The primary effective components of Xuefuzhuyu, including Rhizoma Chqanxiong, Radix Salviae Miltiorrhiae, and Radix Angelicae Sinensis, have been shown to induce the differentiation of bone marrow mesenchymal stem cel...The primary effective components of Xuefuzhuyu, including Rhizoma Chqanxiong, Radix Salviae Miltiorrhiae, and Radix Angelicae Sinensis, have been shown to induce the differentiation of bone marrow mesenchymal stem cells （BMMSCs） into neuronal-like cells. However, there is little available evidence on the effects of the Chinese herbal compound Xuefuzhuyu on BMMSC differentiation. The present study investigated the effects of Xuefuzhuyu on differentiation of rat BMMSCs into neuronal-like cells, and evaluated the optimal concentration for inducing differentiation. Inverted microscopy was used to observe BMMSCs induced by Xuefuzhuyu; immunocytochemistry revealed expression of the neuronal marker neuron-specific enolase surface antigen in the majority of BMMSCs following treatment with Xuefuzhuyu at concentrations of 1,3, 5 and 10 g/L. A concentration of 3 g/L resulted in highest neuron-specific enolase expression. These results suggest that BMMSCs can be induced to differentiate into neuronal-like cells with Xuefuzhuyu, and 3 g/L is the optimal inductive concentration for effective differentiation.展开更多
Hypoxic-ischemic brain injury models were generated by bilateral carotid artery ligation in Sprague-Dawley rats. Successful models were treated with a combination of Xuefuzhuyu decoction and 10g of astragalus. The exp...Hypoxic-ischemic brain injury models were generated by bilateral carotid artery ligation in Sprague-Dawley rats. Successful models were treated with a combination of Xuefuzhuyu decoction and 10g of astragalus. The experimental results showed that neuronal morphology and structure recovered, nerve growth factor mRNA expression increased in brain tissues, and neurological function signifi-cantly improved. There was no significant difference in these measures compared with rats treated with Xuefuzhuyu decoction alone or a combined treatment of Xuefuzhuyu decoction with 40 g as-tragalus. These experimental findings revealed that, Xuefuzhuyu decoction combined with astra-galus may up-regulate the expression of nerve growth factor mRNA and accordingly exert a neu-roprotective effect; however, this protection is not dependent on astragalus dosage.展开更多
OBJECTIVE:To evaluate the efficacy and safety of Xuefuzhuyu decoction for hyperlipidemia.METHODS:Randomized clinical trials on hyperlipidemia treated by Xuefuzhuyu decoction,either alone or with Western Medicine,were ...OBJECTIVE:To evaluate the efficacy and safety of Xuefuzhuyu decoction for hyperlipidemia.METHODS:Randomized clinical trials on hyperlipidemia treated by Xuefuzhuyu decoction,either alone or with Western Medicine,were searched in electronic databases.Databases searched were:MEDLINE,Allied and Complementary Medicine Database,EMBASE,The Cochrane Library 2013(Issue4),China National Knowledge Infrastructure Database,Chinese Biomedical Literature Database,and Wanfang Database up to 2 May,2013.Study selection,data extraction,quality assessment,and data analysis were conducted according to the Cochranestandards.RESULTS:Six randomized clinical trials involving748 patients(373 patients in the treatment group,375 patients in the control group)were included in the analysis.The studies were of low methodological quality.Meta-analysis indicated that the effect of Xuefuzhuyu decoction on hyperlipidemia was better than that in the control group[n=748,OR=5.07,95%CI(3.40,7.58),P<0.01].Weighted mean differencesin total cholesterol,low-densitylipoprotein cholesterol,triglycerides,and high-density lipoprotein cholesterol were﹣0.79,﹣0.74,﹣0.44,0.16,respectively,and Meta-analysis revealed that the treatment group was better than the control group with 95%CI(﹣1.21,﹣0.36),(﹣0.94,﹣0.55),(﹣0.77,﹣0.11),(0.04,0.27),respectively(all P<0.05).Some adverse events in evaluated studies wererecorded.CONCLUSION:Xuefuzhuyu decoction may be effective for treating hyperlipidemia.The studies we analyzed were of low methodological quality,which indicates that the above findings should be considered cautiously.Therefore,more strictly designed large-scale randomized clinical trials are needed to evaluate the efficacy of Xuefuzhuyu decoction in hyperlipidemia.展开更多
