This study isolated and purified strain 431 from an animal probiotic product.Through staining and microscopic examination,colony morphology analysis,biochemical reaction tests,and 16S rDNA sequence alignment,the strai...This study isolated and purified strain 431 from an animal probiotic product.Through staining and microscopic examination,colony morphology analysis,biochemical reaction tests,and 16S rDNA sequence alignment,the strain was identified and named Brevibacterium aureus 431.The study focused on the production of biosurfactants by strain 431,and antibacterial activity tests were conducted on the strain and its secondary metabolites.The results showed that strain 431 exhibited no resistance to 10 commonly used drugs,and its concentrated secondary metabolites were highly sensitive to the indicator bacterium Escherichia coli.Oral administration of strain 431 to BALB/c mice resulted in normal mental state,diet,and bowel movements,with no signs of illness or death,indicating that strain 431 is highly safe and non-pathogenic to mice.The study suggests that Brevibacterium aureus 431 has significant research value as a new source of actinomycetes and that its secondary metabolites have potential application value in the development of antibacterial drugs.展开更多
目的研究亚甲蓝介导的光动力疗法对人皮肤鳞状细胞癌A431细胞株的杀伤作用以及其诱导凋亡的机制,为亚甲蓝光动力临床应用提供理论依据以及最佳治疗参数。方法采用亚甲蓝与人皮肤鳞状细胞癌A431细胞株共同孵育,经630 nm红光照射。采用CC...目的研究亚甲蓝介导的光动力疗法对人皮肤鳞状细胞癌A431细胞株的杀伤作用以及其诱导凋亡的机制,为亚甲蓝光动力临床应用提供理论依据以及最佳治疗参数。方法采用亚甲蓝与人皮肤鳞状细胞癌A431细胞株共同孵育,经630 nm红光照射。采用CCK-8法检测细胞生长的抑制率,确定培育时间、亚甲蓝浓度对A431细胞株的抑制作用。采用免疫组化法检测A431细胞在亚甲蓝光动力作用前后细胞色素c(Cyt-c)在细胞内的分布情况。最后,采用Western-blot方法检测亚甲蓝光动力治疗后细胞凋亡过程中,线粒体凋亡途径相关蛋白Bcl-2和Bax的表达情况。结果CCK8法检测结果显示亚甲蓝光动力组对A431细胞具有杀伤作用,其杀伤作用与亚甲蓝的药物浓度以及亚甲蓝光动力作用后的孵育时间具有密切相关。Cyt-c免疫荧光结果显示亚甲蓝光光动力作用后,细胞凋亡率空白对照0%,光照对照组8%,亚甲蓝对照组12%,光照后1 h 22%,光照后2 h 26%,光照后4 h 31%,光照后6 h 54%。Western blot的检测结果显示亚甲蓝光动力作用激活A431细胞线粒体凋亡途径,启动Bcl-2家族参与凋亡过程。随着亚甲蓝光动力作用后孵育时间的延长,促凋亡蛋白Bax上调,抗凋亡蛋白Bcl-2下调。结论亚甲蓝光动力能明显抑制A431细胞的增殖,并诱导其发生凋亡。凋亡的发生与启动细胞色素c相关的线粒体凋亡途径相关。展开更多
基金Self-funded Science and Technology Innovation Project of Foshan City(220001005797)Foshan University Innovation and Entrepreneurship Training Program for College Students in 2023+2 种基金Foshan City Social Science Planning Project(2024-GJ037)Basic and Applied Basic Research Foundation of Guangdong Province(2022A1515140052)Innovation Project of Guangdong Graduate Education(2022JGXM129,2022JGXM128,2023ANLK-080)。
文摘This study isolated and purified strain 431 from an animal probiotic product.Through staining and microscopic examination,colony morphology analysis,biochemical reaction tests,and 16S rDNA sequence alignment,the strain was identified and named Brevibacterium aureus 431.The study focused on the production of biosurfactants by strain 431,and antibacterial activity tests were conducted on the strain and its secondary metabolites.The results showed that strain 431 exhibited no resistance to 10 commonly used drugs,and its concentrated secondary metabolites were highly sensitive to the indicator bacterium Escherichia coli.Oral administration of strain 431 to BALB/c mice resulted in normal mental state,diet,and bowel movements,with no signs of illness or death,indicating that strain 431 is highly safe and non-pathogenic to mice.The study suggests that Brevibacterium aureus 431 has significant research value as a new source of actinomycetes and that its secondary metabolites have potential application value in the development of antibacterial drugs.
文摘目的研究亚甲蓝介导的光动力疗法对人皮肤鳞状细胞癌A431细胞株的杀伤作用以及其诱导凋亡的机制,为亚甲蓝光动力临床应用提供理论依据以及最佳治疗参数。方法采用亚甲蓝与人皮肤鳞状细胞癌A431细胞株共同孵育,经630 nm红光照射。采用CCK-8法检测细胞生长的抑制率,确定培育时间、亚甲蓝浓度对A431细胞株的抑制作用。采用免疫组化法检测A431细胞在亚甲蓝光动力作用前后细胞色素c(Cyt-c)在细胞内的分布情况。最后,采用Western-blot方法检测亚甲蓝光动力治疗后细胞凋亡过程中,线粒体凋亡途径相关蛋白Bcl-2和Bax的表达情况。结果CCK8法检测结果显示亚甲蓝光动力组对A431细胞具有杀伤作用,其杀伤作用与亚甲蓝的药物浓度以及亚甲蓝光动力作用后的孵育时间具有密切相关。Cyt-c免疫荧光结果显示亚甲蓝光光动力作用后,细胞凋亡率空白对照0%,光照对照组8%,亚甲蓝对照组12%,光照后1 h 22%,光照后2 h 26%,光照后4 h 31%,光照后6 h 54%。Western blot的检测结果显示亚甲蓝光动力作用激活A431细胞线粒体凋亡途径,启动Bcl-2家族参与凋亡过程。随着亚甲蓝光动力作用后孵育时间的延长,促凋亡蛋白Bax上调,抗凋亡蛋白Bcl-2下调。结论亚甲蓝光动力能明显抑制A431细胞的增殖,并诱导其发生凋亡。凋亡的发生与启动细胞色素c相关的线粒体凋亡途径相关。
