An experiment was conducted to investigate the effect of dietary supplementation of xylo-oligosaccharides（XOS） on growth performance, meat quality, immune functions, duodenal morphology and intestinal microbial popu...An experiment was conducted to investigate the effect of dietary supplementation of xylo-oligosaccharides（XOS） on growth performance, meat quality, immune functions, duodenal morphology and intestinal microbial populations of broilers fed a conventional corn-soybean meal basal diet. A total of 450 1-day-old commercial Arbor Acres male broiler chicks were randomly allocated by bodyweight to 1 of 5 treatments with 6 replicate cages（15 broilers per cage） for each of 5 treatments in a completely randomized design. Chicks were fed the basal corn-soybean meal diets supplemented with 0, 25, 50, 75, or 100 mg of XOS kg–1 of diet, respectively, for an experimental duration of 42 days. The results showed that supplementation of XOS affected feed conversion rate（feed/gain, F/G） during days 22–42 and 1–42（P〈0.03）, drip loss in thigh muscle（P=0.02）, and duodenal crypt depth（P=0.005） on day 42, but had no effect（P〉0.05） on all other measured indices. The chicks fed the diet supplemented with 100 mg of XOS kg–1 had the lowest（P〈0.05） F/G and drip loss in thigh muscle. The drip loss in thigh muscle decreased linearly（P=0.003） as the supplemented XOS increased. Duodenal crypt depth decreased（P〈0.05） at the supplemental level of 75 mg of XOS kg–1. The results indicate that dietary supplementations of 75 and 100 mg of XOS kg–1 are beneficial to broilers fed a conventional corn-soybean meal diet.展开更多
The preparation of xylo-oligosaccharides(XOSs)through hydrolysis of hemicelluloses was studied.The hemicelluloses were isolated from the press lye discharged in the production of viscose,which contained about 30%xylan...The preparation of xylo-oligosaccharides(XOSs)through hydrolysis of hemicelluloses was studied.The hemicelluloses were isolated from the press lye discharged in the production of viscose,which contained about 30%xylan.Then,a factorial experimental design was applied to compare the influences of several factors including the concentrations of sulphuric acid and hemicelluloses,the duration and temperature of the hydrolysis,on the conversion of xylan,and the selectivity for the product–XOSs.The results showed that the hydrolysis duration affects the yield of XOSs to the greatest extent.It is difficult to obtain a high yield of XOSs with sulphuric acid as the hydrolysis catalyst.展开更多
The time course of xylo-oligosaccharides concentration and xylo-oligosaccharides yield in the separation of xylo-oligosaccharides from enzymatic hydrolytes was studied using a membrane reactor with constant permeate f...The time course of xylo-oligosaccharides concentration and xylo-oligosaccharides yield in the separation of xylo-oligosaccharides from enzymatic hydrolytes was studied using a membrane reactor with constant permeate flux of 4 L·m -2 ·h -1 . The results show that xylanases retain 90% of its activity in the reactor. The concentration of xylo-oligosaccharides achieves the maximum, about 5.48 g/L in 30 min. The difference of xylo-oligosaccharides in the retentate and permeate stream is low, <0.62 g/L, therefore it can permeate through membrane. Under the operating conditions that xylan concentration is 30.0 g/L, pH 5.0, operating pressure 16 kPa, temperature 48 ℃, feed velocity 400 mL/min, reaction volume 400 mL, enzyme dosage 10%(volume fraction), dilution rate 1 h -1 , and enzymatic hydrolysis time 195 min, the yield of xylo-oligosaccharides reaches 31.69%.展开更多
The emerging food additive, xylo-oligosaccharide(XOSs), was prepared through enzymatic hydrolysis of hemicelluloses isolated from press lye. Two of the three experimental enzyme preparations presented favourable effec...The emerging food additive, xylo-oligosaccharide(XOSs), was prepared through enzymatic hydrolysis of hemicelluloses isolated from press lye. Two of the three experimental enzyme preparations presented favourable effects, while the other one consisting of the nominal enzyme preparations with high enzyme activity was found to have poor catalytic effects. The conversion of xylan exceeded 40% and the selectivity for XOSs reached 90% when the temperature, hemicellulose concentration, dosage of enzymes, and hydrolysis time were 40?C, 100 g/L, 1 ku/g, and 4 h, respectively. Xylo-oligosaccharide preparation through enzymatic hydrolysis of hemicelluloses isolated from press lye was proved to be a feasible process to utilize the by-product of the lye regeneration, which will substantially improve the economy of the lye regeneration and recycling.展开更多
Properties of enzyme production of a Bacillus subtitles strain （ MSJ-5 ） isolated from soil were studied in the test. Enzyme production reached the peak when MSJ-5 being cultured in fermentation medium for 32 h. 6-1...Properties of enzyme production of a Bacillus subtitles strain （ MSJ-5 ） isolated from soil were studied in the test. Enzyme production reached the peak when MSJ-5 being cultured in fermentation medium for 32 h. 6-1,4-D-mannanase was the main component of crude enzyme solution, test results showed that the optimal reaction temperature of the enzyme was 50 ℃, the optimum reaction pH was 7.0, and the enzyme was stable when pH was 5.0 -7.0. Hydrolysis test re- suits showed that β-1,4-D-mannanase produced by MSJ-5 had significant viscosity reduction effects on Konjak mannan, and the major hydrolysis product was man- nan oligosaecharide. The results indicated that 6-1,4-D-mannanase produced by B. subtilis MSJ-5 had application potential in feed ingredients and functional oligo- saccharides industry.展开更多
