The milk protein polymorphisms were typed by polyacrylamide gel electrophoresis (PAGE)from 109 Maiwa and 100 Jiulong yaks, and the relationships among milk protein polymorphisms,milking traits and milk protein composi...The milk protein polymorphisms were typed by polyacrylamide gel electrophoresis (PAGE)from 109 Maiwa and 100 Jiulong yaks, and the relationships among milk protein polymorphisms,milking traits and milk protein compositions were studied. The results showed thatβ-CN,κ-CN andα-La were monomorphic,αs1-CN andβ-Lg were polymorphic, the dominantgenes were αs1-CN D and β-Lg E,respectively. The frequencies of αs1-CN D were 0.8073and 0.6000 and β-Lg E were 0.9770 and 0.9700 in two populations respectively.The meanheterozygosities were 0.1021 and 0.1867 in two populations. No significant effects onmilking traits and milk protein compositions were observed except for αs1-CN locus onfat percentage in Jiulong yak.展开更多
Endothelial PAS domain protein 1 gene （EPAS1） is a key transcription factor that activates the expression of oxygen-regu- lated genes. In this study, in order to better understand the effects of EPAS1 gene on hemato...Endothelial PAS domain protein 1 gene （EPAS1） is a key transcription factor that activates the expression of oxygen-regu- lated genes. In this study, in order to better understand the effects of EPAS1 gene on hematologic parameters in yak, we firstly quantified the tissue expression patterns for EPASl mRNA of yak, identified polymorphism in this gene and evaluated its association with hematologic parameters. Expression of EPAS1 mRNA was detected in all eight tissues （heart, liver, lung, spleen, pancreas, kidney, muscles and ovary）. The expressions of EPAS1 in lung and pancreas were extremely higher than other tissues examined. Three novel single nucleotide polymorphisms （SNPs） （g.83052 C〉T, g.83065 G〉A and g.83067 C〉A） within the EPAS1 were identified and genotyped in Pali （PL）, Gannan （GN） and Tianzhu White （TZW） yak breeds. Significant higher frequencies of the AA and GA genotypes and A allele of the g.83065 G〉A were observed in the PL and GN breeds than that in the TZW breed （P〈0.01）. Association analysis of the PL breed indicated that the g.83065 G〉A polymorphism was significantly associated with hemoglobin （HGB） concentration in yaks （P〈0.05）. Individuals with genotype AA had significantly higher HGB concentration （P〈0.05） than those with genotype GA and GG. All these results will help our further understanding of biological functional of yak EPAS1 gene in responding to hypoxia and also indicate EPAS1 might contribute to the hypoxia adaptation of the yak.展开更多
Objective:To detect single nucleotide polymorphisms(SNPs)in the cod transferrin gene by comparing the sequences from Norwegian(North East Atlantic Ocean)and Canadian(North West Atlantic Ocean)specimen,and to quantify ...Objective:To detect single nucleotide polymorphisms(SNPs)in the cod transferrin gene by comparing the sequences from Norwegian(North East Atlantic Ocean)and Canadian(North West Atlantic Ocean)specimen,and to quantify the genetic variation and differentiation in East and West Atlantic cod populations.Methods:cDNA sequences between individuals of Canadian(North West Atlantic Ocean)and Norwegian(North East Atlantic Ocean)origin were aligned.Allele frequencies of the SNPs were used to discriminate the different Atlantic cod populations in West/East Atlantic Ocean,and the Baltic Sea.Results:The sequence alignment detected 19 SNPs,of which 18 of them resulted in amino acid changes in the transferrin protein.Nonsynonymous to synonymous site substitution ratio(dn/ds)was by far greater than 1 providing an evidence for the existence of positive selection.The West Atlantic cod populations showed high values of heterozygosity and the Baltic populations were found to be inbred.Conclusions:This study identified and indicated transferrin gene polymorphisms that can be used for population differentiations.展开更多
Transferrin is a single monomeric glycoprotein of molecular weight of 80 kDa that transports iron involved in many metabolic processes amongst the sites of absorption,storage and utilization,hence considered as the ma...Transferrin is a single monomeric glycoprotein of molecular weight of 80 kDa that transports iron involved in many metabolic processes amongst the sites of absorption,storage and utilization,hence considered as the major iron binding protein in the plasma of vertebrate species.In this study,transferrin structure,synthesis,receptor and the mechanism of cellular uptake of iron from transferrin have been reviewed.Besides,the major biological functions of transferrin and the different forms of it(polymorphisms)have been indicated.展开更多
基金supported by the Nationa1 Natura1 Science Foundation of China(39870607).
