目的探讨血栓通注射液联合奥拉西坦对老年缺血性脑卒中(ischemic stroke,IS)的作用。方法选取106例老年IS患者作为研究对象,随机分为观察组和对照组,每组53例。对照组用奥拉西坦治疗,观察组在对照组治疗的基础上联合血栓通注射液治疗,比...目的探讨血栓通注射液联合奥拉西坦对老年缺血性脑卒中(ischemic stroke,IS)的作用。方法选取106例老年IS患者作为研究对象,随机分为观察组和对照组,每组53例。对照组用奥拉西坦治疗,观察组在对照组治疗的基础上联合血栓通注射液治疗,比较2组的临床疗效、神经功能[用美国国立卫生研究院卒中量表评分(national institute of health stroke scale,NIHSS)评估]、运动功能(用日常生活活动能力(activities of daily living,ADL)量表评估)、颈动脉狭窄程度(颈动脉内中膜厚度和狭窄率)、血流动力学(全血黏度、血流速度和血流阻力)、神经损伤因子[神经胶质原纤维酸性蛋白(glial fibrillary acidic protein,GFAP)、神经元特异性烯醇化酶(neuron-specific enolase,NSE)和脑源性神经生长因子(brain-derived neurotrophic factor,BDNF)]和氧化应激指标[丙二醛(malondialdehyde,MDA)和超氧化物歧化酶(superoxide dismutase,SOD)]水平。结果观察组的总有效率为98.11%,高于对照组的86.79%(P<0.05);治疗后,观察组的NIHSS评分为(10.14±1.91)分,低于对照组的(12.43±2.12)分,P<0.05;观察组的ADL评分为(64.49±8.30)分,高于对照组的(58.37±7.26)分,P<0.05;观察组颈动脉内中膜厚度和狭窄率分别为(1.27±0.11)mm、(74.84%±5.14%),低于对照组[(1.33±0.13)mm、(78.92%±5.95%)],P<0.05;观察组(高切和低切)全血黏度和血流阻力分别为(4.72±0.91)mPa·s、(10.99±2.25)mPa·s、(1.52±0.24)Pa·s·L^(-1),低于对照组[(5.86±1.17)mPa·s、(13.40±2.77)mPa·s、(1.64±0.27)Pa·s·L^(-1)],血流速度为(18.33±3.85)cm·s^(-1),高于对照组[(16.51±3.61)cm·s^(-1)],P<0.05;观察组的GFAP和NSE分别为(5.62±1.11)、(23.88±4.25)ng·mL^(-1),低于对照组[(6.57±1.30)、(27.17±4.76)ng·mL^(-1)],BDNF为(3.06±0.77)ng·mL^(-1),高于对照组[(2.38±0.52)ng·mL^(-1)],P<0.05;观察组的MDA为(3.62±0.63)μmol·L^(-1),低于对照组[(4.05±0.78)μmol·L^(-1)],SOD为(105.20±15.63)U·mL^(-1),高于对照组[(93.42±13.27)U·mL^(-1)],P<0.05。结论血栓通注射液联合奥拉西坦对老年IS患者疗效显著,可通过调节神经损伤因子和氧化应激因子水平及改善血流动力学,提高患者的神经功能和运动功能,改善颈动脉狭窄。展开更多
目的探讨血栓通注射液对急性脑梗死患者脑血流动力学及血清学指标的影响。方法将160例2022年1月-2022年12月伊犁州奎屯医院神经内科收治的急性脑梗死患者随机纳入对照组和试验组,各80例。经脱落或剔除后纳入120例患者进行研究,其中对照...目的探讨血栓通注射液对急性脑梗死患者脑血流动力学及血清学指标的影响。方法将160例2022年1月-2022年12月伊犁州奎屯医院神经内科收治的急性脑梗死患者随机纳入对照组和试验组,各80例。经脱落或剔除后纳入120例患者进行研究,其中对照组50例、试验组70例。对照组给予西医常规治疗,试验组在对照组基础上给予血栓通注射液进行治疗,2组均治疗2周。比较2组治疗2周后的临床疗效;治疗前和治疗2周后的神经功能、自理能力、脑血流动力学、血清学指标及治疗期间的不良反应。结果试验组治疗2周后的总有效率为94.29%,高于对照组的80.00%(P<0.05);治疗2周后,2组美国国立卫生院神经功能缺损评分(National Institutes of Health Stroke Scale,NIHSS)、改良Rankin量表(Modified Rankin Scale,mRS)评分、左侧颈总动脉动态阻力(dynamic resistance,DR)、外周阻力(peripheral resistance,Rv)及血清钙结合蛋白β(calcium-binding proteinβ,S-100β)、神经元特异性烯醇化酶(neuron specific enolase,NSE)、单核细胞趋化蛋白-1(monocyte chemoattractant protein-1,MCP-1)、同型半胱氨酸(homocysteine,Hcy)水平与治疗前比较降低,且试验组低于对照组(P<0.05);2组日常生活活动能力(activities of daily living,ADL)评分及左侧颈总动脉平均血流速率(Vm)、平均血流量(Qm)与治疗前比较升高,且试验组高于对照组(P<0.05)。结论血栓通注射液可明显改善急性脑梗死患者脑血流动力学,调节血清学指标水平变化,进而有助于促进患者神经功能、炎症反应和自理能力的改善,提高临床疗效,且并未明显增加患者的不良反应。展开更多
Objective： Inflammatory reactions induced by microglia in the brain play an important part in the pathogenesis of focal cerebral ischemia/reperfusion （I/R） injury, resulting in neuronal death. Salvianolate Lyophili...Objective： Inflammatory reactions induced by microglia in the brain play an important part in the pathogenesis of focal cerebral ischemia/reperfusion （I/R） injury, resulting in neuronal death. Salvianolate Lyophilized Injection （SLI） and Xueshuantong Injection （Lyophilized） （XST）, which have been widely used in the treatment of acutely cerebral infarction