基金supported by the Shandong Longlive Bio-Technology Co. Ltd., Chinathe Agricultural Science and Technology Innovation Program, China (ASTIP-IAS08)+1 种基金the Special Fund for Agro-Scientific Research in the Public Interest, China (201403047)the China Agriculture Research System (CARS-42)
文摘An experiment was conducted to investigate the effect of dietary supplementation of xylo-oligosaccharides（XOS） on growth performance, meat quality, immune functions, duodenal morphology and intestinal microbial populations of broilers fed a conventional corn-soybean meal basal diet. A total of 450 1-day-old commercial Arbor Acres male broiler chicks were randomly allocated by bodyweight to 1 of 5 treatments with 6 replicate cages（15 broilers per cage） for each of 5 treatments in a completely randomized design. Chicks were fed the basal corn-soybean meal diets supplemented with 0, 25, 50, 75, or 100 mg of XOS kg–1 of diet, respectively, for an experimental duration of 42 days. The results showed that supplementation of XOS affected feed conversion rate（feed/gain, F/G） during days 22–42 and 1–42（P〈0.03）, drip loss in thigh muscle（P=0.02）, and duodenal crypt depth（P=0.005） on day 42, but had no effect（P〉0.05） on all other measured indices. The chicks fed the diet supplemented with 100 mg of XOS kg–1 had the lowest（P〈0.05） F/G and drip loss in thigh muscle. The drip loss in thigh muscle decreased linearly（P=0.003） as the supplemented XOS increased. Duodenal crypt depth decreased（P〈0.05） at the supplemental level of 75 mg of XOS kg–1. The results indicate that dietary supplementations of 75 and 100 mg of XOS kg–1 are beneficial to broilers fed a conventional corn-soybean meal diet.
基金finically supported by Science and Education Integration Program of Henan University of Technology
文摘The preparation of xylo-oligosaccharides(XOSs)through hydrolysis of hemicelluloses was studied.The hemicelluloses were isolated from the press lye discharged in the production of viscose,which contained about 30%xylan.Then,a factorial experimental design was applied to compare the influences of several factors including the concentrations of sulphuric acid and hemicelluloses,the duration and temperature of the hydrolysis,on the conversion of xylan,and the selectivity for the product–XOSs.The results showed that the hydrolysis duration affects the yield of XOSs to the greatest extent.It is difficult to obtain a high yield of XOSs with sulphuric acid as the hydrolysis catalyst.
文摘The time course of xylo-oligosaccharides concentration and xylo-oligosaccharides yield in the separation of xylo-oligosaccharides from enzymatic hydrolytes was studied using a membrane reactor with constant permeate flux of 4 L·m -2 ·h -1 . The results show that xylanases retain 90% of its activity in the reactor. The concentration of xylo-oligosaccharides achieves the maximum, about 5.48 g/L in 30 min. The difference of xylo-oligosaccharides in the retentate and permeate stream is low, <0.62 g/L, therefore it can permeate through membrane. Under the operating conditions that xylan concentration is 30.0 g/L, pH 5.0, operating pressure 16 kPa, temperature 48 ℃, feed velocity 400 mL/min, reaction volume 400 mL, enzyme dosage 10%(volume fraction), dilution rate 1 h -1 , and enzymatic hydrolysis time 195 min, the yield of xylo-oligosaccharides reaches 31.69%.
基金Supported by Science and Education Integration Program of Henan University of Technology
文摘The emerging food additive, xylo-oligosaccharide(XOSs), was prepared through enzymatic hydrolysis of hemicelluloses isolated from press lye. Two of the three experimental enzyme preparations presented favourable effects, while the other one consisting of the nominal enzyme preparations with high enzyme activity was found to have poor catalytic effects. The conversion of xylan exceeded 40% and the selectivity for XOSs reached 90% when the temperature, hemicellulose concentration, dosage of enzymes, and hydrolysis time were 40?C, 100 g/L, 1 ku/g, and 4 h, respectively. Xylo-oligosaccharide preparation through enzymatic hydrolysis of hemicelluloses isolated from press lye was proved to be a feasible process to utilize the by-product of the lye regeneration, which will substantially improve the economy of the lye regeneration and recycling.
基金Supported by National Public Welfare Industry(Agriculture)Scientific Special Project(201203001)Shandong Agricultural Sciences Youth Fund Project(2014QNM21)Shandong Independent Innovation Achievements Special(2013ZHZX2A0402)
文摘Properties of enzyme production of a Bacillus subtitles strain （ MSJ-5 ） isolated from soil were studied in the test. Enzyme production reached the peak when MSJ-5 being cultured in fermentation medium for 32 h. 6-1,4-D-mannanase was the main component of crude enzyme solution, test results showed that the optimal reaction temperature of the enzyme was 50 ℃, the optimum reaction pH was 7.0, and the enzyme was stable when pH was 5.0 -7.0. Hydrolysis test re- suits showed that β-1,4-D-mannanase produced by MSJ-5 had significant viscosity reduction effects on Konjak mannan, and the major hydrolysis product was man- nan oligosaecharide. The results indicated that 6-1,4-D-mannanase produced by B. subtilis MSJ-5 had application potential in feed ingredients and functional oligo- saccharides industry.