文摘The milk protein polymorphisms were typed by polyacrylamide gel electrophoresis (PAGE)from 109 Maiwa and 100 Jiulong yaks, and the relationships among milk protein polymorphisms,milking traits and milk protein compositions were studied. The results showed thatβ-CN,κ-CN andα-La were monomorphic,αs1-CN andβ-Lg were polymorphic, the dominantgenes were αs1-CN D and β-Lg E,respectively. The frequencies of αs1-CN D were 0.8073and 0.6000 and β-Lg E were 0.9770 and 0.9700 in two populations respectively.The meanheterozygosities were 0.1021 and 0.1867 in two populations. No significant effects onmilking traits and milk protein compositions were observed except for αs1-CN locus onfat percentage in Jiulong yak.
基金supported by the Special Fund for Agro-scientific Research in the Public Interest,China (201003061)the Key Technologies R&D Program of China during the 12thFive-Year Plan period (2012BAD13B05)+1 种基金the Great Project of Science and Technology of Gansu Province in China (1102NKDA027)the National Natural Science Foundation of China (31101702)
文摘Endothelial PAS domain protein 1 gene （EPAS1） is a key transcription factor that activates the expression of oxygen-regu- lated genes. In this study, in order to better understand the effects of EPAS1 gene on hematologic parameters in yak, we firstly quantified the tissue expression patterns for EPASl mRNA of yak, identified polymorphism in this gene and evaluated its association with hematologic parameters. Expression of EPAS1 mRNA was detected in all eight tissues （heart, liver, lung, spleen, pancreas, kidney, muscles and ovary）. The expressions of EPAS1 in lung and pancreas were extremely higher than other tissues examined. Three novel single nucleotide polymorphisms （SNPs） （g.83052 C〉T, g.83065 G〉A and g.83067 C〉A） within the EPAS1 were identified and genotyped in Pali （PL）, Gannan （GN） and Tianzhu White （TZW） yak breeds. Significant higher frequencies of the AA and GA genotypes and A allele of the g.83065 G〉A were observed in the PL and GN breeds than that in the TZW breed （P〈0.01）. Association analysis of the PL breed indicated that the g.83065 G〉A polymorphism was significantly associated with hemoglobin （HGB） concentration in yaks （P〈0.05）. Individuals with genotype AA had significantly higher HGB concentration （P〈0.05） than those with genotype GA and GG. All these results will help our further understanding of biological functional of yak EPAS1 gene in responding to hypoxia and also indicate EPAS1 might contribute to the hypoxia adaptation of the yak.
文摘Objective:To detect single nucleotide polymorphisms(SNPs)in the cod transferrin gene by comparing the sequences from Norwegian(North East Atlantic Ocean)and Canadian(North West Atlantic Ocean)specimen,and to quantify the genetic variation and differentiation in East and West Atlantic cod populations.Methods:cDNA sequences between individuals of Canadian(North West Atlantic Ocean)and Norwegian(North East Atlantic Ocean)origin were aligned.Allele frequencies of the SNPs were used to discriminate the different Atlantic cod populations in West/East Atlantic Ocean,and the Baltic Sea.Results:The sequence alignment detected 19 SNPs,of which 18 of them resulted in amino acid changes in the transferrin protein.Nonsynonymous to synonymous site substitution ratio(dn/ds)was by far greater than 1 providing an evidence for the existence of positive selection.The West Atlantic cod populations showed high values of heterozygosity and the Baltic populations were found to be inbred.Conclusions:This study identified and indicated transferrin gene polymorphisms that can be used for population differentiations.
基金supported by the Ethiopian Biodiversity Institute.
文摘Transferrin is a single monomeric glycoprotein of molecular weight of 80 kDa that transports iron involved in many metabolic processes amongst the sites of absorption,storage and utilization,hence considered as the major iron binding protein in the plasma of vertebrate species.In this study,transferrin structure,synthesis,receptor and the mechanism of cellular uptake of iron from transferrin have been reviewed.Besides,the major biological functions of transferrin and the different forms of it(polymorphisms)have been indicated.