clinically in China, exhibit various biological activities. In this study, the neuroprotective properties of SLI combined with XST in a rat model of middle cerebral artery occlusion- reperfusion （MCAO/R） were investigated. Methods： In this study, male Wistar rats were subjected to 1.5h of middle cerebral artery occlusion followed by reperfusion for 24 h. The rats were randomly divided into the following six groups： normal group （NOR）, model group （MOD）, SLI group （21 mg/kg, SLI）, ）（ST group （100 mg/kg, ）（ST）, SLI combined with XST （XST 100 mg/kg ＋ SLI 21 mg/kg, 1X1S）, and Edaravone （as a positive control drug, 6 mL/kg, EDI）, once a day for 3 d. The neuronal injury, the expression of glial fibrillary acidic protein （GFAP） and ionized calcium binding adaptor molecule 1 （IBA-1）, and the changes of pro-inflammatory mediators interleukin- 6 （IL-6）, tumor necrosis factor alpha （TNF-α） and anti-inflammatory mediator interleukin-10 （IL-10） were observed. Results： 1X1S treatment significantly increased the number of neuron, compared with the MOD group, SH group and XST group. Gliosis （GFAP and IBA-1） and expression of pro-inflammatory mediators IL-6 and TNF-a were significantly reduced. Meanwhile, 1XIS significantly increased the expression of anti- inflammatory mediator IL-10 in the brains of MCAO/R rats, compared with the MOD group, SLI and XST groups. SLI and XST also remarkably down-regulated the expression of IL-6 and TNF-α compared with the MOD group. Conclusions： This study shows that SLI combined with XST （1X1S） can protect cerebral ischemia- reperfusion injury due to its anti-inflammatory property, and may provide a potential promising new therapeutic strategy for acute ischemic stroke.展开更多
Objective To investigate the ameliorate effect and underlying mechanism of Xueshuantong for Injection(Lyophilized,注射用血栓通,XST)in streptozocin(STZ)-induced diabetic retinopathy(DR)rats.Methods Diabetes mellitus(DM...Objective To investigate the ameliorate effect and underlying mechanism of Xueshuantong for Injection(Lyophilized,注射用血栓通,XST)in streptozocin(STZ)-induced diabetic retinopathy(DR)rats.Methods Diabetes mellitus(DM)model was induced by intraperitoneal(i.p.)injection of STZ(60 mg/kg)in Sprague-Dawley rats.Diabetic rats were randomized into 3 groups(n=10)according to a random number table,including DM,XST50 and XST100 groups.XST treatment groups were daily i.p.injected with 50 or 100 mg/kg XST for 60 days,respectively.The control and DM groups were given i.p.injection with saline.Blood glucose level and body weight were recorded every week.Histological changes in the retina tissues were observed with hematoxylin-eosin staining.Apoptosis and inflammation related factors,including cleaved caspase-3,glial fifibrillary acidic protein(GFAP),tumor necrosis factor-α(TNF-α)and intercellular cell adhesion molecule-1(ICAM-1)were detected by Western blot or real-time polymerase chain reaction.Then,the levels of advanced glycation end product(AGE)and its