青岛老鹳草花粉和花芽扫描电镜制样条件探讨

为了探究与老鹳草相关类型花粉花芽的样品电镜制备,采用直接观察法和戊二醛固定冷冻干燥法对青岛老鹳草花粉扫描电镜制样条件进行了研究。结果表明:直接观察法制备的青岛老鹳草花粉颗粒在扫描电镜下形态饱满,结构清晰,花粉表面的纹饰和突起微观结构清晰完整;改进的FAA单固定冷冻干燥法处理的青岛老鹳草花芽形态学结构完整,层次丰富,立体感强,细胞失水皱缩得到明显改善。通过对不同制样方法进行比较,最终得到可靠简单易行的样品制备方法,对牻牛儿苗科植物的研究及相关类型花粉花芽的样品制备有重要的指导意义。

Araucarian cone-scale complexes,newly found in Aptian Kitadani Formation of Tetori Group in Fukui Prefecture,Central Japan

This study reports on two forms of Araucarian cone-scale complexes from the Aptian Kitadani Formation of the Tetori Group in Fukui Prefecture,Central Japan.One of these has been identified as a new fossil species:Araucarites kitadaniensis Yabe et Yukawa sp.nov.This is the first known occurrence of Araucarian macrofossils from the Tetori Group,indicating that this group was substantially diverse during that time period.This finding concurs with previous palynological investigations at the site,supporting the hypothesis that Araucarian plants began to diversify during Aptian global warming.

K氏酵母与清酒酵母产孢率的研究及原生质体制备

本文考察了Ｋ氏酿酒酵母（ＳａｃｃｈａｒｏｍｙｃｅｓｃｅｒｅｖｉｓｉａｅＫ）与清酒酵母ＳａｃｃｈａｒｏｍｙｃｅｓｓａｋｅＹａｂｅ的最适产孢前培养条件与最适产孢培养条件，以及二者原生质体的制备条件，并对二者的融合进行了初步的研究，试验表明：二供试菌经产孢前培养基Ⅱ和１号产孢培养基培养可获得较高产孢率；并且最适产孢温度为２８℃；用２％蜗牛酶于３１℃酶解３ｈｒ，可除去子囊壁，收集原生质体；用４％蜗牛酶，３３℃酶解２－３ｈｒ，制备原生质体；进一步试验表明，Ｋ氏酿酒酵母与清洒酵母可以进行原生质体融合，且得到一融合子Ｆ＿１．