基金the Youth Science Foundation of Affiliated Hospital of Guangdong Medical College,No. 2009k08
文摘The primary effective components of Xuefuzhuyu, including Rhizoma Chqanxiong, Radix Salviae Miltiorrhiae, and Radix Angelicae Sinensis, have been shown to induce the differentiation of bone marrow mesenchymal stem cells （BMMSCs） into neuronal-like cells. However, there is little available evidence on the effects of the Chinese herbal compound Xuefuzhuyu on BMMSC differentiation. The present study investigated the effects of Xuefuzhuyu on differentiation of rat BMMSCs into neuronal-like cells, and evaluated the optimal concentration for inducing differentiation. Inverted microscopy was used to observe BMMSCs induced by Xuefuzhuyu; immunocytochemistry revealed expression of the neuronal marker neuron-specific enolase surface antigen in the majority of BMMSCs following treatment with Xuefuzhuyu at concentrations of 1,3, 5 and 10 g/L. A concentration of 3 g/L resulted in highest neuron-specific enolase expression. These results suggest that BMMSCs can be induced to differentiate into neuronal-like cells with Xuefuzhuyu, and 3 g/L is the optimal inductive concentration for effective differentiation.
基金a grant by Bureau of Traditional Chinese Medicine of Zhejiang Province, No.491040-W50434
文摘Hypoxic-ischemic brain injury models were generated by bilateral carotid artery ligation in Sprague-Dawley rats. Successful models were treated with a combination of Xuefuzhuyu decoction and 10g of astragalus. The experimental results showed that neuronal morphology and structure recovered, nerve growth factor mRNA expression increased in brain tissues, and neurological function signifi-cantly improved. There was no significant difference in these measures compared with rats treated with Xuefuzhuyu decoction alone or a combined treatment of Xuefuzhuyu decoction with 40 g as-tragalus. These experimental findings revealed that, Xuefuzhuyu decoction combined with astra-galus may up-regulate the expression of nerve growth factor mRNA and accordingly exert a neu-roprotective effect; however, this protection is not dependent on astragalus dosage.
基金Supported by National Natural Science Foundation of China(the Inflammatory Mechanism of Renal Damage in Hyperlipidemia Rat After Myocardial Ischemia and the Intevention of Activating Blood and Dissolving Stasis.No.81072924)
文摘OBJECTIVE:To evaluate the efficacy and safety of Xuefuzhuyu decoction for hyperlipidemia.METHODS:Randomized clinical trials on hyperlipidemia treated by Xuefuzhuyu decoction,either alone or with Western Medicine,were searched in electronic databases.Databases searched were:MEDLINE,Allied and Complementary Medicine Database,EMBASE,The Cochrane Library 2013(Issue4),China National Knowledge Infrastructure Database,Chinese Biomedical Literature Database,and Wanfang Database up to 2 May,2013.Study selection,data extraction,quality assessment,and data analysis were conducted according to the Cochranestandards.RESULTS:Six randomized clinical trials involving748 patients(373 patients in the treatment group,375 patients in the control group)were included in the analysis.The studies were of low methodological quality.Meta-analysis indicated that the effect of Xuefuzhuyu decoction on hyperlipidemia was better than that in the control group[n=748,OR=5.07,95%CI(3.40,7.58),P<0.01].Weighted mean differencesin total cholesterol,low-densitylipoprotein cholesterol,triglycerides,and high-density lipoprotein cholesterol were﹣0.79,﹣0.74,﹣0.44,0.16,respectively,and Meta-analysis revealed that the treatment group was better than the control group with 95%CI(﹣1.21,﹣0.36),(﹣0.94,﹣0.55),(﹣0.77,﹣0.11),(0.04,0.27),respectively(all P<0.05).Some adverse events in evaluated studies wererecorded.CONCLUSION:Xuefuzhuyu decoction may be effective for treating hyperlipidemia.The studies we analyzed were of low methodological quality,which indicates that the above findings should be considered cautiously.Therefore,more strictly designed large-scale randomized clinical trials are needed to evaluate the efficacy of Xuefuzhuyu decoction in hyperlipidemia.
