To improve egg production performance of local chicken breed in Guizhou Province, Libo Yaoshan chicken, with dopamine receptor 2 （ DRD2 ） as one of the candidate genes, we detected its genetic variation in 196 Libe ...To improve egg production performance of local chicken breed in Guizhou Province, Libo Yaoshan chicken, with dopamine receptor 2 （ DRD2 ） as one of the candidate genes, we detected its genetic variation in 196 Libe Yaoshan hens using PCR-SSCP （single-strand conformation polymorphism） and sequencing method, and analyzed the correlation between genetic variation and egg production traits. The results showed that TT and TG genotypes in mRNA SNlX）62 （C→T） loci of the DRD2 gene had extremely significant difference in egg production at 38 weeks age （P 〈0.01 ）, and significant difference in egg weight at 300 days age （P 〈0.05 ）. The single nucleotide polymorphisms （SNPs） mutation induced synonymous mutation of the 312th amino acids （leucine） in DRD2 protein, from L （CTG） to L （TI＇G）. The mRNA SNP962 （C→T） loci had a larger genetic effect on egg production at 38 weeks age, and could be used as a molecular marker in early breeding of Libo Yaoshan chicken.展开更多
To provide a reference basis for reasonable development and utilization of Ampelopsis grossedentata resource and its application in production,we added 0.025%,0.05% and 0.1% dihydromyricetin（DMY）extracted from A.gro...To provide a reference basis for reasonable development and utilization of Ampelopsis grossedentata resource and its application in production,we added 0.025%,0.05% and 0.1% dihydromyricetin（DMY）extracted from A.grossedentata into the basal diets of Yaoshan chickens,and studied the effects of DMY on visceral organ indexes and digestive enzyme activities of 40-day-old healthy Yaoshan chickens.Supplementation of DMY in basal diets influenced visceral organ weight,organ index,length and digestive enzyme activity of small intestine of chickens.Among them,0.05% DMY was the most appropriate volume for supplementation.Compared with control group（without DMY）,0.05% DMY reduced lung weight and index（P〉0.05）,significantly reduced liver weight（P〈0.05）,extremely reduced stomach weight and stomach index（P〈0.01）,increased activity of amylase in small intestine（P〉0.05）,significantly increased length of small intestine and activity of protease（P〈0.05）.Supplementation of 0.05% DMY reduced the visceral organ weight and organ index,enhanced digestion and absorption ability of gastrointestinal tract,thereby improving feed utilization rate and promoting the growth of chickens.展开更多
基金Supported by Breeding and Demonstration Promotion of New Yaoshan Chicken Strains(QNYZ[2014]008)Key Gene Screening of Broodiness Breeding of Yaoshan Chicken(QKHJ[2014]2012)Doctoral Starting up Foundation of Guizhou Institute of Animal Husbandry and Veterinary in 2014:STAT5Gene Mutation Detection of Yaoshan Chicken and Associated Analysis with Egg Production Traits&CDS Region’s Cloning and Differential Expression of ONECUT1 Gene in Yaoshan Chicken and Associated Analysis with Production Traits(QKH LH[2015]7067)
文摘To improve egg production performance of local chicken breed in Guizhou Province, Libo Yaoshan chicken, with dopamine receptor 2 （ DRD2 ） as one of the candidate genes, we detected its genetic variation in 196 Libe Yaoshan hens using PCR-SSCP （single-strand conformation polymorphism） and sequencing method, and analyzed the correlation between genetic variation and egg production traits. The results showed that TT and TG genotypes in mRNA SNlX）62 （C→T） loci of the DRD2 gene had extremely significant difference in egg production at 38 weeks age （P 〈0.01 ）, and significant difference in egg weight at 300 days age （P 〈0.05 ）. The single nucleotide polymorphisms （SNPs） mutation induced synonymous mutation of the 312th amino acids （leucine） in DRD2 protein, from L （CTG） to L （TI＇G）. The mRNA SNP962 （C→T） loci had a larger genetic effect on egg production at 38 weeks age, and could be used as a molecular marker in early breeding of Libo Yaoshan chicken.
文摘To provide a reference basis for reasonable development and utilization of Ampelopsis grossedentata resource and its application in production,we added 0.025%,0.05% and 0.1% dihydromyricetin（DMY）extracted from A.grossedentata into the basal diets of Yaoshan chickens,and studied the effects of DMY on visceral organ indexes and digestive enzyme activities of 40-day-old healthy Yaoshan chickens.Supplementation of DMY in basal diets influenced visceral organ weight,organ index,length and digestive enzyme activity of small intestine of chickens.Among them,0.05% DMY was the most appropriate volume for supplementation.Compared with control group（without DMY）,0.05% DMY reduced lung weight and index（P〉0.05）,significantly reduced liver weight（P〈0.05）,extremely reduced stomach weight and stomach index（P〈0.01）,increased activity of amylase in small intestine（P〉0.05）,significantly increased length of small intestine and activity of protease（P〈0.05）.Supplementation of 0.05% DMY reduced the visceral organ weight and organ index,enhanced digestion and absorption ability of gastrointestinal tract,thereby improving feed utilization rate and promoting the growth of chickens.