receptor(RAGE)were investigated.Tight junctions proteins(Zonula occludens-1(ZO-1),Occludin and Claudin-5)of blood-retinal barrier were detected by Western blot.The levels of retinal fifibrosis,transforming growth factor-β1(TGF-β1)-Smad2/3 signaling pathway were evaluated at last.Results There was no signifificant difference in the body weight and blood glucose level between XST and DM groups(P>0.05).Compared with the DM group,XST treatment signifificantly increased the retinal thickness of rats(P<0.05 or P<0.01),and suppressed cleaved caspase-3 expression(P<0.01).XST increased the protein expressions of ZO-1,Occludin and Claudin-5 and decreased the mRNA expressions of matrix metalloproteinase 2(MMP-2)and MMP-9(P<0.05 or P<0.01).Moreover,XST signifificantly reduced the productions of AGE and RAGE proteins in the retina of rats(P<0.05 or P<0.01),suppressed the over-expression of TNF-α,and decreased the elevated level of ICAM-1 in retina of rats(P<0.05 or P<0.01).XST signifificantly reduced the levels ofα-smooth muscle actin(α-SMA),connective tissue growth factor(CTGF),TGF-β1 and phosphorylation of Smad2/3 protein in rats(P<0.05 or P<0.01).Conclusions XST had protective effects on DR with possible mechanisms of inhibiting the inflammation and apoptosis,up-regulating the expression of tight junction proteins,suppressing the productions of AGE and RAGE proteins,and blocking the TGF-β/Smad2/3 signaling pathway.XST treatment might play a role for the future therapeutic strategy against DR.展开更多
文摘目的探讨血栓通注射液联合奥拉西坦对老年缺血性脑卒中(ischemic stroke,IS)的作用。方法选取106例老年IS患者作为研究对象,随机分为观察组和对照组,每组53例。对照组用奥拉西坦治疗,观察组在对照组治疗的基础上联合血栓通注射液治疗,比较2组的临床疗效、神经功能[用美国国立卫生研究院卒中量表评分(national institute of health stroke scale,NIHSS)评估]、运动功能(用日常生活活动能力(activities of daily living,ADL)量表评估)、颈动脉狭窄程度(颈动脉内中膜厚度和狭窄率)、血流动力学(全血黏度、血流速度和血流阻力)、神经损伤因子[神经胶质原纤维酸性蛋白(glial fibrillary acidic protein,GFAP)、神经元特异性烯醇化酶(neuron-specific enolase,NSE)和脑源性神经生长因子(brain-derived neurotrophic factor,BDNF)]和氧化应激指标[丙二醛(malondialdehyde,MDA)和超氧化物歧化酶(superoxide dismutase,SOD)]水平。结果观察组的总有效率为98.11%,高于对照组的86.79%(P<0.05);治疗后,观察组的NIHSS评分为(10.14±1.91)分,低于对照组的(12.43±2.12)分,P<0.05;观察组的ADL评分为(64.49±8.30)分,高于对照组的(58.37±7.26)分,P<0.05;观察组颈动脉内中膜厚度和狭窄率分别为(1.27±0.11)mm、(74.84%±5.14%),低于对照组[(1.33±0.13)mm、(78.92%±5.95%)],P<0.05;观察组(高切和低切)全血黏度和血流阻力分别为(4.72±0.91)mPa·s、(10.99±2.25)mPa·s、(1.52±0.24)Pa·s·L^(-1),低于对照组[(5.86±1.17)mPa·s、(13.40±2.77)mPa·s、(1.64±0.27)Pa·s·L^(-1)],血流速度为(18.33±3.85)cm·s^(-1),高于对照组[(16.51±3.61)cm·s^(-1)],P<0.05;观察组的GFAP和NSE分别为(5.62±1.11)、(23.88±4.25)ng·mL^(-1),低于对照组[(6.57±1.30)、(27.17±4.76)ng·mL^(-1)],BDNF为(3.06±0.77)ng·mL^(-1),高于对照组[(2.38±0.52)ng·mL^(-1)],P<0.05;观察组的MDA为(3.62±0.63)μmol·L^(-1),低于对照组[(4.05±0.78)μmol·L^(-1)],SOD为(105.20±15.63)U·mL^(-1),高于对照组[(93.42±13.27)U·mL^(-1)],P<0.05。结论血栓通注射液联合奥拉西坦对老年IS患者疗效显著,可通过调节神经损伤因子和氧化应激因子水平及改善血流动力学,提高患者的神经功能和运动功能,改善颈动脉狭窄。
