank reached 99.5%. Combined with the results of routine identification, BH-1 was identified as S. marcescens. [Conclusion] BH-1 could be used for biological control ofAnoplophora glabripennis （Motsch.）.
基金This work was supported by grants from Natural Sciences Fund of Hubei province (2003ABA194)Science Research Fund of Taihe Hospital.
文摘Objective： To investigate the potential linkage between high rate of p16 methylation and hepatitis B virus （HBV） infection, methylation status of p16, HBV infection markers in serum and HBV-DNA replication level in cancerous and non-cancerous tissue of 32 cases of hepatocellular carcinomas （HCC） with HBV infection and 12 HCCs without HBV infection were examined. Methods： p16 methylation was detected with methylation-specific polymerase Chain reaction （PCR）, and HBV markers were examined with real-time PCR and immunologic method. Results： Methylation of p16 promoter was found in 31 （70.5%） of 44 cancerous tissues of HCC, 2 （16.7%） of 12 HCC without HBV infection, 29 （90.6%） of 32 HCCs with HBV infection marker, p16 methylation was detected in 5 （83.3%） of 6 HCCs positive for HBsAg and HBeAg, 17 （94.4%） of 18 HCCs positive for HBsAg and negative for HBeAg, 7/8 （87.5%） of HCCs positive for other HBV infection markers, such as HBsAB, HBcAb, HBeAb. p16 methylation products were also found in non-cancerous tissues of 4 cases of HCCs with HBV infection, not detected in non-cancerous tissues without HBV infection. HBV-DNA was detected in cancerous tissues of 29/32 （90%） HCCs with HBV infection. Surprisingly, Methylation product of p16 promoter was found in all cases （29/29） of HCCs with detectable HBV-DNA in neoplastic tissue. Conclusion： Persistent HBV infection may promote p16 hypermethylation, suggesting that HBV, via enhancing the aberrant methylation of p16, indirectly involved in development of HCC.
