Occlusion of vas deferens in uitro and in vivo with Nd2+ YAG laser of 100mW-1000mW was investigated. An optical fiber core in diameter of 200pm was inserted into the lumen of the uas uia a canula in & rabbits and ...Occlusion of vas deferens in uitro and in vivo with Nd2+ YAG laser of 100mW-1000mW was investigated. An optical fiber core in diameter of 200pm was inserted into the lumen of the uas uia a canula in & rabbits and 16 uasa deferens were irradiated in uiuo.The threshold lesion(denned as a half thickness of the uas wall being penetrated)at dijferent power and exposure duration was investigated in uitro. Temperature on the aduentitia was also determined.The uasa delerens were totally occluded in those irradiated with power of 800mW for 24 sec or with power of 1000mW for 16 sec 3 and 4 weeks after irradiation.The authors considered that it would be possible to insert percutaneously a fiber and coagulate vas deferens with laser for sterilization in the near future. However,more investigations are needed before it could be employed in the clinic.展开更多
<abstract>Aim: To study the histologic changes of the vas deferens following Nd: YAG laser irradiation. Methods: Intravasal laser irradiation was given to (i) 52 segments of rabbit (laser dosage: 2 seconds at 40...<abstract>Aim: To study the histologic changes of the vas deferens following Nd: YAG laser irradiation. Methods: Intravasal laser irradiation was given to (i) 52 segments of rabbit (laser dosage: 2 seconds at 40 W-50 W) and 16 segments of human (3 seconds at 45 W-55 W) vas deferens in vitro, (ii) 25 rabbit vasa (2 seconds-2.5 seconds at 40 W-45 W) in vivo and (iii) 2 human vasa (3 seconds at 55W) in vivo. Segments of vasa were removed from the in vivo irradiated vasa deferentia 15 days-180 days (rabbit) or 15 days (man) after the exposure. All vas segments were embedded in methacrylate resin. Serial sections (thickness 25μm-30μm) were obtained and observed under a light microscope. Results: (i) Laser-induced damage reached the muscularis layer in 27 % and 94 % of the rabbit and human vas segments in vitro, respectively, (ii) Fourteen of the 25 in vivo rabbit vasa were completely occluded by fibrous tissue and the longer the time interval after treatment, the more likely was the vas occluded. Those unoccluded vasa had either a normal histology or a mucosal damage, (iii) One in vivo human vas was almost completely occluded by the fibrous tissue but the other had a relatively large lumen packed with sperm granulomatous tissue and partial destruction of the smooth muscle layer. Conclusion: Laser irradiation can induce long-term vas occlusion; for rapid occlusion, laser doses just completely destroying the mucosal layer will be advisable.展开更多
文摘Occlusion of vas deferens in uitro and in vivo with Nd2+ YAG laser of 100mW-1000mW was investigated. An optical fiber core in diameter of 200pm was inserted into the lumen of the uas uia a canula in & rabbits and 16 uasa deferens were irradiated in uiuo.The threshold lesion(denned as a half thickness of the uas wall being penetrated)at dijferent power and exposure duration was investigated in uitro. Temperature on the aduentitia was also determined.The uasa delerens were totally occluded in those irradiated with power of 800mW for 24 sec or with power of 1000mW for 16 sec 3 and 4 weeks after irradiation.The authors considered that it would be possible to insert percutaneously a fiber and coagulate vas deferens with laser for sterilization in the near future. However,more investigations are needed before it could be employed in the clinic.
文摘<abstract>Aim: To study the histologic changes of the vas deferens following Nd: YAG laser irradiation. Methods: Intravasal laser irradiation was given to (i) 52 segments of rabbit (laser dosage: 2 seconds at 40 W-50 W) and 16 segments of human (3 seconds at 45 W-55 W) vas deferens in vitro, (ii) 25 rabbit vasa (2 seconds-2.5 seconds at 40 W-45 W) in vivo and (iii) 2 human vasa (3 seconds at 55W) in vivo. Segments of vasa were removed from the in vivo irradiated vasa deferentia 15 days-180 days (rabbit) or 15 days (man) after the exposure. All vas segments were embedded in methacrylate resin. Serial sections (thickness 25μm-30μm) were obtained and observed under a light microscope. Results: (i) Laser-induced damage reached the muscularis layer in 27 % and 94 % of the rabbit and human vas segments in vitro, respectively, (ii) Fourteen of the 25 in vivo rabbit vasa were completely occluded by fibrous tissue and the longer the time interval after treatment, the more likely was the vas occluded. Those unoccluded vasa had either a normal histology or a mucosal damage, (iii) One in vivo human vas was almost completely occluded by the fibrous tissue but the other had a relatively large lumen packed with sperm granulomatous tissue and partial destruction of the smooth muscle layer. Conclusion: Laser irradiation can induce long-term vas occlusion; for rapid occlusion, laser doses just completely destroying the mucosal layer will be advisable.