文摘目的探讨血栓通注射液对急性脑梗死患者脑血流动力学及血清学指标的影响。方法将160例2022年1月-2022年12月伊犁州奎屯医院神经内科收治的急性脑梗死患者随机纳入对照组和试验组,各80例。经脱落或剔除后纳入120例患者进行研究,其中对照组50例、试验组70例。对照组给予西医常规治疗,试验组在对照组基础上给予血栓通注射液进行治疗,2组均治疗2周。比较2组治疗2周后的临床疗效;治疗前和治疗2周后的神经功能、自理能力、脑血流动力学、血清学指标及治疗期间的不良反应。结果试验组治疗2周后的总有效率为94.29%,高于对照组的80.00%(P<0.05);治疗2周后,2组美国国立卫生院神经功能缺损评分(National Institutes of Health Stroke Scale,NIHSS)、改良Rankin量表(Modified Rankin Scale,mRS)评分、左侧颈总动脉动态阻力(dynamic resistance,DR)、外周阻力(peripheral resistance,Rv)及血清钙结合蛋白β(calcium-binding proteinβ,S-100β)、神经元特异性烯醇化酶(neuron specific enolase,NSE)、单核细胞趋化蛋白-1(monocyte chemoattractant protein-1,MCP-1)、同型半胱氨酸(homocysteine,Hcy)水平与治疗前比较降低,且试验组低于对照组(P<0.05);2组日常生活活动能力(activities of daily living,ADL)评分及左侧颈总动脉平均血流速率(Vm)、平均血流量(Qm)与治疗前比较升高,且试验组高于对照组(P<0.05)。结论血栓通注射液可明显改善急性脑梗死患者脑血流动力学,调节血清学指标水平变化,进而有助于促进患者神经功能、炎症反应和自理能力的改善,提高临床疗效,且并未明显增加患者的不良反应。
基金financially supported by the National Natural Science Foundation (81573644)the Natural Science Foundation of Tianjin (14JCYBJC28900)the Tianjin Technology Innovation System and the Condition of Platform Construction Plan (16PTSYJC00120)
文摘Objective： Inflammatory reactions induced by microglia in the brain play an important part in the pathogenesis of focal cerebral ischemia/reperfusion （I/R） injury, resulting in neuronal death. Salvianolate Lyophilized Injection （SLI） and Xueshuantong Injection （Lyophilized） （XST）, which have been widely used in the treatment of acutely cerebral infarction clinically in China, exhibit various biological activities. In this study, the neuroprotective properties of SLI combined with XST in a rat model of middle cerebral artery occlusion- reperfusion （MCAO/R） were investigated. Methods： In this study, male Wistar rats were subjected to 1.5h of middle cerebral artery occlusion followed by reperfusion for 24 h. The rats were randomly divided into the following six groups： normal group （NOR）, model group （MOD）, SLI group （21 mg/kg, SLI）, ）（ST group （100 mg/kg, ）（ST）, SLI combined with XST （XST 100 mg/kg ＋ SLI 21 mg/kg, 1X1S）, and Edaravone （as a positive control drug, 6 mL/kg, EDI）, once a day for 3 d. The neuronal injury, the expression of glial fibrillary acidic protein （GFAP） and ionized calcium binding adaptor molecule 1 （IBA-1）, and the changes of pro-inflammatory mediators interleukin- 6 （IL-6）, tumor necrosis factor alpha （TNF-α） and anti-inflammatory mediator interleukin-10 （IL-10） were observed. Results： 1X1S treatment significantly increased the number of neuron, compared with the MOD group, SH group and XST group. Gliosis （GFAP and IBA-1） and expression of pro-inflammatory mediators IL-6 and TNF-a were significantly reduced. Meanwhile, 1XIS significantly increased the expression of anti- inflammatory mediator IL-10 in the brains of MCAO/R rats, compared with the MOD group, SLI and XST groups. SLI and XST also remarkably down-regulated the expression of IL-6 and TNF-α compared with the MOD group. Conclusions： This study shows that SLI combined with XST （1X1S） can protect cerebral ischemia- reperfusion injury due to its anti-inflammatory property, and may provide a potential promising new therapeutic strategy for acute ischemic stroke.
基金Supported by the National Natural Science Foundation of China(No.81573644)Major New Drugs Innovation and Development(No.2012ZX09101201-004)+1 种基金Guangxi Provincial Department of Science and Technology(No.14125008-2-5)and Tianjin 131 Innovative Team Project and Training Program Foundation for Innovative Research Team of Higher Education in Tianjin(No.TD 13-5050)。
文摘Objective To investigate the ameliorate effect and underlying mechanism of Xueshuantong for Injection(Lyophilized,注射用血栓通,XST)in streptozocin(STZ)-induced diabetic retinopathy(DR)rats.Methods Diabetes mellitus(DM)model was induced by intraperitoneal(i.p.)injection of STZ(60 mg/kg)in Sprague-Dawley rats.Diabetic rats were randomized into 3 groups(n=10)according to a random number table,including DM,XST50 and XST100 groups.XST treatment groups were daily i.p.injected with 50 or 100 mg/kg XST for 60 days,respectively.The control and DM groups were given i.p.injection with saline.Blood glucose level and body weight were recorded every week.Histological changes in the retina tissues were observed with hematoxylin-eosin staining.Apoptosis and inflammation related factors,including cleaved caspase-3,glial fifibrillary acidic protein(GFAP),tumor necrosis factor-α(TNF-α)and intercellular cell adhesion molecule-1(ICAM-1)were detected by Western blot or real-time polymerase chain reaction.Then,the levels of advanced glycation end product(AGE)and its receptor(RAGE)were investigated.Tight junctions proteins(Zonula occludens-1(ZO-1),Occludin and Claudin-5)of blood-retinal barrier were detected by Western blot.The levels of retinal fifibrosis,transforming growth factor-β1(TGF-β1)-Smad2/3 signaling pathway were evaluated at last.Results There was no signifificant difference in the body weight and blood glucose level between XST and DM groups(P>0.05).Compared with the DM group,XST treatment signifificantly increased the retinal thickness of rats(P<0.05 or P<0.01),and suppressed cleaved caspase-3 expression(P<0.01).XST increased the protein expressions of ZO-1,Occludin and Claudin-5 and decreased the mRNA expressions of matrix metalloproteinase 2(MMP-2)and MMP-9(P<0.05 or P<0.01).Moreover,XST signifificantly reduced the productions of AGE and RAGE proteins in the retina of rats(P<0.05 or P<0.01),suppressed the over-expression of TNF-α,and decreased the elevated level of ICAM-1 in retina of rats(P<0.05 or P<0.01).XST signifificantly reduced the levels ofα-smooth muscle actin(α-SMA),connective tissue growth factor(CTGF),TGF-β1 and phosphorylation of Smad2/3 protein in rats(P<0.05 or P<0.01).Conclusions XST had protective effects on DR with possible mechanisms of inhibiting the inflammation and apoptosis,up-regulating the expression of tight junction proteins,suppressing the productions of AGE and RAGE proteins,and blocking the TGF-β/Smad2/3 signaling pathway.XST treatment might play a role for the future therapeutic strategy against DR.
